User`s manual

4-14
PCR Methods
PCR methods are used to automatically link together up to 17
programs for a specific PCR application. To create a method, you
use the METH program. An example of a method is shown in
Figure 4-8. This method starts with a HOLD file used for pre-PCR
denaturation (e.g., 94.0 degrees C for five minutes). This is
followed by a CYCL program for three-temperature PCR. A final
HOLD program concludes the run, for post-PCR storage
overnight (at 4.0 degrees C, for example).
Figure 4-8
An example of a PCR method.