Specifications

F. No. 120/Pharma/RPC/SSK/12-13
2D Gel Electrophoresis with imaging System – 01 No.
Specifications
Complete system to separate, quantitate, characterize, visualize and analyze proteins in biological samples. System
should have following features:
A) Isoelectric focusing unit (1
st
Dimension):
• System should include 12 different Individual Lane Control for running different samples, pH Gradients and focusing
protocols in a single run with different protocols.
• Software Interface for creating and editing protocols and setting up the program rapidly.
• Provision to interface with website for online data interpretation.
• Must provide flexibility to run IPG strips gel side up, gel side down and with cup loading configuration.
• System should have voltage 0–10,000 V, 1 V increments(50-10000V) or better
• Current range should be 0–100 µA per lane at 1 µA intervals or better
• System should have peltier based cooling platform.
• Temperature range should be 10–25ºC ±1.0ºC @ max ambient 23ºC and 18–25ºC ±1.0ºC @ max ambient 31ºC.
• Focusing trays should be made of polycarbonate for contamination free process.
• System should be capable of simultaneously running minimum 12 IPG strips of various lengths like 7, 11, 13, 17, 18, and
24 cm.
• System should have ramping Step, linear, gradual, and hold voltage ramping for each focusing step. Hold mode as a final
step to prevent diffusion when IEF is complete.
State-of the art Computer (if required to run the software) along with laser printer with UPS must be quoted along with the
system
B) SDS-PAGE Electrophoresis unit (2
nd
Dimension):
For Mini gels
• (7 cm) capable of running 2 mini gels simultaneously
• Capable of running hand cast as well as precast gel.
• Separate running and casting module
• Capable of using blotting module to do western blotting.
• Leak proof, tape free and easy to assemble
• Wet transfer blotting module of gel size should be provided
For Large Gels
• Versatile system format to perform SDS, 1D, 2-D and Native gels
• SDS-PAGE apparatus to run 11-13 cm gels and wet blotting system with features mentioned below.
• Capable of running IPG strips
• Provision to enable uniform pressure along entire length of the glass plates which is expected to provide leak proof
seal without tapes, grease or agarose.
• Choice of glass plates, spacers & sandwich clamps to cast 2 gels.
• Blotting module of similar gel size should be provided
Power supply
• Output range (programmable) – 10-500 V, fully adjustable in 1 V steps and 0.01-2.5 A, fully adjustable in 1 mA
steps, 1-400 W power.
• Type of output – Constant voltage, constant current, constant power or constant temperature.
• Output terminals – 4 pair recessed banana jacks in parallel
• Safety features - No-load detection, sudden load change detection, overload/short-circuit detection, over voltage
protection
• Timer - 1 min–99 hr 59 min, fully adjustable