DOC022.52.
Table of Contents Specifications ..................................................................................................................................................................................5 General information .....................................................................................................................................................................6 Safety information ...........................................................................................
Table of Contents Prepare the sample ..........................................................................................................................................................................21 Prepare a representative sample ..............................................................................................................................................21 Remove air bubbles from the sample ..........................................................................................
Table of Contents Prepare formazin standards .....................................................................................................................................................29 Calibration notes .......................................................................................................................................................................30 Formazin calibration procedure ................................................................................................
Table of Contents 4
Specifications Specification Details Specifications are subject to change without notice.
Specification Details Weight 3.8 kg (8.5 lb) Certification CE, cETLus 1 2 Turbidity specifications identified using recently prepared formazin standard and matched 1-inch sample cells. Intermittent electromagnetic radiation of 3 volts/meter or greater may cause slight accuracy shifts. Use of hazard information DANGER Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or serious injury.
Certification Canadian Radio Interference-Causing Equipment Regulation, IECS-003, Class A: Supporting test records reside with the manufacturer. This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations. Cet appareil numèrique de la classe A respecte toutes les exigences du Rëglement sur le matériel brouilleur du Canada. FCC Part 15, Class "A" Limits Supporting test records reside with the manufacturer. The device complies with Part 15 of the FCC Rules.
Figure 3 Instrument components Figure 2 Back overview 1 Power cord connector 4 DB9 connector for RS232 cable 2 Fuse holder 5 Air purge fitting 3 Power switch Product components Refer to Figure 3 to make sure that all components have been received. If any of these items are missing or damaged, contact the manufacturer or a sales representative immediately.
User interface Table 1 Key descriptions (continued) Key Figure 4 Keypad Description Turns signal averaging on or off. Turns on Setup mode and starts the selection of the setup number. Sends the data that is on the display to a printer or computer. Sends a calibration data report to a printer or computer when in Calibration mode. Sends diagnostic results to a printer or computer if held down when the instrument is turned on. Provides a print of the setup commands when in Setup mode.
Table 2 Light descriptions (continued) Figure 5 Indicator lights Light Description Manual Illuminated when the instrument is in manual ranging mode. Range Auto Illuminated when the instrument is in auto ranging mode. Range CAL? Turns on during a calibration if the calibration data is not within the acceptable range. Flashes when the instrument should be calibrated. Note: The CAL? light applies when a 25-mm sample cell is used.
4. Connect the power cord to a power socket with ground contact. 5. Push the power switch on the back of the instrument to turn the instrument on. Turn the keypad sound off (optional) By default, the instrument makes an audible sound when a key is pushed. To turn the keypad sound off: 1. 2. 3. 4. Push SETUP. The SETUP light turns on. Use the arrow keys to select 00. Push ENTER. Use the arrow keys to select the sound option: Option Description BP An audible sound is made when a key is pushed.
• All nephelometric (turbidity units of measure) calibrations are done at the same time. • The 4000-NTU standard does not have to be measured during calibration if FNUs will be measured. Push CAL after the 1000 NTU standard is measured to complete the calibration procedure. • The FNU values of StablCal standards and formazin standards are calculated using the conversion factors of 1 FNU = 1 NTU. StablCal calibration procedure 1. Push CAL. The S0 light turns on.
7. Remove the vial from the sample cell holder. 8. Do steps 5–10 for the other StablCal vials (from lowest to highest NTU standard). The S0 light turns on after the last vial is measured. 9. Push CAL. The instrument saves the new calibration data and goes back to Measurement mode. StablCal standards storage • Do not move a StablCal standard to a different container for storage. Keep StablCal standards in the plastic case provided with the cover closed. • Store at 5 to 25 °C (41 to 77 °F).
Measure the Gelex stray light standard Measure the Gelex stray light standard when the instrument is first received. Record the value on the Gelex vial with a permanent marker one time. 1. Clean the stray light standard with a soft, lint-free cloth to remove water spots and fingerprints. 2. Apply a small bead of silicone oil from the top to the bottom of the vial. 3. Use the oiling cloth to apply the oil equally to the surface of the vial. Remove the excess oil. Make sure that the vial is almost dry. 7.
Measure the Gelex secondary turbidity standards Measure the Gelex secondary turbidity standards each time the instrument is calibrated and record the new values on the Gelex vials with a water soluble marker. 1. Clean the Gelex vials with a soft, lint-free cloth to remove water spots and fingerprints. 2. Apply a small bead of silicone oil from the top to the bottom of the vial. 3. Use the oiling cloth to apply the oil equally to the surface of the vial. Remove the excess oil.
NOTICE Calibration verification At intervals, measure the Gelex secondary turbidity standard that is closest in value to the turbidity range to be measured. Do the steps in Measure the Gelex secondary turbidity standards on page 15, but do not change the value that is recorded on the vial. If the measured value is within ±5% of the value recorded on the Gelex vial, calibration is verified. If not, calibrate the instrument.
Indexing a single sample cell When measuring very low turbidity samples, use a single indexed sample cell or a flow cell for all measurements to get precise and repeatable measurements. As an alternative, optically matched sample cells can be used. Refer to Matching sample cells on page 19. Matched sample cells do not provide as good of accuracy and precision as a single indexed sample cell that is used for every measurement or a flow cell. 1.
7. Repeat step 6 until the lowest value is shown on the display. 18 English 8. Put an orientation mark on the marking band near the top of the sample cell where the lowest value is shown.
Matching sample cells To decrease the effects that optical differences among sample cells can have on turbidity measurements, measure samples in matched sample cells. It may not be possible to match all sample cells due to the differences in glass. 1. Rinse two or more clean, empty sample cells two times with dilution water and drain to waste. Fill the sample cells to the line (about 30 mL) with filtered dilution water and immediately put the cap on the sample cell.
7. Repeat step 6 until the lowest value is shown on the display. 8. Record the value. Put an orientation mark on the marking band near the top of the sample cell. 9. Put the second sample cell in the sample cell holder. Close the cover. Record the value when stable. 10. Remove the sample cell, turn it about 1/8 of a turn and put it in the sample cell holder again. Close the cover. 11. Repeat step 10 until the value matches the first sample cell value within ±0.005 FNU. 12.
Prepare the sample Proper sampling techniques are important to get accurate measurements. Prepare a representative sample A representative sample accurately reflects the true condition of the water source from which the sample was taken. To prepare a representative sample: • Gently but fully mix every sample before collecting aliquots (sample portions). Mix by gentle inversion only. Do not shake.
If possible, do not use heat to accelerate degassing. Heat may change the properties of the suspended particles and cause volatile components to come out of the solution. Gentle heat may be used to remove bubbles from very viscous samples when used with vacuum or ultrasound. If applying heat to the sample is necessary, do so only as much as is necessary to complete degassing. Before measurement, decrease the temperature of the sample to the initial temperature, then gently invert the sample.
Figure 6 Prepare filtered sample using membrane or glass-fiber filter Measurement notes Proper measurement techniques are important in minimizing the effects of instrument variation, stray light and air bubbles. For accurate and repeatable measurements: Instrument • Make sure that the instrument is on a level, stationary surface that is free of vibration during the measurement. • Instrument stabilization is immediate. No warm-up time is necessary.
• Avoid instrument operation in direct sunlight. Turbidity measurement procedure 1. Rinse a clean, empty sample cell two times with the solution to be measured and drain to waste. Fill to the line (about 30 mL) with sample and immediately put the cap on the sample cell. 7. Read and record the value when stable. Note: To send (via RS232) a measurement record, push PRINT. 24 English 2. Clean the sample cells with a soft, lint-free cloth to remove water spots and fingerprints. 3.
Measurement techniques Measurements may be made with different operation mode settings and optional accessories. Calibrate the instrument whenever the sample cell pathlength is changed. Manual or automatic ranging The manufacturer recommends that ranging be set to automatic for most measurements. The setting can be changed at any time during sample measurement. Push RANGE repeatedly to step the instrument from automatic ranging to manual ranging and then scroll through the manual range settings.
Note: Air bubbles collect in areas that are not cleaned fully. Figure 8 Standard shop air 3. Clean the exterior surface of the flow cell with a soft, lint-free cloth to remove water spots and fingerprints. 4. Apply a small bead of silicone oil from the top to the bottom of the flow cell. Note: Use only the provided silicone oil. This silicone oil has the same refractive index as the flow cell glass and masks minor glass scratches. 5.
6. Air dry the parts after cleaning. Flow cell maintenance • Keep all parts of the flow cell assembly clean. • At intervals, replace all the tubing to make sure that the system is clean. Keep the tubing as short as possible to minimize air locking and lag time of sample flow. Locate the instrument as close to the drain as possible. Flow cell operation • Do not use the flow cell for samples that contain large particles that may collect and stop the sample from flowing.
Install a cell adapter Connect to a printer or computer 1. Align the tab on the cell adapter toward the front of the instrument (Figure 9). 2. Put the cell adapter in the sample cell holder. 3. Calibrate the instrument each time the sample cell diameter is changed. Calibrate using sample cells of the same path length as the sample cell that will be used to measure samples.
3. Push ENTER. Table 4 RS232 command set (continued) 4. Use the arrow keys to change the value. 5. Push ENTER. 6. Push SETUP. Computer (RS232) commands A communication program (i.e., such as Window Terminal or ProComm Plus) is recommended for computer operation of the instrument. Configure the communication program to the RS232 connection settings. Refer to Configure the RS232 connection on page 28. Table 4 shows the RS232 command set for the instrument.
Table 5 Formazin standard preparation Standard Step 1 Step 2 Step 3 20 NTU Add 100 mL of dilution water to a clean 200-mL Class A volumetric flask. Refer to Prepare dilution water on page 20. With a TenSette® Pipet, add 1.00 mL of well-mixed 4000-NTU formazin stock solution to the 200mL flask. Dilute to the mark with dilution water. Stopper and mix. 200 NTU Add 50 mL of dilution water to a clean 100mL class A volumetric flask. With a TenSette1 Pipet, add 5.
Formazin calibration procedure For the best accuracy, use four matched sample cells or the same sample cell for all measurements during calibration. Refer to Matching sample cells on page 19. 1. Push CAL. The S0 light turns on. The NTU value of the dilution water used in the last calibration is shown. 2. Rinse a clean sample cell two times with dilution water. Fill the sample cell to the line (about 30 mL) with dilution water and immediately put the cap on the sample cell.
7. Push ENTER. The instrument display counts down from 60 to 0, and then measures the standard. 8. Remove the sample cell from the sample cell holder. The instrument shows the next expected standard (e.g., 20.00). The S1 light turns on. 9. Do steps 5–11 for the other formazin standards (from lowest to highest NTU standard). Mix each formazin standard well and rinse the sample cell two times with formazin standard before the sample cell is filled. 10. Push CAL.
• The calibration points that are shown on the display must be changed as they occur so they agree with the turbidity of the user-defined standards. Refer to Change the calibration points on page 33. 5. Do steps 2–4 again if necessary to change the other digit. 6. Push ENTER to save the change and start the measurement. Note: Unknown performance may occur if standards other than the recommended calibration points are used. The recommended calibration points (< 0.
To replace a fuse, refer to the illustrated steps in Figure 10. Note: Any calibration being calculated when an error occurs, is discarded. The old calibration is kept. Figure 10 Replace a fuse Table 6 Error codes Error Description ERR01 The turbidity of the dilution water is greater than 0.5 NTU. ERR02 Troubleshooting Refer to the tables in this section for error codes, diagnostic codes, common problem messages or symptoms, possible causes and corrective actions.
Table 6 Error codes (continued) Error Description ERR06 A/D is under the range ERR07 Light leak ERR09 Printer time out error ERR10 System voltage out of range ERR11 System loop test error Note: To print a diagnostic report, hold down PRINT, then turn the instrument on. Table 7 Diagnostic codes Solution 1. Make sure that no object is in the light path. 2. Contact Customer Service if necessary. 1. Make sure that the cover for the sample cell compartment is closed. 2.
Replacement parts and accessories Accessories Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to the company website for contact information. Description Replacement parts Calibration kit, StablCal®, 500 mL each Quantity Item no.
Replacement parts and accessories (continued) Description Quantity Item no. 0.1 NTU, StablCal™ low-level turbidity verification standards (not for instrument calibration) 100 mL 2723342 0.3 NTU, StablCal™ low-level turbidity verification standards (not for instrument calibration) 100 mL 2697942 0.5 NTU, StablCal™ low-level turbidity verification standards (not for instrument calibration) 100 mL 2698042 TenSette® Pipet, 1.0-10.
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HACH COMPANY World Headquarters P.O. Box 389, Loveland, CO 80539-0389 U.S.A. Tel. (970) 669-3050 (800) 227-4224 (U.S.A. only) Fax (970) 669-2932 orders@hach.com www.hach.com © HACH LANGE GMBH Willstätterstraße 11 D-40549 Düsseldorf, Germany Tel. +49 (0) 2 11 52 88-320 Fax +49 (0) 2 11 52 88-210 info@hach-lange.de www.hach-lange.de HACH LANGE Sàrl 6, route de Compois 1222 Vésenaz SWITZERLAND Tel. +41 22 594 6400 Fax +41 22 594 6499 Hach Company/Hach Lange GmbH, 2012. All rights reserved.
