User`s manual

5-6
Table 5-2 After Down Ramps: Converting Hold Times Greater
than One Minute (DNA Thermal Cycler to GeneAmp PCR System
9600)
For down ramps, determine the change in temperature required
to reach the next target temperature, then round off this value to
the closest value given below. Then, based on the starting
temperature of the ramp, determine the number of seconds to
subtract from the DNA Thermal Cycler hold time to arrive at the
new hold time
.
The typically recommended hold time is 10 to 15
seconds for annealing.
Seconds to Subtract from DNA Thermal Cycler Hold Times (> 1 min.)
Figure 5-3 shows an example of converted hold times
∆
Temp. Starting Temperature of Ramp
(°C)
(°C)
35-55°
56-75° 76-95°
10° 39 sec. 39 sec. 40 sec.
15° 45 sec. 47 sec. 48 sec.
20° 49 sec. 52 sec. 53 sec.
30° 56 sec. 59 sec.
40° 57 sec. 62 sec.
50° 62 sec.
60° 60 sec.