Specifications

• Graphic LED display.
Gel imaging system
• System with true 16 bit CCD with more than 4 Megapixel image resolution.
• Cooled CCD with cooling range of -30°C absolute temperature using peltier cooling system. (if any other technology
used, please quote its superiority)
• System with dynamic flat fielding technology for getting accurate Quantitation with CV < 5 %.
• Multi image color target with multi-channel image display for study of more than 2 dyes at a time for differential
expression. Automatic sequential imaging of multi labeled samples.
• System should have 8 different modes of illumination – Trans-UV, epi-white, no illumination for chemiluminescence,
trans-white, optional epi-red, optional epi-green, optional epi-blue, and optional trans-blue. All LEDs should be
integrated with system for proper imaging of multi target with reproducibility.
• Instrument should have a large transillumination area of which is capable of taking sample of minimum 28x36 cm and
should have a minimum imaging area of 26 x 35 cm.
• Capability of stain free gel imaging is essential to avoid time consuming gel/sample staining procedure.
• Instrument should have auto focus technology that is system should automatically take the best focus depending on
any zoom level without the movement of sample platform. The sample platform should be fixed at one position to
ensure minimal mechanical movement.
• Pixel size should be 6.45 µm x 6.45 µm or bigger.
• Instrument should have a minimum 6 position filter wheel for capturing images of various dyes effectively. The filter
wheel should be motorized and automatic without the requirement of manually changing or moving the filter wheel.
• Dynamic range should be >4 orders of magnitude for good quantification.
• Pixel density should be at least 65,535.
• System should offer various binning options with minimum of 2x2, 3x3 and 4x4 for customized sensitivity.
Software for Image acquisition and 1-Dimensional Analysis:
Suitable software compatible with the apparatus to enable all the functions ( please quote all salient features)
2-D Gel Analysis software
:
• Gaussian modeling based software
• Provision for available algorithms for Automatic Spot Detection & Quantification.
• Spot detection summary matching summary, replicate group consensus tool to optimize spot
detection and matching parameter
• Provision for auto recognition and removal of background speckles.
• Possibility of simultaneous analysis of up to 15 gels
• Compatibility for XML data and JPEG file format
• Network license support for more number of users.