User`s manual

4-12
The AUTO Program
An AUTO program allows the user to automatically increment or
decrement the setpoint times and temperatures a fixed amount
every cycle.
By increasing the extension time with each cycle, it may be
possible to increase product yield with a fixed amount of enzyme.
The enzyme is not initially limiting, but eventually, if more enzyme
is not added, longer times may be needed to continue with
exponential increases in product.
An AUTO program may also be desirable when it is found that the
melting temperature of DNA is increasing as the DNA becomes
more concentrated. In this instance, an AUTO program would be
used to incrementally increase the denaturation temperature over
a series of cycles.
Another use of an AUTO program is to incrementally increase the
annealing temperature over the first several cycles for greater
specificity of primer binding. After the first several cycles (usually,
two to five), the annealing temperature is kept constant.