Specifications

F. No. 97/Bio/RPC/SSK/12-13
Multimode Microplate Reader – 01 No
1. System should have a Dual Monochromator based Multi detection microplate reader with five following detection
modes:
(a). Fluorescence Intensity (Top & Bottom)
(b). TRF
(c). HTRF
(d). UV-Vis Absorbance
(e). Fluorescence’s Polarization
(f). Glow Luminescence detection
2. General Photometric Performance
(a). Plate formats : 6, 12, 24, 48, 96, 384 wells and Cuvette Ports for Abs, FI
Luminescence Mode
(b). Light Source : Xenon Flash Lamp (1joule / flash)
(c). Detector : 2 Photomultiplier tubes or better
(d). Read time (i) 96-well : Abs-18, FI-17, FP- 42, TRF-17 & Lum- 2 Sec
(ii) 384-well : Abs-49, FI-48, FP-2:03, TRF-48 & Lum-7 Sec
(e). Shaker Time : 0 to 999 seconds
(f). Temp Control : 2
0
C above ambient to 60
0
C
(g). Temp Uniformity : < 1
0
C at 37
0
C set point
(h). Temp Accuracy : ± 1 Deg C at 37 Deg C set point,
(i). Reading Modes : End Point reading / Kinetic reading / Spectral, Scanning for all modes
(j). Well Scanning : Abs, FI, TRF, Lum
3. Absorbance Photometric Performance
(a). Reading Capabilities : Cuvette and Microplate
(b). Wavelength range : 200-1000nm
(c). Wavelength selection : Monochromator, tunable in 1nm increments
(d). Wavelength bandwidth : ≤4.0nm
(e). Wavelength accuracy : ± 2.0nm
(f). Wavelength repeatability : ± 0.2nm
(g). Photometric range : 0 to 4.0 OD
(h). Photometric Resolution : 0.001 OD
(i). Photometric Accuracy (Microplate) : < ± 0.006 OD ± 1.0 %, 0-2OD
(j). Photometric Accuracy (Cuvette) : < ± 0.005 OD ± 1.0 % , 0-2 OD
(k). Photometric precision : < ± 0.003 OD ± 1.0 %,0-2 OD
4. Fluorescence Intensity Top and Bottom Reading Performance :
(a). Reading capabilities : Cuvette or top or bottom of a Microplate
(b). Wavelength range : 250 – 850nm
(c). Wavelength Selection : Monochromators, tunable in 1nm increments
(d). Bandwidth (EX, EM) : 9 nm, 15 nm