T_R_PatchMan_NP2.fm Seite 1 Donnerstag, 1.
T_R_PatchMan_NP2.fm Seite 2 Donnerstag, 1. Februar 2007 10:22 10 Inhalt / Contents / Sommaire / Indice / Indice Bedienungsanleitung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Inhalt / Contents / Sommaire / Indice / Indice Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Mode d’emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
00_Inhalt_e.fm Seite 48 Donnerstag, 1. Februar 2007 10:36 10 Contents Contents 48 1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 2 2.1 Safety precautions and application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Hazards when unpacking and other information and instructions . . . . . . . . . . . . . . 53 3 3.1 3.2 3.3 3.3.1 3.3.2 Description . . . . . . . . . . . . . . . . . . . . . . .
00_Inhalt_e.fm Seite 49 Donnerstag, 1. Februar 2007 10:36 10 Contents Turn wheel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Parameter setting via keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . General-Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Software version polling. . . . . . . . . . . . . . . . . . . . . . . . . . .
01_Ueberblick_e.fm Seite 50 Dienstag, 16. August 2005 3:13 15 1 Summary The Eppendorf PatchMan NP 2 micromanipulator is specially designed for use in electrophysiology, and is marked by extreme ease of operation. This makes the PatchMan NP 2 the right device for anyone performing electrophysiological measurements on cells and membrane pieces, as well as being suitable for use as a universal micromanipulator in many other applications.
02_Sicher_e.fm Seite 51 Dienstag, 16.
02_Sicher_e.fm Seite 52 Dienstag, 16. August 2005 3:15 15 • Caution! If the work is not carried out properly, for example if glass capillaries contact a surface, they may smash and flying splinters may endanger eyes. You should therefore always protect your eyes, such as by wearing goggles! 2 • Never point capillaries at persons! Capillaries can suddenly be loosened from their capillary grips under high pressure and become a missile.
02_Sicher_e.fm Seite 53 Dienstag, 16. August 2005 3:15 15 2 Safety precautions and application 2.1 Hazards when unpacking and other information and instructions Before checking that the device has been delivered complete and installing it, please read the following section thoroughly. The control board must not be carried or dragged by the joystick! The joystick is a highly sensitive control element which transmits movement commands in the 0.
03_Geraet_e.fm Seite 54 Dienstag, 16. August 2005 3:15 15 3 Description 3.1 Components The Eppendorf PatchMan NP 2 micromanipulator is made up of three components: – Control board – Module unit – Mains power supply unit Description 3 40° 20° 0 40° 20° Coa Fine rse Ext Fine ra Pos 1 Pa tc h Ma nN P2 Hom e Axi al Z on ly Sta nd by Fig. 1: PatchMan NP 2 micromanipulator, without mains power supply unit module unit for right side mounting 3.
03_Geraet_e.fm Seite 55 Dienstag, 16. August 2005 3:15 15 3 Description 1 3 Description 2 3 40° 20° 4 0 40° 20° Fig. 2: Module unit (here right side mounted) 1 2 3 4 Motor unit for Y and Z axis movement (Y/Z module) X module straight-line guide Motor unit for X axis movement (X module) X head with angle adjustment 3.3 Control board The control board accommodates the keypad and the joystick with integral button, as well as the turn wheel on the side.
03_Geraet_e.fm Seite 56 Dienstag, 16. August 2005 3:15 15 3 Description 3.3.1 Tool movement Moving the dynamic joysticks moves the capillaries in one plane. The speed of the motion is determined by the speed of the deflection movement on the joystick. Releasing the joystick stops the movement, the joystick springs back to its neutral position. Description 3 Rotating the joystick causes the tool to move up and down.
03_Geraet_e.fm Seite 57 Dienstag, 16. August 2005 3:15 15 3 Description Home Move out of working radius and return to the precise same spot. 3 Axial Activate or disable axial movement. Description Pos 1 Approach stored position. Store current position by pressing and holding down the key. Clear approached position by pressing and holding down the key. Z only Z only Only up and down movement (Z direction). Deactivates the X/Y movements. Standby Switches the device off or on.
04_Install_e.fm Seite 58 Dienstag, 16. August 2005 3:17 15 4 Installation 4.1 Unpacking Installation 4 – The packaging and the transport guards should be kept for use if the device is transported again later! – The control board must not be carried or dragged by the joystick! – The joystick is a highly sensitive control element which transmits movement commands in the 0.1 µm range.
04_Install_e.fm Seite 59 Dienstag, 16. August 2005 3:17 15 4 Installation 4.2 Assembling the module unit 4.2.1 Components of the module unit 4 1 Installation 2 40° 3 20° 0 4 40° 20° Fig. 3: Module unit (here right side mounted) 1 2 3 4 Motor unit for Y and Z axis movement (Y/Z module) X module straight-line guide Motor unit for X axis movement (X module) X head with angle adjustment 4.2.2 Straight-line guide The X module is assembled by bolting the straight-line guide onto the Y/Z module.
04_Install_e.fm Seite 60 Dienstag, 16. August 2005 3:17 15 4 Installation Moving the mounting point of the straight-line guide upwards changes the adjustable height. Moving the straight-line guide downwards allows less steeply inclined angles to be set for the tool. Installation 4 4.2.3 Assembling the X module Note: The orange fixing screw on the narrow front face of the X module must be slackened prior to adjusting the height and must then be re-tightened in order to ensure reliable operation.
04_Install_e.fm Seite 61 Dienstag, 16. August 2005 3:17 15 4 Installation 4.2.4 Assembling the X head Set the X head with the hole (2) at the tool mount (1) of the X module. The fitting pin must be fitted into the hole provided. Fix the X head to the X module by the M3x12 cheese head screw. 4 Installation 1 40° 2 20° 0 40° 20° 4.2.5 Assembling the hinged bracket Set up the hinged bracket according to the side on which the module unit is to be mounted.
04_Install_e.fm Seite 62 Dienstag, 16. August 2005 3:17 15 4 Installation Opening the hinged bracket 0° – 45° 4 Installation To open the hinged bracket beyond its normal opening angle between 0° and 45° for mounting purposes, push the pin on the hinge side up. The slider is purposely hard to move. The unit is usually only mounted once. The pin can be moved with the 4 mm Allen key. 0° – 45° 45° – 90° The hinged bracket can then be opened to 90°. The mounting holes on the module are then accessible.
04_Install_e.fm Seite 63 Dienstag, 16. August 2005 3:17 15 4 Installation Example: Motor module for left side mounting, rear. The hinged bracket is opened up and joined to the motor module by its inner inclined surface by means of the M5x16 screw. 4 Note: The X module is not shown. Installation Close the hinged bracket. Side view: The module unit for right side mounting is mounted on the hinge bracket. The hinge is not visible.
04_Install_e.fm Seite 64 Dienstag, 16. August 2005 3:17 15 4 Installation 4.3 Mounting the module on the microscope 4 The module unit mounting is dependent on the chosen side of the microscope. Installation 4.3.1 Right and left side mounting The module unit can be mounted on the most commonly used microscopes (usually inverted microscopes). The unit is mounted on the microscope tripod by way of a separately ordered microscope-specific adapter.
04_Install_e.fm Seite 65 Dienstag, 16. August 2005 3:17 15 4 Installation According to the mounting mode on the left side of the adapter. Note: The installation is carried out according to the planned mounting (section 5.4.3). 4 Installation 4.3.2 Assembling the capillary holder On the X module is the X head (1) for tool mounting. It is attached to the X module by one M3x12 screws as shown in the illustration.
04_Install_e.fm Seite 66 Dienstag, 16. August 2005 3:17 15 4 Installation To avoid having to reposition every time a capillary is changed, the positioning aid can be mounted on the universal capillary holder so it fits in the centre hole in the X head. Installation 4 Alternatively, the universal capillary holder can also be inserted in the lower mount on the X head. 40° 20° 0 40° 20° 4.3.3 Angle adjuster The desired angle can be set by turning one of the two knurled screws on the X head.
04_Install_e.fm Seite 67 Dienstag, 16. August 2005 3:17 15 4 Installation 4.3.4 Assembling the preamplifier holder The holder for the preamplifier is attached by two Phillips screws. The holder is mounted by way of the holes in its centre row. 4 The corresponding holes on the X head are revealed once the black knurled screw and the pressure disc have been removed. Installation To vary the position of the holder, it can be adjusted in its mounting holes.
04_Install_e.fm Seite 68 Dienstag, 16. August 2005 3:17 15 4 Installation 4.3.6 Position change in X direction Depending on the desired angle and/or the length of the tool, it may be necessary to remove the motor module further away from the working point. This change of position in X direction can be effected in two ways: 4 Installation 1.
04_Install_e.fm Seite 69 Dienstag, 16. August 2005 3:17 15 4 Installation 4.4 Connecting cables – Startup Note: The connections between the control board, the mains power supply unit and the motor module must only be plugged and unplugged with the device switched off.
05_Bedien_e.fm Seite 70 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation Section 5.1 provides a brief summary of how the commands can be used for micromanipulation. Section 5.2 provides additional information on the device's mode of operation. Sections 5.3 and 5.4 set out detailed descriptions of all the commands. 5 5.
05_Bedien_e.fm Seite 71 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation By storing a position, recurring operations at the same position can be made easier. To do this, move the capillary to the desired position and store the position by pressing and holding down the position key. Press and hold it down again to clear the position.
05_Bedien_e.fm Seite 72 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.2 More information on the mode of operation 5.2.1 Setting up the capillary – – 5 User control and mode of operation – Move the motor module to its top, outermost position. Mount the capillary with the capillary holder such that the tip of the capillary is approximately 20 mm above and 20 mm outside the working point. Adjust the height or the position in X direction as necessary.
05_Bedien_e.fm Seite 73 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.2.4 Capillary exchange Before swivelling it out, make sure the capillary cannot be impeded by any obstacles! Wear goggles to protect against possible glass splinters! Swivel out module unit on Y/Z module! 5 Swivel module unit for capillary exchange out of working radius. The capillary swivels out forwards and upwards with the module unit.
05_Bedien_e.fm Seite 74 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.3 Use of the keypad The keypad can be used to make settings or initiate movements directly. Frequently used settings can be entered by means of key combinations. The LEDs indicate the active condition of a function. 5 User control and mode of operation PatchMan NP 2 Coarse Fine Home Extra Fine Axial Pos 1 Z only Standby Some of the key functions are triggered when the keys are released. 5.3.
05_Bedien_e.fm Seite 75 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.3.3 Setting the speed The speed can be set in two ways: Directly using the turn wheel or by a long key press. Turn wheel 5 The turn wheel can be used to make speed settings directly. increase speed to maximum possible. Turn wheel backwards: reduce speed to minimum possible. User control and mode of operation Turn wheel backwards: The setting is made in stages.
05_Bedien_e.fm Seite 76 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.3.4 Home The tools moves axially out of the working radius. The function is intended for changing capillaries, or for quick removal of capillaries out of the working radius. 5 User control and mode of operation HOME: Press (and hold down as necessary) to move out as far as motors' end position. The red LED flashes during the movement, and is then lit steadily when the end position is reached.
05_Bedien_e.fm Seite 77 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.3.7 Z only Z only Only up and down movement (Z direction). Deactivates movement on the X and Y axes (right, left, forward and back), only movements in Z direction (up, down) are possible. When active the LED is lit. Deactivating enables the axes once again. The LED goes out. 5 User control and mode of operation 5.3.8 Standby / Reset Switches the device off or on. To switch on, press the "Standby" key again.
05_Bedien_e.fm Seite 78 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.4 Parameter setting via keys In addition to the speed setting (press and hold down "Speed" key) and position storage key functions, there are also other special functions operated via the keypad. 5 5.4.1 General-Reset User control and mode of operation The general reset resets the device to its delivery defaults. All the parameters, including the installation parameters, are reset to their factory defaults.
05_Bedien_e.fm Seite 79 Dienstag, 16. August 2005 3:18 15 5 User control and mode of operation 5.4.3 Installation The installation is carried out by means of keypad inputs. Execution: Coarse Fine – Device is on. Axial – Press "Speed" (Coarse/Fine) and "Axial" keys simultaneously. The Installation subfunction is activated and is indicated by the two LEDs on the "Speed" key being lit. Coarse 5 User control and mode of operation Fine – The "Home" key is used to enter right or left side mounting.
06_Wartung_e.fm Seite 80 Donnerstag, 1. Februar 2007 10:37 10 6 Cleaning and service 6.1 Cleaning Before cleaning the PatchMan NP 2, disconnect the plug. Ensure that no fluids enter into the inside of the micromanipulator in order to avoid short-circuits in the electrical installation as well as corrosion. 6 Care and maintenance Wipe painted parts and aluminum surfaces using a cloth and mild detergent and then with a dry cloth.
07_Fehler_e.fm Seite 81 Dienstag, 16. August 2005 3:19 15 7 Troubleshooting The following error situations are conceivable: Remedy – Check installation matches motor module setup, e.g. left side mounting etc. – Some user actions are not possible. – Perform a reset or general reset and then reinstall the device (section 5.4.3). – Device not responding to key presses, Home function still active. – Move joystick or press "Home" key again, tool moves downwards.
08_TechnDaten_e.fm Seite 82 Dienstag, 16. August 2005 3:21 15 8 Technical data Technical data 8 Power supply Input: Output: 120 – 240 V AC; 50 – 60 Hz; < 50 W 15 – 18 V DC; 25 W Control board Contamination level: Overvoltage category: Pollution degree: Ambient temperature: Ambient relative humidity: 15 – 18 V DC; 25 W II II (IEC 61010-1) 2 (IEC 664) max. 40 °C max. 80 % Weights Control board with power supply: Module unit: 2.7 kg 1.4 kg, incl.
09_Bestell_e.fm Seite 83 Donnerstag, 15. Juni 2006 9:35 09 9 Ordering information Delivery package 1 Control board 1 Motor module 1 Mains power supply unit 1 Mains power cable 1 X head with angle adjuster, incl.
09_Bestell_e.fm Seite 84 Donnerstag, 15. Juni 2006 9:35 09 9 Ordering information Accessories (non-standard) Universal capillary holder for accommodating Femtotips® II or grip heads Ordering information 9 Order no. Femtotips®, 5176 190.002 Grip head 0 for universal capillary holder for microcapillaries with an outer diameter of 1.0 to 1.1 mm 5176 210.003 Grip head 1 for universal capillary holder for microcapillaries with an outer diameter of 1.2 to 1.3 mm 5176 212.
09_Bestell_e.fm Seite 85 Donnerstag, 15. Juni 2006 9:35 09 9 Ordering information Order no. 25 VacuTips sterile glass capillaries for holding cells, inner diameter 15 µm, outer diameter 100 µm, 35° angle 5175 108.000 25 TransferTips®-F (ICSI) sterile glass capillaries for transferring sperm in the ICSI technique, inner diameter 4 µm, outer diameter 7 µm, with spike, 35° angle, flexible flange 5175 106.
09a_BestellUSA_e.fm Seite 86 Donnerstag, 15. Juni 2006 9:36 09 9a Ordering information for North America PatchMan NP 2, USA and Canada PatchMan NP 2, Europe PatchMan NP 2, UK/Hongkong PatchMan NP 2, Japan PatchMan NP 2, Australia PatchMan NP 2, China Ordering information for USA and Canada 9a Delivery package 1 Control board 1 Motor module 1 Mains power supply unit 1 Mains power cable 1 X head with angle adjuster, incl.
09a_BestellUSA_e.fm Seite 87 Donnerstag, 15. Juni 2006 9:36 09 9a Ordering information for North America Accessories (non-standard) Universal capillary holder for accommodating Femtotips® II or grip heads Order no. Femtotips®, 920007392 920007414 Grip head 1 for universal capillary holder for microcapillaries with an outer diameter of 1.2 to 1.3 mm 910012509 Grip head 2 for universal capillary holder for microcapillaries with an outer diameter of 1.4 to 1.
09a_BestellUSA_e.fm Seite 88 Donnerstag, 15. Juni 2006 9:36 09 9a Ordering information for North America Order no.
AnhangPC_e.fm Seite 89 Dienstag, 16. August 2005 3:25 15 A Appendix – PC interface A1 Overview It is easy to control the PatchMan NP 2 from a Windows®* computer if the following points are observed: Connection of the RS232 interfaces on the PC and the control panel using a zero modem cable. • The RS 232 data port must only be connected to devices conforming to the IEC 950/EN 60950 (UL1950) standards.
AnhangPC_e.fm Seite 90 Dienstag, 16. August 2005 3:25 15 A Appendix – PC interface The command and the parameters must be separated by separators. These can be either spaces or signs. Commas, etc. are not permissible as separators. If a space is used as a separator, the subsequent number is regarded as positive. With the exception of the block end marks, only displayable ASCII characters are used. The block end marks can be received as CR or LF or CR/LF. CR is always sent. All command/response codes (e.g.
AnhangPC_e.fm Seite 91 Dienstag, 16. August 2005 3:25 15 A Appendix – PC interface The control board device type and the version number of the software installed in the control board are returned as the reply. This command can be executed at any time. C002 General reset Parameter: None A002 Reset about to be performed Parameter: None The command is acknowledged and 100 ms later a general reset is performed. This command can only be executed when the remote control is operative.
AnhangPC_e.fm Seite 92 Dienstag, 16. August 2005 3:25 15 A Appendix – PC interface C008 STOP Parameter: None A008 All motors stopped Parameter: None A current movement is stopped. The checkback is sent as soon as all motors are stopped. This command can only be executed when the remote control is operative.
AnhangPC_e.fm Seite 93 Dienstag, 16. August 2005 3:25 15 A Appendix – PC interface C014 Parameters: Number of beep tones A014 All beep tones have been produced Parameters: None. The number of short (100 ms) beep tones that has been set in the parameter is produced. C015 Trigger short beep tones A Trigger long beep tones PC interface Parameters: Number of beep tones A015 All beep tones have been produced Parameters: None.
AnhangPC_e.fm Seite 94 Dienstag, 16. August 2005 3:25 15 A Appendix – PC interface A3 List of parameters A micrometer is equivalent to 25.6 micro-steps. One micro-step is equivalent to approx. 40 nanometers. px py pz Position of the x/y/z axes in micro-steps "–640000" ... "+640000" Px Py Pz Position of the x/y/z axes in micrometers "–25000" ... "+25000" vx vy vz Speed of the x/y/z axes in micro-steps per second "0" ... "192000" Vx Vy Vz Speed of the x/y/z axes in micrometers per second "0" ...
AnhangPC_e.fm Seite 95 Dienstag, 16. August 2005 3:25 15 A Appendix – PC interface Note: Error messages for software 1.00 – 1.
03_Fehler_sp.fm Seite 120 Donnerstag, 23.
T_R_PatchMan_NP2.fm Seite 3 Donnerstag, 1. Februar 2007 10:22 10 Eppendorf Offices ASEAN Eppendorf AG Regional Office in Malaysia Tel. +60 3 8023 2769 Fax +60 3 8023 3720 E-Mail: eppendorf@eppendorf.com.my FRANCE EPPENDORF FRANCE S.A.R.L. Tel. +33 1 30 15 67 40 Fax +33 1 30 15 67 45 E-Mail: eppendorf@eppendorf.fr Internet: www.eppendorf.fr SPAIN Eppendorf Ibérica S.L. Tel. +34 91 651 76 94 Fax +34 91 651 81 44 E-Mail: iberica@eppendorf.de Internet: www.eppendorf.es Internet: www.eppendorf.com.
In touch with life eppendorf is a registered trademark. B 5183 900.015-06/0807 · Printed in Germany T_R_PatchMan_NP2.fm Seite 4 Donnerstag, 23. August 2007 11:33 11 Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel. +49 40 538 01-0 · Fax +49 40 538 01-556 E-Mail: eppendorf@eppendorf.com Eppendorf North America, Inc. · One Cantiague Road, P.O. Box 1019 · Westbury, N.Y. 11590-0207 USA Tel.
