Users Manual Part 2

Maintaining the device
50
Performing a wash
Chemicals in reagents and waste might cause personal injury through
contact with the skin, eyes, and mucosa. Follow the safety standards of your
laboratory and wear protective equipment (such as laboratory coat, and
disposable bouffant cap, protective glasses, mask, gloves, and shoe covers)
when performing a sequencing run.
Warning
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We do not recommend that you use other disinfectants or wash solutions
except for those that are mentioned in this manual. Other solutions are not
verified for use and their effects to the device are unknown.
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If you have questions about the compatibility of wash solutions, contact the
technical support.
You can perform a wash to remove the remaining reagents from the
fluidics lines and flow cell stages, and avoid cross-contamination.
When Auto wash is enabled, the system automatically performs a
wash after each sequencing run. If Auto wash is set to No or the
device is not used for 7 days or longer, perform a wash manually.
Wash solutions that can be used include:
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1 M NaCl and 0.05% Tween-20
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0.1 M NaOH
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Laboratory-grade water
You can use laboratory-grade water such as 18 Megohm (MΩ) water, Milli-Q
water, Super-Q water, or similar molecular biology-grade water.
Perform the following steps:
1. Before sequencing, log in to your account. Tap Wash in the main
interface of the flow cell stage.
2. Prepare the washing cartridge.
For details about preparing the reagent cartridge, refer to the
relevant reagent kit user manual.
3. Follow the on-screen steps to load the washing flow cell, washing
cartridge, and an empty sequencing reagent cartridge. Close all
compartment doors.
For details, refer to Loading the flow cell on Page 41 and
Loading the reagent cartridge on Page 41 .
4. In the wash interface, tap Wash and a confirmation dialog box
appears. Select Yes to start wash.
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Tap to pause, and tap it again to resume wash.