User`s manual

5-34
Description of CYCL Program Displays
Defines the number of incubation temperatures or
setpoints used in the PCR cycle. A typical PCR
cycle consists of three (3) setpoints; therefore, #3 is
the default value.
Press ENTER to accept the default, or type a
different number (1-9), then press ENTER.
Setpoint #1 in a typical cycle program is used for
DNA denaturation or melting. This display sets the
following values for the first setpoint (Setpt #1):
♦
Setpoint temperature. The default value is 94.0
degrees C.
♦
Ramp time: the time taken to change from one
temperature to another. The default value (0
minutes, 0 seconds) causes the sample to heat
or cool to the first setpoint as fast as possible.
♦
Hold time: the length of time the samples will be
maintained at the specified temperature. The
default value is 30 seconds.
Note
If you previously used the DNA Thermal Cycler,
note that you will be using shorter hold times for the
GeneAmp PCR System 9600. Refer to page 5-2 for more
information.
3 Temperature PCR
Setpt #1 Ramp 0:00
94.0C Hold 0:30