DOC022.53.00654 DR 5000 USER MANUAL January 2008, Edition 2 © HACH Company, 2007–2008. All rights reserved. Printed in Germany.
Table of Contents Section 1 Specifications ........................................................................................................................................ 7 Section 2 General Information .............................................................................................................................. 9 2.1 Safety information .............................................................................................................................................. 9 2.
Table of Contents 5.4 Stored Programs .............................................................................................................................................. 42 5.4.1 Select a saved test/method; enter user-specific basic data .................................................................... 42 5.4.2 Stored program options .......................................................................................................................... 42 5.4.3 Use of program timers ........
Table of Contents 6.4 Single Wavelength (absorbance, concentration and transmittance measurements) ....................................... 87 6.4.1 Set up single wavelength mode .............................................................................................................. 87 6.4.2 Take single wavelength measurements (single reading) ........................................................................ 89 6.4.3 Take single wavelength measurements (continuous readings) .....................
Table of Contents 6
Section 1 Specifications Specifications are subject to change without notice. Performance specifications Operating mode Transmittance (%), Absorbance and Concentration Source lamp Gas-filled Tungsten (visible) and Deuterium (UV) Wavelength range 190–1100 nm Wavelength accuracy ± 1 nm in Wavelength Range 200–900 nm Wavelength reproducibility < 0.1 nm Wavelength resolution 0.
Specifications 8
Section 2 General Information 2.1 Safety information Please read this entire manual before unpacking, setting up or operating this equipment. Pay attention to all danger, warning and caution statements. Failure to do so could result in serious injury to the operator or damage to the equipment. Make sure that the protection provided by this equipment is not impaired, do not use or install this equipment in any manner other than that specified in this manual. 2.1.
General Information 2.1.3 Class 1 LASER A Class 1 LASER is installed in this instrument. Class 1 LASERS are products where the radiant power of the LASER beam accessible (the accessible emission) is always below the Maximum Permissible Exposure value. Therefore, for Class 1 LASERS the output power is below the level at which it is believed eye damage will occur. Exposure to the beam of a Class 1 LASER will not result in eye injury. Class 1 LASERS may therefore be considered safe.
General Information 2.2 Overview of product The DR 5000 Spectrophotometer is a complete scanning UV/VIS spectrophotometer with a wavelength range of 190 to 1100 nm. The instrument comes with a complete set of application programs and multi-language support. The DR 5000 Spectrophotometer contains the following application modes: Stored Programs, User Programs, Favorite Programs, Single Wavelength Mode, Multi-Wavelength Mode, Wavelength Scanning Mode and Time Course Mode.
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Section 3 Installation WARNING Electrical and Fire Hazards. Use only the provided power supply. Only qualified personnel should conduct the tasks described in this section of the manual. 3.
Installation 3.3 Connection The DR 5000 has three USB interfaces as a standard feature, located on the back of the instrument (Figure 1). The USB Type A interfaces are used for communications with a printer, USB memory stick or keyboard. A USB memory stick is used to update instrument software. The USB Type B interface is used for communications with a PC. The optional Hach Data Trans software (see section 5.2.6.3 on page 31) must be installed on the PC for this use.
Installation 3.4 Cell compartment, Multi Cell Holder, 100 mm Cell Adapter (optional) Figure 2 Cell compartment 1 Cell compartment 2 Cell compartment for 13 mm or 16 mm round cuvettes/vials only The DR 5000 Spectrophotometer comes equipped with a Multi Cell Holder (Figure 3 on page 16) which is the Standard Holder, provided with each instrument.
Installation Figure 3 Multi Cell Holder (Top and Bottom) 1 10 mm square cell 4 1“ round cell 2 20 mm rectangular cell 5 1“ square cell 3 50 mm rectangular cell 3.4.
Installation 3.4.2 Installation Multi Cell Holder Assembly of the Multi Cell Holder: 1. Open the cell compartment. 2. Identify the correct opening for the selected cell type in the Multi Cell Holder. 3. Insert the Multi Cell Holder in the cell compartment in such a way that the name of the selected cell type can be read directly and the cell opening is at the front. Secure the holder with two locking screws (Figure 5).
Installation 3.
Section 4 Start Up 4.1 Power the instrument on and off 1. Plug in power supply. 2. Close the empty cell compartment. 3. Turn the instrument on by pressing the power switch on the back. Note: Do not turn the instrument off and on in rapid succession. Always wait about 20 seconds before turning the instrument on again, otherwise the electronic and mechanical systems will be damaged. 4.2 Language selection The spectrophotometer software includes several language options.
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Section 5 Standard Operations 5.1 Overview 5.1.1 Tips for the use of the touch screen The entire screen is touch-activated. To make a selection, press the screen with a fingernail, fingertip, pencil eraser or a stylus. Do not press the screen with a sharp object, such as the tip of a ball point pen. • Do not place anything on top of the screen, to prevent damage or scratching on the screen. • Press keys, words or icons to select them. • Use scroll bars to move up and down long lists very quickly.
Standard Operations 5.1.3 Main Menu A variety of modes may be selected from the Main Menu. The following table briefly describes each menu option. Table 2 Main Menu options Option Function Stored Programs (HACH LANGE Programs) Stored programs are pre-programmed methods that make use of HACH reagents and LANGE cuvette tests and pipette tests. The DR 5000 Procedures Manual contains illustrated, step-by-step procedures for analyses using HACH programs.
Standard Operations 5.2 Instrument Setup mode 1. Select Instrument Setup in the Main Menu. A selection of functions appears in order to configure the functions of the instrument. This display appears when the multi-cell holder is installed or the 13 mm round cuvette/vial compartment is used. If the imstrument recognizes a Sipper module or a Peltier module the Carousel Options key is replaced by Sipper Options or Peltier Options.
Standard Operations 5.2.2 Sample ID Use this option to enter up to 30 Sample Identification tags (up to 13 characters each) into the instrument. Sample IDs can be used to specify the sample location or other sample specific information. 1. Press Sample ID in the Instrument Setup. 2. Press New to enter a new Sample ID. 3. Use the alphanumeric keypad to enter a new Sample ID. Note: If a USB Barcode handset scanner ( see Section 9 on page 129) is connected, Sample IDs can also be scanned. 4.
Standard Operations There are two ways to enter or change a Sample ID in the Measurement Mode: Option 1 In the result display, press Options>More>Instrument Setup. If a Sample ID is already assigned, select the Sample ID symbol in the result display. Option 2 With an USB keyboard (with US keyboard layout) or a scanner a Sample ID can be directly entered in the result display. 5.2.3 Date and time 1. Press Date & Time in the Instrument Setup. 2. The date and time are subdivided over a number of fields.
Standard Operations 2. Select Long to change the number of audio signals. Use the alphanumeric keypad to enter/specify the number of audio signals (4–25). Note: A high number of audio signals increases the duration of the tones and a small number of audio signals reduces the duration of the tones. 3. Press OK to confirm. The selected number of the audio signals sounds as a corresponding acoustic signal. 4. Press OK to confirm. The instrument will return to Instrument Setup. 5.2.
Standard Operations 5. Select the length of time the lamp will be switched on. Note: After this period of time the lamp automatically turns off, after no measurement has been made outside the corresponding range (UV/VIS). 6. Touch OK to confirm. 7. Touch UV Switch key to select the wavelength value between 320 nm and 360 nm, at which the instrument changes from the visible to UV source. 8. Use the alphanumeric keypad to enter the maximum wavelength for UV operation. 9.
Standard Operations When the lamp is warmed up and ready, the UV icon stops flashing. Note: If both lamps are On, the icon UV-VIS is displayed in the selected Measurement Mode. Note: Alternatively you can touch the UV- or VIS-Lamp icon on the measurement screen to change the actual setting.
Standard Operations 5.2.6 PC and printer The instrument is provided with 3 USB interfaces, which are located on the back of the instrument (see Figure 1 on page 14). These interfaces can be used for exporting data and graphics to a printer, updating data and for data communication to a personal computer. These interfaces can be used for the connection of a USB stick, an external USB keyboard or a USB Barcode handset scanner. Note: A USB hub may be used to connect several accessories at a time.
Standard Operations Printer Setup: • Resolution: Print quality • Paper: Paper size Note: If an optional Thermal Printer is connected, the function "Auto Send" on/off is available. 4. Select Auto-Send: On to send all measured data automatically to the Thermal printer. Note: The option Auto-Send is not available for any other printer (e.g. ink jet printer). 5. Press Resolution to select the print quality. Select between • 100 dpi • 150 dpi and • 300 dpi 6. Press OK to confirm.
Standard Operations 7. Press Paper to select the paper size. Select between • Letter • Legal • Executive • A4 8. Press OK to confirm. Note: Press OK again to return to the PC & Printer menu. 5.2.6.2 Print data 1. Press Recall Data in the Main Menu. 2. Select the data source, where the data to be printed are stored. 3. A list is displayed. Data can be filtered. For more information see section 5.3.1.2 on page 35. 4.
Standard Operations 4. Select Auto-Send: On to send all measured data automatically to the PC. Note: If Auto-Send: Off is selected, the PC & Printer icon must be pressed, in order to send data to the PC. Note: The remote function is only for monitoring the data transfer. 5.2.7 Password The Password menu contains a variety of security settings to control access to various functions. For example, prevent unauthorized changes to stored programs or instrument configurations. 1.
Standard Operations 4. Press Security List to lock various functions for unauthorized users. 5. Highlight the desired functions to control. 6. Confirm the Security List with OK to return to the Password menu. 7. Press On to highlight the new settings of the Security List. 8. Enter the new Password again to confirm. 9. Press OK to return to Instrument Setup. Note: The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting. 5.2.7.1 Password deactivation 1.
Standard Operations 3. Press Off to deactivate the settings of the Security List. 4. Press OK to return to Instrument Setup. Note: Use this function to delete the former Password or to enter a new one.
Standard Operations 5.3 Store, recall, send and delete data 5.3.1 The Data Log The Data Log will store up to 1000 readings taken in the modes: Stored Programs, User Programs, Favorite Programs, Single Wavelength and Multi Wavelength. A complete record of the analysis is stored, including the Date, Time, Results, Sample ID, and Operator ID. 5.3.1.
Standard Operations 4. The function Filter Settings is used to search for specific items. 5. Highlight On to turn on the filters to select data by • Sample ID • Operator ID • Start Date • Parameter or any combination of the four. 6. Press OK to confirm the selection. The chosen items are listed. 7. Press View Details to get more information. 5.3.1.3 Send data from the data log Data is sent from the data log as CSV (Comma Separated Value) files through a USB memory stick to a file named DATALOG.
Standard Operations 5.3.1.4 Delete stored data from the data log 1. Press Recall Data in the Main Menu. 2. Press Data Log>Options>Delete. 3. Highlight Single Point or Filtered data or All data and press OK to confirm. Note: The number in parentheses is the total number of data sets assigned to this selection.
Standard Operations 5.3.2 Store, recall, send and delete data from wavelength scan and time course The instrument can store 20 Wavelength Scans and 20 Time Course Data sets.The data can be stored manually at the user's discretion after viewing the data. 5.3.2.1 Data storage from wavelength scan or time course 1. Press the Store icon in the Options menu after a reading is taken. The Store Data list will be displayed. 2. Press Store to save the current scan to the highlighted numbered line.
Standard Operations 2. Press Graph to look at details. Note: Press View Summary to return to the Recall Data list. 3. Press Table to look at details. Note: Press View Summary to return to the Recall Data list. 5.3.2.3 Send data from wavelength scan or time course There are two ways to recall sent data to a USB memory stick, printer or PC with Hach Data Trans. Option 1: 1. Press Recall Data in the Main Menu and then Wavelength Scan or Time Course. 2.
Standard Operations • When a printer is connected, select how to send the data to the printer (graph, table or both graph and table). • When a USB memory stick is connected, the files will be automatically sent as CSV files (Comma Separated Value) to a file ”WLData“ (Wavelength Scan Data) or ”TCData“ (Time Course Data) to the USB memory stick. The file name will be formatted as: ”ScanData_X.csv“ (Wavelength Scan Data) or ”TCData_X.csv” (Time Course Data).
Standard Operations 5.3.2.4 Delete stored data from wavelength scan or time course 1. Press Recall Data from the Main Menu and then Wavelength Scan or Time Course or Options>More>Recall Data. A listing of the stored data is displayed. 2. Highlight any data to delete. 3. Press Delete in the Options menu and press OK to confirm.
Standard Operations 5.4 Stored Programs The instrument contains more than 200 programmed procedures. They can be accessed through the Stored Programs menu. 5.4.1 Select a saved test/method; enter user-specific basic data 4. Press Stored Programs in the Main Menu to view an alphabetical list of stored programs with program numbers. The Stored Programs list will appear. 5. Highlight the required test.
Standard Operations Table 4 Stored programs options (continued) Options Description Store Off/On With the Store On setting, all measurement data are stored automatically. With the Store Off setting, no measurement data are stored. % Trans/Conc/Abs To switch to % transmittance, concentration or absorbance readings Send Data icon / Send Data To send Data to a printer, computer or USB memory stick (Type A) Timer icon This functions as a stopwatch.
Standard Operations 5.4.3 Use of program timers Some procedures do not require the use of timers. Other procedures require several timers. These timers are pre-programmed into each Stored Program, along with a description of the activity to be performed during the timed period. 1. Press the Timer icon on the display. 2. Press OK to start the first timer. The timer will count down on the screen. 3. To start the next timed activity for the Stored Program, press the Timer icon and OK.
Standard Operations 5.4.4.1 Take single wavelength measurements (single reading) 1. Insert the blank cuvette into the cuvette/sample cell holder. Press Zero. Note: The Read key is only active after the zero measurement has been carried out. 2. Insert the sample cuvette into the cuvette/sample cell holder. Press Read. Note: For data storage, see section 5.3.2 on page 38. 5.4.4.2 Take single wavelength measurements (continuous readings) 1. Insert the blank cuvette into the cuvette/sample cell holder.
Standard Operations 3. Press Return to return to the result display. 4. Confirm ’Store present reading again?’ to save the current measured value with the dilution factor. Note: When a dilution is in effect, the dilution icon will appear on the display. Note: If using undiluted samples, set the dilution factor off again. 5.4.6 Run a standard adjust The Standard Adjust function allows the calibration curve for a stored program to be adjusted based on analysis of a known standard solution.
Standard Operations 5. Press Adjust to enable the Standard Adjust. The Standard Adjust icon will appear. Note: The adjustment must be within certain limits, which vary with each program. The allowable percentage is shown after "Adjustment“. Note: When a Standard Adjust is in effect, the Standard Adjust icon will appear on the display. 5.4.7 Set the chemical form Some Stored Programs allow a variety of chemical forms to be selected. Press the unit (e.g.
Standard Operations 5.4.8 Run a reagent blank Some of the stored tests/methods include the "Reagent Blank" function. This enables the reagent blank value to be measured and then taken into account in calculating the measurement result. Measurement/analysis of a reagent blank: 1. Prepare the test/method in accordance with the (working) procedure. Instead of a sample, deionized water is used to determine the reagent blank value. 2. Select the test.
Standard Operations 5.4.9 Analysis of samples 1. Press Stored Programs and select a program. 2. Insert the blank cuvette/cell into the cuvette/sample cell holder. 3. Press Zero. 4. Remove blank cuvette/cell and insert sample cuvette/cell into the cell compartment. 5. Press Read. The result will be displayed. 6. For data storage, see section 5.3.2 on page 38. 5.4.
Standard Operations 5.5 Barcode Programs A special barcode reader in cell compartment #1 automatically reads the barcode on the 13 mm cuvette/vial as the cuvette/vial completes a single rotation. The instrument uses the barcode identification to automatically set the correct wavelength for the analysis and calculates the result immediately with the help of the stored factors. In addition, measured values are recorded at 10 different positions during a rotation.
Standard Operations 5.5.1 Complete a barcode 13 mm test/vial Note: The cell compartment must be closed before a measurement is made! 1. The instrument needs to be at the Main Menu. 2. Prepare the barcode 13 mm cuvette/vial in accordance with the working procedure and insert the cuvette/vial in cell compartment #1 and close the cell compartment. When a coded cuvette/vial is placed in cell compartment #1 (Figure 7), the corresponding measurement program is automatically activated in the Main Menu.
Standard Operations 1. In the result display, press Options>More>Chemical Form. A list of available evaluation forms appears. 2. Select the required chemical form and press OK to confirm. Note: The selected chemical form is displayed, but does not become the default. To change the default, see section 5.5.3.1. 5.5.3.1 Change of the default setting of the chemical form 1. Insert the blank or sample cuvette/vial (depending on the working procedure) into the cell compartment and close the cell compartment.
Standard Operations Table 5 Barcode program options (continued) Options Description Send Data icon / Send Data To send data to a printer, computer or USB memory stick (Type A) Timer icon This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed (e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement program.
Standard Operations procedure. Other barcode tests use the reagent test vials to determine the sample blank. Please refer to the Hach procedure for method-specific instructions. 5.5.6 Update/edit barcode tests 5.5.6.1 Manual update of a barcode test Using the data provided in the barcode, the instrument automatically sets the measurement wavelength and factors. If a discrepancy is detected between the barcode data and the stored data or a new test is identified, the instrument requests an update.
Standard Operations Enter the Version 1. Highlight the line containing Version and press Edit. Use the alphanumeric keypad to enter the name indicated in the working procedure. 2. Press OK to confirm. Enter the Measurement Process For detailed information on entering the measurement process, see section 6.1.2 on page 72. Check the working procedure in advance to determine whether this point needs to be changed. 1. Highlight the Measurement Process line in the data overview and press Edit. 2.
Standard Operations Enter the Variables (Factors, Wavelength and Conversion Factors) For detailed information on entering the Variables, see section 6.1.2 on page 72. Check the working procedure in advance to determine whether this point needs to be changed. 1. Highlight the Variables line in the data overview and press Edit. 2. Select the variable, press Edit and use the alphanumeric keypad to enter the data specified in the working procedure (for F1, F2, λ1, U1 etc.). Confirm each entry with OK.
Standard Operations 5.5.6.2 Update an existing barcode test Note: Only in very few cases does the revision of a test require all test specifications to be updated. Option 1: Manual update of test data 1. The instrument needs to be at the Main Menu. 2. Insert the blank or sample cuvette/vial (depending on the working procedure) in cell compartment #1 and close the cell compartment. 3.
Standard Operations 5.5.6.3 Program a new test 1. The instrument needs to be at the Main Menu. 2. Insert the sample cuvette/vial or blank (depending on the working procedure) into the cell compartment #1 and close the cell compartment. 3. After "Program not available", a data overview is displayed, including the specifications of the test that is to be revised.
Standard Operations 6. Press OK to return to the System Checks menu. Note: When the instrument software has been updated, a prompt to restart the instrument is displayed.
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Section 6 Advanced Operations 6.1 User Programs User programs provide the opportunity to complete "made to measure" analysis. The User Programs database is empty when the instrument leaves the factory and is used to accommodate programs created by users specific needs. Here are a few examples of entries: • Programming of user-created procedures. The analysis procedure must be developed first, before it can be programmed.
Advanced Operations Program Number: Specific test number, with which the program can subsequently be called up from the selection list in the User Programs menu or the Favorites menu. 2. Use the alphanumeric keypad to enter a program number between 950 and 999. The lowest available number appears automatically. 3. Press OK. Note: If the program number is already assigned to another user program, a message appears, asking whether the existing program should be replaced.
Advanced Operations 6.1.1.1 Single wavelength settings If the Single Wavelength mode is selected, the following parameters can be defined: Units: Select the required unit from the list and press Next. Note: Units of measure not included in this list can be added in the edit program under Program Options, Edit. Select Units, Edit and then New. Wavelength (single wavelength program type): Use the alphanumeric keypad to enter the measurement wavelength.
Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter. Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.2 Multi wavelength settings If the Multi Wavelength mode is selected, the following parameters can be defined: Units: Select the required unit from the list and press Next. Note: Units of measure not included in this list can be added in the edit program under Program Options, Edit.
Advanced Operations List of available absorbance formulas A1 is the absorbance at wavelength 1, A2 is the absorbance at wavelength 2 and so on K1 is the factor at wavelength 1, K2 is the factor at wavelength 2 and so on If a subtraction has to be completed, the factors can be entered with a minus sign. Wavelength λx: Press a λx key and use the alphanumeric keypad to enter a wavelength. Press another λx key and enter the next wavelength.
Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter. Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.3 Calibration settings for single and multi wavelength mode A method is calibrated by determining the absorbance values of several standard solutions of known concentration. There are three ways to create and store a calibration curve. Instructions for each method follows (Table 9).
Advanced Operations 2. To enter the standard concentrations and corresponding absorbance values in the displayed table, press the "+" symbol. Use the alphanumeric keypad to enter the values. Press OK and enter the corresponding absorbance value. Press OK. The entered data are displayed in the table. Repeat the sequence for each data point to enter it. 3. To change a value in the table, highlight the appropriate line, press the unit key (e.g.
Advanced Operations Calibration by reading standards 1. Press Read Standards and press Next. 2. To enter the standard concentrations in the displayed table, press the "+" symbol. Use the alphanumeric keypad to enter the standard concentration. Press OK. 3. Press the "+" symbol again (see arrow) and enter the next standard concentration. Repeat this sequence until all standard conentrations (maximum of 24 solutions) have been entered. 4.
Advanced Operations 9. Press Force 0 to change the setting from Off to On. The curve then passes through the origin of the coordinate system. Note: This may have an adverse effect on the correlation coefficient (r2). 10. Press Table to display the table again. 11. When the table has been completed and the curve type has been chosen, press Done when the graph is displayed or Exit when the table is displayed. Go to section 6.1.1.4 on page 70. Calibration by entering the formula 1.
Advanced Operations 6.1.1.4 Store a user program The input of the basic data is complete. An overview of the variable program data is displayed. 4. To enter more specifications or change existing ones, highlight the appropriate line and press Edit. 5. Select Store to save the user program. 6. Press the PC & Printer icon to send the program data to a printer or to a USB memory stick (connect the USB memory stick to the USB interface first).
Advanced Operations Timer 1 / Timer 2 / Timer 3 / Timer 4: This function can define time intervals for up to four timers. Timer designations such as Shake, Wait and Swirl can be assigned. 1. Highlight the appropriate line in the overview of the program data and press Edit. 2. The timers are activated or deactivated with the check boxes in the left part of the display. In the next column, a selection can be made from a list of names that designate the corresponding work step.
Advanced Operations 6.1.2 Free programming program type Free Programming is an advanced option for entering original user-developed methods. When the Free Programming option is selected, an overview of the specifications of the programmed test is displayed. Each input option can be modified to develop the user method. Refer to Table 10 for more information. To modify an input option, select the appropriate line and press Edit. Important Note: Perform steps 1–6 of Programming a New User Method (section 6.1.
Advanced Operations • Should individual program sequences be repeated? The elements of a measuring sequence, such as zero and sample measurements and the timer(s) (reaction times, waiting times, etc.) are individually defined. 6.1.2.2 Enter a new element of a measuring sequence Important Note: Each component of the measurement process must be entered in the order in which it will be completed. 1. Highlight the Measurement Process line in the data overview and press Edit. 2.
Advanced Operations Process Timer key 1. Press the Process Timer key to enter any waiting, reaction or handling times that have to be taken into account. Use the alphanumeric keypad to enter the time. Press OK and confirm the input by pressing OK again. Note: This time is integrated into the measurement process. Note: The entered measurement sequence is displayed. [R] key / Reading... 1. Press the [R] key to program a measurement of the substance that is to be analyzed. Confirm with OK. 2.
Advanced Operations Delete an element of a measuring sequence Select the appropriate line and press Delete. The element is deleted. Insert an element of a measuring sequence Select the line in the measuring sequence where the insertion is be made and press New. A new element can be entered at the selected position. When the input is complete, press OK in the "Measurement Process" display. The data overview is then displayed. 6.1.2.
Advanced Operations Refer to Table 11 for detailed information on the Edit formula keys. Note: The evaluation formula is built up successively in the display in accordance with the input. Note: The arrow key deletes the most recently entered element of the formula.
Advanced Operations Table 11 Edit formula key descriptions (continued) Screen Key +– ÷x >=< Description Press +– ÷x, to enter a mathematical operation. Select the operation and press OK to confirm. The available choice of mathematical operations depends on the defined formula. This means that functions such as "( )" or "ln"/"log" etc.
Advanced Operations Select Conc. key If an already defined formula, in this case C1, is to be taken into account in the formula for C2, press Select conc. Enter the number of the formula (e.g. 1 for C1) and press OK to confirm. Cn can now be linked with a mathematical operation. Note: The Cn concentrations that are to be calculated are numbered in sequence: C1, C2, C3, etc. Note: When the first Cn formula has been defined, the Formula list is automatically extended by Cn+1. 6.1.2.4 Enter variables 1.
Advanced Operations 6.1.3 Select a user program 1. Press User Programs in the Main Menu to view an alphabetical list of user programs with program numbers. The User Programs list will appear. Note: Use the scroll bar to scroll through the list quickly. Note: Highlight the selection by pressing it or press Select by Number to search for the program by number. 2. Press Start to run the program. 6.1.
Advanced Operations 6.1.4.1 Add to Favorites 1. Press Add to Favorites and press OK to confirm. The program is added to the Favorites. 6.1.4.2 Edit 1. Press Edit and press OK to confirm. An overview of the specifications of the programmed test is displayed. More information about the input options is provided in the section 6.1.2 on page 72. 6.1.4.3 Delete 1. Press Delete and press OK to confirm. The program is deleted from the list of User Programs.
Advanced Operations 6.2 Favorite Programs The most frequently used tests/methods in the Stored Programs menu and the User Programs menu can also be added to the list of favorites to simplify their selection. To add Stored Programs and/or User Programs to the favorites list or the favorite programs, see section 6.1.4 on page 79. 6.2.1 Recall a favorite program 1. Press Favorite Programs in the Main Menu. The Favorite Programs list will appear. 2. Highlight the selection by pressing it.
Advanced Operations 6.3 Standard Addition – monitoring/checking results The accuracy of measured values (their correspondence with the actual concentration of the analyte in the sample) and their precision (correspondence of the measurement results obtained from several samples containing the same concentration of the test analyte) can be determined or improved using the standard addition method. This method (also referred to as spiking) serves to identify sample-specific interference factors, e.g.
Advanced Operations e. Does the test require the sample to be at a certain temperature? f. Was the pH of the sample in the correct range? g. Is the pipette volume correct? 2. Check the used reagents by repeating the standard addition procedure with freshly prepared reagents. Detection rate Conclusion 100% The originally used reagents were defective. Check the standard solution: Repeat the standard addition procedure with a freshly prepared standard solution.
Advanced Operations Peak volume/sample volume methods 1. Select Stored Programs in the main menu. Select the required program. 2. Press Start. 3. Analyze a sample without added standard solution in accordance with the instructions in the Procedures Manual. When the measurement is complete, leave the sample cuvette/cell in the cuvette/sample cell holder. 4. Press Options>More>Standard Addition. An overview of the data of the standard addition procedure is displayed. 5.
Advanced Operations 6. Press the key of the value to change it. Use the alphanumeric keypad to change the value. Press OK to confirm. 7. Press the keys to enter the standard addition volumes. Use the alphanumeric keypad to enter the new data and press OK. Description of the table of readings • The first column shows the standard addition volume. 0 mL stands for a sample containing no added standard. • The second column shows the reading of the samples with and without added standard.
Advanced Operations 11. After all the standard addition solutions have been measured, press Graph. The regression line through the standard addition data points is displayed. The correlation coefficient r2 indicates how close the data points are to the line. If the correlation coefficient = 1, the curve is linear. The concentration shown above the curve is the estimated concentration of the sample without the added standard. Note: In the curve menu, the name on the Curve key switches to Table.
Advanced Operations 6.4 Single Wavelength (absorbance, concentration and transmittance measurements) The Single Wavelength mode can be used in three ways. For sample measurements at a single wavelength, the instrument can be programmed to measure the absorbance, % transmittance or concentration of the analyte. Absorbance measures the amount of light absorbed by the sample, in units of absorbance.
Advanced Operations Table 12 Single wavelength setup options (continued) Options Description Reading Mode Single Reading Mode: A reading is only displayed after a measurement has been carried out (press Read; standard setting) (see section 6.4.2 on page 89). Continuous Reading Mode: After the zero measurement, all readings are displayed automatically and continuously (see section 6.4.3 on page 89). Save as User Program To store the selected parameters as a User Program, see section 6.
Advanced Operations Reading mode: 1. To highlight the required mode, start by pressing Reading mode. 2. Select the required mode, then press OK, then Return to return to the result display. 6.4.2 Take single wavelength measurements (single reading) 1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero. Note: The Read key is only active after the zero measurement has been completed. 2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read. 3.
Advanced Operations 6.5 Multi-Wavelength mode – measurements at more than one wavelength In the multi-wavelength mode, absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums, differences and relationships. Absorbance measures the amount of light absorbed by the sample, in units of absorbance. % Transmittance measures the percent of the original light that passes through the sample and reaches the detector.
Advanced Operations Table 13 Multi-wavelength setup options (continued) Options Description Recall Data Call up saved measurement data, wavelength scans or time courses, see section 5.3 on page 35. Instrument Setup Basic data of the instrument, see section 5.2 on page 23. λ / Absorbance formula: 1. Press Absorbance Formula. 2. The formula selected in the top key determines the number of wavelength and coefficent keys that will appear below.
Advanced Operations 3. To change a wavelength, press one of the ”λx:“ keys. Enter the desired wavelength coefficient into the numeric keypad. Press OK to confirm. 4. To change a coefficient, press one of the ”KX:“ keys. Enter the desired coefficient into the numeric keypad. Press OK to confirm. Note: The instrument allows entry of up to 5 significant digits, with a maximum of 4 significant digits after the decimal point. Concentration factor: 1. Press Concentration Factor: Off in the Options menu.
Advanced Operations 6.5.2 Complete a measurement in the multi wavelength mode 1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero. Note: The Read key does not become active until the zero measurement has been completed. 2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read. Note: The UV lamp is not switched on until a wavelength in the UV spectrum has been entered and the blank reading has been started.
Advanced Operations 6.6 Wavelength Scan mode – recording of absorbance and transmittance spectrums In the wavelength scan mode, the absorbance of the light in a solution over a defined wavelength spectrum is measured. The measurement results can be displayed as a curve, as percent transmittance (%T) or as absorbance (Abs). The collected data can be printed as a table or a curve. The data are available for formatting changes. These include automatic scaling and zoom functions.
Advanced Operations Table 14 Wavelength scan setup options (continued) Option Description Scale & Units Scale: In the automatic scaling mode, the y-axis is automatically adjusted so that the total scan is displayed. The manual scaling mode allows sections of the scan to be displayed. Units: Choice of absorbance or transmittance. Recall Data Call up saved measurement data, wavelength scans or time courses, see section 5.3 on page 35. Instrument Setup Basic data of the instrument, see section 5.
Advanced Operations 5. According to the selected wavelength range, select the interval from the actively displayed wavelength steps. In summery maximally 780 measuring steps can be accomplished during a Scan. Note: The maximum wavelength adjusts automatically if the difference between the maximum and minimum wavelength is not a multiple of the interval. 6. Press OK to return to the scan mode. Selected parameters are displayed along the graph‘s x-axis. Select view (displaying table) 1.
Advanced Operations 4. Press Return to return to the scan mode. Note: The Integral is shown instead of the date on the display. Note: For the next scan measurement the setting for the Integral will be On. Scale & units 1. Press Scale & Units. 2. Highlight the required units (Abs or %T). 3. Highlight Auto or Manual scaling on the graph´s y-axis. Note: If manual scaling is selected, use the alphanumeric keypad to set the limits ymin. and ymax..
Advanced Operations 3. Press Zero. ”Zeroing....“ appears below the graph as the baseline scan begins. 4. Insert the prepared sample cuvette/cell into the cuvette/sample cell holder. 5. Press Read. Below the graph ”Reading...“ appears and a graph of the absorbance or transmittance values at the scanned wavelengths is displayed continuously.
Advanced Operations 6.6.3 Work with reference scans There are two options to work with Reference Scan: Option 1: 1. Press Reference: Off in the Options menu to select another scan to display on the same screen with the current scan. Highlight the required scan number and press Highlight Reference. Note: After selecting a reference scan the Reference: Off key in the Options menu turns into Reference: On. Note: Only scans that have the same wavelength range and step can be displayed using the overlay option.
Advanced Operations Option 2: 1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero. 2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read. • The newly plotted wavelength scan curves are shown in black. • The absorbance or transmittance value and the associated wavelength are highlighted in black. 3. Press Options and then Reference: Off in the Options menu to select another scan to display on the same screen with the current scan.
Advanced Operations 6.7 Time course of absorbance/transmittance The Time Course Mode is used to collect data in either absorbance or transmittance for a user-specified length of time. After the data are collected, they can be displayed in either graphic or tabular format. 6.7.1 Time course setup parameters Press Time Course mode in the Main Menu. Press Options to configure parameters.
Advanced Operations Time & interval: 1. Press Time & Interval in the Options menu. 2. Input the total time and the reading time and press OK to confirm. Note: In total 500 measuring steps are possible. To select a total time and a time interval that would cause this number of measurements to be exceeded, the time interval is defined automatically and the OK key is inactivated. Scale & units: 1. Press Scale & Units in the Options menu. 2. Highlight Abs or %T as the required units. 3.
Advanced Operations • Select Mark to mark the next data point collected. This mark is not used by the instrument, but is available for the user and may indicate a significant event, such as the addition of a sample or other reagent. The mark is also shown in the table. • Select Stop to stop taking sample readings. 6.7.
Advanced Operations 6.7.3.1 Navigation of a time scan or a time scan analysis After a time scan has been completed, the time and the absorbance/transmittance data are displayed as a curve. Where the cursor is positioned on the curve, the elapsed time up to this point and the corresponding absorbance are highlighted. Table 17 Navigating the time scan Cursor Function/ Zoom Function Description Delta mode: A second cursor is highlighted.
Advanced Operations 6.8 System checks 1. Press System Checks in the Main Menu. The System Checks menu contains instrument information and various performance tests. 6.8.1 Instrument information 1. Press Instrument Information in the System Checks menu. 2. The model, serial number and software version are displayed.
Advanced Operations 6.8.2 Upgrade of the instrument software To obtain the software for the update from the Internet at www.hach.com: 1. Go to http://www.hach.com. 2. On the DR 5000 product page, click Lab System Software/Software Update Downloads under the heading Downloads. 3. Locate the appropriate download and follow the prompts for saving the file(s) to the USB memory stick. 4. On the DR 5000, press Instrument Update in the System Checks menu. 5.
Advanced Operations 6.8.3.1 Wavelength check The Wavelength Check test is used to check wavelength accuracy at 656.1 and 486.0 nm and to check bandwidth at 656.1 nm, only. 1. Press Wavelength Check in the Optical Checks menu. 2. Press λ to select the wavelength. 3. Make a choice and confirm with OK. 4. Press Start. Note: During the warm-up phase of the UV lamp, the message ”Lamp Warmup...“ is displayed.
Advanced Operations 5. The result is displayed. 6. Press Cancel to return to Optical Checks. 6.8.3.2 Noise check The Noise Check test is used to test the photometric noise in the instrument. This test can be used to test noise at any wavelength and at an absorbance level determined by a specific sample. 1. Press Noise Check in the Optical Checks menu. 2. Press λ to input the wavelength. 3. Input the wavelength and press OK to confirm. 4. Press Zero.
Advanced Operations 5. Insert sample cuvette/cell into the cell compartment. Close the cell compartment. 6. Press Start. 7. The result will be displayed. Thirty readings are averaged for the blank. The ”Average“ and ”Std Deviation“ are calculated from 100 consecutive absorbance readings (Noise, Sample, Reference). Note: Failings and passings will be defined by the user. 8. Press PC & Printer icon to send data to a PC or Printer. 9. Press Cancel to return to Optical Checks.
Advanced Operations 6.8.3.3 Stray light check The Stray Light test is used to measure the stray light in the instrument at 220.0 nm and 340.0 nm. 1. Press Stray Light Check in the Optical Checks menu. 2. Press λ to select the wavelength. 3. Make a choice and confirm with OK. 4. Press Zero. 5. Insert the sample cuvette/cell or reference filter into the cell compartment. Close the cell compartment. Press Start.
Advanced Operations 6. The ”Average“ and ”Std Deviation“ are calculated from 100 successive absorbance measurements. The result is displayed and is to be compared with the nominal/standard data (given in the quality control certificate) of the sample cell/test filter. Note: Failings and passings will be defined by the user. 7. Press Cancel to return to Optical Checks. 6.8.3.4 Absorbance check The Absorbance Check test is used to test the photometric accuracy and repeatability of the instrument.
Advanced Operations 7. The result is displayed and is to be compared with the nominal/standard data of the test filter. If a specific sample is used, the measured results must be evaluated against user-defined requirements. 8. Press Cancel to return to Optical Checks. 6.8.3.5 Drift check The Drift Check test is used to test the stability of the instrument. Note: The Drift Check runs 1 hour. 1. Press Drift Check in the Optical Checks menu. 2. Press λ to input the wavelength. 3.
Advanced Operations 5. The Drift Check runs 1 hour. This test takes a reading every minute for one hour. Every 15 minutes, linear regression is used to calculate the slope (rate of change) for the previous 15 minute interval. 6. The last reading and slope for each 15 minute interval are displayed. At the end of the hour, the overall values are calculated and displayed. The test proceeds to completion. 7. Press Cancel to return to Optical Checks. 6.8.3.
Advanced Operations 3. Press Edit. An automatic menu guidance queries values (filters, wavelength, nominal values and tolerances) given in the quality control certificate, to the following specifications: • Stray Light • Photometrical accuracy • Wavelength accuracy 4. Press OK when all values are entered and the overview is displayed. 5. Press Verification. 6. Insert the Multi Cell Holder in the cell compartment in such a way that the cell opening for 10 mm square cuvettes/cells is at the front.
Advanced Operations 8. Insert the different filter in the given order one after the other with the filter glass facing the front of the instrument. Press Next after inserting a filter and closing the cell compartment After the last measurement the results are displayed. 9. Press PC & Printer icon to send the data to a USB memory stick, PC or to a printer. The files will be stored automatically as CSV file (Comma Separated Value). The file name will be formatted as “Verification.csv“. 6.8.
Advanced Operations 6.8.6 Factory service The Factory Service menu is password protected. This menu is not intended for customer use. 6.8.7 Service time In order to ensure a regular inspection, an automatic memory reference for the service times can be entered. After switching the instrument on this memory reference will be activated and indicated at the appropriate time. 1. Press Service Time in the System Checks menu. 2. Select On and then Last Service to enter the date of the last inspection. 3.
Advanced Operations If the next service is due, the message "Next service is due!" is displayed after switching on the instrument. 6. Press OK to return to the Main Menu. Contact the manufacturer or distributor to arrange an appointment for the next service. 6.8.8 Instrument Backup Before the next service date the Instrument Backup menu offers the possibility to store all programs, measuring data, Operator ID, Sample ID, passwords and all adjustable data on a USB stick. 1.
Advanced Operations Note: If the Backup was already stored before, the message "Data already exists. Overwrite?" is displayed. Press OK to overwrite the data. If the file was stored the message Instrument Backup is stored to USB stick will be displayed. 4. Press OK to return to the System Checks menu. Restore backup data: Important Note: All current data will be overwritten when restoring the Backup file! 1. Press Instrument Backup in the System Checks menu. 2.
Advanced Operations Note: If the USB stick is not connected, the message "Please insert USB Memory" is displayed. Connect a USB stick, in order to store the data. Press OK to confirm and press Restore again. 4. Press OK to confirm after the message “Instrument Backup from S/N XXXXXXX. Restore?“ is displayed. 5. After the backup start the instrument again.
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Section 7 Maintenance CAUTION Potential Chemical, Biological Eye and Skin Hazards. Only qualified personnel should conduct the tasks described in this section of the manual. Important Note: Remove any cuvettes/cells that are still in the instrument and dispose of them or their contents using an approved disposal method. 7.1 Cleaning requirements CAUTION Potential Pinch, Eye, Burn and Chemical Hazards. Always disconnect power from the instrument before attempting any cleaning operations.
Maintenance 7.2 Lamp replacement CAUTION The UV lamp generates UV light. Do not look directly at an operating lamp without wearing UV protective eye glasses. The Lamp compartment is on the left side behind the display and is provided with ventilation on the back side. The tungsten and deuterium (UV) lamp are installed in the lamp compartment. On the back side a fan is installed for cooling of electric components. The ventilation system operates automatically. Refer to section 7.2.1 on page 122, section 7.
Maintenance 12. Switch the instrument on. 13. Reset the Lamp History, see section 6.8.5 on page 115. 7.2.2 Deuterium lamp (UV) replacement 1. Switch the instrument off. 2. Unplug the power cord. DANGER The instrument must remain switched off until the change of lamp has been completed. WARNING Burn Hazard. Wait until the lamp cools down. Contact with the hot lamp can cause burns. 3. Use a screwdriver to remove the cover from the back of the instrument (the screws may be slotted or cross-headed). 4.
Maintenance Figure 8 Lamp compartment 1 Tungsten lamp 5 Plug contacts for fan 2 Spring 6 Plug contact for deuterium lamp 3 Screws 7 Plug contact for tungsten lamp (with grip and cable) 4 Deuterium lamp (lamp socket) 7.3 Fuse replacement DANGER Certain electrical circuits within this equipment are protected by fuses against over-current conditions. For continued protection against a risk of fire, replace fuses only with the same type and rating specified.
Maintenance 3. Pull out the plastic base over the power cable socket. 4. Remove the defective fuse, see Figure 1 on page 14. 5. Insert new fuse (T 2 A H; 250 V) 6. Replace the plastic base. 7. Plug in the power cord. 8. Switch the instrument on. 7.4 Filter pad maintenance To determine when the filter mat needs to be replaced, inspect the filter mat every 3–6 months (in a relatively dust-free environment, this interval can be longer). 1.
Maintenance Figure 9 Base of DR 5000 with filter grid 1 Filter grid 2 Filter pad 3 Phillips screw Figure 10 Change of filter pad 1 126 Filter pad
Section 8 Troubleshooting Problem/Display screen Likely Cause Action Absorbance > 3.5! The measured absorbance exceeds 3.5 Dilute the sample and repeat the measurement Concentration too high! Calculated concentration is higher than 999999 Dilute the sample and repeat the measurement Error Barcode controlnumber? Please update program data! Deviation to the stored data Data updating Error Program not available.
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Section 9 Replacement Parts Description Cat.-No.
Replacement Parts 130
Section 10 Contact Information HACH Company World Headquarters P.O. Box 389 Loveland, Colorado 80539-0389 U.S.A. Tel (800) 227-HACH (800) -227-4224 (U.S.A. only) Fax (970) 669-2932 orders@hach.com www.hach.com Repair Service in the United States: HACH Company Ames Service 100 Dayton Avenue Ames, Iowa 50010 Tel (800) 227-4224 (U.S.A. only) Fax (515) 232-3835 Repair Service in Canada: Hach Sales & Service Canada Ltd.
Contact Information 132
Section 11 Limited Warranty Hach Company warrants its products to the original purchaser against any defects that are due to faulty material or workmanship for a period of one year from date of shipment unless otherwise noted in the product manual. In the event that a defect is discovered during the warranty period, Hach Company agrees that, at its option, it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges.
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Index A M Absorbance Check ................................................. 111 Absorbance Formula ......................................... 90, 91 Alphanumeric keypad .............................................. 21 Audio signals ........................................................... 25 Main Menu ............................................................... 22 Maintenance .......................................................... 121 Mark ........................................................
T Time & Interval ............................................... 101, 102 Time Course ..........22, 38, 39, 40, 41, 101, 103, 104 Time Course Scan Reading ................................... 102 Timer .................................. 25, 43, 53, 54, 56, 87, 90 Touch Screen .......................................................... 21 U Unpack the instrument ............................................. 13 Update .......................................................54, 58, 106 User Programs ..........
