Manual

18
Error Cause Solution
Droplets on the
inner wall of the
pipette tip.
– Uneven wetting of the
plastic wall.
– A pipette tip with poor
wetting properties has been
used.
– Attach a new pipette tip.
– Use an original Eppendorf
tip.
Pipette is
dripping and/or
the volume
pipetted is
incorrect.
– The tip is loose.
– A poorly fitting pipette tip
has been used.
– Liquid with a high vapor
pressure has been pipetted.
For 500–5000 µL/1 –10 mL
pipette:
– Pipetting was too fast.
– The tip was removed too
quickly from the liquid.
– The pipette is dripping
because:
piston is contaminated.
– Piston is damaged.
– Seal is damaged.
– The pipette lower part is
loose.
For multi-channel model:
– The O-ring of the channel is
damaged.
– Press the tip on firmly.
– Use an original Eppendorf
tip.
– In this case, we recommend
pipetting using a positive-
displacement pipette.
– Allow the control button to
slide back slowly.
– Pull the tip slowly out of the
liquid.
– Clean and lightly lubricate
the piston (Part B,
"Maintenance").
– Replace the piston and seal
(Part B, "Maintenance").
– Replace the seal;
with the 500–5000 µL and
1 –10 mL model, replace
the piston (Part B,
"Maintenance").
– Tighten the pipette lower
part by hand.
– Replace defective O-ring
(Part B, "Maintenance").
7 Troubleshooting
Research – Part A – Section 7
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