Instruction Manual HI 2216 pH/mV/ISE/°C Bench Meter with Custom Buffers and Interval Logging w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using this instrument. This manual will provide you with the necessary information for correct use of this instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com.
PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any damage, notify your Dealer or the nearest Hanna Customer Service Center. Each instrument is supplied with: • HI 1131B Glass-body Combination pH Electrode with 1 m (3.3') Cable • HI 7662 Temperature Probe • HI 76404N Electrode Holder • pH 4.01 & 7.
FUNCTIONAL DESCRIPTION Front Panel Rear Panel 1) Liquid Crystal Display (LCD). 2) CAL key, to enter and exit calibration mode. 3) CFM/GLP key, to confirm calibration, different values or to display Good Laboratory Practice information. 4) ºC key, to manually increase temperature value or other parameters. ºC key, to manually decrease temperature value or other 5) parameters. 6) SETUP key, to enter/exit SETUP mode.
SPECIFICATIONS –2.0 to 16.0 p H –2.00 to 16.00 p H –2.000 to 16.000 p H ±999.9 mV (ORP) ±2000 mV (ORP) RANGE 0.001 to 19990 p p m –20.0 to 120.0 ºC (-4.0 to 248.0 °F) 0.1 p H, 0.01 p H, 0.001 p H 0.1 mV (±999.9 mV) (ISE &ORP) 1 mV (±2000 mV) (ISE &ORP) 0.001 (to 1.999 p p m ) 0.01 (to 19.99 p p m ) 0.1 (to 199.9 p p m ) 1 (to 1999 p p m ) 10 (to 19990 p p m ) RE SOL UTION 0.1 ºC ±0.1p H, ±0.01p H, ±0.002 p H ACCURACY @ 20°C / 68°F ±0.2 mV (±999.9 mV) ±1 mV (±2000 mV) ±0.5% FS ±0.
OPERATIONAL GUIDE POWER CONNECTION Plug the 12 VDC adapter into the power supply socket. Notes: • This instrument uses non volatile memory to retain the pH, mV, temperature calibrations and all other settings, even when unplugged. • Make sure a fuse protects the main line. ELECTRODE AND PROBE CONNECTIONS For pH or ORP measurements connect an electrode with internal reference to the BNC connector on the back of the instrument.
If measurements are taken successively in different samples, it is recommended to rinse the electrode thoroughly with deionized water or tap water and then with some of the next sample to prevent cross-contamination. The pH reading is affected by temperature. In order to measure the pH accurately, the temperature effect must be compensated for.
RELATIVE mV MEASUREMENTS • Press RANGE until “rEL” message and “mV” tag are displayed for one second. After one second the temperature will be displayed on the secondary LCD and the “mv” tag will blink. The reading displayed by the instrument is equal to the difference between the current mV input value and relative mV offset established in the relative mV calibration.
TEMPERATURE MEASUREMENTS Connect the HI 7662 temperature probe to the appropriate socket and turn the instrument on. Submerse the temperature probe into the sample and allow the reading on the secondary LCD to stabilize. p H CALIBRATION Calibrate the instrument frequently, especially if high accuracy is required. The instrument should be recalibrated: • Whenever the pH electrode is replaced. • At least once a week. • After testing aggressive chemicals.
The custom buffers allow the user to calibrate in a buffer solution different from a standard one. Up to two custom buffers can be set in SETUP menu (see page 28). Each custom buffer value can be changed in a ±1.0 pH window around the set value, during calibration, when it is selected; the “BUFFER pH” tag will blink. The instruments will automatically skip the buffer used during calibration and the buffers which are in a ±0.2 pH window, around one of the calibrated buffers.
• The “ ” tag will blink on the LCD until the reading is stable. • When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink. • Press CFM to confirm calibration. • The calibrated value is then displayed on the primary LCD and the third expected buffer value on the secondary LCD.
• When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink. • Press CFM to confirm the fifth calibration point. • The instrument stores the calibration value and returns to normal measurement mode. FOUR, THREE OR TWO-POINT CALIBRATION • Proceed as described in “FIVE-POINT CALIBRATION” section. • Press CAL after the fourth, third or second calibration point was confirmed.
Clear calibration parameters and proceed with calibration from the current calibration point. The instrument will keep all confirmed values during current calibration. • To clear calibration parameters for all uncalibrated buffers starting with current buffer, press CLR. The calibration will continue from the current point. If this procedure is performed while calibrating in the first calibration point, the instrument returns to measurement mode.
p H BUFFER TEMPERATURE DEPENDENCE The temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lesser degree than normal solutions. During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature. TEMP pH BUFFERS ºC ºF 1. 68 4. 01 6. 86 7. 01 9. 18 10. 01 12. 45 0 32 1.67 4.01 6.98 7.13 9.46 10.32 13.38 5 41 1.67 4.00 6.95 7.10 9.39 10.24 13.18 10 50 1.67 4.00 6.
I S E CALIBRATION It is recommended to calibrate the instrument frequently, especially if high accuracy is required. The ppm range should be recalibrated: • Whenever the ISE electrode is replaced. • When the ion charge is changed in SETUP menu. • At least once a day. • After testing agressive chemicals. • When calibration is expired - “CAL” “INTV” tags blink (if feature is enabled). Due to electrode conditioning time, the electrode must be kept submersed a few seconds to stabilize.
• If necessary, press the ARROW keys to select a different standard value. • The “ ” tag will blink on the LCD until the reading is stable. • When the reading is stable and close to the selected standard, the “READY” tag will be displayed and the “CFM” tag will blink. • Press CFM to confirm calibration. • The calibrated value will be displayed on the primary LCD and the second expected standard value on the secondary LCD. Note: The instrument will automatically skip the standard used for the first point.
Default slope value (mV/decade): –59.16 (monovalent cation) - ion charge is 1 59.16 (monovalent anion) - ion charge is –1 –29.58 (divalent cation) - ion charge is 2 29.58 (divalent anion) - ion charge is –2 100 - ion charge is “undF” • Press CLR during calibration if you want to clear calibration values. The instrument will display “CLR” message and will return to measurement mode. • The calibration needs to be cleared when a new electrode is attached to the meter.
RELATIVE m V CALIBRATION • Press CAL when the instrument is in RELATIVE mV measurement mode. The “mV” and “ ” tags will be displayed. Absolute mV is displayed on the primary LCD and “AbS” message is displayed on the secondary LCD. • When the absolute reading is stable and in measurement range, the instrument asks for confirmation. • If the reading is out of range, “WRONG” tag will be displayed. • Press CFM to confirm the absolute value. The instrument will display “0.
Notes: • When the instrument is not calibrated or calibration is cleared (default values loaded) there is no “expired calibration”, and the display always shows the “CAL” “INTV” tags blinking. • When an abnormal condition in the RTC is detected, the instrument forces the “expired calibration” status. pH CALIBRATION DATA Calibration data is stored automatically after a successful calibration. To view the pH calibration data, press GLP while in measurement mode.
The third pH calibration buffer: The fourth pH calibration buffer: The fifth pH calibration buffer: Notes: • The “OLd” message displayed beside the pH value means that this buffer was not used during last calibration. Press and hold down SETUP if you want to see calibration date (or time, if old calibration was performed on the same day as the current calibration). • For each custom buffer used in calibration, the “BUFFER pH” tag will blink.
• The instrument ID. Notes: • Press GLP to return to measurement mode. • If calibration has not been performed, the instruments display “no CAL” message blinking. ISE CALIBRATION DATA The ISE calibration data is stored automatically. To view ISE calibration data, press GLP when the instrument is in ISE measurement mode. The instrument will display the time (hh:mm) of the last calibration. Use the ARROW keys to scroll through the calibration data: • The date (yyyy.mm.dd).
- or the number of days until the calibration alarm will be displayed. - or if expired (7 days ago). • The instrument ID. Notes: • If a one-point calibration is performed after a two-point calibration, the instrument will keep the old slope. • If “no bUF” message appears on the LCD, the instrument informs you that calibration was performed at only one point. • Press GLP at any moment and the instrument will return to measurement mode.
LOGGING FUNCTION Up to 700 LOG samples can be stored into memory. 200 manually logged records and 500 logging records can be stored in the memory. To select logging type enter SETUP menu. LOGGING THE CURRENT DATA (manual logging) Select the manual logging mode in SETUP menu. To store the current reading into memory press LOG while the instrument is in measurement mode.
LOT LOGGING Select “StAb” (stability logging) or the desired time interval. To start interval logging press LOG key while the instrument is in measurement mode. When the selected interval is reached, the instrument will display the current lot number on the primary LCD line, the record number on the secondary LCD line and the “LOG” tag (see example below: Lot 5 record 7) followed by the number of free records on the corresponding memory space.
No ISE records: Otherwise, the instrument will display the lot number on the primary LCD line, the number of records on the secondary, “LOG” tag and “CFM” blinking. If samples were logged on demand “MAn” will be displayed on the primary LCD and the number of samples logged on the secondary (see example below: manual log, 15 samples logged). Press ARROW keys to select different lot. All the existing lots are displayed. Press CFM to view record information.
• The time (hh.mm.ss) is displayed on the LCD, along with the “TIME” tag. • The date (yyyy.mm.dd) is displayed on the LCD along with the “DATE” tag. • The calibration offset is displayed on the primary LCD and “OFS” message on the secondary LCD. Note: If in ISE RECALL mode, the instruments will display “----” message on the primary LCD. • The calibration slope is displayed on the primary LCD and “SLP” message on the secondary LCD.
The “dEL” message is displayed on the primary LCD and the selected record on the secondary LCD, along with ”LOG” tag. Notes: • The ARROW keys can be used to change the record. • Press SETUP to delete all records/lots. The display will show “dEL” in the primary LCD and “ALL” in the secondary LCD. • Press CFM to confirm delete. • Press RANGE to escape and return to the RCL screen. • If “dEL ALL” option was selected, all the log on demand records or lots are deleted.
SETUP Setup mode allows viewing and modifying the following parameters: • Expired Calibration Alarm • Log Interval • First Custom Buffer • Second Custom Buffer • One-point Calibration Behavior • pH Resolution • Ion Charge • Current Time (hour & minute) • Current Date (year, month & day) • Beep Status • Instrument Id • Temperature Unit To enter SETUP mode press SETUP while the instrument is in measurement mode. Select a parameter with the ARROW keys. Press CAL to change a parameter value.
EXPIRED CALIBRATION ALARM Press CAL when the calibration time-out is displayed. Calibration time-out (“OFF” or “1” to “7” days) and “CFM” will start blinking. Press the ARROW keys to change the calibration time-out value. Press CFM to save the modified calibration time-out value. Press CAL to escape without saving. LOG INTERVAL Press CAL when log interval is displayed. The log interval and “CFM” will start blinking (“MAn” for log on demand, “StAb” log on stability, interval in seconds or minutes).
ONE-POINT CALIBRATION BEHAVIOR Press CAL when “1 Pnt” message is displayed on the secondary LCD. One of the two options (“Pnt” or “OFFS”) and “CFM” will start blinking (see pH CALIBRATION PROCEDURE for details, page 9). Press the ARROW keys to toggle between “Pnt” and “OFFS” options. Press CFM to save the behavior for one-point calibration. Press CAL to escape without saving. pH RESOLUTION Press CAL when “rES” message is displayed on the secondary LCD. The set resolution (“0.1”, “0.01” or “0.
CURRENT TIME Press CAL when the current time is displayed. The hour and “CFM” will start blinking. Press the ARROW keys to change the hour. Press RANGE. The minutes and “CFM” will start blinking. Press the ARROW keys to change the minutes. Press CFM to save the modified value. Press CAL to escape without saving. CURRENT DATE Press CAL when the current date is displayed. The year and “CFM” will start blinking. Press the ARROW keys to change the year. Press RANGE. The month and “CFM” will start blinking.
Press the ARROW keys to change the beep status (On or OFF). Press CFM to save the modified beep status. Press CAL to escape without saving. When enable, beep sounds as a short beep every time a key is pressed or when the calibration can be confirmed. A long beep alert that the pressed key is not active or a wrong condition is detected while in calibration. INSTRUMENT ID Press CAL when the “InId“ is displayed. The instrument ID (“0000“ to “9999“) and “CFM” will start blinking.
TEMPERATURE CALIBRATION (for technical personnel only) All the instruments are factory calibrated for temperature. Hanna’s temperature probes are interchangeable and no temperature calibration is needed when they are replaced. If the temperature measurements are inaccurate, temperature recalibration should be performed. For an accurate recalibration, contact your dealer or the nearest Hanna Customer Service Center, or follow the instructions below.
• Use the ARROW keys to set the reading on the secondary LCD to that of the hot water. • When the reading is stable and close to the selected calibration point, “READY” tag will appear and “CFM” tag will blink. • Press CFM to confirm. The instrument memorize calibration and restart to measurement mode. Note: If the reading is not close to the selected calibration point, “WRONG” tag will blink. Change the temperature probe and restart calibration.
Notes:• If the reading is not close to the selected calibration point, “WRONG” tag will blink. Verify calibration condition or contact your vendor if you can not calibrate. • Pressing CAL key during temperature or mV calibration the instrument quit calibration mode and restart to measurement mode without memorizing calibration. PC INTERFACE Data transmission from the instrument to the PC can be done with the HI 92000 Windows® compatible software (optional).
CHR xx Change the instrument range according with the parameter value (xx): • xx=00 pH range/0.001 resolution • xx=01 pH range/0.01 resolution • xx=02 pH range/0.
MDR GLP • Meter status (2 chars of status byte): represents a 8 bit hexadecimal encoding. • 0x10 - temperature probe is connected • 0x01 - new GLP data available • 0x02 - new SETUP parameter • Reading status (2 chars): R - in range, O - over range, U - under range. First character corresponds to the appropriate range reading. Second character corresponds to mV reading. • Primary reading (corresponding to the selected range) - 7 ASCII chars, including sign and decimal point.
PAR NSLx • the calibration time, yymmddhhmmss (12 chars). • electrode condition, with sign (3 chars). The “-01” code means not calculated. • Rel mV calibration data (if available), which contains: • the calibration offset, with sign (7 chars) • the calibration time, yymmddhhmmss (12 chars).
LODPxxx Requests the xxxth pH record logged data. LODMxxx Requests the xxxth mV/Rel mV record logged data. LODIxxx Requests the xxxth ISE record logged data.
ELECTRODE CONDITIONING & MAINTENANCE PREPARATION PROCEDURE Remove the protective cap of the pH electrode. DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode. These bubbles can be removed by “shaking down” the electrode as you would do with a glass thermometer.
For refillable electrodes: If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI 7082 or HI 8082 3.5M KCl Electrolyte Solution for double junction or HI 7071 or HI 8071 3.5M KCl+AgCl Electrolyte Solution for single junction electrodes. For faster response, unscrew the fill hole screw during measurements. For AMPHEL® electrodes: If the electrode does not respond to pH changes, the battery is run down and the electrode should be replaced.
CLEANING PROCEDURE Soak in Hanna HI 7061 or HI 8061 General Cleaning Solution for approximately ½ hour. • Protein Soak in Hanna HI 7073 or HI 8073 Protein Cleaning Solution for 15 minutes. • Inorganic Soak in Hanna HI 7074 Inorganic Cleaning Solution for 15 minutes. • Oil/grease Rinse with Hanna HI 7077 or HI 8077 Oil and Fat Cleaning Solution.
TROUBLESHOOTING GUIDE SYMPTOMS PROBLE M SOLUTION Slow response/excessive D irty pH electrode. drift. C l e a n t h e e l e c t r od e a n d t h e n s oa k t h e t i p i n HI 7061 or HI 8061 for 30 minutes. Readings fluctuate up Clogged/dirty junction. and down (noise). Low electrolyte level (refillable electrodes only). Clean the electrode. Refill with fresh solution (for refillable electrodes only). Check cable and connectors. The meter does not accept the buffer solution for calibration.
TEMPERATURE CORRELATION FOR p H SENSITIVE GLASS The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher. In addition, the response time will suffer to a greater degree at temperatures below 25 ºC. Since the resistance of the pH electrode is in the range of 50 – 200 Mohm, the current across the membrane is in the pico Ampere range.
ACCESSORIES pH BUFFER SOLUTIONS HI 70004P HI 70007P HI 70010P HI 7001L HI 7004L HI 7006L HI 7007L HI 7009L HI 7010L HI 8004L HI 8006L HI 8007L HI 8009L HI 8010L pH 4.01 Buffer Sachets, 20 mL, 25 pcs pH 7.01 Buffer Sachets, 20 mL, 25 pcs pH 10.01 Buffer Sachets, 20 mL, 25 pcs pH 1.68 Buffer Solution, 500 mL pH 4.01 Buffer Solution, 500 mL pH 6.86 Buffer Solution, 500 mL pH 7.01 Buffer Solution, 500 mL pH 9.18 Buffer Solution, 500 mL pH 10.01 Buffer Solution, 500 mL pH 4.
ORP PRETREATMENT SOLUTIONS HI 7091L Reducing Pretreatment Solution, 500 mL HI 7092L Oxidizing Pretreatment Solution, 500 mL pH ELECTRODES All electrodes part numbers ending in B are supplied with a BNC connector and 1 m (3.3') cable, as shown below : HI 1043B Glass-body, double junction, refillable, combination pH electrode. Use: strong acid/alkali. HI 1053B Glass-body, triple ceramic, conic shape, refillable, combination pH electrode. Use: emulsions.
HI 1330B Glass-body, semimicro, single junction, refillable, combination pH electrode. Use: laboratory, vials. HI 1331B Glass-body, semimicro, single junction, refillable, combination pH electrode. Use: flasks. HI 1230B Plastic-body (PES), double junction, gel-filled, combination pH electrode. Use: general, field. HI 2031B Glass-body, semimicro, conic, refillable, combination pH electrode. Use: semisolid products. HI 1332B Plastic-body (PES), double junction, refillable, combination pH electrode.
FC 100B Plastic-body (PVDF), double junction, refillable, combination pH electrode. Use: general purpose for food industry. FC 200B Plastic-body (PVDF), open junction, conic, Viscolene, non-refillable, combination pH electrode. Use: meat & cheese. FC 210B Glass-body, double junction, conic, Viscolene, non-refillable, combination pH electrode. Use: milk, yogurt. FC 220B Glass-body, triple-ceramic, single junction, refillable, combination pH electrode. Use: food processing.
HI 1413B Glass-body, single junction, flat tip, Viscolene, non-refillable, combination pH electrode. Use: surface measurement. ORP ELECTRODES HI 3131B Glass-body, refillable, combination platinum ORP electrode. Use: titration. HI 3230B Plastic-body (PES), gel-filled, combination platinum ORP electrode. Use: general purpose. HI 4430B Plastic-body (PES), gel-filled, combination gold ORP electrode. Use: general purpose.
EXTENSION CABLE FOR SCREW-TYPE ELECTRODES (SCREW TO BNC ADAPTER) HI 7855/1 Extension cable 1 m (3.3') long HI 7855/3 Extension cable 3 m (9.
RECOMMENDATIONS FOR USERS Before using these products, make sure they are entirely suitable for the environment in which they are used. Operation of these instruments in residential areas could cause unacceptable interferences to radio and TV equipment, requiring the operator to follow all necessary steps to correct interferences. The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges. Avoid touching this glass bulb at all times.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.
