User`s manual

9
Note
If methods developed on the GeneAmp PCR System 9600 are
to be used on the DNA Thermal Cycler, you can use Table 1 to convert
the hold times from GeneAmp PCR System 9600 to DNA Thermal
Cycler. Simply add the indicated times instead of subtracting them.
For down ramps, determine the change in temperature required
to reach the next target temperature, then round off this value to
the closest value given below. Then, based on the starting
temperature of the ramp, determine the number of seconds to
subtract from the DNA Thermal Cycler hold time to arrive at the
new hold time. The typically recommended hold time is 10 to 15
seconds for annealing.
Note
If methods developed on the GeneAmp PCR System 9600 are
to be used on the DNA Thermal Cycler, you can use Table 2 to convert
the hold times from GeneAmp PCR System 9600 to DNA Thermal
Cycler by adding the indicated times instead of subtracting them.
Table 2. After Down Ramps: Converting Hold Times Greater than
One Minute (DNA Thermal Cycler to GeneAmp PCR System 9600)
If your change in
temperature (°C) is:
Subtract this time from the DNA
Thermal Cycler hold time if the starting
temperature of the ramp is:
35-55 ° 56-75 ° 76-95 °
10 ° 39 sec. 39 sec. 40 sec.
15 ° 45 sec. 47 sec. 48 sec.
20 ° 49 sec. 52 sec. 53 sec.
30 ° 56 sec. 59 sec.
40 ° 57 sec. 62 sec.
50 ° 62 sec.
60 ° 60 sec.