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Copyright© 2013 Eppendorf AG, Hamburg. Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. TransferMan® 4r, FemtoJet® and Eppendorf PiezoXpert® are registered trademarks of Eppendorf AG, Hamburg, Germany. Registered trademarks are not marked in all cases with ™ or ® in this manual. No part of this publication may be reproduced without the prior permission of the copyright owner. 5193 900.
Table of contents TransferMan® 4r 3 English (EN) Table of contents Operating TransferMan® on see p. Tab. Fig. p. manual 4r English Operating (EN) manual 1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2.
Table of contents 4 TransferMan® 4r English (EN) 4.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.1 Mounting the Z-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.2 Mounting the Y-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.3 Mounting the X-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.4 Mounting the angle head . . . . . . . . . . . . . . . . . . . . . . . .
Table of contents TransferMan® 4r 5 English (EN) 6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1 Switching the device on or off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1.2 Switching the device off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.
Table of contents 6 TransferMan® 4r English (EN) 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 Home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.13.1 Home menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.13.2 Setting the parameter for Home . . . . . . . . . . . . . . . . . . . . . . . . . . 6.13.3 Move the capillary out with the home key . . . . . . . . . . . . . . . . . . 6.13.
Table of contents TransferMan® 4r 7 English (EN) 7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1.2 Capillary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1.
Operating instructions 8 TransferMan® 4r English (EN) 1 1.1 Operating instructions Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. This operating manual is part of the product. Thus, it must always be easily accessible. Enclose this operating manual when transferring the device to third parties. If this manual is lost, please request another one.
Product description TransferMan® 4r 9 English (EN) 2 2.1 Product description Delivery package Quantity Description 1 X-module 1 Y-module 1 Z-module 1 YZ-connector 1 Swivel joint 1 Angle head 1 Control board 1 Mains/power cord 1 Cable sheathing 1 Operating manual 2.1.1 Tools Quantity Description 7 Allen key 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm 1 3 mm Allen torque key 1 1.3 mm Allen key 1 Tool bag 2.1.
Product description 10 TransferMan® 4r English (EN) outer (dynamic) movement range. In the inner range, the joystick movement is transferred directly to the capillary. In the outer range, a greater forwards or backwards movement of the joystick results in an acceleration of the capillary movement. The movement ranges enable moving to any position in the working range of the micromanipulator. Proportional movement is suitable for all working techniques that require intuitive, sensitive handling, e.g.
Product description TransferMan® 4r 11 English (EN) 2.3.1 Motor module The motor module is made up of three modules. The capillary can be moved in all three spatial axes due to the layout of the modules. The X-module with the capillary can be swung out of the work area using the swivel joint. The injection angle of the capillary can be set to any angle on the angle head. Abb. 2-2:Motor module 1 4 2 5 6 3 Fig.
Product description 12 TransferMan® 4r English (EN) 2.3.2 Control board The control board contains the key pad, the display and the joystick, and, to the side, the selection dial. The direction of movement and the speed of the joystick are transferred to the capillary. The responsitivity of the movement and the size of the working range are predefined in the software setting. On the control panel, the working range can be selected and individually changed using the selection dial. Abb.
Product description TransferMan® 4r 13 English (EN) Abb. 2-4:Control board - rear 2 1 ext. Device Z-Axis 3 Y-Axis X-Axis SERVICE 8 Fig.
Product description 14 TransferMan® 4r English (EN) 2.4 Control panel With the keys on the control panel you can switch on the control board and select the size of the working range. Via the softkeys you can open the applications, execute the functions, navigate the menu and set the parameters. Abb. 2-5:Control panel 7 6 5 coarse home 1 2 fine x–fine 3 menu 4 Fig.
Product description TransferMan® 4r 15 English (EN) 2.5 Joystick The joystick moves the capillary in all three spatial axes. The joystick movement is transferred directly to the capillary in the proportional range. In the dynamic range, the movement of the capillary is accelerated the further the joystick is moved forwards and backwards. Abb. 2-6:Joystick 1 2 3 Fig. 2-6: Joystick 1 Joystick key 2 2 Swivel Controls movements in the Z-axis Lower part Controls movements in the X and Y-axis Abb.
Product description 16 TransferMan® 4r English (EN) 2.5.1 Proportional range In the proportional range, the capillary will move as quickly or slowly as the joystick is moved. The travel of the capillary is also proportional to the distance that the joystick has been moved forwards and backwards. The movement of the capillary will stop as soon as the joystick is no longer moved or when the position is reached at which the joystick was stopped.
Product description TransferMan® 4r 17 English (EN) 2.5.4 Direction of movement of the rotating wheel The rotating wheel on the joystick moves the motor module in the vertical axis. This activates the motor of the Z-module. Move the capillary in the vertical Abb. 2-9:Movement in the Z-axis direction (Z-axis). Fig. 2-9: Movement in the Z-axis 2.5.5 Joystick key functions Uncouple the joystick from the motor Abb. 2-10:No movement of the capillary module. Trigger functions (e.g.
Product description 18 TransferMan® 4r English (EN) Move capillary over a large distance. Position capillary roughly and quickly. Abb. 2-11:Large working range – Fig. 2-11: Large working range – coarse Move capillary over a medium distance. Position capillary precisely. Abb. 2-12:Medium working range – Fig. 2-12: Medium working range – fine Move capillary over a very short Abb. 2-13:Small working range – distance. Position capillary very precisely and slowly.
Product description TransferMan® 4r 19 English (EN) 2.7 Resulting speed In the inner (proportional) range, the speed of the capillary is dependent on the speed at which the joystick is moved forwards and backwards and the size of the set working range. If the joystick is moved forwards and backwards at the same speed with, e.g., the setting fine or coarse, the resulting speed with the smaller fine working range is less than with the larger coarse range.
Safety 20 TransferMan® 4r English (EN) 3 3.1 Safety Intended use The TransferMan 4r has been designed and manufactured for use in biological, chemical and physical research. It is used for the precise positioning of microcapillaries and similar tools as well as for the transfer of very small sample volumes. The TransferMan 4r has been designed and manufactured for research use only. The TransferMan 4r is intended exclusively for indoor use and for operation by qualified staff. 3.
Safety TransferMan® 4r 21 English (EN) WARNING! Damages to health due to infectious liquids and pathogenic germs. When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes. Wear personal protective equipment.
Installation 22 TransferMan® 4r English (EN) 4 4.1 Installation Preparing installation NOTICE! Damage to the control board as a result of incorrect handling. Grasp the control board on the housing. Do not lift the control board using the joystick. Never place the control board on the joystick. Keep the packaging and the transport securing devices for later transport or storage. Do not operate the device if there is visible damage to the device itself and/or to its packaging. 1. 2. 3. 4.
Installation TransferMan® 4r 23 English (EN) 4.3 Mounting overview Abb. 4-1:Overview for right-hand side mounting 1 2 4 3 10 Fig.
Installation 24 TransferMan® 4r English (EN) 4.3.1 Module (X, Y, Z) Abb. 4-2:Y-module 2 1 3 4 Y 5 Fig.
Installation TransferMan® 4r 25 English (EN) 4.3.2 Z-module holder Abb. 4-3:Z-module holder, front and back 2 1 3 4 Fig. 4-3: Z-module holder, front and back 1 Slider 3 2 Screw Attach Z-module Screw Attach Z-module holder to the adapter 4 Stop angle 4.3.3 Slider Abb. 4-4:Slider 1 Fig.
Installation 26 TransferMan® 4r English (EN) 4.3.4 Angle head Abb. 4-5:Angle head 1 5 Fig.
Installation TransferMan® 4r 27 English (EN) 4.3.5 Swivel joint Abb. 4-6:Swivel joint for right side mounting 5 6 7 1 2 3 8 4 Fig. 4-6: Swivel joint for right side mounting 1 Slider Y-module 2 Turntable 3 Allen screws 4 Slider X-module 4.4 5 Stop plate 6 Upper joint 7 Mounting mark | stands for left side mounting || stands for right side mounting 8 Lower joint Mounting the motor module The motor module can be mounted on the right-hand or left-hand side of the microscope adapter.
Installation 28 TransferMan® 4r English (EN) 4.4.1 Mounting the Z-module Prerequisites • 3 mm Allen torque key 1. Push the Z-module holder onto the microscope adapter and tighten. 2. Push the fixed rail onto the Z-module holder until it reaches the stop angle and tighten the screw. The connecting cable faces to the rear. 4.4.
Installation TransferMan® 4r 29 English (EN) 1. Push the YZ-connector onto the fixed rail of the Y-module and tighten. 2. Push the Y-module with the YZ-connector onto the Z-module and tighten. 4.4.3 Mounting the X-module Prerequisites • 3 mm Allen torque key 1. Push the swivel joint onto the fixed rail of the X-module and tighten.
Installation 30 TransferMan® 4r English (EN) 2. Push the swivel joint with the X-module onto the Y-module and tighten. 4.4.4 Mounting the angle head Prerequisites • 3 mm Allen torque key 1. Push the angle head onto the X-module. 2. Slightly tighten the angle head.
Installation TransferMan® 4r 31 English (EN) 4.5 Inserting the capillary holder into the angle head Prerequisites • A universal capillary holder from Eppendorf is available. • A capillary holder (4 mm diameter) from a different manufacturer is available. 1. Loosen the knurled screw on the angle head. 2. Insert the capillary holder into the clamp. 3. Align the capillary holder in such a way that the capillary tip is located approx. 20 mm above and approx. 20 mm from the outside of the operating point. 4.
Installation 32 TransferMan® 4r English (EN) Standard capillary: Only use the 0 grip head with capillaries with an outer diameter of 1.0 mm to 1.1 mm. If you would like to use other capillaries, order the matching grip head. 1. Push the capillary into the grip head until it reaches the stop and tighten the grip head. 4.6.1 Inserting Femtotips 1. Remove the grip head. 2. Screw the adapter for Femtotips into the universal capillary holder. 3. Screw the Femtotip into the adapter and tighten. 4.
Installation TransferMan® 4r 33 English (EN) 4.8 Aligning the motor module Centrally align the modules in order to be able to use the full movement range of the modules. 4.8.1 Aligning the height 1. Undo the screw on the YZ-connector. 2. Align the Y-module on the scale of the Z-module and tighten the screw. 4.8.2 Aligning the depth 1. Undo the screw on the swivel joint. 2. Align the X-module on the scale of the Y-module and tighten the screw.
Installation 34 TransferMan® 4r English (EN) 4.8.3 Aligning the width 1. Undo the screw on the Z-module holder. 2. Align the Z-module on the scale of the microscope adapter and tighten the screw. 4.8.4 Aligning the angle head 1. Undo the screw on the angle head. 2. Align the angle head on the scale of the X-module. 4.9 Entering mounting parameters To facilitate remounting the mounting parameters can be recorded. 1. Enter the mounting parameters in the tables. 4.9.
Installation TransferMan® 4r 35 English (EN) 4.9.2 Motor module 1 2 3 4 5 6 7 Position Description 1 Z-module holder on the adapter Position [mm] 2 Z-module on the Z-module holder 3 YZ-connector on the Z-module 4 YZ-connector on the Y-module 5 Swivel joint on the Y-module 6 Swivel joint on the X-module 7 Angle head on the X-module 4.9.
Installation 36 TransferMan® 4r English (EN) 4.10 Converting the swivel joint for left side mounting Prerequisites • 2 mm Allen key • Right side mounting marks (||) are aligned above each other 1. Rotate the lower joint until both Allen screws are accessible. 2. Unscrew the Allen screws.
Installation TransferMan® 4r 37 English (EN) 3. Remove the stop plate. 4. Rotate the lower joint back. 5. Rotate the upper joint by 180°. The sliders must be at a 90° angle to each other.
Installation 38 TransferMan® 4r English (EN) 6. Align the left side mounting marks (I). 7. Fit the stop plate in such a way that the pins sit in the holes of the turntable. 8. Rotate the swivel joint by 180°. 9. Screw down the stop plate. • Left side mounting marks (|) are aligned above each other.
Installation TransferMan® 4r 39 English (EN) 10.Check the position of the joints. • The sliders must be at a 90° angle to each other. 90° 4.11 Converting the angle head for left side mounting Prerequisites • 1.3 mm Allen key 1. Undo the set screw and pull the knurled screw off the spindle.
Installation 40 TransferMan® 4r English (EN) 2. Rotate the angle head by 180°. 3. Push the knurled screw onto the spindle end and fasten it with the set screw. + 4.12 4. Turn the knurled screw until the desired angle is set. Connect the motor module to the control board WARNING! Risk from incorrect supply voltage Only connect the device to power supplies which correspond with the electrical requirements on the name plate.
Installation TransferMan® 4r 41 English (EN) NOTICE! Material damage from incorrect connections. Only electrical connections may be made to devices described in the operating manual. Other connections are permitted only following consultation and agreement with Eppendorf AG. Only connect devices that meet the safety requirements defined in IEC 60950-1. NOTICE! Short circuit caused by incorrect installation. Failure to observe the order of steps may result in a short circuit.
Installation 42 TransferMan® 4r English (EN) 4.13.1 First set-up wizard Prerequisites • The micromanipulator is switched on. 1. Select the First set-up application. 2. Select the mounting side. 3. Select Next. 4. Set the operating angle. 5. Select Next.
Installation TransferMan® 4r 43 English (EN) 6. Select Execute to move all motors to the middle position. 7. Select Next. 8. Insert the capillary holder into the angle head. 9. Select Next. 10.Manually align the modules. 11.Select Next. 12.Remove the capillary holder. 13.Insert the capillary into the capillary holder. 14.Insert the capillary holder with the capillary into the angle head. 15.Finely adjust the position of the capillary holder and the modules. 16.Select Next.
Installation 44 TransferMan® 4r English (EN) 17.Set the time and the date. 18.Select Next. 19.Switch the dynamic movement range of the joystick on or off. 20.Select Next. 21.Select Enter. • The installation is complete and the modules have been adjusted. • The application screen shows My application.
Installation TransferMan® 4r 45 English (EN) 4.14 Connecting an external device The following devices can be connected to the control board: • EppendorfFemtoJet • EppendorfPiezoXpert • Computer 4.14.1 Connecting the FemtoJet Prerequisites • Devices are switched off. The operation is described in the manual for the FemtoJet. 1. Connect the FemtoJet to the port for external devices. 2. Switch on the FemtoJet. The initialization phase starts. 3. Switch on the control board.
Installation 46 TransferMan® 4r English (EN) 4.14.3 Connecting the computer Prerequisites • A data cable is available. • Devices are switched off. Control with a computer is described in the Cell Technology · PC Control manual. 1. Connect the data cable to the port for external devices. 2. Connect the computer to the data cable. 3. Switch on the control board. 4.14.4 Connecting two devices Prerequisites • Y-connector is available. • Devices are switched off.
Software TransferMan® 4r 47 English (EN) 5 5.1 Software Display The display shows current settings, e.g., the selected working range, the position of the motors and the defined limits. 5.1.1 Application display Abb. 5-1:Display layout - ICSI application example 1 6 2 7 3 8 4 5 Fig.
Software 48 TransferMan® 4r English (EN) 5.1.2 Display of coordinates Abb. 5-2:Display of coordinates 1 2 3 4 5 Fig.
Software TransferMan® 4r 49 English (EN) 5.1.3 Menu display Abb. 5-3:Menu and parameter display 1 2 6 3 5 4 Fig. 5-3: Menu and parameter display 1 Navigation path 2 Menu 3 Arrow up/down softkey Navigation and changing parameters.
Software 50 TransferMan® 4r English (EN) 5.2 Applications Abb. 5-4:Application screen Fig. 5-4: Application screen Application selection • Select the application • Store the main application 5.2.1 Application parameters Predefined softkeys of the different applications. • Pos 1 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key. • Pos 2 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key.
Software TransferMan® 4r 51 English (EN) 5.2.2 Application – Cell transfer This application is suitable for, e.g., stem cell transfer, polar body biopsy and similar applications. Abb. 5-5:Application 1 Fig. 5-5: Application 1 Cell transfer Parameter selection • Double-click on joystick key – switch position • Save positions 1, 2 and 3 • Freely program softkey • Set lower limit (Z-axis Limit) 5.2.3 Application – ICSI This application is suitable for intracytoplasmic sperm injection. Abb.
Software 52 TransferMan® 4r English (EN) 5.2.4 Application – DNA injection This application is suitable for injection movements along the horizontal plane (e.g., pronucleus injection). Abb. 5-7:Application 3 Fig. 5-7: Application 3 DNA injection Parameter selection • Double-click on joystick key – switch position • Save positions 1 and 2 • Freely program softkey • Switch off control of movement axis (Y-axis) • Set lower limit (Z-axis Limit) 5.2.
Software TransferMan® 4r 53 English (EN) 5.2.6 Application – My application For this application no softkeys are preprogrammed. The application can be individually programmed. Abb. 5-9:Application 5 Fig. 5-9: Application 5 My application Parameter selection • Freely program joystick key • Freely program all softkeys 5.3 Main menu Abb. 5-10:Main menu Fig.
Software 54 TransferMan® 4r English (EN) 5.4 Navigating the menu Abb. 5-11:Software navigation Fig. 5-11: Software navigation Navigate the menu using the arrow keys. Pressing Enter will confirm the selection. You can switch between menus and submenus with the arrow keys to the left and to the right. 5.4.1 Entering or changing parameters Parameters can be changed in the menu using the arrow keys, the selection dial or the rotating wheel on the joystick. Change values with the upwards or Abb.
Operation TransferMan® 4r 55 English (EN) 6 Operation WARNING! Electric shock due to damage to device or mains cable. Only switch on the device if the device and mains cable are undamaged. Only use devices that have been properly installed or repaired. In case of danger, disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or, by using the isolating device intended for this purpose (e.g., emergency stop switch in the laboratory).
Operation 56 TransferMan® 4r English (EN) • The display shows STANDBY. • Current movements are stopped. • The motor module remains switched on so that the step motors keep their current position. 6.3 Defining the start screen An application can be selected as the default application. The micromanipulator then starts with the defined application. The application screen with all the applications can be redefined in the Start display menu. 6.3.1 Defining the application 1.
Operation TransferMan® 4r 57 English (EN) CAUTION! Risk of injury from capillaries Capillaries can easily penetrate your skin. After changing a capillary, swivel it immediately back to the working range. CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. 2. 3. 4. 5. Move the capillary out of the working range using the home key. Swing the X-module forwards.
Operation 58 TransferMan® 4r English (EN) 6.5 Changing the sample CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. Press the home key to move the capillary out of the working range. 2. Swing the X-module backwards. 3. Change the sample. 4. Swing the X-module back. 5. Press the home key to move the capillary back into the working range. 6.6 Changing the size of the working range 6.6.
Operation TransferMan® 4r 59 English (EN) 6.6.2 Changing parameters in the menu Prerequisites • An application has been selected. 1. 2. 3. 4. Press the menu key. Select the Speed menu. Select the desired parameter. Change the value. 6.7 Shifting the movement range of the capillary 6.7.1 Extending the movement range into the dynamic range When the movement range of the capillary is not sufficient, the joystick can be moved into the dynamic range.
Operation 60 TransferMan® 4r English (EN) 6.8.1 Saving a position Prerequisites • An application has been selected. 1. Move the capillary to the required position. 2. Hold the Pos 1 softkey for approx. one second to store the working position of the capillary. • An acoustic signal will sound. • Pos 1 is marked. • The stored position is displayed in the status field. 3. Move the capillary to the desired position (e.g., parking position). 4. Press and hold the Pos 2 softkey for approx.
Operation TransferMan® 4r 61 English (EN) 6.8.3 Move to the position using the joystick button Prerequisites • At least one position is stored. • The Joystick key parameter must be set to the Switch position value. Press the joystick key twice. It will move to the first position Press the joystick key twice. It will move to the next position 6.8.4 Deleting a stored position Prerequisites • A position is stored. 1. When the position has been reached, hold the softkey. • An acoustic signal will sound.
Operation 62 TransferMan® 4r English (EN) 6.9.1 Defining the lower limit Prerequisites • An application has been selected. 1. Position the capillary a little way above the slide. 2. Press Z-axis Limit. • The Z-coordinate is marked with L. • The value for the limit (Limit Z) is displayed. • Z-axis Limit is selected. • The capillary cannot be moved any lower. 6.9.2 Deleting the lower limit 1. Press Z-axis Limit. • The limit is deleted. 6.9.
Operation TransferMan® 4r 63 English (EN) 6.9.4 Deleting the upper limit 1. In the Installation menu, select the Upper limit submenu. The Upper Limit window appears. 2. Save the position with Clear. • The limit is deleted. 6.10 Horizontal limit For the X-axis, a limit can be defined for a horizontal injection. This prevents the capillary from moving through the sample. 6.10.1 Defining the horizontal limit 1. In the Installation menu, select the Angle submenu. 2.
Operation 64 TransferMan® 4r English (EN) 6.11 Speed function In the Speed menu you can set the size of the working range and the speed at which certain positions are approached or movements executed. 6.11.1 Speed menu and parameters Abb. 6-1:Menu – Fig. 6-1: Menu – Speed Parameter Value Range of values Increment Standard Coarse Set size in μm 5 – 12500 5 Fine Set size in μm 5 – 2000 5 250 X-fine Set size in μm 0 – 600 1 80 Step inj.
Operation TransferMan® 4r 65 English (EN) 6.12 Step injection function With this function you can execute a straight injection over a defined distance. Step injection can only be executed when a FemtoJet is connected. 6.12.1 Step injection menu and parameters Abb. 6-2:Menu – Fig. 6-2: Menu – Step injection Parameter Value Range of values Increment Standard Step injection Switch function on or off OFF/ON Step inj. speed Set inspection speed in μm per 5 – 10000 second – OFF 5 300 Step inj.
Operation 66 TransferMan® 4r English (EN) 6.12.2 Executing the Step injection function Prerequisites • A FemtoJet is connected. • Step injection is assigned to a free softkey. 1. Activate Step injection. 2. Set Step inj. dist. 3. Set the injection time on the FemtoJet. 4. Select Step inj. dist. 5. Close the menu. • The application screen displays Step injection. 6. Trigger the injection by selecting Inject on the FemtoJet. • The function is executed. 6.
Operation TransferMan® 4r 67 English (EN) 6.13.3 Move the capillary out with the home key Prerequisites • The parameters in the Home menu have been defined. CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. Press the home key. The home key flashes. • The capillary is moved out of the work area. • The home key lights up. 6.13.
Operation 68 TransferMan® 4r English (EN) 6.13.6 Terminate the home function. 1. Press Back manual. The home function is terminated. 2. Move the capillary manually using the joystick. 6.14 Clean function With this function you can move the capillary over a defined distance out of the medium. In the process, outside contamination is removed from the capillary at the medium boundary. 6.14.1 Clean menu and parameters Abb. 6-4:Menu – Fig.
Operation TransferMan® 4r 69 English (EN) 6.14.2 Setting the parameter for Clean Set the Clean distance above the medium boundary 1. Set Home speed. 2. Set Clean distance. 3. Close the menu. 6.14.3 Execute the Clean function Prerequisites • Parameters are set for Clean. • Clean is assigned to a softkey. 1. Press Clean. The screen for Clean appears. 2. Execute the function with Clean. • The capillary is moved out of the medium with the set parameters. 6.14.4 Terminate the Clean function. 1.
Operation 70 TransferMan® 4r English (EN) 6.15 PiezoXpert function 6.15.1 PiezoXpert menu and parameters Abb. 6-5:Menu – Fig. 6-5: Menu – PiezoXpert Parameter Value Range of values Increment Standard Synchr. PiezoXp. Switch synchronization between TransferMan 4r and PiezoXpert on or off OFF/ON PiezoXp. speed Set the advance rate of the manipulator in μm/s while the PiezoXpert triggers a pulse. 5 – 10000 5 300 PiezoXp. dist.
Operation TransferMan® 4r 71 English (EN) 6.15.2 Execute the PiezoXpert function Prerequisites • A PiezoXpert is connected. 1. 2. 3. 4. 5. 6. • Activate Synchr. PiezoXp. Set PiezoXp. speed. Set PiezoXp. dist. Select PiezoXp. axial. Close the menu. Trigger the function with Channel A on the PiezoXpert. The function is executed. 6.16 Installation function The function provides settings for making fine adjustments to the motor module and the control board.
Operation 72 TransferMan® 4r English (EN) 6.16.3 Control board parameters Parameter Value Range of values Increment Standard LCD contrast Set the display contrast 65 % – 75 % 1 70 % LCD light Set the brightness level of the display 0 % – 100 % 1 100 % Beeper Set the volume 0 – 100 % 1 50 % Display coordin. Set the coordinate display OFF/ON – On 6.16.
Operation TransferMan® 4r 73 English (EN) 6.17 Function function Function allows you to reset the parameters and coordinates and to move the modules to the middle position. 6.17.1 Function menu and parameters Abb. 6-7:Menu – Fig. 6-7: Menu – Function Parameter Value Range of values Zero coordin. Reset all coordinates to zero Execute Center motors Move step motors of the motor module to the middle position Execute User default Reset the settings to the delivery status Execute 6.17.
Operation 74 TransferMan® 4r English (EN) • • • • Z-motor is moved to a 20/80 position. All coordinates are reset to zero. Saved positions are deleted. Set limits are deleted. 6.17.4 Executing User default 1. 2. • • Select the parameters and confirm with Enter. Confirm Execute with Enter. All parameters are reset to the factory settings. The application screen appears. 6.18 Softkeys function With this function you can assign programs to free softkeys.
Operation TransferMan® 4r 75 English (EN) Parameter Function No function Softkey has no function Pos 1 Save, overwrite or delete a position Pos 2 Save, overwrite or delete a position Pos 3 Save, overwrite or delete a position Pos 4 Save, overwrite or delete a position Pos 5 Save, overwrite or delete a position Y-axis off Switch the control of the movement axis on or off Axial Change vertical movement to axial movement Limit Activate or deactivate the vertical or horizontal (X-axis) limit
Operation 76 TransferMan® 4r English (EN) 6.18.2 Execute Softkeys 1. 2. 3. • • Select the free softkey and confirm with Enter. Select the desired parameter and confirm with Enter. Close the menu. The selected parameter is assigned to the free softkey. The parameter appears on the application screen. 6.18.3 Execute Joystick key Prerequisites The My application application has been selected. 1. 2. 3. • • Select Joystick key and confirm with Enter. Select the desired function and confirm with Enter.
Operation TransferMan® 4r 77 English (EN) 6.19.2 Defining the selected application as the start screen 1. 2. 3. • Select Start display. Confirm with Execute. Close the menu. The micromanipulator starts with the selected application. 6.19.3 Defining the application as the start screen 1. 2. 3. • Select the application. Confirm with Enter. Close the menu. The micromanipulator always starts with the defined application. 6.20 Service function 6.20.1 Service menu and parameters Abb. 6-10:Service Fig.
Operation 78 TransferMan® 4r English (EN) 6.20.2 Execute the Selftest function CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. Select Selftest and confirm with Enter. 2. Confirm Execute with Enter. The screen for the Selftest function appears. 3. Execute Selftest with Start. The motors move up to the limit stops. 4. Terminate Selftest with Stop. 6.
Troubleshooting TransferMan® 4r 79 English (EN) 7 Troubleshooting 7.1 General errors 7.1.1 Motor module Symptom/message Cause • Motor module mounted Motor axes move in incorrectly. the wrong direction or do not match joystick • Incorrect installation movement. parameters entered. • Direction of movement of axis inverted. 7.1.2 Remedy Compare the installation parameters with the structure of the motor module. Check the orientation and structure of the modules.
Troubleshooting 80 TransferMan® 4r English (EN) Symptom/message Cause • The power cable or power The display does not plug is loose. show anything or the device cannot be • The device is switched off. activated although the device is connected. Remedy Check the power cable or the power plug. Switch the device on. 7.1.4 Joystick Symptom/message Cause Outer movement range • Outer movement range is deactivated. of the joystick does not work.
Troubleshooting TransferMan® 4r 81 English (EN) Symptom/message Cause Remedy WARNING 5 • Z-module is not connected. Switch off the device. Connect the plug of the Z-module to the control board and tighten. Switch the device on. WARNING 6 • Synchronization error with FemtoJet during an injection. Set another synchronization for Synchr. inject.. Set the synchronization IMMEDIATE, LIMIT or PRESSURE. 7.2.2 Error Symptom/message Cause ERROR 10 – ERROR 99 • Technical error.
Maintenance 82 TransferMan® 4r English (EN) 8 8.1 Maintenance Replacing fuses DANGER! Electric shock. Switch off the device and disconnect the power plug before starting maintenance or cleaning work. The fuse holder is located between the mains connection socket and the mains power switch. The fuses may only be replaced with the same type of fuse. 1. 2. 3. 4. Disconnect the mains plug. Pull the fuse holder out completely. Replace the defective fuse. Insert the fuse holder. 8.
Maintenance TransferMan® 4r 83 English (EN) 8.3 Service and maintenance Application-specific service and safety inspections are not required The Eppendorf AG service team is available to service and certify your device. Service provisions: • Service • Operational qualification (OQ) according to manufacturer's specifications • Electrical safety testing according to country-specific regulations • Software update Information on the services offered can be found on our webpage: www.eppendorf.com/ epservices.
Technical data 84 TransferMan® 4r English (EN) 9 Technical data Motor module Travel, maximum 20 mm Step motors X-module, Y-module, Z-module Weight 2150 g Module (X,Y,Z) Type Step motors Step size (calculated resolution) < 20 nm Speed, maximum 10000 μm/s Width 129 mm Depth 51 mm Height 36 mm Weight 570 g Swivel joint Direction of rotation -45° – +90° Capillary change Direction of rotation forwards Sample change Direction of rotation backwards Angle head Operating angle 0° – 90°
Technical data TransferMan® 4r 85 English (EN) 9.1 Power supply Control board Mains connection 120 – 240 V AC, 50 – 60 Hz Power consumption 30 W Protection class I Overvoltage category II (IEC 61010-1) Micro fuse T 1.6 A, 250 V 9.2 Interfaces Module (X, Y, Z) SubD25, female PC/external device Serial interface SubD9, male Service connection USB 9.3 Ambient conditions Environment For indoor use only. Ambient temperature 15 °C – 35 °C Max.
Transport, storage and disposal 86 TransferMan® 4r English (EN) 10 10.1 Transport, storage and disposal Storage Air temperature Relative humidity Atmospheric pressure In transport packaging -25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa Without transport packaging -5 °C – 45 °C 10 % – 95 % 70 kPa – 106 kPa 10.
Transport, storage and disposal TransferMan® 4r 87 English (EN) 10.4 Disposal In case the product is to be disposed of, the relevant legal regulations are to be observed. Information on the disposal of electrical and electronic devices in the European Community: Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).
Ordering Information 88 TransferMan® 4r English (EN) 11 11.1 Ordering Information TransferMan 4r Order no. (International) Order no. (North Description America) 5193 000.012 5193 000.020 5193 000.039 5193 000.047 5193 000.055 5193 000.063 5193000012 5193000020 5193000039 5193000047 5193000055 5193000063 11.
Ordering Information TransferMan® 4r 89 English (EN) 11.3 Tools for TransferMan 4r Order no. (International) Order no. (North Description America) 5192 074.004 5192074004 Allen torque screwdriver 3 mm 5192 075.000 5192075000 Allen screwdriver 1.3 mm 5192 076.007 5192076007 Allen key 7 pieces 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6mm 5192 077.003 5192077003 Toolbag 11.4 Microscope adapter Order no. (International) 5192 301.000 5192 302.007 5192 306.002 5192 307.009 5192 316.
Ordering Information 90 TransferMan® 4r English (EN) 11.5 Accessories for adapters for microscope Order no. (International) Order no. (North Description America) 5192 325.007 5192325007 Universal stand for micromanipulators TransferMan 4m/4r, InjectMan 4 5192 321.001 5192321001 Adapter bridge TransferMan 4m/4r, InjectMan 4 11.6 Capillaries for research applications Order no. (International) Order no. (North Description America) 5242 956.003 930001007 epT.I.P.S.
Ordering Information TransferMan® 4r 91 English (EN) Order no. (International) Order no. (North Description America) 5175 240.006 5175240006 VacuTips FCH 25 pieces 5175 114.000 930001074 TransferTip-RP (ICSI) 25 pieces, sterile 5175 106.008 930001031 TransferTip-F (ICSI) 25 pieces, sterile 5175 113.004 930001066 TransferTip-R (ICSI) 25 pieces, sterile 5175 210.000 5175210000 Polar Body Biopsy Tip MML 25 pieces 5175 230.000 5175230000 Polar Body Biopsy Tip FCH 25 pieces 5175 117.
Ordering Information 92 TransferMan® 4r English (EN) Order no. (International) 5176 195.004 11.9 Order no. (North Description America) 920005888 Service kit For grip heads and capillary holder Consisting of O-ring set, adapter for Femtotips CellTram and accessories Products are registered as a medical device in Europe (according to the Medical Device Directive (MDD) 93/42/EEC). Order no. (International) Order no. (North Description America) 5176 000.017 920002021 CellTram Air 5176 000.
Ordering Information TransferMan® 4r 93 English (EN) 11.11 FemtoJet express Order no. (International) 5248 000.017 5248 000.033 5248 000.041 5248 000.050 5248 000.076 5248 000.084 Order no. (North Description America) 5248000017 5248000033 5248000041 920010521 5248000076 5248000084 FemtoJet express programmable micro-injector Mains/Power plug Europe Mains/Power plug UK / Hongkong Mains/Power plug Japan Mains/Power plug USA and Canada Mains/Power plug Australia Mains/Power plug China 11.
Ordering Information 94 TransferMan® 4r English (EN) 11.13 PiezoXpert Order no. (International) 5194 000.016 5194 000.024 5194 000.032 5194 000.059 5194 000.067 5194 000.075 Order no.
Index TransferMan® 4r 95 English (EN) Index A Ambient conditions ................................ 85 Application screen.................................. 50 C Cable....................................................... 40 Capillary Replace ............................................. 56 Cleaning ................................................. 82 Connection External device ................................. 45 D Decontamination .................................... 86 Disinfectant ........................
Index 96 TransferMan® 4r English (EN) Storage ................................................... 86 Switching off .......................................... 55 Switching on........................................... 55 U Unpacking .............................................. 22 Upper Limit ............................................ 62 X X-limit..................................................... 63 Z Z limit .....................................................
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