User`s manual

3
Comparing Files and Programs
In the GeneAmp PCR System 9600 software, PCR files are
referred to as
programs . The following table lists the differences
between DNA Thermal Cycler PCR files and GeneAmp PCR
System 9600 PCR programs.
Function File on the DNA
Thermal Cycler
Program on the GeneAmp
PCR System 9600
PCR
INCUBATIONS
SOAK (File #1): An
incubation file.
TIME DELAY (File #2):
An incubation file.
HOLD: Set the hold time to the
FOREVER option for an
indefinite soak.
HOLD: Set the hold time to a
pre-defined time.
PCR CYCLING THERMO-CYCLE
(File #3): Use the
default value (0:01) for
the fastest possible
ramp time.
STEP-CYCLE (File #4):
You cannot program
ramp times; target
temperatures are
reached as quickly as
possible.
CYCL: Program ramp times
and hold times as desired for
each setpoint.
CYCL: Use the default value
(0:00) for the fastest possible
ramp time.
AUTO-SEGMENT
EXTENSION
THERMO-CYCLE
or STEP-CYCLE with
auto-segment extension
AUTO: This program can
automatically increase or
decrease the setpoint hold time
and setpoint temperature in
successive cycles.
METHODS Link each file to another
file using the “link to
stored file #” parameter
within each file.
METH: Use this program to
link together a series of
programs.