Instruction Manual DO 300 Waterproof Hand-held Dissolved Oxygen / Temperature Meter 68X248909 Technology Made Easy ...
Preface This manual serves to explain the use of the Waterproof DO 300 hand-held meter. It functions in two ways, firstly as a step by step guide to help you to operate the meter. Secondly, it serves as a handy reference guide. It is written to cover as many anticipated applications of the Waterproof DO 300 meter as possible. If there are doubts in the use of the meter, please do not hesitate to contact the nearest Authorised Distributor.
TABLE OF CONTENTS 1 INTRODUCTION 2 DISPLAY AND KEYPAD FUNCTIONS 2.1 2.2 3 3.1 3.2 4 4.1 4.2 4.3 4.4 5 5.1 5.2 Display Keypad Inserting the Batteries Connecting the Probe CALIBRATION Preparing the meter for calibration Temperature Calibration Dissolved Oxygen Calibration in % Saturation Dissolved Oxygen Calibration in mg/L (ppm) mode MEASUREMENT Taking DO readings Taking Pressure/Salinity Compensated DO measurements HOLD FUNCTION 7 MEMORY FUNCTIONS 8 8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8 8.9 8.
Instruction Manual 1 DO 300 INTRODUCTION Thank you for selecting the DO 300 waterproof portable meter. This meter is a microprocessor-based instrument that is designed to be handy and capable of allowing onehand operation. It is capable of measuring Dissolved Oxygen in mg/L or ppm, % Saturation of Oxygen and Temperature. It is completely WATERPROOF --- and it FLOATS! This meter has many user-friendly features ---- all of which are completely accessible through the water-resistant membrane keypad.
Instruction Manual 2 DO 300 DISPLAY AND KEYPAD FUNCTIONS 2.1 Display The LCD has a primary and secondary display. o The primary display shows the measured DO value either in mg/L, ppm or %, depending on units of measurement selected. o The secondary display shows the measured temperature in °C. The display also shows error messages, keypad functions and program functions. See Figure 2. Figure 2: Full LCD Screen 1. SETUP mode indicator 8. Temperature indicator 14. Probe indicator 2.
Instruction Manual 2.2 DO 300 Keypad The large membrane keypad makes the instrument easy to use. Each button, when pressed, has a corresponding graphic indicator on the LCD. See Figure 1. Some buttons have several functions depending on its mode of operation. Key Function ON/OFF Powers meter on and off. The meter powers on in the mode that you were last using. For example, if you shut the meter off in mg/L measurement mode, the meter will be in mg/L measurement mode when switched on.
Instruction Manual 3 3.1 DO 300 PREPARATION Inserting the Batteries Four AAA batteries are included with your meter. 1. Use a Philips screwdriver to remove the two screws holding the battery cover. See Figure 3 below. 2. Remove battery cover to expose batteries. 3. Insert batteries. Follow the diagram inside the cover for correct polarity. 4. Replace the battery cover into its original position and secure into place using the two screws removed earlier.
Instruction Manual 3.2 DO 300 Connecting the Probe Your meter uses a dissolved oxygen/temperature probe. The probe cable has a notched 6-pin connector to attach the probe to the meter. NOTE: Do not substitute other probes. For a replacement probe, see the “Accessories” section, page 47- 48. NOTE: Keep connector dry and clean. Do not touch connector with soiled hands. To connect the probe: 1. Line up the notch and 6 pins on the meter’s connector with the holes in the probe’s connector.
Instruction Manual 4 4.1 DO 300 CALIBRATION Preparing the meter for calibration The amount of oxygen dissolved in water will depend on its temperature, atmospheric pressure and its salinity. While the pressure and salinity values are manually entered into the instrument, the temperature is being measured by the probe. It is therefore very important that the temperature is calibrated if necessary prior to the DO calibration.
Instruction Manual 4.2 DO 300 Temperature Calibration DO in mg/L is dependent on temperature, so it is first necessary to verify the temperature reading. Temperature calibration is accessible from the mg/L or ppm measurement mode. The built-in temperature sensor in the probe is factory calibrated. Calibrate your sensor only if you suspect temperature errors may have occurred over a long period of time or if you have a replacement probe. 1. Dip the DO probe into a solution with known temperature.
Instruction Manual 4.3 DO 300 Dissolved Oxygen Calibration in % Saturation You can calibrate this meter quickly and easily in air. The exact calibration value depends on barometric pressure. The meter is set to a factory default of 760 mm Hg, which results in a calibration value of 100% saturation in air. NOTE: If the barometric pressure setting has been changed from 760 mm Hg, the calibration value in air will automatically adjust to a value other than 100%.
Instruction Manual DO 300 To calibrate 0% Saturation: 1. Rinse the probe well with DI rinse water. For best MEAS accuracy, wipe the end of the probe dry. Do not touch the membrane. 2. 3. % 8.5 READY 23.4 Press the MODE key to select the % saturation mode. °C ATC Place the probe in zero oxygen solution. Stir the solution L CA AS ME gently using the probe to homogenize the sample. Make sure that the sample is continuously flowing past the membrane sensor. CAL % 4. Press the CAL key.
Instruction Manual 4.4 DO 300 Dissolved Oxygen Calibration in mg/L (ppm) mode Calibrating the meter to 100% in saturation mode will also calibrate the mg/L mode at the value in mg/L corresponding to 100% saturation. This should produce acceptable results in most applications. This meter also lets you make a calibration adjustment in mg/L (ppm) mode without affecting your % saturation calibration. To select between mg/L and ppm units, see page 30. To calibrate in mg/L (ppm) mode: 1.
Instruction Manual 5 DO 300 MEASUREMENT 5.1 Taking DO readings During measurement, the probe can be: z Fully immersed in the solution z Partially immersed in the solution Do not allow the probe’s membrane surface to touch anything! It is always advisable to insert the probe guard (refer to Figure 34 and 35 on pages 38 and 39) IMPORTANT: Since the DO probe consumes oxygen from the sample, the sample must constantly flow past the membrane to achieve more accurate readings.
Instruction Manual DO 300 NOTE: To select between mg/L and ppm units, see Section 8.7.2 on page 30. The ATC indicator should appear in the lower right hand corner of the display. If it does not, this indicates an error. Taking measurements with READY ON indicator selected on If the READY indicator has been activated, the READY annunciator lights when the reading is stable. To enable or disable the READY indicator – see Section 8.7.1 on page 29 for directions.
Instruction Manual 5.2 DO 300 Taking Pressure/Salinity Compensated DO measurements If necessary, you can adjust the pressure and salinity values of your measurements in the SETUP mode. The DO meter will automatically compensate for salinity and pressure based on the values entered in the SETUP functions. The meter is factory set at 760 mm Hg (101.3 kilo Pascals) pressure adjustment and a factor of 0.0 ppt salinity adjustment. See Section 15 on page 50 for a “Pressure vs Altitude” table.
Instruction Manual 6 DO 300 HOLD FUNCTION This feature lets you freeze the dissolved oxygen and temperature readings for a delayed observation. HOLD can be used any time when in MEAS mode. To hold a measurement, press the HOLD key while in measurement mode. “HOLD” will appear on the display. To release the held value, press HOLD again. Continue to take measurements. NOTE: This meter shuts off automatically 20 minutes after the last key pressed.
Instruction Manual 7 7.1 DO 300 MEMORY FUNCTIONS Memory Input Your meter stores data in sets that include: z Dissolved oxygen with corresponding temperature reading z Time and date reading was taken You can store up to 50 sets of data in any combination of %, mg/L or ppm readings. To store a reading: 1. During any measurement function (MEAS), press the MI key to input any data into the memory. 2. MEM, “StO” and memory number will flash.
Instruction Manual 7.2 DO 300 Memory Recall This function recalls the previous readings stored in the memory. You can only access MR from the MEASurement mode. Memory recall is in “Last In First Out” order. To recall readings: 1. Press the MR/ key once to retrieve the last reading stored. The memory location screen “MEM”, “LOC” and the memory location number will be displayed. 2. Press the ENTER key to recall the reading stored under that memory location number. 3.
Instruction Manual 8 DO 300 ADVANCED SETUP FUNCTIONS Your waterproof meter advanced SETUP mode lets you customize your meter parameters. It’s menu features different main groups for various parameter preferences. This meter will have two sets of SETUP mode; one set is when SETUP mode is entered from % saturation or Clock mode (% saturation SETUP mode) and the other set is when SETUP is entered from the mg/L (ppm) measurement mode (mg/L (ppm) SETUP mode).
Instruction Manual 8.1 DO 300 Advanced SETUP Mode Detailed Overview Press the SETUP key to enter SETUP mode. Press MI/ or MR/ keys to scroll through main groups. mg/L (ppm) Measurement Mode CLr: Memory Clear • Clear all stored readings Instructions on page 20. dPr: Dissolved Oxygen parameters • Select Hg or Pa barometric pressure units • Select barometric pressure • Select salinity adjustment factor Instructions on page 21.
Instruction Manual DO 300 % saturation Measurement Mode CLr: Memory Clear • Clear all stored readings Instructions on page 20. dPr: Dissolved Oxygen parameters • Select Hg or Pa barometric pressure units • Select barometric pressure Instructions on page 21. OFS: % saturation offset adjustment • Set % saturation offset adjustment Instructions on page 24. CAL: Viewing previous calibration data • View previous calibration data, including date/time Instructions on page 25.
Instruction Manual 8.2 DO 300 Clr: Memory Clear Use this feature to clear all memory values when you need to store a new series of values. This will avoid confusing the old values with the new ones. From measurement mode: 1. 2. Press the SETUP key to enter SETUP mode. Press MI/ or MR/ keys to scroll through main groups until you view parameter “CLr” in the upper display. 3. 4. Press the ENTER key to enter parameter. Press MI/ or MR/ keys to toggle between NO and YES. 5.
Instruction Manual 8.3 DO 300 dPr: Dissolved Oxygen Parameters This parameter lets you adjust the barometric pressure and salinity. 8.3.1 Pressure adjustment mode Barometric pressure is vital to correct dissolved oxygen measurements. You need to enter the correct barometric pressure of the area where you are performing your DO measurement. This mode lets you perform two functions: Select either mm Hg or Kilo Pascal barometric pressure units. Adjust the barometric pressure.
Instruction Manual DO 300 Figure 18: Change pressure units (mm Hg or kPa) & adjusting its values 22
Instruction Manual 8.3.2 DO 300 Salinity Adjustment Mode NOTE: This mode appears only from mg/L (ppm) SETUP mode . Salinity correction mode lets you correct for the variations in oxygen solubility due to salt concentration in the sample. From measurement mode: 1. Press the MODE key to select mg/L (ppm) mode. 2. Press the SETUP key to enter SETUP mode. 3. Press MI/ or MR/ keys to scroll through main groups until you view parameter “dPr” in the upper display. 4. Press the ENTER key five times.
Instruction Manual 8.4 DO 300 OFS: Offset for % Saturation Measurement NOTE: This feature appears from % saturation SETUP mode only. From measurement mode: 1. Press the MODE key to select % saturation mode. 2. Press the SETUP key to enter SETUP mode. 3. Press MI/ or MR/ keys to scroll through main groups until you view parameter “OFS” in the upper display. 4. Press the ENTER key. The upper display shows the current measurement in % saturation and the lower shows “OFS”. 5.
Instruction Manual 8.5 DO 300 CAL: Previous Calibration Information This feature shows you the previous calibration data, along with date and time of calibration. This is a “view only” parameter. In % saturation SETUP mode: Calibration information is shown in % saturation units. In mg/L (ppm) SETUP mode: Calibration information is shown in mg/L (ppm) units. From measurement mode: 1. Press the MODE key to select the calibration data you want to view: % or mg/L (ppm). 2. 3.
Instruction Manual 8.6 DO 300 ELE: Electrode Properties These “view only” parameters show you the electrode properties for diagnostic purposes: A. Checking electrode data from % saturation SETUP mode Probe Slope: Lets you view and give an indication of the probe’s efficiency. The value displayed is the ratio of the SETUP theoretical value to the actual value produced by the probe. ele The higher the number, the lesser output from the probe. The p 5.0 ratio displays from 0.5 to 1.999. B.
Instruction Manual 6. DO 300 Press the ENTER key. The upper display shows the 100% saturation mV value. 7. Checking electrode data from mg/L (ppm) SETUP mode Press the ENTER key. The upper display shows the 0% saturation mV value. SETUP 8. Press the ENTER key to move back to main group “ELE”. If ele you want to return to measurement mode, press CAL/MEAS. p 4.0 ER ENT SETUP K= 1.000 mg/l el 1 ER ENT SETUP mV 39.2 EL 2 ER ENT SETUP - 0.
Instruction Manual 8.7 DO 300 COF: Unit Configuration Unit configuration mode lets you select the following parameters: A. READY indicator and auto endpoint function. B. mg/L or ppm units (available from mg/L or ppm SETUP mode only) C. Temperature in °C or °F.
Instruction Manual 8.7.1 DO 300 READY Indicator and Auto Endpoint Function The first program lets you select: • “READY indicator ON” to indicate when the reading is stable. • “READY indicator OFF” for faster meter response. • Auto Endpoint Function ON. Select auto endpoint ON to “HOLD” the reading when it is stable for more than 5 seconds. The display automatically freezes, and the HOLD indicator appears on the left side of the display.
Instruction Manual 8.7.2 DO 300 Selecting mg/L or ppm units NOTE: This mode appears in mg/L (ppm) SETUP mode only. This mode lets you have the option to select either mg/L or ppm dissolved oxygen units. From measurement mode 1. Press the MODE key to select mg/L (ppm) mode. 2. Press SETUP key to enter SETUP mode. 3. Press MI/ or MR/ keys to scroll through main groups until you view parameter “COF” in the upper display. 4. Press the ENTER key until “dO” appears in the upper display. 5.
Instruction Manual 8.7.3 DO 300 Selecting °C or °F Temperature Unit This meter lets you select between °C and °F units for temperature readings. From measurement mode 1. 2. Press SETUP key to enter SETUP mode. Press MI/ or MR/ keys to scroll through main groups until you view parameter “COF” in the upper display. 3. Press the ENTER key until “C” or “F” appears in the upper display. 4. Press MI/ or MR/ keys to toggle between °C and °F. 5.
Instruction Manual 8.8 DO 300 LCd: Adjusting LCD Brightness This mode allows you to make adjustment to the brightness of the backlit LCD. Selecting a dimmer back-lighting level helps conserve batteries. From measurement mode 1. 2. Press SETUP key to enter SETUP mode. Press MI/ or MR/ keys to scroll through main groups until you view parameter “LCd” in the upper display. 3. Press the ENTER key. A number (0 – 8) appears in the upper display and “LCd” appears in the lower display. 4.
Instruction Manual 8.9 DO 300 CLO: Setting the Real-Time Clock Your meter features a real-time calendar and clock. This helps you meet GLP (Good Laboratory Practice) standards. From measurement mode 1. 2. Press SETUP key to enter SETUP mode. Press MI/ or MR/ keys to scroll through main groups until you view parameter “CLO” in the upper display. 3. Press the ENTER key to enter parameter “CLO”. The meter lets you select the century: “19” or “20”. The century digits will flash. 4.
Instruction Manual 14. Press MI/ DO 300 or MR/ keys to toggle to the correct minutes. 15. Press the ENTER key to confirm the minutes and move to “second” selection. The “second” digits will flash. 16. Press MI/ or MR/ keys to toggle to the correct seconds. 17. Press the ENTER key to confirm selection and to return to the main group menu. Press the CAL/meas key to return to measurement mode. NOTES Press the CAL/MEAS key at any point while setting the time to return to the main group menu.
Instruction Manual 8.10 DO 300 rSt: Resetting to Factory Default Setting This program lets you reset all parameters to factory default settings. This clears all calibration data, memory, and any other setup functions you might have changed. However, the clock settings will remain as you have set them. There are also some other parameters that retain settings when reset is done. See Factory Default Setting on page 51 for a table of factory default condition. From measurement mode 1. 2.
Instruction Manual 9 DO 300 PROBE CARE AND MAINTENANCE The probe is a galvanic measuring element which produces an output proportional to the oxygen present in the medium in which it is placed. The galvanic probe design lets you take measurements immediately – without the typical 15 minute wait of other dissolved oxygen probes. The probe consists of two parts: • An upper part consisting of an anode, a cathode, and cable. • A lower part consisting of a membrane cap, membrane, and electrolyte solution.
Instruction Manual 9.1 DO 300 Probe Care Under typical operating conditions, the probe should last for several years. Proper care and maintenance will help you receive the maximum probe life and ensure more accurate readings. Since any deposits on the membrane surface act as a barrier to oxygen diffusing through the membrane, the membrane must be cleaned at regular intervals to assure maximum reliability.
Instruction Manual 9.2 DO 300 Membrane Housing Replacement Replacement of the membrane cap housing/ membrane is required only when you cannot calibrate the probe, or if the membrane is damaged. Typical membrane damages are punctures or wrinkles caused during measurements or cleaning. Your new DO probe comes with a replacement membrane housing. To order more replacement membrane housing , see the “Accessories” section on page 47 - 48. 9.2.
Instruction Manual 9.3 DO 300 Membrane/O-ring Replacement (Optional Procedure) It is recommended only experienced service personnel can perform this procedure. This procedure is OPTIONAL, and should only be performed if you have new membrane and O-ring. You are also required to have a membrane installation tool. These items are available as optional accessories in the Accessories section. 1. Pull off the probe guard. See figure on right. 2. Unscrew the membrane cap from the probe. 3.
Instruction Manual DO 300 10. Using the installation tool, screw the membrane lock back into the cap. Tighten the lock firmly over Installation tool the membrane and O-ring, but do not over tighten. Close Open 11. Inspect the membrane for wrinkles. If wrinkles exist, remove the membrane and repeat steps 8 – 11. 12. Fill the membrane cap with water and inspect the bottom for leaks.
Instruction Manual 9.4 DO 300 Electrolyte Solution The electrolyte solution in your probe’s cap will periodically evaporate and need to be replaced. Your new DO probe comes with accessories of one 50-ml replacement electrolyte solution and a spare membrane cap. The replacement electrolyte comes premixed and ready to use. To order more electrolyte solution, see Accessories section.
Instruction Manual 9.5 DO 300 DO Probe Troubleshooting Table When experiencing difficulties with the equipment, keep in mind of the following: 1. Check for the obvious, such as physical condition of the probe, any signs of damage to the cable, power and signal connections etc. 2. Determine whether it is the probe, meter or the surrounding environment that is causing the problem. The following troubleshooting table identifies most of the problems likely to occur: Problem 1.
Instruction Manual Problem 3. It is not possible to calibrate the probe in air – the display will not read high enough after fully adjusting the offset. DO 300 Probable Causes Probe has dried out – no electrolyte inside. Probe is overdue for servicing – excessive build-up of anode oxide. A deposit has built-up on the silver cathode, which is inhibiting the reduction of oxygen at its surface. Solution Service probe and change membrane. Use a stiff nylon brush to remove the oxide built-up from the anode.
Instruction Manual 10 DO 300 TROUBLE SHOOTING GUIDE Problem Cause Solution No display when turned on a) Batteries not in place a) Check that batteries are in place and making good contact. b) Batteries not in correct polarity (+ and – position). b) Re-insert batteries with correct polarity. c) Weak batteries c) Replace batteries. a) Insufficient electrolyte in probe. a) Fill probe with electrolyte & replace membrane. See page x. b) Air bubbles trapped around the probe.
Instruction Manual 11 DO 300 ERROR MESSAGES LCD Display Indicates Cause Solution Err annunciator Unrecognised input from keypad Wrong input in selected mode. Release key. Select valid operations depending on mode. CAL & Err annunciators on / Buffer and electrode indicators blink. Calibration error. Wrong value input at calibration. Check your input value, clean probe. Dirty probe. See Calibration sections or Probe Maintenance section. Battery indicator blinks Low battery level.
Instruction Manual 12 DO 300 SPECIFICATIONS Dissolved Oxygen Range Resolution Relative accuracy 0.00 – 19.99 mg/L or ppm 0.01 mg/L; 0.01 ppm ± 1.5% of Full Scale % Saturation of Oxygen Range Resolution Relative accuracy 0.0 – 199.9 % 0.1 % ± 1.5% of Full Scale Temperature Range Resolution Relative accuracy 0.0 – 50.0 °C (32.0 – 122.0 °F) 0.1 °C (0.1 °F) ± 0.3 °C (0.5 °F) Salinity Correction Range Resolution Method 0.0 – 50.0 ppt 0.
Instruction Manual 13 DO 300 ACCESSORIES Replacement Meter and Meter accessories Eutech Instruments Item Eutech Instruments Ordering Code. Waterproof DO 300 Dissolved Oxygen meter (back-lit display) complete with submersible DO electrode (3m/ 10ft cable), electrode maintenance kit and carrying case with accessories EC-DOWP300/01K Waterproof DO 300 Dissolved Oxygen meter (back-lit display) complete with submersible DO electrode (25ft cable) EC-DOWP300/02 Maintenance kit for DO electrode.
Instruction Manual DO 300 Oakton Instruments Item Oakton Instruments Ordering Code. Waterproof DO 300 Dissolved Oxygen meter with submersible DO electrode (3m/ 10ft cable), electrode maintenance kit, and carrying case with accessories 35641-00 Waterproof DO 300 Dissolved Oxygen meter only. 35640-01 Replacement submersible Dissolved Oxygen probe with 3-meter (10-ft) cable.
Instruction Manual 14 DO 300 DISSOLVED OXYGEN & METER THEORY Dissolved Oxygen (DO) refers to the volume of oxygen that is contained in water. There are two main sources of DO in water: from atmosphere and photosynthesis. Waves and tumbling water mix air into the water where oxygen readily dissolves until saturation occurs. Oxygen is also produced by aquatic plants and algae as a by-product of photosynthesis.
Instruction Manual 15 DO 300 PRESSURE VS ALTITUDE TABLE Barometric pressure affects DO readings; therefore this meter lets you enter the correct barometric pressure at your altitude. If you do not have equipment that lets you measure the exact barometric pressure at your altitude, you can estimate it using the chart below.
Instruction Manual 16 DO 300 FACTORY DEFAULT SETTINGS Resetting the meter to factory default settings clears all DO calibration data and memory, and returns other setup functions to the default settings shown in the table below. However, the clock settings will remain as you have set them. See page 35 for directions on setting the meter to factory default settings.
17 OVER-VIEW OF SETUP FUNCTIONS (The values shown above are for illustration purpose.
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Instruction Manual 18 DO 300 WARRANTY This meter is supplied with a three -year meter warranty and six-month pH/ DO probe warranty, against significant deviations in material and workmanship. If repair or adjustment is necessary and has not been the result of abuse or misuse within the designated period, please return – freight pre-paid – and correction will be made without charge. Eutech Instruments/ Oakton Instruments will determine if the product problem is due to deviations or customer misuse.
For more information on Eutech Instruments/ Oakton Instruments’ products, contact your nearest distributor or visit our website listed below: Oakton Instruments P.O Box 5136, Vernon Hills, IL 60061, USA Tel: (1) 888-462-5266 Fax: (1) 847-247-2984 E-mail: info@4oakton.com Web-site: www.4oakton.com www.oaktoninstruments.com Eutech Instruments Pte Ltd. Blk 55, Ayer Rajah Crescent, #04-16/24 Singapore 139949 Tel: (65) 6778 6876 Fax: (65) 6773 0836 E-mail: marketing@eutechinst.com Web-site: www.eutechinst.
