Operating Temperature Draft on see p. Tab. Fig. p.
Operating manual Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. eppendorf® Mastercycler® and vapoprotect® are registered trademarks of Eppendorf AG, Hamburg, Germany. Trademarks are not marked in all cases with ™ or ® in this manual.
Temperature Verification System USB – Single-channel — Operating manual Table of contents 1 1 Table of contents Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 1.2 1.3 1.4 1.5 2 2.2 2.3 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1.1 Temperature Verification System USB – Single-channel . . . . . . . . . . . . . . . . . 2.1.
Temperature Verification System USB – Single-channel — Operating manual 4.8 5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 5.1 5.2 5.3 6 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 6.1.1 Troubleshooting for the Measuring device . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 6.1.2 Troubleshooting for the Mastercyclers . .
Temperature Verification System USB – Single-channel — Operating manual 1 Operating instructions 1 Operating instructions 1.1 Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. This operating manual is part of the product. Please keep it in a place that is easily accessible. Enclose this operating manual when transferring the device to third parties.
Temperature Verification System USB – Single-channel — Operating manual 1.3 Symbols used Symbol Meaning Handling 1. 2. Actions in the specified order • List Text Name of fields in the software Useful information 1.4 Abbreviations used PDF USB 1.5 Portable document format Universal serial bus Glossary A Adjustment Tuning or alignment of a device to prevent systematic deviations to the extent required for the observance of specifications.
Temperature Verification System USB – Single-channel — Operating manual 2 2 2.1 Product description Product description Delivery package 2.1.1 Temperature Verification System USB – Single-channel For Mastercycler nexus, Mastercycler pro, Mastercycler ep, Thermomixer and ThermoStat 1× measuring device 1× plug head line (connecting cable between measuring device and temperature sensor) 1× mains/power supply device 4× international plug adapter 1× operating manual 1× device case 2.1.
Temperature Verification System USB – Single-channel — Operating manual Device Software version Mastercycler nexus 1.4.0.0 Mastercycler pro 1.023 Mastercycler ep 4.605 CycleManager pro/proXL 2.5/2.6 The Mastercyclers listed feature a precise system which meets all of the laboratory requirements for documentation and the obligation to provide supporting documentation.
Temperature Verification System USB – Single-channel — Operating manual 2.2.4 Temperature sensor 384 Well The Temperature sensor 384 Well and Temperature Verification System USB – Single-channel are used to verify the following devices: • Mastercycler pro 384 • Mastercycler ep 384 • ThermoMixer C • ThermoMixer F1.5 • ThermoMixer FP • ThermoStat C The Mastercycler pro 384 is also adjusted using this sensor. 2.2.
Temperature Verification System USB – Single-channel — Operating manual 3 3 3.1 Safety Safety Intended use The Temperature Verification System USB – Single-channel is a single-channel temperature measuring device for verification and adjustment of thermoblock temperature control and recording the temperature data of thermal cyclers.
Temperature Verification System USB – Single-channel — Operating manual WARNING! CAUTION! CAUTION! CAUTION! WARNING! WARNING! Infection by contaminated material. There may be contaminated material on the device and accessories. Work may only be completed on decontaminated devices. Find out more about contamination risks before beginning work. Check the device decontamination certificate. Wear personal protective equipment (protective gloves, protective goggles).
Temperature Verification System USB – Single-channel — Operating manual NOTICE! 3.5 Warnings for unintended use CAUTION! NOTICE! NOTICE! NOTICE! 12 NOTICE! Material damage from incorrect connections. Only electrical connections may be made to devices described in the operating manual. Other connections are permitted only following consultation and agreement with Eppendorf AG. Only connect devices that meet the safety requirements defined in IEC 60950-1.
Temperature Verification System USB – Single-channel — Operating manual 4 4 4.1 Installation Installation Initial charging of the battery Hint! The Initial setup chapter of the Operating manual of the measuring device contains a description of the initial charging. The charging time can be found in the technical data. The Measuring device is delivered with a partially charged rechargeable battery. Fully charge the battery before using it for the first time. 4.
Temperature Verification System USB – Single-channel — Operating manual 4.3 1. 2. 3. 4. 5. Connecting the Mastercycler nexus Use the temperature sensor connecting cable to connect the grip to the Measuring device. Use the USB cable to connect the Measuring device and the Mastercycler. Switch on the Measuring device. Check the rechargeable battery capacity. Charge the rechargeable battery or use the mains/power supply device if the battery capacity is low.
Temperature Verification System USB – Single-channel — Operating manual 4.4 1. 2. 3. 4. 5. Connecting the Mastercycler pro Use the temperature sensor connecting cable to connect the grip to the Measuring device. Connect the Measuring device and control panel using the USB cable. Switch on the Measuring device. Check the rechargeable battery capacity. Charge the rechargeable battery or use the mains/power supply device if the battery capacity is low.
Temperature Verification System USB – Single-channel — Operating manual 4.5 Connecting the Mastercycler ep Hint! 1. 2. 3. 4. The Mastercycler ep control panel (5340) can only be used for manual verification and adjustment. A direct cable connection cannot be established between the measuring device and thermal cycler. Use the temperature sensor connecting cable to connect the grip to the Measuring device. Switch on the Measuring device. Check the rechargeable battery capacity.
Temperature Verification System USB – Single-channel — Operating manual 4.6.3 Preparing the Thermoblock 384 Prerequisites The thermoblock with 384 wells is cooled to room temperature. Fill the measuring positions with oil. Hint! Oil in the space between the temperature sensor and the thermoblock improves the heat transmission. For measurements with the Temperature Verification System USB – Single-channel, the bores are filled with 5 μL oil at the specified measuring positions.
Temperature Verification System USB – Single-channel — Operating manual Positioning the silicone mat Place the silicon mat over the spacers and the sensor so the thermoblock is evenly covered. Close the heated lid. 4.6.4 Removing oil Remove the oil after the last measurement. The procedure is described in the cleaning chapter (see Removing oil from the thermoblock on p. 33). Connecting the Mastercycler to a computer and CycleManager pro/proXL 4.7 1. 2. 3. 4. 5.
Temperature Verification System USB – Single-channel — Operating manual 5 5 Operation Operation CAUTION! WARNING! NOTICE! CAUTION! Risk of burns on thermoblock, heated lid and temperature sensor. The thermoblock, heated lid and temperature sensor can reach temperatures of more than 95 °C. During gradient operation, the temperature of the heated lid and thermoblock is higher on the right side than on the left. Use extreme caution when conducting measurements on the heated thermoblock.
Temperature Verification System USB – Single-channel — Operating manual 5.1 Verifying the Mastercycler nexus, pro and ep Hint! 5.1.1 Only users who are logged in as User or Administrator can verify the Mastercycler nexus, pro or ep. Overview The verification program checks the temperature accuracy of the thermoblock. The Mastercycler heats the thermoblock to 95 °C and then to 35 °C.
Temperature Verification System USB – Single-channel — Operating manual 1. Log in as User or Administrator. 2. Select System menu item under the cycler nodes. 3. Press the Verification softkey. A note on the Temperature Verification System USB – Single-channel will be shown. 4. Confirm the note with the OK softkey. You will be prompted to connect the Temperature Verification System USB – Single-channel. 5. Connect and switch on the Temperature Verification System USB – Single-channel.
Temperature Verification System USB – Single-channel — Operating manual 5.1.3 Verifying the Mastercycler ep Prerequisites • The Mastercycler is switched on. • The Measuring device is connected to the temperature sensor and switched on. Hint! Any Mastercycler ep connected to a control panel must be in the "Idle" state. No programs may be running. 1. Log in as User or Administrator. 2. Select System menu item under the cycler nodes. 3. Press the Verification softkey.
Temperature Verification System USB – Single-channel — Operating manual 13. Confirm the request with the OK softkey. The measuring cycle begins by tempering the thermoblock to 95 °C and 35 °C. 14. After each temperature control, read the temperature value of the measuring device and enter it in the control panel twice. Each additional measuring cycle begins with the request to insert the temperature sensor in a different bore. The position will be shown on the display. 15. Repeat steps 8 to 14.
Temperature Verification System USB – Single-channel — Operating manual 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. In the navigation tree, open the node of the cycler to be verified. Select the System menu item. Select the Verification tab. Start the verification using the icon. Select the Temperature Verification System USB – Single-channel using the checkbox. Use the icon to confirm the selection. You will be prompted to connect the Temperature Verification System USB – Single-channel.
Temperature Verification System USB – Single-channel — Operating manual 5.2 Adjusting the Mastercycler nexus/pro/ep Hint! 5.2.1 Only users who are logged in as Administrator can adjust the Mastercycler nexus, pro or ep. Overview The adjustment program checks the temperature accuracy of the thermoblock and adjusts the temperature measurement in the Mastercycler. The Mastercycler heats the thermoblock to 95 °C and then to 35 °C.
Temperature Verification System USB – Single-channel — Operating manual 1. Log in as Administrator. 2. Select the System menu item under the cycler node. 3. Press the Adjustment softkey. The adjustment program starts and heats the heated lid to 105 °C. You will then be prompted to insert the temperature sensor (Insert sensor in the position indicated). 4. Open the heated lid. 5. Insert the temperature sensor in the bore at the displayed position. 6. Close the heated lid. 7.
Temperature Verification System USB – Single-channel — Operating manual 5.2.3 Adjusting the Mastercycler ep Prerequisites • The Mastercycler is switched on. • The Measuring device is connected to the temperature sensor and switched on. 1. Log in as Administrator. 2. Select the System menu item under the cycler node. 3. Press the Adjustment softkey. A note on the Temperature Verification System USB – Single-channel will be shown. 4. Confirm the note with the OK softkey.
Temperature Verification System USB – Single-channel — Operating manual 14. After each temperature control, read the temperature value of the measuring device and enter it in the control panel twice. Each additional measuring cycle begins with the request to insert the temperature sensor in a different bore. The position will be shown on the display. 15. Repeat steps 8 to 14. The protocol will be shown on the display after the adjustment. The StoreMMC softkey is used to save the protocol.
Temperature Verification System USB – Single-channel — Operating manual 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. In the navigation tree, open the node of the cycler to be adjusted. Select the System menu item. Select the Adjustment tab. Start the adjustment using the icon. Select the Temperature Verification System USB – Single-channel using the checkbox. Use the icon to confirm the selection. You will be prompted to connect the Temperature Verification System USB – Single-channel.
Temperature Verification System USB – Single-channel — Operating manual 5.3 Verifying the Thermomixer and ThermoStat Prerequisites • The Thermomixer or ThermoStat is switched on. • The Measuring device with the temperature sensor is switched on. Do not verify the thermomixer during mixing. Hint! The temperature values of the Thermomixer or ThermoStat are not automatically compared to the temperature values of the measuring device. All values must be noted and manually evaluated after the measurement.
Temperature Verification System USB – Single-channel — Operating manual 6. Set a higher temperature on the device. The temperature is 75 °C on the ThermoStat and 90 °C on the Thermomixer. 7. Wait about 5 minutes after the display indicates that the set temperature has been reached. 8. Note the temperature displayed on the measuring device. 9. Calculate and note the difference to the temperature value on the device display. 10. Compare the noted temperature differences to the maximum permissible errors.
Temperature Verification System USB – Single-channel — Operating manual 7 7 7.1 Transport, storage and disposal Transport, storage and disposal Packing WARNING! Risk to health from contaminated device WARNING! 1. Observe the information in the decontamination certificate, You find it as a PDF file on our website (www.eppendorf.com/decontamination). 2. Decontaminate all the parts you would like to dispatch. 3. Include the fully completed decontamination certificate in the package.
Temperature Verification System USB – Single-channel — Operating manual 8 8 8.1 Maintenance Maintenance Servicing the Measuring device A description of the measuring device service can be found in the Measuring device operating manual. 8.2 Servicing the Temperature sensor Hint! Do not use organic solvents such as phenol, chloroform or acetone. The Temperature sensor does not require regular servicing. Follow these steps to remove contamination on the Temperature sensor: 1.
Temperature Verification System USB – Single-channel — Operating manual 1. Switch off the Temperature Verification System USB – Single-channel and remove it from the mains/power supply. 2. Let the Temperature sensor cool off. 3. Select a disinfection method that complies with the legal requirements and regulations in place for your range of application. 4. Spray the Temperature sensor with a disinfectant.
Temperature Verification System USB – Single-channel — Operating manual 9.3.2 Mastercycler Mastercycler family Temperature Error limits Mastercycler nexus 35 °C ±0,6 K 95 °C ±1,0 K 35 °C ±0,6 K 95 °C ±1,0 K 35 °C ±0,6 K 95 °C ±1,0 K Mastercycler ep Mastercycler pro 9.3.
Temperature Verification System USB – Single-channel — Operating manual 10 Ordering information 10 Ordering information Order no. (international) Description 0056 000.003 Temperature Verification System USB - Single channel For Mastercycler nexus, Mastercycler pro und Mastercycler ep, Thermomixer, ThermoStat 0056 001.000 0056 002.006 0056 003.
Temperature Verification System USB – Single-channel — Operating manual Index Index A Accuracy .................................................... 34 Adjustment Mastercycler ep .................................... 27 Mastercycler pro ................................... 25 Mastercycler with CycleManager pro28, 28 Mastercycler nexus............................... 25 C Certificate Temperature Verification System USB ... 9 Connection Mastercycler ep ....................................
Temperature Verification System USB – Single-channel — Operating manual Troubleshooting.................................... 31 O Ordering information ................................... 36 P Packing ....................................................... 32 Positioning the silicone mat Mastercycler ep 384 ............................. 18 Mastercycler pro 384 ............................ 18 Product description Temperature sensor 1,5 mL ................... 9 Temperature sensor 384 Well ................
Temperature Verification System USB – Single-channel — Operating manual 39
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