User`s manual

8
Tables 1 and 2 show some guidelines for converting hold times
for the DNA Thermal Cycler or DNA Thermal Cycler 480 to hold
times for the GeneAmp PCR System 9600. The table you use will
depend on whether you are using an
up ramp (the temperature is
higher than that used in the previous segment) or a
down ramp
(the temperature is lower).
♦
If you are using an
up ramp, you will base the new hold time
on the
change in temperature
required to reach the next
target temperature. See Table 1.
♦
If you are using a
down ramp, you will base the new hold time
on the
starting temperature of the ramp and the change in
temperature
required to reach the next target temperature.
See Table 2.
For up ramps, determine the change in temperature required to
reach the next target temperature, then round off this value to the
closest value given below. Then subtract the number of seconds
indicated from the hold time used for the DNA Thermal Cycler.
The result is the hold time to use for the GeneAmp PCR System
9600. The typically recommended hold time is 10 to 15 seconds
for denaturation.
Table 1. After Up Ramps: Converting Hold Times Greater than
One Minute (DNA Thermal Cycler to GeneAmp PCR System 9600)
If your change in
temperature (°C) is:
Subtract this time from your
Thermal Cycler hold time:
10 ° 38 sec.
15 ° 45 sec.
20 ° 49 sec.
30 ° 54 sec.
40 ° 55 sec.
50 ° 57 sec.
60 ° 57 sec.