User`s manual

5-5
Figure 5-2 shows an example of converted hold times.
In this example, the temperature was increased by 17 degrees C,
This value was rounded to 15 degrees C. According to Table 5-1,
we should subtract 45 seconds from the hold time on the DNA
Thermal Cycler, giving us a new hold time of 35 seconds.
Figure 5-2
Sample conversion of hold times for up ramps (DNA Thermal
Cycler to GeneAmp PCR System 9600)
Note
If methods developed on the GeneAmp PCR System 9600 will
be used on the DNA Thermal Cycler, you can also use Table 5-1 to
convert the hold times. Simply add the indicated times instead of
subtracting them.