Quad-Z 215 Liquid Handler User's Guide LT2545/©2003 Gilson, Inc.
Table of Contents Declaration of Conformity 1 Introduction Safety Precautions ............................................................... 1-1 Description ............................................................................ 1-2 Unpacking ............................................................................. 1-3 Standard Equipment .................................................... 1-4 Accessories ..................................................................... 1-4 Customer Service ..
3 Operation Front Panel ............................................................................ 3-2 Start Button .................................................................... 3-2 Stop Button ..................................................................... 3-2 LED Display ................................................................... 3-2 Power Indicator Light .................................................. 3-2 Start Up .................................................................
Troubleshooting Error Messages ..................................................................... 5-2 Mechanical ............................................................................ 5-5 Electrical ................................................................................ 5-6 Liquid Level Detector .......................................................... 5-8 Repair and Return Policies ................................................
Declaration of Conformity Application of Council Directives: 89/336/EEC, 73/23/EEC Standards to which Conformity is Declared: EN61326, EN61000-3-3, EN61000-3-2, EN61010-1 Manufacturer’s Name ........................................... Gilson, Inc. Manufacturer’s Address ....................................... 3000 W. Beltline Highway Middleton, WI 53562 EC Office Address .................................................. Gilson S.A.S. 19 Avenue des Entrepreneurs, B.P.
Introduction 1 Safety Precautions For safe and correct use of this instrument, it is recommended that both operating and service personnel follow the instructions contained in this guide when installing, cleaning, and maintaining this instrument. Because the probes installed on the Z-arm may contain a dangerous substance, use the safety shield included with the instrument and do not interfere in the work area of the instrument until the liquid handler has completed its procedures.
1 Description Introduction Description The Gilson Quad-Z 215 Liquid Handler is an XYZ robot that can automate any number of manual liquid handling procedures. The Quad-Z has four independently operated probes with variable horizontal spacing (from 9 to 18 mm) allowing access to virtually any tube, vial, or microplate configuration. The optional 849 Multiple Injection Module can be configured with four sample loops of varying capacities to provide complete flexibility for open access laboratories.
Introduction 1 Unpacking Unpacking The Quad-Z 215 Liquid Handler is delivered with all major components already assembled except for auxiliary parts such as the Z-arm, probe, racks, tubing, etc. Keep the original container and packing assembly in case the liquid handler must be returned to the factory.
1 Unpacking Introduction Standard Equipment Once the liquid handler and the accessories containers have been unpacked, you should have the following: Quad-Z 215 Liquid Handler 444 QuadDilutor with accessories Locator plate with one drain base (includes four mounting screws) Rinse drain package which includes: • 2-liter waste bottle • Cap with quick connect fitting • Rinse station with fittings • 5 feet of Tygon waste tubing with quick connect fitting Z-arm and control cable with retaining clip and level
Introduction 1 Customer Service Customer Service Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following assistance: sales, technical applications, and instrument repair. If you need assistance, please contact your Gilson representative or if you are in the United States call the Gilson Customer Service Department at 800-4457661 or 608-836-1551. You can also contact the Customer Service Department via its e-mail address: service@gilson.com.
1 Technical Specifications Introduction Technical Specifications Please be aware of the following before operating the liquid handler. Warning: Changes or modifications to the liquid handler not expressly approved by Gilson could void the factory-authorized warranty. The liquid handler has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC commercial environment.
1 Introduction Technical Specifications 1-7
Technical Specifications Introduction 1-8 1
Installation 2 This section takes you through the steps for setting up your Quad-Z 215 Liquid Handler.
2 Locator Plate Installation Installation Locator Plate Installation The locator plate serves two functions: • Positions the racks and accessories that fit onto the bed of the liquid handler. • Contains liquid spills, such as those caused by overflowing vessels. The locator plate and its four mounting screws are shipped in a separate box with the liquid handler’s accessories.
Installation 2 Armlock Removal Armlock Removal The armlock on the liquid handler secures the Y-arm during shipment. You must remove the armlock prior to installing the Z-arm and operating the instrument. If the armlock is not removed, the liquid handler cannot move in the X-direction. This results in an error state during operation. If you need to move the liquid handler, always reinstall the armlock. This safeguards against mechanical damage.
2 Z-Arm Installation Installation Z-Arm Installation Follow these steps to install the Z-arm: 1 Using a Phillips screwdriver, loosen the mounting screw on the Z-arm mounting bracket located on the Y-arm. Turn counterclockwise to loosen. 2 Partially pull out the bracket. Do not remove completely. 3 Place the Z-arm into the mounting bracket. You will need to insert one side of the Z-arm into place at a time. 4 Tighten the screw on the mounting bracket until the Z-arm is secure.
Installation 2 Z-Arm Installation Installing the Z-Arm Cable Support Rod 1 Using the two Phillips screws, attach the cable support rod bracket in the holes located in the rear of the X-arm extrusion. 2 Extend the arm to the extreme X- and Y-direction to ensure that the cable will have enough slack. Plug the Z-arm control cable into the back topside of the control cabinet. The control cable should be tucked into the groove located in the top of the X-arm extrusion.
2 Probe Installation Installation Probe Installation There are different probes available for use on the Quad-Z 215 Liquid Handler. Depending upon your application, you have purchased the appropriate probes and probe guide inserts. When installing the probes or custom sized probe guide inserts (1.3 or 1.5 mm) refer to the following procedures and diagrams. Installing the Probe Guide Inserts Your Quad-Z is delivered with 1.5 mm probe guide inserts installed.
Installation 2 Plumbing Connections Plumbing Connections Transfer Tubing Installation 1 Connect one end of each piece of transfer tubing to the 444 QuadDilutor. Refer to the 444 QuadDilutor User’s Guide for more information. 2 Locate the level sensing cables (LLD cables) supplied with the Z-arm. 3 Pass the other end of each transfer tubing through the open end of an LLD cable casing nearest the right-angle connector. The tubing should exit the back of the LLD cable casing approximately 6.
2 Plumbing Connections Installation Installing the Level Sensing Cables To install the level sensing cable: 2-8 1 Plug the right-angle connector on one end of the cable into socket one on the back of the Z-arm. 2 Snap the cable into the LLD cable guide (refer to the picture above). 3 Loosen the small hexagonal nut on the probe holder for probe one. Attach the split-tongue connector to the nut. Retighten the nut until snug (approximately one turn after finger tightening).
Installation 2 Plumbing Connections Rinse Station and Drain Waste Tubing Installation You’ll clean the probe using the rinse station. To eliminate carryover of liquids, the rinsing procedure pumps an excess volume of diluent or probe washing solution through the probe and out into the rinse station. The small diameter of the rinse station inserts allow the outside of the probe to be washed along with the inside.
2 Plumbing Connections Installation Installing the rinse station The base of one rinse station is shipped already secured to the locator plate and is located at the rear of the locator plate. The locator plate can hold an optional second rinse station or you can move the rinse station base to the alternate location. Before installing the rinse station, make sure the locator plate has been properly installed with the previously-installed rinse station base located at the rear of the instrument bed.
Installation 2 Rack Setup Rack Setup The Quad-Z 215 Liquid Handler is equipped to locate Code 20-, 30-, 200-, and 500-series racks. See Appendix B for a list of racks available for the liquid handler. Depending on the racks you’re using, refer to the appropriate procedures on the following pages.
2 Rack Setup Installation Code 200-Series and Code 20-Series or Code 30-Series Racks To use a combination of Code 200-series and Code 20-series or Code 30-series racks on the locator plate: 2-12 1 Install each Code 200-series rack as described on page 2-11. 2 For each Code 20-series or Code 30-series rack, place an adapter plate onto the locator plate. Fit the plate so that the slots on the adapter plate align with the pins on the locator plate.
Installation 2 Rack Setup Only Code 20-Series or Code 30-Series Racks If all your racks are Code 20- or Code 30-series racks and you do not have the optional 849 Multiple Injection Module installed, follow the steps below. 1 2 Install the locator tray (part number 2504627, ordered separately) onto the locator plate of the liquid handler. • For Code 20-series racks, the handles face the front. • For Code 30-series racks, the hose fittings should face the back.
2 Final Z-Arm Adjustment Installation Final Z-Arm Adjustment Follow these steps to adjust the Z-arm to the proper height. 1 Turn off power to the liquid handler. 2 Locate the appropriate Z-height adjustment tool in the accessory package. Two Z-height adjustment tools are supplied in the package. Z-height adjustment tool 3 2-14 Part number Description 25051094 125 mm Z-height adjustment tool. Use this tool to adjust the Z-arm on the Quad-Z 215 Liquid Handler for liquid handling and injection.
Installation Loosen the mounting screw on the Z-arm mounting bracket and slightly raise the Z-arm. 5 Manually move the liquid handler’s arm so the Z-arm is centered over the Z-height adjustment tool. 6 While holding Z-height adjustment tool flat against the locator plate, use the other hand to lower the Z-arm until it lightly rests on the adjustment tool. 7 Tighten the mounting screw on the Z-arm mounting bracket so the Z-arm is secure.
2 Electrical Connections Installation Electrical Connections Rear Panel 1 2 3 4 5 6 7 8 9 2-16 Input/Output (I/O) ports Gilson Serial Input/Output Channel (GSIOC) port Keypad port (not used on the Quad-Z) RS-232 port Fuse drawer Power switch Power receptacle Unit ID selector Baud rate/mode selector
Installation 2 Electrical Connections Input/Output Ports You can use the input and output contacts found on the rear panel of the liquid handler to control peripheral devices. Refer to the diagram on page 2-16 for the location of the input/output ports. Contact inputs The input terminal block of the liquid handler has eight contacts. All of the inputs are paired, and each pair includes a GROUND reference ( ). The contact input pairs are labeled A, B, C, and D.
2 Electrical Connections Installation Making connections To prepare and make connections with the 2conductor cable: 1 Cut the cable into pieces of appropriate length. 2 Strip about 0.25 cm of insulation from each end of the cable. 3 Remove the terminal block connector from the liquid handler. Insert each wire into the appropriate slot on the terminal block connector. Note: When making connections, be sure to maintain the correct orientation of the connector relative to the port.
Installation 2 Electrical Connections RS-232 Port The RS-232 port is used to transfer information between the liquid handler and a computer. For the location of the RS-232 port, refer to the diagram on page 2-16. Be sure your computer is turned off before making any connections. To connect your computer to the liquid handler, you’ll need an RS-232 cable. Obtain a cable with D-connectors that are appropriate for the liquid handler and your computer. The liquid handler requires a 25-pin male D-connector.
2 Electrical Connections Installation GSIOC Port Gilson systems feature a two-way communication interface between the computer and most Gilson modules. Communication occurs along the Gilson Serial Input/Output Channel (GSIOC). The liquid handler can convert the RS-232 signal levels used by computers to the RS-422/485 signal levels required by the GSIOC and vice versa. (See page 2-19 for information on making the RS-232 connection between the liquid handler and computer.
Installation 2 Electrical Connections Unit ID and Baud Rate/Mode Selection Use the SW1 selector to choose a different unit ID and the SW2 to choose a different baud rate/mode. If necessary, refer to the diagram on page 2-16 for the location of these selectors. Unit ID The unit ID identifies the liquid handler to Gilson software packages that can issue GSIOC commands to the liquid handler. At the factory, Gilson set the unit ID to 22.
2 Electrical Connections Installation Baud rate/mode As a default, the baud rate/mode is set to 6, indicating that the liquid handler is set for a baud rate of 19200 and is a master device. You’ll need to change the selection to 0 (zero) if the liquid handler is connected via the GSIOC to a Gilson system and is being controlled by the 506C System Interface from Gilson control software. A setting of 0 indicates the liquid handler is a slave device and the baud rate is being clocked externally.
Installation 2 Electrical Connections Fuses You received the liquid handler without any fuses installed. To install the fuses: 1 Locate the accessory package containing the fuse drawer appropriate for your line voltage. Discard the other fuse drawer. 2 Locate the accessory package containing the 5.0A “T” Slo-Blo fuse (5 x 20 mm size) fuses. 3 Install the fuse(s) into the fuse drawer. The fuse drawer for 100/120V accepts one fuse. The fuse drawer for 220/240V accepts two fuses.
2 Electrical Connections Installation Power Cord Connection Locate the appropriate power cord for your line voltage. Discard the other power cord. Use the power cord to connect the liquid handler to an AC power source.
Operation 3 The Quad-Z 215 Liquid Handler is controlled as follows: • Via a method running on a personal computer. You create the method using 735 Sampler Software (ordered separately, part number 2106773532). If you have not already done so, install the 735 Sampler Software. Refer to the documentation supplied with the software.
3 Front Panel Operation Front Panel The front panel of the liquid handler contains a Start button, Stop button, LED display, and power indicator light. Start Button The Start button can be used to home the XYZ-arm when the liquid handler is first powered up or when the motors for the XYZ-arm have been relaxed. When pressed, the yellow LED lights.
Operation 3 Start Up Start Up To start the liquid handler: 1 Make sure the liquid handler is connected to a power source. 2 Turn on the liquid handler using the power switch located on the rear panel. Refer to rear panel diagram on page 2-16 if necessary. The power indicator light on the front panel illuminates. When power is turned on, the liquid handler beeps and displays the current version of its installed firmware.
3 Running Programs Operation Running Programs The liquid handler is controlled by programs executed from a personal computer. Executable (.EXE) programs can be run from a computer. The computer is connected to the liquid handler via an RS-232 cable. Refer to Section 2 for correct installation of the RS-232 cable between the liquid handler and the computer.
Operation 3 Configuring the Liquid Handler Configuring the Liquid Handler The liquid handler comes from the factory with its configuration set by Gilson. Configuration information is stored in the non-volatile memory of the liquid handler. Prior to using the liquid handler for the first time, it is important to review and adjust the default configuration to make sure it is correct for your application. The following pages describe how to use 215 Setup Utility to configure the liquid handler.
3 Configuring the Liquid Handler Operation About The About tab allows you to indicate the unit ID of the instrument being configured so the 215 Setup Utility can communicate with that instrument. This tab also lists software version and copyright information for the 215 Setup Utility. Unless the Non-Volatile RAM has been cleared, information about the type of 215 you are using will appear automatically. You will see the following indicating that you are using a Quad-Z 215 Liquid Handler: 215QADvX.XX.
Operation 3 Configuring the Liquid Handler Pump Type Because the Quad-Z 215 Liquid Handler uses an external dilutor, Blank Front is selected and all options are inactive. Syringe Options All options on this tab are inactive for the Quad-Z 215 Liquid Handler because it has an external dilutor.
3 Configuring the Liquid Handler Operation Rinse Site The instrument stores the location of a rinse site in memory. This allows the instrument to move to this location before homing the syringe and prevents the spilling of waste liquid or rinse diluent. You must modify the rinse site coordinates if you have a Quad-Z 215 Liquid Handler. Refer to the following table to select the correct X, Y, and Z coordinates depending on the kind of probe rinse that will be performed.
Operation 3 Configuring the Liquid Handler Liquid Detector The Liquid Detector tab allows you to adjust the default sensitivity for liquid detection. Note: Gilson control software (such as 735 Sampler Software) will override these settings. Raising the percentage suppresses false liquid detection while lowering the percentage increases sensitivity for situations where liquid is harder to detect.
3 Configuring the Liquid Handler Operation Adjust XY The Adjust XY tab allows you to test whether the instrument is properly adjusted and to make minor adjustments to the X- and Y-axis offsets if needed. You may need to use the options under this tab if the probe is not accessing the injection port of the injection module installed or the vessels in the installed racks. The X offset and Y offset text boxes display the current offsets stored in the instrument’s memory.
Operation 3 Configuring the Liquid Handler model 819: X-coordinate: 544.0 mm Y-coordinate: 3.8 mm If the model 819 is not installed next to the right support use the following coordinates: X-coordinate: 351.1 mm Y-coordinate: 1.3 mm model 841: X-coordinate: 475.3 mm Y-coordinate: 3.8 mm model 889: X-coordinate: 336.1 mm Y-coordinate: 3.8 mm model 849: X-coordinate: 336.1 mm Y-coordinate: 3.
3 Configuring the Liquid Handler Operation Z Height Use the Z Heights tab to identify the size of the installed Z-arm and the height at which the Z-arm is clamped. The tower height options are inactive for the Quad-Z 215 Liquid Handler because there is only one tower height available. The Z-arm can be clamped at an adjustable height over the locator plate. You can set this height so that the liquid handler is able to properly find heights that you specify. Type the clamp height in millimeters.
Operation 3 Configuring the Liquid Handler Home Phase Use the Home Phase tab to display the current Xand Y-phase of the instrument. Clicking Start causes the liquid handler to perform the phase procedure. This procedure consists of the liquid handler homing itself 10 times. The liquid handler finds out where home is located by “feeling” for the back and left walls of the unit. The liquid handler expects to find these walls in the same place each time. If it does not, you will get an error.
3 Configuring the Liquid Handler Operation Safety Contact The instrument has provisions for connecting safety devices that your application may require, as long as they present a contact closure or TTL type interface. The Safety Contact tab allows you to specify which input contact is connected to the safety device and what is the active state of that device. The function of the safety contact is equivalent to pressing the Stop button on the instrument’s front panel.
Operation 3 Configuring the Liquid Handler Sound and Display Use the options in the Sound and Display tab to adjust the brightness of the display, sound level, and tone. Clicking Beep tests the sound level and tone that are currently selected. For the L.E.D. brightness, you can select a range of 0 through 7 where 0 is the dimmest and 7 is the brightest. Default setting is 5.
3 Testing the Liquid Handler’s Contacts Operation Testing the Liquid Handler’s Contacts Following is a description of how to use the 215 Contact Test Utility to toggle output contacts to determine if the correct contact connections have been made to peripheral devices to be controlled by the Quad-Z 215 Liquid Handler. The program also identifies the state of input contacts and lets you test the Start and Stop buttons on the liquid handler.
Maintenance 4 To obtain optimum performance and maximum life from the Quad-Z 215 Liquid Handler, it is important to keep the instrument well-maintained. This section contains some general guidelines that will help you to maintain your liquid handler.
4 Helpful Hints Maintenance Helpful Hints In order to keep your liquid handler at peak performance, Gilson recommends that you do the following: 4-2 • Change or clean the tubing regularly to maintain maximum performance. • Flush the probe housings and rinse stations daily with distilled or deionized water. On a weekly basis, flush with a 10% solution of bleach or weak detergent. • Check periodically to ensure that all fittings are tight. • Wipe up all spills immediately.
Maintenance 4 Cleaning Cleaning Cleaning the Liquid Handler The liquid handler should be cleaned occasionally using a dry, clean cloth. Or, if necessary, use a cloth dipped in soapy water. If liquid is accidentally spilled on the liquid handler, wipe the instrument using a dry, clean cloth. Cleaning the Fluid Path Depending on your use of the liquid handler, it may be necessary to flush the entire fluid path.
4 Cleaning Maintenance Cleaning methods Depending on the samples or reagents that come into contact with the fluid path, you may need to vary your cleaning methods accordingly. Use the following cleaning protocols as references and make any changes to them as required for the samples and reagents being pumped for your application. Proteins and peptides - Follow this procedure if the fluid path is in contact with proteins and peptides: 4-4 1 Prime the fluid path using a weak detergent solution.
Maintenance 4 1 Prime the fluid path using a 0.1N NaOH solution. 2 Pause the priming sequence. 3 After 10 minutes, resume priming the fluid path using distilled or deionized water. Prime until the fluid path has been flushed with water. 4 Pause the priming sequence. 5 Prime the fluid path using a 0.1N NaOH solution. Continue to prime until the fluid path has been flushed with 0.1N NaOH. 6 Pause the priming sequence.
4 Maintenance Cleaning Biological fluids - Follow this procedure if the fluid path is in contact with biological fluids such as blood products: 4-6 1 Make a solution of 10% bleach by adding one part of commercial bleach to nine parts of water. 2 Prime the fluid path using the bleach solution until the entire fluid path has come into contact with bleach. 3 Pause the priming sequence.
Maintenance 4 Lubricating Lubricating Always switch the power to off when making adjustments to (such as lubricating) the liquid handler. The potential exists for bodily harm if you interfere with the work area of the instrument while it is running. Horizontal Pitch and Vertical Rods Refer to the instructions and diagrams on the next few pages for information on lubricating the horizontal pitch and vertical rods on the Quad-Z 215.
4 Lubricating Maintenance 6 Dispense another small bead of grease on the applicator. 7 Apply the grease to the visible and accessible areas on the vertical rods and horizontal pitch rods on the Z-arm (see below). 8 Repeatedly (and slowly), move the probe holders up and down to distribute the grease along the vertical rods.
Maintenance 4 Replacing Parts Replacing Parts Replacing Tubing It is important to keep all tubing clean and free of crimps. Tubing that has become dirty, blocked or crimped can result in poor accuracy and precision, or loss of air gap. Replace both the transfer tubing and inlet tubing as needed. See Appendix A for part numbers for replacement tubing. For tubing installation procedures, see Section 2.
4 Replacing Parts Maintenance Installing different type of probe To install a replacement probe of a different type than is currently installed, you may want to obtain a new probe guide insert for precise XY probe accuracy. 4-10 1 Remove the transfer tubing’s 1/4"-28 fitting connected to the top of the isolation probe holder. 2 Grasp the current probe and push it up through the top of the isolation probe holder. 3 Remove the current probe guide insert by turning it counterclockwise.
Maintenance 4 Replacing Parts Replacing a Level Sensing Cable Removing the level sensing cable 1 Detach the transfer tubing from the top of the isolation probe holder by using the supplied headless nut extender (part number 49041032) to loosen and remove the 1/4"-28 nut and ferrule. 2 Unplug the right-angle connector for the liquid level sensing cable (LLD cable) from the socket on the back of the Z-arm. 3 Detach the LLD cable from the LLD cable guide.
4 Replacing Parts Maintenance Installing the new level sensing cable 4-12 1 Locate the new level sensing cable supplied with the Z-arm. 2 Pass the transfer tubing through the open end of an LLD cable casing nearest the right-angle connector. The tubing should exit the back of the LLD cable casing approximately 6.5 cm from the end. 3 Connect the transfer tubing to the top of the isolation probe holder using a 1/4"-28 nut and ferrule supplied with the tubing.
4 Maintenance Replacing Parts Replacing a Fuse A blown fuse may indicate the existence of another problem in the instrument. If the replacement fuses blow, don’t try others. Contact your local representative or Gilson. See Before calling us on page 5-8. To change a fuse, follow these steps. 1 Disconnect the power cord from the power outlet and from the rear panel receptacle. 2 Locate the fuse drawer on the rear panel. See page 2-16 if necessary.
4 Checking Position Alignment Maintenance Checking Position Alignment The 215 Setup Utility (Adjust XY tab), described in Section 3, allows you to test whether the liquid handler is properly aligned and to make minor adjustments to the X-axis and Y-axis offsets if needed. You may need to use the options under this tab if the probe is not accessing the injection port of the injection module installed or the vessels in the installed racks. This tab is described below.
Maintenance 4 Transporting the Liquid Handler Transporting the Liquid Handler When moving the liquid handler to another location or when sending it back to the factory, do not use the Y-arm as a handle. Reinstall the armlock (see Section 2) and always lift the liquid handler from the base.
Troubleshooting 5 5-1
5 Error Messages Troubleshooting Error Messages Error Description Solution 15 • Send the buffered ~9 GSIOC command to reset the NV-RAM and initialize to the default value. Run 215SETUP.EXE or the 215 Setup Utility program • Replace the main board NV-RAM checksum is invalid 16 X scale factor is invalid Contact the Gilson Customer Service Department. 17 Y scale factor is invalid Contact the Gilson Customer Service Department.
5 Troubleshooting Z target more than maximum Z Send the immediate Q command using the GSIOC Utility Program to read the travel range. Correct the error in the program controlling the liquid handler. 30 X encoder inactive Contact the Gilson Customer Service Department 31 Y encoder inactive Contact the Gilson Customer Service Department 32 Z position sensor inactive Contact the Gilson Customer Service Department 33 Safety contact activated Release contact then restart.
5 Mechanical Troubleshooting Mechanical Probe(s) no longer finding tube center 5-4 • Probe(s) may be bent. Straighten or replace the probe. • Incorrect tray file defined. Review and if necessary change the tray file. • Liquid handler may be misaligned. Perform the position alignment procedures, described on page 4-14.
Troubleshooting Input functions not operating • Make sure connections into terminal block connector are secure. • Make sure terminal block connector is secure in input/output port. • Check connections for proper pin assignments. • Be sure pins from external devices are assigned correctly. • Check polarity of input. Inputs should be a contact closure. If not, it must be TTL level (logic 0 activates). • Confirm that source supplying input to liquid handler is working.
5 Electrical Troubleshooting Unit not operational • Make sure power is turned on. • Check AC power cord connections. • Try different AC outlet. • Check fuse(s); replace if necessary. • Check all liquid handler connections and make sure that the unit is plugged in. Unit blows fuses • 5-6 Contact the Gilson Customer Service Department.
Troubleshooting 5 Liquid Level Detector Liquid Level Detector Liquid level detector not detecting liquid level • Ensure that the level sensing cables are plugged in. • Check sensitivity setting in the 215 Setup Utility (see page 3-9) and lower the percentage. • Check if liquid is detectable. Liquid level detection works only if there is electrical conductivity in your liquid. Liquid level detecting will not work with most non-polar liquids.
5 Repair and Return Policies Troubleshooting Repair and Return Policies Before calling us Gilson Customer Service personnel will be able to serve you more efficiently if you have the following information: • • • • • • the serial number and model number of the equipment involved. The serial number is visible on the back of the control panel of the liquid handler.
Troubleshooting 5 Repair and Return Policies Rebuilt exchange For some units, rebuilt exchange components are available. Contact Gilson for details. Return procedure In the United States, contact the Gilson Customer Service Department to obtain authorization before returning any Gilson equipment. To return a piece of equipment: • Carefully pack the unit to prevent damage in transit. Check with Gilson regarding proper method of shipment.
Replacement Parts and Accessories A Probes 2507214 Non septum-piercing probe; constricted tip, capacitive level-sensing, stainless steel. Dimensions: 269 x 1.8 x 1.4 mm ID (tip dimensions: 1.5 x 1.2 x 0.8 mm ID). Requires probe guide insert (part number 25064473) for precise XY accuracy. 2507215 Non septum-piercing probe; constricted tip, capacitive level-sensing, stainless steel. Dimensions: 269 x 1.3 x 0.8 mm ID (constricted tip: 1.5 x 0.9 x 0.45 mm ID).
A Replacement Parts and Accessories Appendix 2507255 Non septum-piercing probe; beveled tip, capacitive level-sensing, stainless steel. Dimensions: 269 x 1.5 x 0.4 mm ID. Requires probe guide insert (part number 25064472) for precise XY accuracy. Control Software 2106773532 735 Sampler Software running directly from PC; requires PC with Microsoft Windows NT and serial cable. Probe Guide Inserts 25064473 Probe guide insert for 1.8 mm outer diameter probes. 25064472 Probe guide insert for 1.
A Appendix Waste bottle (2 liter) with lid and quick-connect fitting 470343706 Tygon tubing (5/16" ID x 7/16" OD) for connection between rinse station and waste bottle; per foot 23077332 Quick-connect fitting to connect Tygon tubing to waste bottle Rinse Station 25045525 Multiple Probe/Quad-Z 215 rinse station; connects to rinse station base 25245512 Rinse station base; attaches directly to 215 locator plate 23077333 Y-connector to connect two rinse stations to one waste bottle Rack Accessories
A Replacement Parts and Accessories Appendix Cables and I/O Accessories 25061401 36083121 36083122 36083123 638308512 638310512 709910206 36078143 6730504007 Level-sensing cable Serial cable, IBM PS/2-type, 25 to 25 pin Serial cable, IBM AT-type, 9-pin female to 25-pin male Serial cable adapter, 9-pin female to 25-pin male Terminal block connector, 8-pin Terminal block connector, 10-pin 2-conductor interconnect wire, 6', for making contact connections Shielded GSIOC cable, 30" 5.0A, T-5.
Racks B The Quad-Z 215 Liquid Handler can be configured with a variety of rack types and sizes. The following pages describe the racks that can be purchased for use on the liquid handler. Refer to Section 2 for rack installation procedures.
B Racks Appendix Code 20 rack For 108 vessels Material: polypropylene Vessels and maximum capacity: 10 x 100 mm tubes (4.5 mL) Part number: 150425 Required accessories: adapter plate (part number 2504621) Code 21 rack For 60 vessels Material: polypropylene Vessels and maximum capacity: 13 x 100 mm tubes (9 mL) Part number: 150422 Required accessories: adapter plate (part number 2504621) Code 23 rack For 44 vessels Material: polypropylene Vessels and maximum capacity: 17 x 55 mm vials (6.
Appendix B Racks Code 28 rack For 108 vessels Material: polypropylene Vessels and maximum capacity: 10 x 65 mm tubes (3 mL) 10 x 75 mm tubes (3.
B Racks Appendix Code 32 rack Thermostated rack for 60 vessels Material: aluminum Vessels and maximum capacity: 13 x 65 mm tubes (6 mL) 13 x 100 mm tubes (9 mL) Part number: 2704432 Required accessories: adapter plate (part number 2504621) and thermostating cuvette (part number 2704429) or thermostating cuvette (part number 2759550) and 832 Temperature Regulator (part number 2759502) Code 33 rack Thermostated rack for 14 scintillation vials Material: aluminum Vessels and maximum capacity: 28 x 60 mm (20
Appendix B Racks Code 201 rack For two microplates, two Eppendorf vials, and two 13 x 100 mm tubes Material: aluminum Vessels and maximum capacity: 96 well microplates Eppendorf vials (1.5 mL) 13 x 100 mm tubes (9 mL) Part number: 2504601 Code 201H rack For two microplates, two Eppendorf vials, and two 13 x 100 mm tubes; with microplate covers Material: aluminum Vessels and maximum capacity: 96 well microplates Eppendorf vials (1.
B Racks Appendix Code 204 rack For 27 scintillation tubes Material: aluminum Vessels and maximum capacity: 28 x 57 mm (20 mL) Part number: 2504604 Code 205 rack For two deep-well microplates, two Eppendorf vials, and two 13 x 100 mm tubes Material: aluminum Vessels and maximum capacity: 96 deep-well microplates Eppendorf vials (1.
Appendix B Racks Code 206 rack For 96 vessels Material: aluminum Vessels and maximum capacity: 13 x 75 mm (7 mL) Part number: 2504606 Code 207 rack For 75 vessels Material: aluminum Vessels and maximum capacity: 16 x 100 mm (12 mL) Part number: 2504607 Code 208 rack For 70 vessels Material: aluminum Vessels and maximum capacity: 18 x 150 mm (25 mL) Part number: 2504608 Code 209 rack For 96 vessels Material: aluminum Vessels and maximum capacity: 12 x 32 mm Part number: 2504609 B-7
B Racks Appendix Code 210 rack For 75 vessels Material: aluminum Vessels and maximum capacity: 16 x 75 mm (11 mL) Part number: 2504610 Code 211 rack For 9 Boston round screw-cap bottles Material: aluminum Vessels and maximum capacity: 48 x 113 mm (125 mL) Part number: 2504611 Code 212 rack For 96 vessels Material: aluminum Vessels and maximum capacity: 48 13 x 100 mm (9 mL) 48 13 x 75 mm (7 mL) Part number: 2504612 Note: Place the shorter tubes into the notched tube locations.
Appendix B Racks Code 214 rack For 96 vessels Material: aluminum Vessels and maximum capacity: 48 10.25 x 47 mm (2.5 mL) 48 10.25 x 64 mm (44 mL) Part number: 2504614 Note: Place the shorter tubes into the notched tube locations. Code 216 rack For 60 Waters WISP vials Material: aluminum Vessels and maximum capacity: 60 15 x 45 mm (4 mL) Part number: 2504616 Code 217 rack For 96 tubes Material: aluminum Vessels and maximum capacity: 96 10 x 75 mm culture tubes (3.
B Racks Appendix Code 222 rack For 27 conical bottom tubes Material: aluminum Vessels and maximum capacity: 27 (50 mL) Part number: 2504622 Code 228 rack For four reagent bottles Material: aluminum Vessels and maximum capacity: 4 (500 or 700 mL) Part number: 2504628 Code 505 rack For 10 standard or deep-well microplates Material: aluminum Part number: 2504651 Code 505H rack For 10 standard or deep-well microplates with hold-down covers Material: aluminum Part number: 2504651H B-10
Appendix B Racks Peltier Racks Code 242 Peltier rack For 2 shallow, flat-bottom, 96-well microplates Material: aluminum Vessels and maximum capacity: 2 shallow, flatbottom, 96-well microplates Part number: 25146331 Note: Order Peltier Controller (part number 2515331) and junction box (part number 2505332) separately.
B Racks Appendix Code 852 Peltier rack For two Becton Dickinson Falcon 96-well, shallow-well assay plates. U-bottom style Material: aluminum Part number: 2514852 Note: Order Programmable Peltier Controller (part number 2515850) separately. Code 853 Peltier rack For 96 12x32 mm 2 ml, flat-bottom vials Material: aluminum Part number: 2514853 Note: Order Programmable Peltier Controller (part number 2515850) separately.
GSIOC Commands C The Gilson Serial Input Output Channel (GSIOC) is an asynchronous serial communications interface that enhances the power of your Gilson equipment. The GSIOC incorporates an EIA RS-485 interface and allows up to 32 slave devices to be controlled from a single master in a multi-drop configuration. Each slave device is identified by a unique number which must be known to the device and to the computer. The default ID code of the Quad-Z 215 is 22.
C GSIOC Commands Appendix GSIOC Commands There are two kinds of commands that you can send over the GSIOC: • Buffered commands send instructions to the liquid handler. These commands are executed one at a time. • Immediate commands request status information from the Quad-Z 215. These commands are executed immediately, temporarily interrupting other commands in progress.
C Appendix I Reads non-volatile memory (NV-RAM) at current address. Returns “aaa=xxxx” where: aaa - Value between 0 and 19 for word type data or 100 to 119 for floating type data. xxxx - Data at the address. Current address is incremented. @aa[=xxxx] B Sets the value at NV-RAM address where: aa - Value between 0 and 19 for word type data or 100 to 119 for floating type data. xxxx - (Optional) Data at the address. ~n B Sets test mode depending on value assigned to n: 1 - XYZ test.
C GSIOC Commands Appendix 9 B Clears the contact event FIFO and resets the contact input event timer. A I Reads home phases for X and Y motors. Returns “xxx/yyy” where: xxx - X motor phase. yyy - Y motor phase. Bf,d B Causes 215 to beep: f - Frequency in Hz; default is 2400. d - Duration of beep in tenths of seconds; default is 1. cv=ss..ss B Sets user-definable character: v - Address (0 to 7) of user-defined character. ss..ss - Characters, in hexadecimal ASCII code, for rows 1 through 7.
C Appendix B Sets X, Y, and Z motor status: x - 0 for disable X motor. y - 0 for disable Y motor. z - 0 for disable or 1 for enable Z motor. For example, the following command disables the motors: E000. Fn B Relaxes the probes where “n” is the probe selection (a, b, c, or d). H B Moves XYZ to home position, homes all four Z-drives, and sets the X-pitch to 18 mm. I I Reads status of input contacts and front panel Start and Stop buttons.
C GSIOC Commands Appendix Ksxxx B Sets liquid level sensing sensitivity where: s - selected probe (a, b, c, or d) xxx - Value from 0 to 255; 0 is the most sensitive, 255 is the least sensitive. If s is not specified, the default is abcd. K I Reads liquid level sensing sensitivity.
C Appendix I Reads the liquid detector output. Returns “a,b,c,d” where: a - leftmost probe (probe A) b - probe B c - probe C d - probe D For each status, you’ll see A for air or L for liquid. Oabcd B Sets each probe speed value (in µm/s) where: a = speed of probe A b = speed of probe B c = speed of probe C d = speed of probe D If the value is not specified, the probe speed value will remain the same. The default speed is 125000 µm/s. O I Reads each probe speed value (in µm/s).
C GSIOC Commands Appendix Q I Reads the XYZ travel range. Returns “axis=min/max” where: axis - X, Y or Z. min - Lowest position in tenths of millimeters. max - Highest position in tenths of millimeters. The first time the command is sent, it returns the X range; the second time, it returns the Y range; the third time, it returns the Z range. R I Reads front panel LED display. Returns “cccccccc” which are the eight characters currently shown on the display.
C Appendix B Sends a GSIOC2 command directly to the Quad-Z where: c = the type of command s.ss = the command string You may send up to four GSIOC2 commands before the first command completes. The response is placed into a response buffer (one string deep) for later reading. v I Reads the response string buffer. Returns “|” if buffer is empty. Wcc..cc B Writes character string (cc..cc) to the LED display. Characters outside the printing range are shown in hexadecimal ASCII code on the display.
C GSIOC Commands Appendix Z I Reads probe Z-positions in integral units of 0.1 mm. Returns aaaa,bbbb,cccc,dddd where: aaaa is the Z-position of probe A bbbb is the Z-position of probe B cccc is the Z-position of probe C dddd is the Z-position of probe D Xax/y B Moves probe A to target x and y coordinates. Xbx/y B Moves probe B to target x and y coordinates. Xcx/y B Moves probe C to target x and y coordinates. Xdx/y B Moves probe D to target x and y coordinates.
Pipetting Techniques D By following these techniques, you’ll be able to maximize accuracy and precision, and minimize carryover, with your Quad-Z 215 Liquid Handler. Accuracy and precision of pipetting are dependent on the specifications of the pump you’re using and the geometry of the probe tip.
D Pipetting Techniques Appendix Minimizing Cross-Contamination Cross-contamination of samples is caused by sample carryover on the probe tip or on its inner walls. Several methods are available to minimize cross-contamination. • Use of the liquid level sensing capability will reduce carryover by limiting immersion below the liquid surface. • Cleansing the probe tip with a flowing rinse removes contaminants from the external surfaces of the probe.
Appendix D Pipetting Techniques Minimizing Dilution Effect An undesirable dilution sometimes occurs when an aspirated sample replaces rinse or diluent material. Some of the diluent fluid invariably clings to the tubing walls, causing the unwanted dilution of the sample. The part of the sample that enters the tubing first is affected most because it is the closest to the diluting liquid. The corrective action you take depends on the stringency of your requirements for sample purity and precision.
Appendix D Pipetting Techniques The following example describes an instance when the above 8-step procedure is required. An operator needs to dispense 50 µL of reagent into each of 20 test tubes. The tube-to-tube consistency of reagent volume is critical. However, in order to save time, the operator wishes to make a single 1 mL aspiration from the reagent reservoir and then make multiple dispenses.
