User`s manual

3-3
What is a CYCL Program?
A CYCL program is a set of instructions in which you specify how
the instrument should heat and cool your samples in a PCR
cycle. The CYCL program is stored in the instrument software.
You view the CYCL program by scrolling through a series of
displays on the front panel screen. By entering the times and
temperatures for your PCR application, you can easily customize
the program to your needs.
The CYCL program is a common type of PCR thermal profile.
Typically, it consists of two or three
setpoints or incubation
temperatures (see Figure 3-1). The first setpoint is used for
template denaturation or melting. The second setpoint is used for
primer annealing, and the third setpoint is used for primer
extension.
Each cycle may be repeated up to 99 times.
Figure 3-1
A common CYCL program.