Part No.: H-020-000137-00 DNB Loader MGIDL-T7RS Version: 3.0 Address: Building 24, Stage 3.1, BioLake Accelerator, No.388, 2nd Gaoxin Road, East Lake High-Tech Development Zone, 430075, Wuhan, P.R.China E-mail: MGI-service@mgi-tech.com Website: www.mgi-tech.com Research Use Only Wuhan MGI Tech Co., Ltd.
About the user manual This user manual is applicable to MGIDL-T7RS DNB Loader. The manual version is 3.0 and the software version is V1. This manual and the information contained within are proprietary to Wuhan MGI Tech Co., Ltd. (hereinafter called MGI), and are intended solely for the contractual use of its customer in connection with the use of the product described herein and for no other purpose.
Revision history Date Version May 24, 2022 3.0 February 22, 2021 2.0 December 30, 2020 1.
Contents Safety 1 Conventions 2 General safety 2 Electrical safety 3 FCC statement 4 IC statement 5 Mechanical safety 5 Components safety 5 Biological safety 6 Symbols 6 Device 6 Label 7 Packaging 9 Device overview 11 Intended use 12 Working principle 12 Structural composition 12 Front view 13 Back view 15 Flow cell stage A 16 Flow cell 17 Plate tray unit 17 Control software 18 Self-test interface 18 Main interface 19 Log interface 21 Setting
Contents Shutdown or restart interface 22 About interface 22 Getting started 23 Site requirements 24 Network requirements 24 Operating environments of the computer 24 Software security 25 Data and device ports 25 Inspecting the device 25 Preparing the power supply 26 Installing the device 26 Powering on or off the device 27 Powering on the device 27 Powering off the device 27 Preparing materials for post wash 27 Material list 27 Preparing the post-loading pla
38 Monthly cleaning 38 Service plan 39 Maintaining the power supply 39 Maintaining the flow cell stage 39 Tools and solutions 39 Cleaning the flow cell stage 39 Replacing the sealing ring 40 Maintaining the software 41 Troubleshooting 41 Storage and transportation 43 Disposal of the device 44 Specifications 45 Compliance information 47 Manufacturer information 49 European representative information 51 Index 53 III Contents Weekly cleaning
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Safety This chapter describes basic safety information about the device. Carefully read and understand the information before use to ensure correct operations, best performance, and personnel safety. Keep this manual at hand for reference at any time.
Safety Conventions The following table describes conventions that are used in the manual: Item Description Indicates that the operator should operate the device by following the instructions. Otherwise, it might result in personal injury. Indicates that the operator should operate the device by following the instructions. Otherwise, it might result in device malfunction, damage or inaccurate experiment result.
and debugged the device. Incorrect operation will result in inaccurate experiment result. If you require to re-position the device, contact the technical support. z Only trained professionals such as doctors, technicians, or laboratory assistants can operate the device. z Do not operate the device in the presence of flammable or explosive liquids, vapors or gases. Otherwise, it might result in device malfunction. z Do not disconnect the power cord under the power-on status.
Safety z Only the power cord supplied by the manufacturer can be used. z Prepare the laboratory and power supply according to Getting started on Page 23 . z Ensure that the input voltage meets the device requirement. FCC statement This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: 1. This device may not cause harmful interference, and 2. This device must accept any interference received, including interference that may cause undesired operation.
This device complies with Industry Canada’s licence-exempt RSSs. Operation is subject to the following two conditions: 1. This device may not cause interference; and 2. This device must accept any interference, including interference that may cause undesired operation of the device. The distance between user and products should be no less than 20 cm. Le présent appareil est conforme aux CNR d’Industrie Canada applicables aux appareils radio exempts de licence.
Safety z Do not uninstall the control software by yourself. If any problem occurs during software operation, contact the technical support. z Ensure that the peripheral devices meet the IEC/EN 60950-1 standards. Biological safety z Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa.
Name Warning; dangerous voltage Description Indicates hazards arising from dangerous voltages. Indicates the terminal of a Protective earth protective earth (ground) electrode. F10AL250V Fuse specification Indicates the fuse specification. RFID (Radio frequency RFID identification) reader Identifies the ID of the item placed near the area. indication SWITCH Power switch Powers on or off the device. SOCKET Power port Connects to the mains supply.
Safety Symbol Name Date of manufacture Description Indicates the date when the medical device was manufactured. Indicates the manufacturer’s SN Serial number serial number so that a specific medical device can be identified. CE Mark of Conformity Indicates that this device conforms with the specified Council Directive.
The following table describes symbols on the packaging or on the label of the packaging: Symbol Name Description Indicates the correct upright This way up position of the transport package for transport and/ or storage. Fragile, handle with care Indicates a medical device that can be broken or damaged if not handled carefully. Indicates a medical device that Keep dry needs to be protected from moisture.
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Device overview This chapter describes the intended use, working principle, and structural composition of the device.
Intended use Device overview The device is used to prepare sequencing flow cells by loading the pre-prepared DNBs (DNA Nanoball) and/or reagent to a flow cell. This device is intended only for scientific research and should not be used for clinical diagnosis. Working principle The device loads the sample libraries and/or reagent to a sequencing flow cell through defined and optimized vacuum procedures.
Front view Structural composition 1 13 2 3 5 4 6 7 R FI D 8 9 10 11 12 Figure 1 Front view No. Name 1 Touch screen monitor 2 Flow cell compartment Holds the flow cell. Flow cell compartment Pushes it right or left to open or close door the flow cell compartment door. 3 4 Loading compartment Description Facilitates the on-screen operation and displays information. Places the post-loading plate and load the liquid.
No. Name Description Pushes it right or left to open or close Device overview the loading compartment door. 5 Loading compartment door When opening or closing the left compartment door, handle with care in case of injury to hands. Displays the current status of the device. z Blue: the device is in standby status. 6 LED status bar z Green: the device is running. z Yellow: a warning appears. z Red: an error occurs.
Back view Structural composition 1 2 Figure 2 Back view No. Name Description 1 Ventilation outlet Ventilates the device. 2 Supporting feet Supports the main unit to ensure stability.
Flow cell stage A Device overview 4 3 1 2 Figure 3 Flow cell stage A The following description uses flow cell stage A as an example. No. Name Description Flow cell attachment Press to activate the vacuum for button A attachment or release of the flow cell. 2 Aluminum chuck Loads and attaches the flow cell. 3 Sealing ring / 4 Alignment groove Used for aligning with the flow cell.
Flow cell 1 Structural composition Figure 4 Flow cell No. Name Description 1 Label location Posts the label of the flow cell. Plate tray unit 2 1 Figure 5 Plate tray unit No. Name Description 1 DNB tube hole Holds the DNB tube. Holds the post-loading plate and brings 2 Plate tray it to the specified position while Y-Z motion stage moves.
Control software Device overview The software of the device can guide the user to load different sample libraries and/or reagents to a sequencing flow cell according to experimental requirements. The following table describes the function of each functional module: Item Self-test Loading Wash Description Checks whether the components of the system are functional. Loads the required sample library and/or reagent to the flow cell. Performs wash and maintenance for the fluidics lines of the system.
Main interface 1 Zlims Control software B A 2 Figure 6 Main interface The main interface includes the following areas. No. Name Icon and button 1 area 2 Operation area Description Displays the icon and buttons. Select a flow cell stage and perform the relevant operations. If you select flow cell stage A, the main interface A appears. A B x.xx kPa x.
The following table describes the function of controls in the interface: Device overview Item Description Displays the temperature of the flow cell stage. Displays the negative pressure. Icon and button area The following table describes the function of controls in the interface: Item Description Displays the local date and time. Tap to return to the main interface. Zlims Displays the connection status of the device and the server that ZLIMS is installed on. Tap to view prompt details.
Item Description The flow cell stage is under loading. Control software The fluidics lines of the flow cell stage are under washing. The flow cell stage is in idle status. Loading or washing is being paused. Log interface You can view the log in this interface. To open the log interface, tap in the main interface, and select Log. The following table describes the function of controls in the interface: Item Description Tap to exit the log interface and return to the previous interface.
To open the settings interface, tap , and select Settings. Device overview The following table describes the function of controls in the interface: Item Language Network Customize Description Tap to change the language of the software. Restart the device to apply the changes. Input the IP address and port number of the ZLIMS server. Modification takes effect after you restart the software. Move the slider to change the volume of the speaker.
Getting started This chapter describes laboratory preparations and loading preparations.
Site requirements z Ensure that the laboratory floor is level and the laboratory table has a weight capacity of over 200 kg (441 lb). z Ensure that the laboratory is free of dust, corrosive and flammable gas, and Getting started heat and wind sources. z Ensure that the laboratory is away from direct sunlight and well ventilated. We recommend that you refer to the standard of a biosafety level (BSL) 2 laboratory.
Software environments Only the software that has been provided by the manufacturer can be installed and used on the computer. Because unknown software might interfere with normal device functions, or even cause data loss. If you need to install antivirus software, contact the technical support in advance. Software architecture: C/S Inspecting the device Pre-installed software on the computer includes: z Control software z Microsoft Windows 10 64 bits operating system z Microsoft .Net Framework 4.6.
The following table describes indicator statuses on the label: Table 1 Indicator status Label Indicator status Description Getting started Indicates that the device is intact Remains and no strong collision occurs during unaffected transportation, or the intensity does Anti-shock not exceed the limit. label Indicates that the device might not be intact and a strong collision occurs Red during transportation and the intensity exceeds the limit.
Powering on or off the device z Ensure that the power switch is in the position before connecting to the power supply. z The mains supply socket should be a standard three-prong socket and grounding cable of the power supply system. If the requirements above are not met, the device must be protectively grounded as described in Preparing the power supply on Page 26 .
z Washing flow cell (delivered with the device) Preparing the post-loading plate Perform the following steps: Getting started 1. Remove the sealing film of the post-loading plate. 2. Add 4 mL of 0.1 M NaOH that is used for washing to well 11 by using the pipette. Figure 9 Adding 0.1 M NaOH 3. Place the prepared post-loading plate close to the device.
Loading This chapter describes the loading workflow and wash procedures. The following description uses flow cell stage A as an example. Read and follow the instructions to ensure correct operations.
Workflow Preparing sample libraries and materials Loading consumables Loading Performing a post wash Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing an experiment.
¾ Tap , and a confirmation message appears. Select Yes to pause the wash. Tap again to resume the wash. ¾ Tap , and a confirmation message appears. Select Yes to stop the wash. 5. When the wash is complete, take out all the consumables by following the on-screen instructions. 6. Tap Back to return to the main interface A. 7. Store the washing flow cell at the room temperature. 8. Empty any remaining washing solution in the washing plate into an appropriate waste container.
If scanning fails, input the plate ID with the on-screen keyboard. z Ensure that the ID format is correct when you input ID manually. Otherwise, you will be prompted that the ID is incorrect and the procedure cannot continue. z The plate ID consists of 10-digit catalogue numbers and 11-character serial numbers. Loading 3. Load the post-loading plate onto the plate tray in the direction of the arrow shown in the following figure. After that, you will be prompted that the post-loading plate is loaded.
2) Press the left and right edges and ensure that the flow cell is completely aligned with the flow cell stage. 2 1 Figure 12 Loading the flow cell 9. Press the flow cell attachment button A. If the indicator in the flow cell attachment button illuminates in green, the vacuum pump works normally. And you will be prompted that the flow cell is loaded. After the flow cell is securely seated, do not move it again.
Performing a load z Ensure that the loading compartment door is closed before loading. Loading cannot be started when the loading compartment door is open. z Do not open the loading compartment door during a loading so as to prevent adverse effects on loading results and damage to the device. z Do not bump, move, vibrate or impact the device during a loading because it might affect the results.
After the loading is complete, perform the following steps: 1. Select Yes, the loading completed interface is displayed. A B Loading complete. x.xx kPa x.xx °C Please replace the flow cell with a washing flow cell. A: Idle Post-wash Figure 13 Loading completed interface 2. Remove the sequencing flow cell by following the on-screen prompt. 1) Open the flow cell compartment door. 2) Press the flow cell attachment button A. 3) Press the lower edge of the flow cell to raise the flow cell.
2. Close the flow cell compartment door. 3. Tap Post-wash and a confirmation message appears. Select Yes to start post-washing. ¾ Tap , and a confirmation message appears. Select Yes to pause the wash. Tap again to resume the wash. ¾ Tap , and a confirmation message appears. Select Yes to stop the wash. Loading 4. When the wash is complete, take out all the consumables by following the on-screen instructions. 5. Tap Finish to return to the main interface A. 6.
Maintaining the device This chapter describes maintenance procedures of the device and its parts. Perform maintenance regularly to ensure that the device runs smoothly.
Maintaining the main unit Daily maintenance Perform the following maintenance when the device is powered on: z Check whether the fan of the device is operational. If not, contact the technical support. Maintaining the device z During loading, pay attention to error messages and check whether the relevant parts can work properly. Contact the technical support if needed. For information about how to find error messages, refer to Log interface on Page 21 .
Service plan Contact the technical support for paid maintenance services if needed. We offer free first-year warranty and multi-price extended service plans. For details, contact the technical support. Maintaining the power supply z When the device is not in use for seven days or longer, perform a maintenance wash, power off the device, and disconnect the power cord. Maintaining the flow cell stage Perform cleaning and maintenance for the flow cell stage before use.
3. If necessary, wipe the back of the flow cell or the surface of the aluminum chuck with a dust-free cloth moistened with absolute alcohol, and then let it air-dry. Do not wipe the inlet holes and vacuum attachment slot, to prevent the absolute alcohol from entering the holes and damaging the device. 4. Use a dust remover to carefully blow particulate matter and dust from the surface of the silicon chip and aluminum chuck until they are clean. 5. Place the flow cell on the flow cell stage.
3. Install the new sealing rings to the flow cell stage in the direction of the arrow shown in the following figure. 4. Press the sealing ring with the finger point. Ensure that the sealing rings are securely and evenly installed. Maintaining the software After the receival of Release Notification , contact the technical support to update the software. Troubleshooting If malfunction occurs during operation, the device beeps or a message appears on the screen.
Table 2 Troubleshooting Problem Possible cause Recommended action 1. Close the compartment Compartment door opened is displayed in the interface. The compartment door is opened. door again. 2. If the problem persists, contac t the technical support. The sampling needle bumps into the post-loading plate and bends Position settings are Contact the technical improper. support to set the position. Maintaining the device during operation. 1.
Problem Possible cause Recommended action 1. Check whether the sealing ring on the flow cell stage z There are foreign is intact or any foreign particles might particles block the holes b e p re s e n t o n of the sealing ring. the sealing ring 2. C h e c k w h e t h e r t h e re The liquid does not or the sealing are any foreign particles p a s s t h ro u g h t h e ring is damaged. on the rear of the flow fluidics lines of the z There are foreign flow cell.
z If you want to move or transport the device, contact the technical support. Disposal of the device Maintaining the device The service life of this device is seven years, which is determined by the simulated service life evaluation method. For the date of manufacture, refer to the label on the device. Perform the maintenance according to the requirements mentioned in this manual. Dispose of the end of life device according to local regulations.
Specifications Item Dimension Net weight Description 430 mm (W) × 750 mm (H) × 780 mm (D) (17 inches × 30 inches × 31 inches) Approximately 81 kg (179 lb) z Type: LCD touch screen Touch screen monitor z Size: approximately 13.3 inches z Resolution: 1280 × 600 pixels Specifications z Voltage: 100 V - 240 V~ z Frequency: 50/60 Hz Power z Rated power: 600 VA z Overvoltage category: II z Cable: min.
Item Description z Temperature: 19 °C to 25 °C (66 °F to 77 °F) z Relative humidity: 30% RH to 80% RH, non-condensing z Atmospheric pressure: 80 kPa to 106 kPa z Altitude: ≤2000 m z Pollution degree: 2 O p e r a t i n g e n v i ro n m e n t z Indoor use requirements Because the temperature and humidity fluctuations influence the accuracy of the loading results, we recommend that you install an air conditioning system and a Specifications dehumidifier in the laboratory to maintain appropriate temperatu
Compliance information The device complies with following standards: Item Electromagnetic Compatibility (EMC) Standard IEC 61326-1:2020 Electrical equipment for measurement, control and laboratory use – EMC requirements – Part 1: General requirements z IEC 61010-1:2010+AMD1:2016 z IEC 61010-2-081:2019 Safety requirements Safety requirements for electrical equipment for measurement, control and laboratory use - Part 2-081: Particular requirements for automatic and semi-automatic laboratory equipment for a
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Manufacturer information Manufacturer Wuhan MGI Tech Co., Ltd. Building 24, Stage 3.1, BioLake Accelerator, Address No.388, 2nd Gaoxin Road, East Lake High-Tech Development Zone, 430075, Wuhan, P.R.China Technical support Technical support E-mail Wuhan MGI Tech Co., Ltd. MGI-service@mgi-tech.
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European representative information Name Latvia MGI Tech, SIA Address Dzirnavu street 57a-4, Rīga, Latvia, LV-1010 European representative information 51
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Index A anti-shock and anti-tilt labels 23 Anti-shock label 24 Anti-tilt label 24 B Index Biological safety 4 C computer 4 control software 4 D device damage 2 E electromagnetic environment 3 empty the fluidics line 20 F fluidics lines 3 fuse 4 53
L Loading compartment 11 M Main interface 17 N Network requirements 22 Index P Plate tray unit 15 Power switch 12 pre-run 28 R re-position 3 RFID 6 RFID scanning area 12 54
