Specifications

(e). Sensitivity : ≤ 1 pM fluorescein in 96 wells < 1.5 pM in 384 wells.
5. Time Resolved Fluorescence
(a). Reading capabilities : Top or Bottom of a Microplate
(b). Wavelength range : 250–850nm
(c). Wavelength selection : Monochromators, tunable in 1nm increments
(d). Bandwidth (EX,EM) : 9nm, 15nm
(e). Precision Data Collection : 1–100 flashes, delay of 0-600 µ.sec before read,
integration time selectable between 50 -1500 µ.sec.
(f). Sensitivity : 10 fM europium in 96 or 384 wells with top-read.
5. Fluorescence Polarization Performance :
(a). Wavelength range : 300 – 750nm
(b). Wavelength Selection : Monochromators, tunable in 1nm increments
(c). Bandwidth (EX, EM) : 9nm, 15nm
(d). Precision : < 5 mP Standard Deviation at1nm Fluorescein in 96 and 384 wells
6. HTRF : System should be Certified for CIS Bio HTRF Assays
7. Luminescence Performance
(a). Reading capabilities : Cuvette, Top and Bottom of a Microplate
(b). Wavelength range : 250 – 850nm
(c). Wavelength selection : all wavelength or with selected Wavelengths
(d). Sensitivity : ≤ 43 pM ATP in 96 wells
(e). Cross-talk : < 0.3 % in white 96- and 384-well Microplate
8. System should have in-built triple mode cuvette ports for Absorbance, FI and Luminescence, in a single system.
9. System should be capable of measuring end-points, kinetics, spectrum scanning, well scanning for all the modes.
10. System should have AutoPMT settings that adjust the optimum voltage for each well’s sample concentration and then
normalizes the raw data to show relative RFU values.
11. System should have temperature-independent Path-Check Capability to normalize the results of the wells of the plate
as that of cuvette.
12. Data Acquisition : Software integrated system which provides data acquisition, analysis and management
capabilities, allowing cross-plate analysis and custom calculations.
Software should have 120 in-built protocols.
13. Suitable branded computer with all accessories
14. Onsite application training of all project staff and continuous technical support from qualified application scientist &
service engineers should be provided by vendor.
Instructions:
¾ The selection committee reserves the rights to select the instrument based upon the suitability and sensitivity
applicable for the utility for which this instrument would be purchased based on the performance details provided.
¾ Vendors are instructed to follow the exact sequence of items as listed/ mentioned in the tender specification sheet/s for
quoting their tender. The quoted tender for technical bid and price bid will be summarily rejected, if the quoted
specification compliance sheet/s not found according to the sequence of the items as listed in the tender specification.
¾ Please quote only for New Branded machine/s. If it will be find during any period of time that
Refurbished/Repaired/Second Hand instruments has supplied and installed instead of new branded machines, an
immediate action will be taken against the distributor/ manufacture as per the rules and regulations of AIIMS. And