Operating manual Operating manual Eppendorf Research® plus Research plus - Operating Manual - en Operating manual
Copyright© 2010 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Trademarks Combitips®, eppendorf®, epT.I.P.S.®, Multipette®, Repeater® and Research® are registered trademarks of Eppendorf AG, Hamburg, Germany. Registered trademarks are not marked in all cases with ™ or ® in this manual. U.S. Pat. No.
Eppendorf Research® plus — Operating manual Table of contents Table of contents Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.3 Main illustration . . . . . . . . . .
Eppendorf Research® plus — Operating manual 1 Product description 1 1.1 Product description Features The Research plus pipettes are piston-stroke pipettes that operate according to the air-cushion principle. When the control button is pressed, the piston in the pipette moves in the same direction. If the control button and the piston are moved upwards, the liquid can be aspirated into the pipette tip. With the downward movement of the piston, the liquid is dispensed (measuring stroke).
Eppendorf Research® plus — Operating manual 1 Product description 1.3 1.3.1 Main illustration Research plus single-channel 1 2 1 Control button The control button and the trays of the matching epT.I.P.S. pipette tips have the same color. 2 Volume adjustment ring To set the volume for the variable pipettes. 4 3 Ejector The ejector moves the ejector sleeve and ejects the pipette tip. 5 6 7 8 Product description 3 1 4 Volume display (only variable pipettes) The set volume is read from top to bottom.
Eppendorf Research® plus — Operating manual 1 Product description 1.3.2 Research plus multi-channel For an explanation of the upper part of the pipette, refer to the main illustration of the single-channel pipette (see p. 5). 1 Product description 1 Lever To loosen the multi-channel lower part. 6 1 2 3 4 5 2 Multi-channel lower part The multi-channel lower part is freely rotatable. The lower part does not detach when it is rotated. The outer channels are labeled with the numbers 1 and 8 (or 12).
Eppendorf Research® plus — Operating manual 1 Product description 1.4 CAUTION! Materials CAUTION! Aggressive substances can damage the device, dispensing unit and accessories. Check for material compatibility before using organic solvents and aggressive chemicals. Follow the cleaning instructions (see Cleaning on p. 14).
Eppendorf Research® plus — Operating manual 2 Safety 2 2.1 Safety Intended use The Research plus is a lab device intended for dispensing liquids in the volume range from 0.1 μL to 10 mL, in combination with matching pipette tips. In vivo applications (applications in or on the human body) are not permitted. The Research plus may only be operated by trained specialist staff. All users must have read the operating manual carefully and familiarized themselves with the device's mode of operation. 2.
Eppendorf Research® plus — Operating manual 2 Safety NOTICE! NOTICE! NOTICE! Carry-over, contamination and incorrect dispensing results due to the incorrect use of pipette tips. The pipette tips are for single use only. Prolonged use can have a negative impact on dispensing tasks. Use the pipette tips only once. Do not autoclave the epT.I.P.S. Dualfilter. 2 Safety NOTICE! Incorrect dispensing volume for special liquids and from temperature differences.
Eppendorf Research® plus — Operating manual 3 Operation 3 3.1 Operation Setting the volume (only Research plus variable) Turn the volume setting ring as depicted to adjust the volume. The height of the control button changes as the volume is adjusted. The numbers on the volume display are to be read from the top to the bottom. The decimal places are below the hyphen. The volume is displayed up to 1 000 μL in μL. For the 5 mL and 10 mL sizes, the volume is displayed in mL.
Eppendorf Research® plus — Operating manual 3 Operation 3.3 Aspirating liquid 1. The liquid to be aspirated must be taken from a suitable vessel. For multi-channel pipettes, we recommend the reagent reservoir "Tip-Tub". 2. Press down the control button to the first stop (measuring stroke). 3. Immerse the pipette tip(s) vertically approx. 4 mm into the liquid. 4. To aspirate liquid, allow the control button to slide back slowly. Maintain the immersion depth, so that no air is aspirated accidentally. 5.
Eppendorf Research® plus — Operating manual 4 Troubleshooting 4 4.1 Troubleshooting Error search Symptom Liquid is dripping from the tip and/or the dispensed volume is incorrect. Possible cause Solution • The tip is loose or the Press the tip on firmly, use • Liquid with high vapor Wet the tip several times and • Pipetted too quickly. Move the control button slowly. pipette tip is poorly fitted. pressure and/or different density. epT.I.P.S. If using 5 mL and 10 mL epDualfilter T.I.P.S.
Eppendorf Research® plus — Operating manual 4 Troubleshooting To avoid dispensing errors, check the precision and accuracy of the Research plus at regular intervals. The "PICASO" program (from Version 2.3) is available to determine the permitted measured systematic and random errors. If the pipette is used at an extremely high location, it must be adjusted to the ambient air pressure. A SOP (Standard Operation Procedure) for checking pipettes can be found on the Research plus CD and on our website www.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Maintenance 5 Maintenance NOTICE! NOTICE! Aggressive substances can damage the pipette, pipette tips and the accessories. Check for material compatibility before using organic solvents and aggressive chemicals. Follow the cleaning instructions. • All single and multi-channel lower parts are wear parts. Clean them after contamination, use • of aggressive chemicals and/or heavy stress.
Eppendorf Research® plus — Operating manual 5 Maintenance 5.2 5.2.1 NOTICE! Damage to device from incorrect handling. Do not use any additional disinfectants, decontamination agents or sodium hypochlorite during autoclaving or UV exposure. When autoclaving make sure that the temperature does not exceed 121°C. Before using disinfecting agents or decontaminating agents, test for material compatibility and check the manufacturer's instructions about chemical resistance.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 5.2.2 Disinfection Carefully wipe off the outer surfaces with disinfectant, DNA/RNA decontamination agents or 70% isopropanol. Maintenance You can wipe the outside of the tip cone and the ejector sleeve with a sodium hypochlorite solution of 4%. After the exposure time of the sodium hypochlorite solution has elapsed, remove thoroughly with demineralized water.
Eppendorf Research® plus — Operating manual 5 Maintenance 5.4 5.4.1 5 Research plus disassembly and assembly Single-channel up to 1 000 μL Maintenance Removing the lower part PUSH UP TO RELEASE 1 2 3 4 1. Keep the ejector pressed and remove the ejector sleeve 1 . 2. 2 and 3 : on the lower part, slide the ring marked "PUSH UP TO RELEASE" up by about 5 mm until the lower part comes off. 3. 4 : take the lower part out of the upper part.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Opening and assembling the lower part (≤1 000 μL) To avoid confusion of parts, only disassemble and assemble one pipette at a time. Maintenance Hint! 1 2 3 4 Opening the lower part: 1. On the piston mount (1) push the locking mechanisms together slightly. 2. Remove the piston mount. 3. Remove the piston (2) and the piston spring (3). The piston is under spring tension.
Eppendorf Research® plus — Operating manual 5 Maintenance 5.4.2 5 5 mL and 10 mL single-channel Replace the protection filter in the tip cone after each contact with liquid or if it is frayed. Maintenance 4 1 30° 2 2 1 1. 2. 3 : unscrew the ejector sleeve. : keep the ejector pressed and turn the lower part to the left or right by approximately 30°. The lower part is automatically released from the upper part. 3.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Maintenance Before assembling the pipette, first install the piston mount, the piston spring and the piston in the upper part of the lower part. Further assembly is as shown in the figures. Carry out the procedure in reverse order. After the assembly: The assembly is performed in reverse order. After the assembly: Carry out a gravimetric test of the systematic and random error (see Technical data on p. 35).
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Removing and installing channels 1 1. 2. 3. 4. 5. 6. 2 3 4 Maintenance Channels must only be removed and installed if the multi-channel lower part is separated from the upper part! The channels in the lower parts consist of a piston, a cylinder and a spring. The channels for 100 μL and 300 μL are equipped with an O-ring at the tip cone (see Replacing O-rings on p. 16).
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Maintenance 5.5 NOTICE! Hint! 5.5.1 Adjusting pipettes NOTICE! Incorrect dispensing volume for special liquids and from temperature differences. Solutions which differ greatly from water in terms of their physical data, or temperature differences between the pipette, pipette tip and liquid can result in incorrect dispensing volumes. Avoid temperature differences between pipette, pipette tip and liquid.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Maintenance The density of distilled water is approx. 0.9982 mg/μL at 20°C and 0.9965 mg/μL at 27°C. If the set volume corresponds to the actual volume, no correction is necessary.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 5.5.2 Changing the user adjustment If the adjustment is changed, the volume changes by a certain value. Strictly speaking, the change only applies to the testing volume. Maintenance Auxiliary equipment • Supplied adjustment tool (order no. 3120 633.006) • Supplied red adjustment seal (ADJ) Example You readjust a 10 -100 μL pipette with a volume setting of 100 μL by 1 μL (1 μL 1%).
Eppendorf Research® plus — Operating manual 5 Maintenance 5.5.3 5 Changing the factory adjustment Maintenance Auxiliary equipment • Supplied safety plug tool • Supplied pin to loosen the safety plug It is possible to change the factory adjustment with the corresponding accessories for a Research plus with variable volume setting. If the factory adjustment of the Research plus has been changed by a user, this can be recognized by a red safety plug behind the ejector.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Maintenance 2. Mark the volume setting ring and the ejector with a common mark with a pen. This mark serves as an orientation for factory adjustment changes. When changing the factory adjustment, you can turn the volume setting ring, without the volume display changing. The mark on the volume setting ring and the ejector informs you how far you have moved from the factory setting. 3.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 4. Continue to keep the ejector pressed. Insert the safety plug tool such that the counter locking mechanism is pushed down. Maintenance 5. Turn the volume setting ring slightly to change the volume. Proceed as shown in the figure.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 This results in the following approximate volume changes: Single-channel Maintenance Nominal volume +½ revolution +¼ revolution –¼ revolution –½ revolution Color code 28 2.5 μL dark gray 0.106 μl 0.053 μl –0.053 μL –0.106 μL 10 μL medium gray 0.53 μl 0.27 μl –0.27 μL –0.53 μL 20 μL light gray 1.06 μl 0.53 μl –0.53 μL –1.06 μL 20 μL yellow 1.07 μl 0.54 μl –0.54 μL –1.07 μL 100 μL yellow 5.4 μl 2.7 μl –2.7 μL –5.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Multi-channel Nominal volume +½ revolution +¼ revolution –¼ revolution –½ revolution 10 μL medium gray 0.53 μl 0.27 μl –0.27 μL –0.53 μL 100 μL yellow 5.4 μl 2.7 μl –2.7 μL –5.4 μL 300 μL orange 10.7 μl 5.4 μl –5.4 μL –10.7 μL Maintenance Color code The values mentioned are theoretical values and are for orientation purposes only. The volume changes mentioned apply to each volume setting.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Maintenance 8. Insert the red safety plug on the tool into the opening of the Research plus and break it off from the tool. The red safety plug on the pipette indicates that the Research plus has been adjusted by the user. If the adjustment display had also been set to "0" before, you must close the opening with a new, red adjustment seal at the position for the adjustment seal. 9. Document the changes made and the measurements conducted.
Eppendorf Research® plus — Operating manual 5 Maintenance 5.5.5 5 Error limits in accordance with ISO 8655-2 Error limits in accordance with ISO 8655-2 - Research plus variable single-channel Test tip epT.I.P.S. Color code Testing volume 0.5 - 10 μL Increment: 0.01 μL 2 - 20 μL Increment: 0.02 μL 2 - 20 μL Increment: 0.02 μL 10 - 100 μL Increment: 0.01 μL 20 - 200 μL Increment: 0.02 μL medium gray 0.1 - 20 μL 40 mm light gray 0.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Error limits in accordance with ISO 8655-2 - Research plus variable single-channel Model Test tip Maintenance epT.I.P.S. Color code Testing volume 100 - 1 000 μL Increment: 1 μL 0.5 - 5 mL Increment: 5 μL 1 - 10 mL Increment: 10 μL 32 blue 50 - 1 000 μL 71 mm purple 0.1 - 5 mL 120 mm turquoise 1 – 10 ml 165 mm Random ±% ± μL ±% ± μL 30 μL ± 13.0 ± 3.9 ± 5.0 ± 1.5 150 μL ± 3.0 ± 3.9 ± 1.0 ± 1.5 300 μL ± 1.3 ± 3.9 ± 0.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 Error limits in accordance with ISO 8655-2 - Research plus variable multi-channel Model Test tip 10 - 100 μL Increment: 0.01 μL 30 - 300 μL Increment: 0.02 μL yellow 2 - 200 μL 53 mm orange 20 - 300 μL 55 mm Random ±% ± μL ±% ± μL 0.5 μL ± 48.0 ± 0.24 ± 32.0 ± 0.16 1 μL ± 24.0 ± 0.24 ± 16.0 ± 0.16 5 μL ± 4.8 ± 0.24 ± 3.2 ± 0.16 10 μL ± 2.4 ± 0.24 ± 1.6 ± 0.16 10 μL ± 16.0 ± 1.6 ± 6.0 ± 0.6 50 μL ± 3.2 ± 1.
Eppendorf Research® plus — Operating manual 5 Maintenance 5 5.6 Decontamination before shipment Maintenance If you wish to return the pipette to Eppendorf AG or an Eppendorf AG service partner to be checked or repaired, please note the following: CAUTION! CAUTION! Use of a contaminated device may result in personal injuries and damage to the device. Clean and decontaminate the Research plus before shipping or storage according to the cleaning instructions.
Eppendorf Research® plus — Operating manual 6 Technical data 6 6.1 Technical data Research plus single-channel variable Research plus variable single-channel Model Test tip epT.I.P.S. Color code Testing volume ± μL ±% ± μL 0.1 μL ± 48 ± 0.048 ± 12 ± 0.012 0.25 μL ± 12 ± 0.03 ±6 ± 0.015 1.25 μL ± 2.5 ± 0.031 ± 1.5 ± 0.019 2.5 μL ± 1.4 ± 0.035 ± 0.7 ± 0.018 2 - 20 μL Increment: 0.02 μL 2 - 20 μL Increment: 0.02 μL 10 - 100 μL Increment: 0.1 μL 20 - 200 μL Increment: 0.
Eppendorf Research® plus — Operating manual 6 Technical data Research plus variable single-channel Model Test tip epT.I.P.S. Color code 6 Testing volume Technical data 100 - 1 000 μL Increment: 1 μL 0.5 - 5 mL Increment: 0.005 mL 1 - 10 mL Increment: 0.01 mL 36 blue 50 - 1 000 μL 71 mm purple 0.1 - 5 mL 120 mm turquoise 1 – 10 ml 165 mm Random ±% ± μL ±% ± μL 30 μL ± 2.5 ± 0.75 ± 0.7 ± 0.21 150 μL ±1 ± 1.5 ± 0.3 ± 0.45 300 μL ± 0.6 ± 1.8 ± 0.2 ± 0.6 100 μL ±3 ±3 ± 0.6 ± 0.
Eppendorf Research® plus — Operating manual 6 Technical data 6.2 Research plus multi-channel variable Research plus variable multi-channel Model Test tip epT.I.P.S. Color code Testing volume ± μL ±% ± μL 0.5 μL ± 12 ± 0.06 ± 8.0 ± 0.04 1 μL ± 8.0 ± 0.08 ± 5.0 ± 0.05 5 μL ± 4.0 ± 0.2 ± 2.0 ± 0.1 10 μL ± 2.0 ± 0.2 ± 1.0 ± 0.1 30 – 300 μL Increment: 0.2 μL yellow 2 - 200 μL 53 mm 10 μL ± 3.0 ± 0.3 ± 2.0 ± 0.2 50 μL ± 1.0 ± 0.5 ± 0.8 ± 0.4 100 μL ± 0.8 ± 0.8 ± 0.
Eppendorf Research® plus — Operating manual 6 Technical data 6.3 Research plus fix Research plus fixed volume Model 6 Test tip Error limits epT.I.P.S. Color code Technical data Volume range Length Error Systematic Random ±% ± μL ±% ± μL 10 μL medium gray 0.1 - 20 μL 40 mm ± 1.2 ± 0.12 ± 0.6 ± 0.06 20 μL light gray 0.5 - 20 μL L 46 mm ± 0.8 ± 0.16 ± 0.3 ± 0.06 10 μL yellow 2 - 200 μL 53 mm ± 1.2 ± 0.12 ± 0.6 ± 0.06 ± 1.0 ± 0.2 ± 0.3 ± 0.06 25 μL ± 1.0 ± 0.25 ± 0.
Eppendorf Research® plus — Operating manual 6 Technical data 6.4 Ambient conditions Temperature range Relative humidity Storage without transport packaging –5 to 45°C 10 to 95% Operating conditions 5 to 40°C 10 to 95% 6 Technical data Technical specifications subject to change.
Eppendorf Research® plus — Operating manual 7 Ordering information 7 Ordering information The ordering information can be found on the Research plus CD.
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