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Copyright© 2013 Eppendorf AG, Hamburg. Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. TransferMan® 4m, FemtoJet® and Eppendorf PiezoXpert® are registered trademarks of Eppendorf AG, Hamburg, Germany. Registered trademarks are not marked in all cases with ™ or ® in this manual. No part of this publication may be reproduced without the prior permission of the copyright owner. 5191 900.
Table of contents TransferMan® 4m 3 English (EN) Table of contents Operating TransferMan® on see p. Tab. Fig. p. manual 4m English Operating (EN) manual 1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2.
Table of contents 4 TransferMan® 4m English (EN) 4.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.1 Mounting the Z-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.2 Mounting the Y-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.3 Mounting the X-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.4.4 Mounting the angle head . . . . . . . . . . . . . . . . . . . . . . . .
Table of contents TransferMan® 4m 5 English (EN) 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 6.10 6.11 6.12 6.13 Activating or deactivating the control board. . . . . . . . . . . . . . . . . . . . . . . . . 6.2.1 Activating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2.2 Deactivating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . Defining the start screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.3.
Table of contents 6 TransferMan® 4m English (EN) 6.14 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.14.1 Clean menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.14.2 Setting the parameter for Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 6.14.3 Execute the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.14.4 Terminate the Clean function. . . . . . . . . . . . . . . . .
Table of contents TransferMan® 4m 7 English (EN) 9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Operating instructions 8 TransferMan® 4m English (EN) 1 1.1 Operating instructions Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. This operating manual is part of the product. Thus, it must always be easily accessible. Enclose this operating manual when transferring the device to third parties. If this manual is lost, please request another one.
Product description TransferMan® 4m 9 English (EN) 2 2.1 Product description Delivery package Quantity Description 1 X-module 1 Y-module 1 Z-module 1 YZ-connector 1 Swivel joint 1 Angle head 1 Control board 1 Mains/power cord 1 Cable sheathing 1 Operating manual 2.1.1 Tools Quantity Description 7 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm Allen key 1 3 mm Allen torque key 1 1.3 mm Allen key 1 Tool bag 2.1.
Product description 10 TransferMan® 4m English (EN) transferred directly to the capillary. In the outer range, a greater forwards or backwards movement of the joystick results in an acceleration of the capillary movement. The movement ranges enable moving to any position in the working range of the micromanipulator. Proportional movement is suitable for all working techniques that require intuitive, sensitive handling, e.g.
Product description TransferMan® 4m 11 English (EN) 2.3.1 Motor module The motor module is made up of three modules. The capillary can be moved in all three spatial axes due to the layout of the modules. The X-module with the capillary can be swung out of the work area using the swivel joint. The injection angle of the capillary can be set to any angle on the angle head. Abb. 2-2:Motor module 1 4 2 5 6 3 Fig.
Product description 12 TransferMan® 4m English (EN) 2.3.2 Control board The control board contains the key pad, the display and the joystick, and, to the side, the selection dial. The direction of movement and the speed of the joystick are transferred to the capillary. The responsitivity of the movement and the size of the working range are predefined in the software setting. On the control panel, the working range can be selected and individually changed using the selection dial. Abb.
Product description TransferMan® 4m 13 English (EN) Abb. 2-4:Control board - rear 2 1 ext. Device Z-Axis 3 Y-Axis X-Axis SERVICE 8 Fig.
Product description 14 TransferMan® 4m English (EN) 2.4 Control panel With the keys on the control panel you can switch on the control board and select the size of the working range. Via the softkeys you can open the applications, execute the functions, navigate the menu and set the parameters. Abb. 2-5:Control panel 7 6 5 coarse home 1 2 fine x–fine 3 menu 4 Fig.
Product description TransferMan® 4m 15 English (EN) 2.5 Joystick The joystick moves the capillary in all three spatial axes. The joystick movement is transferred directly to the capillary in the proportional range. In the dynamic range, the movement of the capillary is accelerated the further the joystick is moved forwards and backwards. Abb. 2-6:Joystick 1 2 3 Fig. 2-6: Joystick 1 Joystick key 2 2 Swivel Controls movements in the Z-axis Lower part Controls movements in the X and Y-axis Abb.
Product description 16 TransferMan® 4m English (EN) 2.5.1 Proportional range In the proportional range, the capillary will move as quickly or slowly as the joystick is moved. The travel of the capillary is also proportional to the distance that the joystick has been moved forwards and backwards. The movement of the capillary will stop as soon as the joystick is no longer moved or when the position is reached at which the joystick was stopped.
Product description TransferMan® 4m 17 English (EN) 2.5.4 Direction of movement of the rotating wheel The rotating wheel on the joystick moves the motor module in the vertical axis. This activates the motor of the Z-module. Move the capillary in the vertical Abb. 2-9:Movement in the Z-axis direction (Z-axis). Fig. 2-9: 2.5.5 Movement in the Z-axis Joystick key functions Uncouple the joystick from the motor Abb. 2-10:No movement of the capillary module. Trigger functions (e.g.
Product description 18 TransferMan® 4m English (EN) Move capillary over a large distance. Position capillary roughly and quickly. Abb. 2-11:Large working range – coarse Fig. 2-11: Large working range – coarse Move capillary over a medium distance. Position capillary precisely. Abb. 2-12:Medium working range – fine Fig. 2-12: Medium working range – fine Move capillary over a very short Abb. 2-13:Small working range – x-fine distance. Position capillary very precisely and slowly.
Product description TransferMan® 4m 19 English (EN) 2.7 Resulting speed In the inner (proportional) range, the speed of the capillary is dependent on the speed at which the joystick is moved forwards and backwards and the size of the set working range. If the joystick is moved forwards and backwards at the same speed with, e.g., the setting fine or coarse, the resulting speed with the smaller fine working range is less than with the larger coarse range.
Safety 20 TransferMan® 4m English (EN) 3 3.1 Safety Intended use The TransferMan 4m has been designed and manufactured for use in the field of intracytoplasmic sperm injection (ICSI) in human reproductive medicine. Therefore, the TransferMan 4m is a medical device in accordance with Directive 93/42/EEC of the European Union. The TransferMan 4m is intended exclusively for indoor use and for operation by qualified staff. 3.
Safety TransferMan® 4m 21 English (EN) WARNING! Damages to health due to infectious liquids and pathogenic germs. When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes. Wear personal protective equipment.
Installation 22 TransferMan® 4m English (EN) 4 4.1 Installation Preparing installation NOTICE! Damage to the control board as a result of incorrect handling. Grasp the control board on the housing. Do not lift the control board using the joystick. Never place the control board on the joystick. Keep the packaging and the transport securing devices for later transport or storage. Do not operate the device if there is visible damage to the device itself and/or to its packaging. 1. 2. 3. 4.
Installation TransferMan® 4m 23 English (EN) 4.3 Mounting overview Abb. 4-1:Overview for right-hand side mounting 1 2 4 3 10 Fig.
Installation 24 TransferMan® 4m English (EN) 4.3.1 Module (X, Y, Z) Abb. 4-2:Y-module 2 1 3 4 Y 5 Fig.
Installation TransferMan® 4m 25 English (EN) 4.3.2 Z-module holder Abb. 4-3:Z-module holder, front and back 2 1 3 4 Fig. 4-3: Z-module holder, front and back 1 Slider 3 2 Screw Attach Z-module Screw Attach Z-module holder to the adapter 4 Stop angle 4.3.3 Slider Abb. 4-4:Slider 1 Fig.
Installation 26 TransferMan® 4m English (EN) 4.3.4 Angle head Abb. 4-5:Angle head 1 5 Fig.
Installation TransferMan® 4m 27 English (EN) 4.3.5 Swivel joint Abb. 4-6:Swivel joint for right side mounting 5 6 7 1 2 3 8 4 Fig. 4-6: Swivel joint for right side mounting 1 Slider Y-module 2 Turntable 3 Allen screws 4 Slider X-module 4.4 5 Stop plate 6 Upper joint 7 Mounting mark | stands for left side mounting || stands for right side mounting 8 Lower joint Mounting the motor module The motor module can be mounted on the right-hand or left-hand side of the microscope adapter.
Installation 28 TransferMan® 4m English (EN) 4.4.1 Mounting the Z-module Prerequisites • 3 mm Allen torque key 1. Push the Z-module holder onto the microscope adapter and tighten. 2. Push the fixed rail onto the Z-module holder until it reaches the stop angle and tighten the screw. The connecting cable faces to the rear. 4.4.
Installation TransferMan® 4m 29 English (EN) 1. Push the YZ-connector onto the fixed rail of the Y-module and tighten. 2. Push the Y-module with the YZ-connector onto the Z-module and tighten. 4.4.3 Mounting the X-module Prerequisites • 3 mm Allen torque key 1. Push the swivel joint onto the fixed rail of the X-module and tighten.
Installation 30 TransferMan® 4m English (EN) 2. Push the swivel joint with the X-module onto the Y-module and tighten. 4.4.4 Mounting the angle head Prerequisites • 3 mm Allen torque key 1. Push the angle head onto the X-module. 2. Slightly tighten the angle head. 4.5 Inserting the capillary holder into the angle head Prerequisites • A universal capillary holder from Eppendorf is available. • A capillary holder (4 mm diameter) from a different manufacturer is available.
Installation TransferMan® 4m 31 English (EN) 1. Loosen the knurled screw on the angle head. 2. Insert the capillary holder into the clamp. 3. Align the capillary holder in such a way that the capillary tip is located approx. 20 mm above and approx. 20 mm from the outside of the operating point. 4. Place the positioning aid on the capillary holder and tighten. 5. Tighten the knurled screw. 4.
Installation 32 TransferMan® 4m English (EN) 1. Push the capillary into the grip head until it reaches the stop and tighten the grip head. 4.6.1 Inserting Femtotips 1. Remove the grip head. 2. Screw the adapter for Femtotips into the universal capillary holder. 3. Screw the Femtotip into the adapter and tighten. 4.7 Setting the injection angle + 1. Turn the knurled screw to set the injection angle.
Installation TransferMan® 4m 33 English (EN) 4.8 Aligning the motor module Centrally align the modules in order to be able to use the full movement range of the modules. 4.8.1 Aligning the height 1. Undo the screw on the YZ-connector. 2. Align the Y-module on the scale of the Z-module and tighten the screw. 4.8.2 Aligning the depth 1. Undo the screw on the swivel joint. 2. Align the X-module on the scale of the Y-module and tighten the screw.
Installation 34 TransferMan® 4m English (EN) 4.8.3 Aligning the width 1. Undo the screw on the Z-module holder. 2. Align the Z-module on the scale of the microscope adapter and tighten the screw. 4.8.4 Aligning the angle head 1. Undo the screw on the angle head. 2. Align the angle head on the scale of the X-module. 4.9 Entering mounting parameters To facilitate remounting the mounting parameters can be recorded. 1. Enter the mounting parameters in the tables. 4.9.
Installation TransferMan® 4m 35 English (EN) 4.9.2 Motor module 1 2 3 4 5 6 7 Position Description Position [mm] 1 Z-module holder on the adapter 2 Z-module on the Z-module holder 3 YZ-connector on the Z-module 4 YZ-connector on the Y-module 5 Swivel joint on the Y-module 6 Swivel joint on the X-module 7 Angle head on the X-module 4.9.
Installation 36 TransferMan® 4m English (EN) 4.10 Converting the swivel joint for left side mounting Prerequisites • 2 mm Allen key • Right side mounting marks (||) are aligned above each other 1. Rotate the lower joint until both Allen screws are accessible. 2. Unscrew the Allen screws.
Installation TransferMan® 4m 37 English (EN) 3. Remove the stop plate. 4. Rotate the lower joint back. 5. Rotate the upper joint by 180°. The sliders must be at a 90° angle to each other.
Installation 38 TransferMan® 4m English (EN) 6. Align the left side mounting marks (I). 7. Fit the stop plate in such a way that the pins sit in the holes of the turntable. 8. Rotate the swivel joint by 180°. 9. Screw down the stop plate. • Left side mounting marks (|) are aligned above each other.
Installation TransferMan® 4m 39 English (EN) 10.Check the position of the joints. • The sliders must be at a 90° angle to each other. 90° 4.11 Converting the angle head for left side mounting Prerequisites • 1.3 mm Allen key 1. Undo the set screw and pull the knurled screw off the spindle.
Installation 40 TransferMan® 4m English (EN) 2. Rotate the angle head by 180°. 3. Push the knurled screw onto the spindle end and fasten it with the set screw. + 4.12 4. Turn the knurled screw until the desired angle is set. Connect the motor module to the control board WARNING! Risk from incorrect supply voltage Only connect the device to power supplies which correspond with the electrical requirements on the name plate.
Installation TransferMan® 4m 41 English (EN) NOTICE! Material damage from incorrect connections. Only electrical connections may be made to devices described in the operating manual. Other connections are permitted only following consultation and agreement with Eppendorf AG. Only connect devices that meet the safety requirements defined in IEC 60950-1. NOTICE! Short circuit caused by incorrect installation. Failure to observe the order of steps may result in a short circuit.
Installation 42 TransferMan® 4m English (EN) 4.13.1 First set-up wizard Prerequisites • The micromanipulator is switched on. 1. Select the First set-up application. 2. Select the mounting side. 3. Select Next. 4. Set the operating angle. 5. Select Next.
Installation TransferMan® 4m 43 English (EN) 6. Select Execute to move all motors to the middle position. 7. Select Next. 8. Insert the capillary holder into the angle head. 9. Select Next. 10.Manually align the modules. 11.Select Next. 12.Remove the capillary holder. 13.Insert the capillary into the capillary holder. 14.Insert the capillary holder with the capillary into the angle head. 15.Finely adjust the position of the capillary holder and the modules. 16.Select Next.
Installation 44 TransferMan® 4m English (EN) 17.Set the time and the date. 18.Select Next. 19.Switch the dynamic movement range of the joystick on or off. 20.Select Next. 21.Select Enter. • The installation is complete and the modules have been adjusted. • The application screen shows My application.
Software TransferMan® 4m 45 English (EN) 5 5.1 Software Display The display shows current settings, e.g., the selected working range, the position of the motors and the defined limits. 5.1.1 Application display Abb. 5-1:Display layout - ICSI application example 1 6 2 7 3 8 4 5 Fig.
Software 46 TransferMan® 4m English (EN) 5.1.2 Display of coordinates Abb. 5-2:Display of coordinates 1 2 3 4 5 Fig.
Software TransferMan® 4m 47 English (EN) 5.1.3 Menu display Abb. 5-3:Menu and parameter display 1 2 6 3 5 4 Fig. 5-3: Menu and parameter display 1 Navigation path 2 Menu 3 Arrow up/down softkey Navigation and changing parameters. 5.2 Applications Abb. 5-4:Application screen Fig.
Software 48 TransferMan® 4m English (EN) Application selection • Select the application • Store the main application 5.2.1 Application parameters Predefined softkeys of the different applications. • Pos 1 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key. • Pos 2 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key. • Pos 3 – Save the X, Y and Z values of the capillary position.
Software TransferMan® 4m 49 English (EN) 5.2.3 Application – ICSI This application is suitable for intracytoplasmic sperm injection. Abb. 5-6:Application 2 ICSI Fig. 5-6: Application 2 ICSI Parameter selection • Double-click on joystick key – switch position • Save positions 1 and 2 • Freely program softkey • Switch off control of movement axis (Y-axis) • Set lower limit (Z-axis Limit) 5.2.
Software 50 TransferMan® 4m English (EN) 5.2.5 Application – Basic This application is suitable for injection movements into larger organisms (e.g., Drosophila embryos). Abb. 5-8:Application 4 Basic Fig. 5-8: Application 4 Basic Parameter selection • Double-click on joystick key – switch position • Save position 1 • Freely program softkey • Switch on axial movement of Z-axis • Freely program softkey • Set lower limit (Z-axis Limit) 5.2.
Software TransferMan® 4m 51 English (EN) 5.3 Main menu Abb. 5-10:Main menu Fig. 5-10: Main menu Menu Parameter Speed Set the working range and the speed parameters Step injection Set the injection parameters Home Set the parameters for the Home movement Clean Set the parameters for the Clean movement PiezoXpert Set the parameters for an optional device Installation Set the device parameters Function Execute the device function Softkeys Program the softkeys Change appl.
Software 52 TransferMan® 4m English (EN) 5.4 Navigating the menu Abb. 5-11:Software navigation Fig. 5-11: Software navigation Navigate the menu using the arrow keys. Pressing Enter will confirm the selection. You can switch between menus and submenus with the arrow keys to the left and to the right. 5.4.1 Entering or changing parameters Parameters can be changed in the menu using the arrow keys, the selection dial or the rotating wheel on the joystick. Change values with the upwards or Abb.
Operation TransferMan® 4m 53 English (EN) 6 Operation WARNING! Electric shock due to damage to device or mains cable. Only switch on the device if the device and mains cable are undamaged. Only use devices that have been properly installed or repaired. In case of danger, disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or, by using the isolating device intended for this purpose (e.g., emergency stop switch in the laboratory).
Operation 54 TransferMan® 4m English (EN) • Current movements are stopped. • The motor module remains switched on so that the step motors keep their current position. 6.3 Defining the start screen An application can be selected as the default application. The micromanipulator then starts with the defined application. The application screen with all the applications can be redefined in the Start display menu. 6.3.1 Defining the application 1.
Operation TransferMan® 4m 55 English (EN) CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. 2. 3. 4. 5. Move the capillary out of the working range using the home key. Swing the X-module forwards. Undo the grip head on the capillary holder. Carefully pull the capillary out of the grip head. Push the new capillary into the grip head until it reaches the stop and tighten the grip head. 6.
Operation 56 TransferMan® 4m English (EN) 6.5 Changing the sample CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. Press the home key to move the capillary out of the working range. 2. Swing the X-module backwards. 3. Change the sample. 4. Swing the X-module back. 5. Press the home key to move the capillary back into the working range. 6.6 6.6.
Operation TransferMan® 4m 57 English (EN) 6.6.2 Changing parameters in the menu Prerequisites • An application has been selected. 1. 2. 3. 4. 6.7 6.7.1 Press the menu key. Select theSpeed menu. Select the desired parameter. Change the value. Shifting the movement range of the capillary Extending the movement range into the dynamic range When the movement range of the capillary is not sufficient, the joystick can be moved into the dynamic range.
Operation 58 TransferMan® 4m English (EN) 6.8.1 Saving a position Prerequisites • An application has been selected. 1. Move the capillary to the required position. 2. Hold the Pos 1 softkey for approx. one second to store the working position of the capillary. • An acoustic signal will sound. • Pos 1 is marked. • The stored position is displayed in the status field. 3. Move the capillary to the desired position (e.g., parking position). 4. Press and hold the Pos 2 softkey for approx.
Operation TransferMan® 4m 59 English (EN) 6.8.3 Move to the position using the joystick button Prerequisites • At least one position is stored. • The Joystick key parameter must be set to the Switch position value. Press the joystick key twice. It will move to the first position Press the joystick key twice. It will move to the next position 6.8.4 Deleting a stored position Prerequisites • A position is stored. 1. When the position has been reached, hold the softkey.
Operation 60 TransferMan® 4m English (EN) 6.9.1 Defining the lower limit Prerequisites • An application has been selected. 1. Position the capillary a little way above the slide. 2. Press Z-axis Limit. • The Z-coordinate is marked with L. • The value for the limit (Limit Z) is displayed. • Z-axis Limit is selected. • The capillary cannot be moved any lower. 6.9.2 Deleting the lower limit 1. Press Z-axis Limit. • The limit is deleted. 6.9.
Operation TransferMan® 4m 61 English (EN) • The value for the limit (Lim) is displayed. • The capillary cannot be moved any higher. 6.9.4 Deleting the upper limit 1. In the Installation menu, select the Upper limit submenu. The Upper Limit window appears. 2. Save the position with Clear. • The limit is deleted. 6.10 Horizontal limit For the X-axis, a limit can be defined for a horizontal injection. This prevents the capillary from moving through the sample. 6.10.1 Defining the horizontal limit 1.
Operation 62 TransferMan® 4m English (EN) • The lateral limit is deleted. • The application screen displays Back again. 6.11 Speed function In the Speed menu you can set the size of the working range and the speed at which certain positions are approached or movements executed. 6.11.1 Speed menu and parameters Abb. 6-1:Menu – Speed Fig.
Operation TransferMan® 4m 63 English (EN) 6.12 Step injection function With this function you can execute a straight injection over a defined distance. Step injection can only be executed when a FemtoJet is connected. 6.12.1 Step injection menu and parameters Abb. 6-2:Menu – Step injection Fig. 6-2: Menu – Step injection Parameter Value Range of values Increment Standard Step injection Switch function on or off OFF/ON – OFF Step inj.
Operation 64 TransferMan® 4m English (EN) 6.12.2 Executing the Step injection function Prerequisites • A FemtoJet is connected. • Step injection is assigned to a free softkey. 1. 2. 3. 4. 5. Activate Step injection. Set Step inj. dist. Set the injection time on the FemtoJet. Select Step inj. dist. Close the menu. • The application screen displays Step injection. 6. Trigger the injection by selecting Inject on the FemtoJet. • The function is executed. 6.
Operation TransferMan® 4m 65 English (EN) 6.13.2 Setting the parameter for Home 1. Set the speed. 2. Set the offset. 3. Close the menu. 6.13.3 Move the capillary out with the home key Prerequisites • The parameters in the Home menu have been defined. CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. Press the home key. The home key flashes. • The capillary is moved out of the work area.
Operation 66 TransferMan® 4m English (EN) 6.13.5 Setting the offset. For capillaries of different lengths, an offset can be set. This defines a limit for the home function. 1. Change the offset using the arrow keys. • The value for the offset is displayed in the status field. 6.13.6 Terminate the home function. 1. Press Back manual. The home function is terminated. 2. Move the capillary manually using the joystick. 6.
Operation TransferMan® 4m 67 English (EN) 6.14.1 Clean menu and parameters Abb. 6-4:Menu – Clean Fig. 6-4: Menu – Clean Parameter Value Range of values Increment Standard Home speed Set the speed of the clean function 5 – 10000 5 7500 0 – 20000 5 10000 Clean distance Set distance in μm 6.14.2 Setting the parameter for Clean Set the Clean distance above the medium boundary 1. Set Home speed. 2. Set Clean distance. 3. Close the menu. 6.14.
Operation 68 TransferMan® 4m English (EN) 6.14.4 Terminate the Clean function. 1. Press Back manual. • The function is terminated. • The application screen appears. 6.15 6.15.1 PiezoXpert function PiezoXpert menu and parameters Abb. 6-5:Menu – PiezoXpert Fig. 6-5: Menu – PiezoXpert Parameter Value Range of values Synchr. PiezoXp. Switch synchronization between TransferMan 4m and PiezoXpert on or off OFF/ON PiezoXp.
Operation TransferMan® 4m 69 English (EN) Parameter Value Range of values ON/OFF PiezoXp. axial Axial movement on or off (corresponds to the set injection angle) On = movement in the X and Y-axis. Off = movement in the X-axis 6.15.2 Increment Standard – OFF Execute the PiezoXpert function Prerequisites • A PiezoXpert is connected. 1. 2. 3. 4. 5. 6. • Activate Synchr. PiezoXp. Set PiezoXp. speed. Set PiezoXp. dist. Select PiezoXp. axial. Close the menu.
Operation 70 TransferMan® 4m English (EN) 6.16.2 Installation parameters Parameter Value Range of values Increment Standard Side Set the mounting side LEFT/RIGHT – RIGHT Angle Set the capillary angle in degrees 0° – 90° 1 – 6.16.
Operation TransferMan® 4m 71 English (EN) Parameter Value Range of values Increment Standard Z-axis off Switch the motor of the movement axis on or off OFF/ON – OFF Upper limit Define the upper limit Execute – – 6.17 Function function Function allows you to reset the parameters and coordinates and to move the modules to the middle position. 6.17.1 Function menu and parameters Abb. 6-7:Menu – Function Fig. 6-7: Menu – Function Parameter Value Range of values Zero coordin.
Operation 72 TransferMan® 4m English (EN) 6.17.3 Executing Center motors Prerequisites • No capillary holder is clamped. CAUTION! Risk of crushing between the modules The modules automatically move in all spatial axes. Do not reach into the movement range of the modules. 1. 2. • • • • • Select the parameters and confirm with Enter. Confirm Execute with Enter. X-motor and Y-motor are moved to the middle position. Z-motor is moved to a 20/80 position. All coordinates are reset to zero.
Operation TransferMan® 4m 73 English (EN) Parameter Value Softkey 1 Set the function Softkey 2 Set the function Softkey 3 Set the function Softkey 4 Set the function Softkey 5 Set the function Joystick key Set the function for the joystick key Parameter Function No function Softkey has no function Pos 1 Save, overwrite or delete a position Pos 2 Save, overwrite or delete a position Pos 3 Save, overwrite or delete a position Pos 4 Save, overwrite or delete a position Pos 5 Save, o
Operation 74 TransferMan® 4m English (EN) Parameter Function Dyn-mode Activate or deactivate dynamic movement range Parameter Range of values Function Joystick key No function Deactivate the function of the joystick key Switch position Double-click to go to the next saved position Switch fine Double-click to go to the fine or x-fine working range 6.18.2 1. 2. 3. • • Execute Softkeys Select the free softkey and confirm with Enter. Select the desired parameter and confirm with Enter.
Operation TransferMan® 4m 75 English (EN) 6.19 Change appl. function With this function you can define each application as the start screen or reactivate the default screen. 6.19.1 Change appl. menu and parameters Abb. 6-9:Menu – Change appl. Fig. 6-9: Menu – Change appl. Parameter Value Start display Reactivate start screen to select an application Execute Application Define the selected application as the start screen Cell transfer ICSI DNA injection Basic My application 6.19.2 1. 2. 3.
Operation 76 TransferMan® 4m English (EN) 6.20 6.20.1 Service function Service menu and parameters Abb. 6-10:Service Fig.
Operation TransferMan® 4m 77 English (EN) 6.21 6.21.1 Resetting parameters to the factory settings Perform reset Reset all parameters to the factory settings. 1. Keep the home key pressed. 2. Switch on the device at the mains power switch. The screen for GENERAL RESET appears. 3. Execute the function with Yes. • All parameters are now reset to the factory settings. 6.21.2 Carrying out a reset in the menu Prerequisites • An application has been selected. 1. 2. 3. 4. • Press the menu key.
Troubleshooting 78 TransferMan® 4m English (EN) 7 7.1 Troubleshooting General errors Symptom/message Cause Motor axes move in the • Motor module mounted incorrectly. wrong direction or do not match joystick • Incorrect installation movement. parameters entered. • Direction of movement of axis inverted. Parameters are not accessible for certain operations. – Enter parameters again. Carry out a reset and reset Press the home key again. The capillary moves down.
Troubleshooting TransferMan® 4m 79 English (EN) 7.2 7.2.1 Error messages Warnings Symptom/message Cause Remedy WARNING 1 • FemtoJet is not connected. Disconnect the data cable to the FemtoJet and connect it again. Switch on the FemtoJet. WARNING 3 • X-module is not connected. Switch off the device. Connect the plug of the X-module to the control board and tighten. Switch the device on. WARNING 4 • Y-module is not connected. Switch off the device.
Maintenance 80 TransferMan® 4m English (EN) 8 8.1 Maintenance Replacing fuses DANGER! Electric shock. Switch off the device and disconnect the power plug before starting maintenance or cleaning work. The fuse holder is located between the mains connection socket and the mains power switch. The fuses may only be replaced with the same type of fuse. 1. 2. 3. 4. Disconnect the mains plug. Pull the fuse holder out completely. Replace the defective fuse. Insert the fuse holder. 8.
Maintenance TransferMan® 4m 81 English (EN) 8.3 Service and maintenance Application-specific service and safety inspections are not required The Eppendorf AG service team is available to service and certify your device. Service provisions: • Service • Operational qualification (OQ) according to manufacturer's specifications • Electrical safety testing according to country-specific regulations • Software update Information on the services offered can be found on our webpage: www.eppendorf.com/ epservices.
Technical data 82 TransferMan® 4m English (EN) 9 Technical data Motor module Travel, maximum 20 mm Step motors X-module, Y-module, Z-module Weight 2150 g Module (X,Y,Z) Type Step motors Step size (calculated resolution) < 20 nm Speed, maximum 10000 μm/s Width 129 mm Depth 51 mm Height 36 mm Weight 570 g Swivel joint Direction of rotation -45° – +90° Capillary change Direction of rotation forwards Sample change Direction of rotation backwards Angle head Operating angle 0° – 90°
Technical data TransferMan® 4m 83 English (EN) 9.1 Power supply Control board Mains connection 120 – 240 V AC, 50 – 60 Hz Power consumption 30 W Protection class I Overvoltage category II (IEC 61010-1) Micro fuse T 1.6 A, 250 V 9.2 Interfaces Module (X, Y, Z) SubD25, female PC/external device Serial interface SubD9, male Service connection USB 9.3 Ambient conditions Environment For indoor use only. Ambient temperature 15 °C – 35 °C Max.
Transport, storage and disposal 84 TransferMan® 4m English (EN) 10 10.1 Transport, storage and disposal Storage Air temperature Relative humidity Atmospheric pressure In transport packaging -25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa Without transport packaging -5 °C – 45 °C 10 % – 95 % 70 kPa – 106 kPa 10.
Transport, storage and disposal TransferMan® 4m 85 English (EN) 10.4 Disposal In case the product is to be disposed of, the relevant legal regulations are to be observed. Information on the disposal of electrical and electronic devices in the European Community: Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).
Ordering Information 86 TransferMan® 4m English (EN) 11 11.1 Ordering Information TransferMan 4m Order no. (International) 5191 000.015 5191 000.031 11.2 TransferMan 4m Mains/Power plug Europe Mains/Power plug UK/Hong Kong Accessories for TransferMan 4m Order no. (International) 5181 070.015 5181 150.051 5181 150.094 5181 301.009 5181 303.001 5181 305.004 5181 307.007 5181 309.000 5192 071.005 5192 072.001 11.
Ordering Information TransferMan® 4m 87 English (EN) Order no. (International) 5192 076.007 5192 077.003 11.4 Description Allen key 7 pieces 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6mm Toolbag Microscope adapter Order no. (International) 5192 301.000 5192 302.007 5192 306.002 5192 307.009 5192 316.008 5192 311.006 5192 312.
Ordering Information 88 TransferMan® 4m English (EN) 11.5 Accessories for adapters for microscope Order no. (International) 5192 325.007 5192 321.001 11.6 Description Universal stand for micromanipulators TransferMan 4m/4r, InjectMan 4 Adapter bridge for micromanipulators TransferMan 4m/4r, InjectMan 4 Capillaries Products are registered as a medical device in Europe (according to the Medical Device Directive (MDD) 93/42/EEC). Order no. (International) 5175 108.000 5175 240.006 5175 114.
Ordering Information TransferMan® 4m 89 English (EN) 11.7 CellTram and accessories Products are registered as a medical device in Europe (according to the Medical Device Directive (MDD) 93/42/EEC). Order no. (International) 5176 000.017 5176 000.025 5176 000.033 5176 114.
Index 90 TransferMan® 4m English (EN) Index A Ambient conditions ................................ 83 Application screen.................................. 47 C Cable....................................................... 40 Capillary Replace ............................................. 54 Cleaning ................................................. 80 D Decontamination .................................... 84 Disinfectant ............................................ 81 Display Coordinates ...................
Index TransferMan® 4m 91 English (EN) U Unpacking .............................................. 22 Upper Limit ............................................ 60 X X-limit..................................................... 61 Z Z limit .....................................................
Index 92 TransferMan® 4m English (EN)
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