Preparing the pure water container 1. Ensure that the pure water contained is sufficient. Warning Insufficient pure water will result in sequencing failure. Getting started For details about pure water consumption, refer to the relevant reagent kit user manual. For details about maintaining the pure water container, refer to Maintaining the pure water container on Page 51. 2. Connect the pure water container to the device. 1) Place the fixing plate on the lid and align the holes.
3) Open the airway. 3. If you need to add pure water into the pure water container during sequencing, perform the following steps: 1) Open the lid in position 1 according to the direction indication on the lid. 2) Insert the water output tube of the pure water machine into the pure water container through the water inlet in position 2. 1 2 Figure 17 Adding pure water during sequencing 3) Fill the pure water container with fresh pure water.
Getting started For details about estimating the space, refer to the relevant reagent kit user manual. For details about replacing the waste container, refer to Replacing the waste container on Page 51.
Sequencing This chapter describes the sequencing workflow and washing procedures. Read and follow the instructions to ensure correct operations.
Workflow Log in ABCD Select a flow cell stage Sequencing Load the reagent cartridges and flow cell Review parameters Perform a sequencing run Automatic post-wash z Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing a sequencing run.
4. Log in to the control software with the user name and password that are provided by the manufacturer. Selecting a flow cell stage In the operation area of the main interface, select a flow cell stage. Loading the reagent cartridge 1. Prepare the reagent cartridges, including the sequencing cartridge and washing cartridge. For details about preparing the reagent cartridge, refer to the relevant reagent kit user manual. 2. Open the reagent compartment door. 3.
A B C D Sequencing cartridge ID REF+SN, e.g. 1000000000S0000000000 Washing cartridge ID REF+SN, e.g. 1000000000S0000000000 Flow cell ID xxxxxxxx Recipe SE100+10 1-128 Split barcode Sequencing Advanced settings Previous Next A: Preparing Figure 18 Sequencing information input interface z Ensure that the ID format is correct when you input ID manually. Otherwise, you will be prompted that the ID is incorrect and the procedure cannot continue.
6. Tap Advanced settings and you can enable Custom primers and Auto wash if needed. In the sequencing process, you can tap to enable or disable Auto wash if needed. 7. Tap Next. If any error prompts occur, follow the on-screen prompt to solve the problem and tap Next again. Carefully check each item in the review interface, and do one of the following: z If you find any error, tap Previous to return to the previous interface and reset parameters. z If all parameters are correct, tap Start.
The following table describes the function of each item in the interface: Item Description Estimated completion time Shows the estimated time when sequencing completes. QC type You can select a QC value graph from the QC type list to assess the sequencing quality. Sequencing Tap it and a confirmation dialog box appears. Select Yes to pause sequencing. Tap it again and a confirmation dialog box appears. Select Yes to resume sequencing. Tap it and a confirmation dialog box appears.
2. Open the flow cell retrieval compartment and remove the flow cell. Used sequencing flow cells function as washing flow cells. The washing flow cell can be reused for three times. Dispose of the flow cell according to local regulations and safety standards of your laboratory. 3. Open the reagent compartment door and remove the reagent cartridges. 5. Dispose of the flow cell and reagent cartridges in accordance with local regulations and safety standards of your laboratory.
---This page is intentionally left blank.
Maintaining the device This chapter describes maintenance procedures of the device and its parts. Perform maintenance regularly to ensure that the device runs smoothly.
Maintaining the main unit Daily maintenance z Perform the following maintenance when the device is powered on: ¾ Check whether the fan at the rear of the device is operational. If not, contact the technical support. Maintaining the device ¾ During sequencing, pay attention to error messages and check whether the relevant parts can work properly. Contact the technical support if needed. For information about how to find error messages, refer to Menu area on Page 23 , and Log interface on Page 25 .
Monthly cleaning Warning z We do not recommend that you use other disinfectants or wash solutions except for those that are mentioned in this manual. Because other solutions are not verified for use and their effects to the device are unknown. z If you have questions about the compatibility of wash solutions, contact the technical support. Perform the following steps: 1. Power off the device and open the reagent compartment.
Performing a wash Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing a sequencing run. Warning z We do not recommend that you use other disinfectants or wash solutions except for those that are mentioned in this manual.
¾ Tap and a confirmation dialog box appears. Select Yes to stop wash. 5. After wash, tap Finish to return to the main interface. 6. Remove the washing flow cell, sequencing cartridge, and washing cartridge. 7. Store the washing flow cell at room temperature. The washing flow cell can be reused for only one month. Dispose of the flow cell according to local regulations and safety standards of your laboratory. 8. Empty the remaining solution in the washing cartridge into an appropriate waste container.
The waste container is connected to the device through tubes. To avoid liquid leakage and biological hazard, monitor the waste container status frequently and dispose of the waste and waste container in time when the waste reaches the maximum volume. Maximum volume Maintaining the device Figure 20 Maximum waste volume Perform the following steps: 1. Wear protective equipment. 2.
Problem Possible cause Recommended action 1. Perform a self-test in the maint enance int er f ace. z The parameters are not set Error messages properly. Check the record of the hardware that fails the selftest. 2. C h e c k e r ro r m e s s a g e s appear when the z Errors occur in the log, and solve the control software when the problem according to on- runs. software screen instructions. communicates with hardware. 3. Restart the device. 4. If the problem persists, support.
Disposal of the device Maintaining the device The service life of this device is seven years, which is determined by the simulated service life evaluation method. For the date of manufacture, refer to the label on the device. Perform the maintenance according to the requirements mentioned in this manual. Dispose of the end of life device according to local regulations. Or, if the device is confirmed that it still can work safely and effectively after maintenance, keep the device.
Appendixes This chapter provides additional important information about the device.
---This page is intentionally left blank.
Specifications Item Laser classification of the device Dimensions Net weight Power Class 1 laser product 1656 mm (W) × 1815 mm (H) × 903 mm (D) (65.2 inches × 71.5 inches × 35.6 inches ) Approximately 765 kg (1687 lb) Type LCD Size 20 inches Resolution 1920 × 1080 pixels Voltage 200 V to 240 V~ Frequency 50/60 Hz Rated power 3000 VA Overvoltage category Cable Maximum sound pressure level Specifications Touch screen Description II min.
Item Description Temperature Relative humidity Atmospheric pressure Operating environment requirements 19 ℃ to 25 ℃ (66 ℉ to 77 ℉ ) 30% RH to 80% RH, noncondensing 80 kPa to 106 kPa Altitude ≤2000 m Pollution degree 2 Indoor use Because the temperature and humidity fluctuations influence the accuracy of the experiment results, we recommend that Specifications you install an air conditioning system and a humidifier or dehumidifier in the laboratory to maintain the temperature and humidity.
Compliance information The device complies with the following standards: Item Electromagnetic Compatibility (EMC) Standard IEC 61326-1:2020 Electrical equipment for measurement, control and laboratory use – EMC requirements – Part 1: General requirements z IEC 61010-1:2010+AMD1:2016 z IEC 61010-2-081:2019 Safety requirements Safety requirements for electrical equipment for measurement, control and laboratory use - Part 2-081: Particular requirements for automatic and semi-automatic laboratory equipment
---This page is intentionally left blank.
Manufacturer information Manufacturer Wuhan MGI Tech Co., Ltd. Building 24, Stage 3.1, BioLake Accelerator, Address No.388, 2nd Gaoxin Road, East Lake HighTech Development Zone, 430075, Wuhan, P.R.China Technical support Wuhan MGI Tech Co., Ltd. Technical support E-mail MGI-service@mgi-tech.
---This page is intentionally left blank.
European representative information Name Latvia MGI Tech, SIA Address Dzirnavu street 57a-4, Rīga, Latvia, LV-1010 European representative information 63
---This page is intentionally left blank.
Index A anti-shock and anti-tilt labels 33 antivirus software 31 B Biological safety 6 Index C combinatorial probe-anchor synthesis (cPAS) 12 E empty the fluidics line 27 F Flow cell 18 Flow cell drive 16 Flow cell drive button 15 Flow cell retrieval compartment 16 H Host power button 17 L LED status bar 16 65
M Main interface 22 Maintenance 27 N Network requirements 30 P Performing a wash 50 Washing cartridges 35 Index peripheral devices 34 Pollution degree 58 Power switch 21 R Reagent cartridge and sequencing flow cell 35 Reagent compartment 18 S safety information 1 Self-test 21 sensor status indicators 24 Settings interface 26 T Troubleshooting 52 W Workflow 40 66