User`s manual

3-10
3
Press
STEP
to accept all of the default values and move to the next
display.
The display for Setpoint #2 appears:
This display sets the ramp time, temperature, and hold time for
Setpoint #2 — used for primer annealing.
For a three-temperature cycle, the second PCR setpoint is
commonly 55 degrees C and is held for 30 seconds.
4
Press
STEP
to accept the default ramp time, temperature, and hold
time for Setpoint #2.
The display for Setpoint #3 appears:
This display sets the ramp time, temperature, and hold time for
Setpoint #3 — used for primer extension.
For a three-temperature cycle, the third PCR setpoint is commonly
72 degrees C and is held for 30 seconds.
5
Press
STEP
to accept the default ramp time, temperature, and hold
time for Setpoint #3.
Setpt #2 Ramp 0:00
55.0C Hold 0:30
Setpt #3 Ramp 0:00
72.0C Hold 0:30