5353 900.
Copyright© 2007 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Trademarks eppendorf® and MixMate® are registered trademarks of Eppendorf AG, Hamburg, Germany. Trademarks are not marked in all cases with ™ or ® in this manual. 5353 900.
MixMate® — Operating manual EN Operating on see MixMate® p. Tab. Fig. —p. manual Operating manual Table of contents 1 Table of contents Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
MixMate® — Operating manual Operating manual EN 6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . .
MixMate® — Operating manual 1 EN Operating instructions 1 Operating instructions 1.1 Using this manual Operating manual Please read this operating manual completely before using the device for the first time. Also observe the instructions for use enclosed with the accessories. Please view this operating manual as part of the product and keep it somewhere easily accessible. When passing the device on to third parties, be sure to include this operating manual.
MixMate® — Operating manual EN 1.4 Abbreviations used Operating manual ANSI DNA DWP MTP PCR RNA rpm SBS 1.5 American National Standards Institute Deoxyribonucleic acid Deepwell plate Microplate Polymerase Chain Reaction Ribonucleic acid revolutions per minute Society for Biomolecular Screening Glossary Deepwell plate Plate with 48, 96 or 384 wells with a larger volume than microplates. Suitable for the preparation, mixing, centrifuging, transporting and storing of solid and liquid samples.
MixMate® — Operating manual 2 2 2.1 EN Product description Product description Main illustration Operating manual Abb. 1:Front and rear view Fig. 1: Front and rear view 1 Plate holder For holding skirted PCR plates, MTP and DWP as well as tube holders. 2 Vortex mat For direct vortexing of various tubes. 3 Softkeys For selecting preset mixing parameters. 4 Display Displays the mixing frequency and the mixing time (Fig. 4 on p. 12). 5 Operating controls Keys for operating the MixMate (Fig.
MixMate® — Operating manual EN Operating manual 2.2 Delivery package Quantity Order No. (International) Order No. (North America) 1 or 5353 000.014 5353 000.022 022674226 022674200 MixMate 230 V 120 V 1 1 1 5353 040.113 5353 040.121 5353 040.130 022674005 022674021 022674048 Tube Holder PCR 96 0.5 mL 1.5/2.0 mL 1 - - Power cable 1 5353 900.015 Operating Manual MixMate multi-lingual 1 5353 900.023 Short Instructions MixMate 2.
MixMate® — Operating manual 3.3 EN Information on product liability 3.4 Operating manual In the following cases, the designated protection of the device may be compromised. Liability for material damage and personal injury is transferred to the operator: • The device is not used in accordance with the operating manual. • The device is used outside of its intended use. • The device is used with accessories or consumables not recommended by Eppendorf.
MixMate® — Operating manual EN Operating manual WARNING! WARNING! WARNING! WARNING! NOTICE! NOTICE! NOTICE! 10 WARNING! Injury from flying tubes and plates. If the maximum permitted total weight of the mixing load is exceeded, plates or tubes may become detached from the device. Always ensure that tubes, plates and tube holder are well seated. The dimensions of the plates used must comply with the ANSI/SBS standards for microplates Mix the DWP and the tube holders 0.5 mL, 1.5/2.
MixMate® — Operating manual EN NOTICE! 4 NOTICE! Damage to electronic components due to condensation. After the device has been moved from a cool to a warmer environment, condensation may form inside the device. Wait at least three hours before connecting it to the power supply. Operating manual NOTICE! NOTICE! Damage from the use of aggressive chemicals.
MixMate® — Operating manual EN 5 5 Operating manual 5.1 Operation Operation Overview of operating controls Familiarize yourself with the operating controls and the display of the MixMate before using it for the first time. Abb. 3:Operator control elements and display Fig. 3: Operator control elements and display 1 Soft key for MTP (384-well) 3 Soft key for MTP (96-well) 5 Softkey for micro test tubes (1.5 and 2.
MixMate® — Operating manual 5.2 EN Inserting plates and tubes Operating manual WARNING! WARNING! Injury from flying tubes and plates. If the maximum permitted total weight of the mixing load is exceeded, plates or tubes may become detached from the device. Always ensure that tubes, plates and tube holder are well seated. The dimensions of the plates used must comply with the ANSI/SBS standards for microplates Mix the DWP and the tube holders 0.5 mL, 1.5/2.0 mL and PCR 96 with max. 2000 rpm.
MixMate® — Operating manual EN Operating manual 2. Then press the plate into the plate holder. In doing so, ensure that it is properly engaged. 5.2.2 Insert the tube holder in the plate holder 1. Select a suitable tube holder (see Tab. on p. 13). 2. Hold the tube holder up against the back edge of the plate holder so that the stop pins fit in the holes: 3. Engage the tube holder by pressing gently on the front. Remove the tube holder by pressing gently on the unlatching Push key to release the latch.
MixMate® — Operating manual 5.2.3 EN Insert the plate in the PCR 96 tube holder 5.2.4 Operating manual 1. Insert the PCR 96 tube holder in the plate holder (see Insert the tube holder in the plate holder on p. 14). 2. Push semi-skirted or unskirted PCR plates into the bores of the tube holder. In doing so, ensure they are evenly engaged. Insert tubes in the tube holders 1. Select the suitable tube holder (Tab. on p. 13). 2.
MixMate® — Operating manual EN Tab. 2: Softkeys for preset, optimized mixing parameters Operating manual Softkey Parameter Tube/ plate Fill level * 15 s/2000 rpm MTP and DWP (384 well) 10 to 60 % 15 s/2600 rpm PCR plates (384 well) 10 to 50 % 30 s/1000 rpm MTP (96 well) 5 to 60 % 30 s/1650 rpm PCR plates and DWP (96 5 to 50 % well), PCR tubes (0.2 mL) and micro test tubes (0.5 mL) 1 min/1400 rpm Micro test tubes (1.5 and 2.0 mL) 5 to 80 % * Amount of maximum filling volume.
MixMate® — Operating manual EN 5.4 Operating manual The remaining mixing duration and the current mixing frequency are displayed. At continuous operation the current mixing duration is displayed alternating with oo, after 99.5 h only oo is displayed. You can also change the parameters during a mixing process by pressing the time and speed arrow keys.To do so, the key lock may not be active (see Key lock (LOCK) activation/ deactivation on p. 20).
MixMate® — Operating manual EN 5.4.2 Using free parameters Operating manual You can also vortex on the vortex mat of the MixMate using free parameters. Vortexing time can be set variably from 15 s to infinite, vortexing frequency from 300 to 2000 rpm. Hint! In this Vortex mode, the TOO FAST message may be issued at frequencies > 2000 rpm. The MixMate automatically reduces the frequency to 1400 rpm. In the display the messages TOO FAST and 1400 rpm appear alternately.
MixMate® — Operating manual 5.5.1 EN Menu structure Abb. 5:Menu structure Operating manual Fig. 5: 5.5.2 Menu structure Menu navigation With the menu/enter button, you can switch between the menu levels or confirm the changed parameters. You can select the parameters with the arrow key 11. When M – 1 BACK or M – 2 BACK is displayed, press the menu/enter button to exit the menu level without saving changes.
MixMate® — Operating manual EN 5.5.3 Key lock (LOCK) activation/deactivation The key lock prevents set parameters being modified inadvertently during a mixing operation. Press the menu/enter button to call up the menu. Press the menu arrow key once. Press the menu/enter button to open the key lock menu. Select sub-item M – 2 ON with the menu arrow key to activate the key lock, or M – 2 OFF to deactivate the key lock. 5. Press the menu/enter button to confirm the selected setting.
MixMate® — Operating manual Symptom/ message Cause Remedy Too fast Mixing load not properly positioned in the plate holder. Check that the mixing load is Too fast Continuous vortexing at frequencies > 2000 rpm. Reduce the continuous ERR00 - ERR03 / ERR06 - ERR11 Electronics error Switch off the device and ERR04 - ERR05 Device overheats. The ventilation slots on the underside of the device are blocked. 1. Switch off the device and leave to cool down for 10 minutes. 2.
MixMate® — Operating manual Operating manual EN WARNING! NOTICE! NOTICE! NOTICE! WARNING! Device fire due to penetration of liquid Penetration of liquid can cause a fire due to a short-circuit in the device. Do not allow any liquids to penetrate the inside of the housing. Only mix in closed tubes and closed plates. If liquid has penetrated the inside of the housing: switch off the device, pull the power plug and have the device cleaned by service personnel authorized by Eppendorf.
MixMate® — Operating manual 7.2 WARNING! NOTICE! DANGER! Electric shock as a result of penetration of liquid. Switch off the device and disconnect it from the power supply before starting cleaning or disinfecting work. Do not allow any liquids to penetrate the inside of the housing. Do not spray clean/spray disinfect the housing. Only connect the device to the power supply if it is fully dried inside and out.
MixMate® — Operating manual EN 8 8 Operating manual 8.1 Transport, storage and disposal Transport, storage and disposal Transport Only transport the device in the original packaging. Air temperature Max. rel. humidity Air pressure General transportation -20 to 60 °C 10 to 95 % 30 to 106 kPa Air freight -20 to 55 °C 10 to 95 % 30 to 106 kPa Air temperature Max. rel.
MixMate® — Operating manual 9 9 9.1 EN Technical data Technical data Power supply 220 to 240 V ±10 %, 50 to 60 Hz 110 to 120 V ±10 %, 50 to 60 Hz Power consumption: 40 W Overvoltage category: II 9.2 Ambient conditions Environment: Use only indoors Ambient temperature: 2 to 40 °C Relative humidity: 10 to 75 % Atmospheric pressure: Use up to an altitude of 2000 m above MSL. Degree of contamination: 2 9.
MixMate® — Operating manual EN 10 Ordering Information Operating manual 10 Ordering Information CAUTION! CAUTION! Poor safety due to incorrect accessories and spare parts. The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, function and precision of the device.
MixMate® — Operating manual Order No. Description (North America) 0030 124.359 951010022 PCR Tube Strips 0.2 mL, colorless, 120 strips 951010006 PCR Tubes 0.2 mL per 1,000 pieces colorless 951020401 951020303 twin.tec PCR Plate 96 Wells colorless skirted, colorless, 25 pcs. semi-skirted, colorless, 25 pcs.
MixMate® — Operating manual EN Index Index Operating manual A Abbreviations ................................................ 6 Adjusting the volume .................................. 20 Application fields........................................... 8 Application parameters ............................... 25 Applications .................................................. 8 C Changing the parameters. Mixing ................................................... 17 Vortexing ...................................
MixMate® — Operating manual R Rear view...................................................... 7 Resuspending............................................... 6 S Safety Information......................................... 9 Select holder............................................... 13 Select location ............................................ 11 Semi-skirted.................................................. 6 Signal tone.................................................. 20 Skirted ......................
MixMate® — Operating manual Operating manual EN 30
Declaration of Conformity The device was tested in accordance with EN ISO 5349-1 : 2001 “Mechanical vibration – measurement and assessment of human exposure to hand-transmitted vibration – Part 1” Product name: MixMate 5353 Product type: Mixer for microtest tubes and plates with integrated touch vortex function Hand-arm-vibration assessment of MixMate 5353 in accordance with DIN ISO 5349-1:2001 Tests were performed on the MixMate operated in the “touch vortex” operation mode (3500 rpm) with 15 ml and 50 m
Exposure Limit Value (ELV) The table indicates that the operator using 15 ml Falcon tubes in touch vortex mode (3500 rpm) with reported usage would reach the ELV in 444 minutes and using 50 ml Falcon tubes in 136 minutes. Based on a usage pattern of 30 seconds vortex / 5 seconds gap, this means that approximately 761 of the 15 ml or 233 of the 50 ml Falcon tubes could produce exposures that reach the ELV. Immediate action must thus be taken to limit exposures to below the ELV.
* *) Sample measurement for this device only.
* *) Sample measurement for this device only.
* *) Sample measurement for this device only.
Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt.
