User guide

M
Millenium™ RO System by GE Osmonics
14693e - 31 Mar 03 4- 27 Routine Maintenance
4.5.1 Product Water Culture Procedure
This procedure assumes the RO is connected to power and water, and is in operating
condition.
1. Connect the product rinse line to the product port. Place the other end in a drain.
2. Connect the waste line to the waste port. Place the other end in a drain.
3. Turn on the RO unit. Allow the RO unit to run for 10-15 minutes.
4. Remove the product rinse line from the drain.
5. Using aseptic technique, hold the product line over a container, and allow
approximately 100 cc of product (RO) water to flow to the drain (or allow product
water to flow for 30 seconds).
6. Maintaining aseptic technique, hold the product line over the sterile container
with the product water flowing.
7. Fill the container with RO product water. (If using commercial samplers, follow
the manufacturer's instructions.)
8. Aseptically cap the specimen container immediately.
9. Label the specimen appropriately with:
• Test to be performed - "culture / colony count"
• Sample source - product water/RO, machine serial number and room location
• Time and date sample obtained
• Person who obtained specimen
• Any other pertinent information or procedures your facility or lab requires
10. The samples should be assayed within 30 minutes of obtaining or refrigerated at
5° C immediately and assayed within a 24-hour period.