Manual

19
If there is doubt that dispensing data are correct
To avoid dispensing errors, the precision and accuracy of the Research need to
be checked regularly. The PICASO II software program (see ordering information)
is available to determine permitted systematic and random measuring deviation
(see Section 2 "Technical data"). An SOP (
S tandard O perating P rocedure) for
checking pipettes can be called up from our home page
www.eppendorf.com .
For liquids whose physical data deviate significantly from those of water,
calibration needs to be changed in accordance with Section 5.2.3.
Calibration will also need to be changed if the pipette is being used at a very high
altitude.
The control
button jams
and does not
move smoothly.
– The piston is contaminated.
– The seal is contaminated.
– Solvent vapors have
entered the pipette.
– Clean the piston and
lubricate lightly (Part B,
"Maintenance").
– Remove the piston from the
pipette lower part (Part B,
"Maintenance"). Rinse out
the lower part with warm
water, then rinse with
distilled water and allow to
dry. Replace the seal if
necessary.
– Remove the piston from the
lower part (Part B,
"Maintenance") and aerate
the lower part. Clean and
lightly lubricate the piston.
Pipette
blocked; too
little liquid is
aspirated.
– Liquid has entered the nose
cone and dried.
– Remove the piston from the
pipette lower part (Part B,
"Maintenance"). Rinse out
the lower part with warm
water, then rinse with
distilled water and allow to
dry.
Error Cause Solution
Research – Part A – Section 7
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