User`s manual

8-11
♦
If the block average is more than 0.75 degree C above or
below 95 degrees C, your instrument must be recalibrated.
For example:
If the measured temperature of well E1 at was 95.2°C, and the High
Offset printed on your calibration label is –0.1, then you would use
the following formula:
In this example, since 95.3°C does not differ by +/–0.75°C from
your programmed target temperature, your instrument would not
need to be recalibrated.
Note
The
offset
is the number of degrees Celsius that the
temperature of well E1 differed from the average temperature of the
block when the instrument was calibrated at the factory.
2
Use the following formula to calculate the average block
temperature at the 40-degree C hold:
Block Average at 40 °C = T(40) – Low Offset
♦
If the block average is more than 0.75 degree C above or
below 40 degrees C, your GeneAmp PCR System 9600
needs to be recalibrated.
For example:
If the measured temperature of well E1 was 39.9°C, and the Low
Offset printed on your calibration label is +0.1, then you would use
the following formula for computing the block average at 40°C:
In this example, since 39.8
°
C does not differ by +/-0.75
°
C from your
programmed target temperature, your instrument would not need to
be recalibrated.
3
Remove the probe assembly from the sample block and move the
digital thermometer ON-OFF/RANGE switch to the OFF position.
Block Average at 95°C = 95.2 – (–0.1)
= 95.3°C
Block Average at 40°C = 39.9 – (+0.1)
= 39.8°C