on see Instructions Qualified Eppendorf® p. Tab. Fig. —p. for UVette® use use Instructions for 0012 546.
Copyright ©2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. eppendorf®, Eppendorf®, and UVette® are registered trademarks of Eppendorf AG, Hamburg, Germany. Trademarks are not marked in all cases with ™ or ® in this manual. U.S. Patent No. 6,249,345 0012 546.
Eppendorf® UVette® — Instructions for use 1 1.1 1 Operating instructions Using this manual Operating instructions Before using the consumables for the first time, read these instructions for use and the operating manual of the device that you use the consumables with. You can find the current version of the operating manual on the Internet at www.eppendorf.com/ worldwide. These instructions for use do not replace the device operating manual. 2 Product description 2.
Eppendorf® UVette® — Instructions for use 2.2 Sales units UVette (individually packaged) UVette routine pack Starter set 2.
Eppendorf® UVette® — Instructions for use 3 Safety 3.1 Intended use 3 Safety The Eppendorf UVette is a UV-transparent plastic cuvette for single use. It is used for photometric analysis of liquids in the UV range and the entire VIS range. The Eppendorf UVette is used in training, routine and research laboratories, and in the life sciences, industry, and chemistry. In-vivo applications (in or on the human body) are not permitted.
Eppendorf® UVette® — Instructions for use 4.1.1 Eppendorf BioPhotometer/Eppendorf BioSpectrometer 1. Position the cuvette so the required optical path length points toward the light path. 2. During insertion, press the cuvette all the way down against a light resistance. 4.1.2 Inserting the cuvette and adapter in photometers from other manufacturers Function of the adapter • Adjust the height of the light beam in the corresponding photometer.
Eppendorf® UVette® — Instructions for use Limited tempering efficiency! The tempering efficiency is limited by the contour of the UVette. • If temperature control is required, check to see if the UVette is suitable for your applications. Hint! 1. Set the parameter for the cuvette layer thickness in the device. 2. Determine the blank value of the cuvette. Abb. 2:Absorption of the water-filled UVette Absorbance 0. 0.5 0.1 200 300 400 Wavelength (nm) Fig. 2: Absorption of the water-filled UVette 3.
Eppendorf® UVette® — Instructions for use 5 Troubleshooting 5.1 5 Error search Troubleshooting Symptom/ message Measured values are imprecise. Cause • The blank value and sample • • • Measured values are not plausible. • • • 8 Remedy Measure the blank value and value have been measured sample value in the same in different cuvettes. UVette. The position of the UVette in Press the UVette firmly onto the device is not correct. the base of the cuvette shaft or adapter.
Eppendorf® UVette® — Instructions for use 6 Technical data Dimensions Width Depth Height Filling volume 6 Technical data Photometric properties Optical path length Light path height Wavelength range Cuvette blank Cuvette blank scattering 12.5 mm (0.49 in) 12.5 mm (0.49 in) 36.0 mm (1.42 in) 50 μL – 2 000 μL 2 mm, 10 mm 8.5 mm 220 nm – 1 600 nm 0.5 A at 260 nm (absorbance of the water-filled cuvette against air) s 0.
Eppendorf® UVette® — Instructions for use 7 Ordering Information 7 Ordering Information Order No. (International) Order No. (North America) Description 952010051 UVette 220 nm – 1 600 nm Original Eppendorf consumable, PCR clean, Protein-free 80 cuvettes, individually packaged 0030 106.318 952010069 UVette routine pack 220 nm – 1 600 nm Eppendorf Quality 200 cuvettes, reclosable box 4308 078.
Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt.