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Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of Eppendorf AG. Trademarks Eppendorf®, the eppendorf logo, Mastercycler®, SteadySlope®, vapoprotect® and Eppendorf twin.tec® are registered trademarks of Eppendorf AG. Registered trademarks are not marked with ® or ™ in all cases in this manual. Notice The software of the Mastercycler pro contains open source software. License information is available as PDF documents via the Eppendorf node.
Table of contents Mastercycler pro English (EN) Table of contents Operating Mastercycler on see p. Tab. Fig. p. manual pro English Operating (EN) manual 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Danger symbols and danger levels . . . . . . . . . . . . .
Table of contents Mastercycler pro English (EN) 5.5 Managing folders and programs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.5.1 Creating folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.5.2 Copying folders and programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.5.3 Deleting folders and programs . . . . . . . . . . . . . . . . . . . . . . . . .
Table of contents Mastercycler pro English (EN) 8.6 Performing a control panel software update. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.6.1 Performing an automatic software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.6.2 Performing a manual software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Transferring programs from the Mastercycler 533x. . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Table of contents Mastercycler pro English (EN)
Operating instructions Mastercycler pro English (EN) 1 1.1 Operating instructions Using this manual Read the operating manual before you operate the device for the first time. Please view this manual as part of the product and keep it somewhere easily accessible. If this manual is lost, please request another one. The current version of the operating manual can be found on our website at www.eppendorf.com.
Operating instructions Mastercycler pro English (EN) 1.3 Symbols used Depiction Meaning 1. 2. Actions in the specified order Actions without a specified order • List Text Display text or software text Additional information 1.
Product description Mastercycler pro English (EN) 2 2.1 Product description Main illustration Abb. 2-1: Mastercycler pro - front and rear view 1 2 34 5 6 7 8 11 9 10 Fig. 2-1: Mastercycler pro - front and rear view 1 Lid handle For opening and closing and also locking and unlocking the heated lid. 2 vapo.protect heated lid 3 Address display 4 Status lamp 5 Rear plate with ventilation slots 6 Mains connection socket 7 Mains switch 0 = switched off, I = switched on.
Product description Mastercycler pro English (EN) Abb. 2-2: Terminal block Fig. 2-2: Printer RS 232 Control Panel 1 2 3 CAN_out CAN_in Term Address 4 5 6 7 Terminal block 1 Parallel interface Connection for printer. 5 CAN in Data inlet for connecting several Mastercycler pro or Mastercycler ep. 2 Serial interface (RS 232) Connection for Eppendorf temperature validation 6 Term switch Termination of CAN bus when networking several system. Mastercycler pro or Mastercycler ep.
Product description Mastercycler pro English (EN) 2.2 Delivery package The contents of the delivery package depend on the variant of your Mastercycler pro. 2.2.1 Mastercycler pro and Mastercycler pro S Quantit Order no. y (International) 1 or 6321 000.019 6321 000.027 1 or 6325 000.013 6325 000.021 1 6321 900.150 1 - 1 - Order no.
Product description Mastercycler pro English (EN) 2.3 Features Product variants The Mastercycler pro is available in three variants. • Mastercycler pro • Mastercycler pro S • Mastercycler pro 384 All variants are provided with the following features: Triple Circuit Technology All Mastercycler pro thermomodules feature extremely fast heating and cooling rates.
Product description Mastercycler pro English (EN) 2.3.1 Features of the Mastercycler pro variants Variant Features Mastercycler pro • • • • Mastercycler pro S • Highest temperature control speed due to silver block: up to 6°C/s. • Standard PCR possible in under 30 min. • Pulse function for device-driven hot start for avoiding non-specific PCR product formation. • Freely programmable temperature gradient over 12 rows. • Extended gradient range of up to 24°C. • Holds up to 96 PCR tubes (0.
Product description Mastercycler pro English (EN)
Safety Mastercycler pro English (EN) 3 3.1 Safety Intended use The Mastercycler pro is intended to be used in research and routine laboratories in the field of biomedical sciences. The Mastercycler pro is used to control the temperature of aqueous solutions, suspensions, and emulsions in closed micro test tubes for enzymatic reactions, typically for polymerase chain reaction (PCR). The Mastercycler pro is intended exclusively for indoor use. 3.
Safety Mastercycler pro English (EN) DANGER! Risk when handling toxic or radioactively-marked liquids or pathogenic germs. Follow national regulations governing the handling of these substances. For complete instructions regarding the handling of germs or biological material of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).
Safety Mastercycler pro English (EN) WARNING! Burns from hot thermoblock and heated lid. The thermoblock, the inner side of the heated lid and micro test tubes/PCR plates can reach temperatures of over 50 °C very quickly. The heated lid should be kept closed until temperatures of 30 °C or less are reached. Take note of the symbols on the thermoblock warning that surfaces may be hot.
Safety Mastercycler pro English (EN) WARNING! Risk of injury when lifting and transporting the device. Lifting the device with the wrong posture may cause serious damage to your health. If the device falls to the ground it can cause injuries. Do not lift the device by the handle or the housing of the heated lid. Carry the device securely with both hands to prevent it from slipping. Always keep a straight back when lifting and carrying the device.
Safety Mastercycler pro English (EN) CAUTION! Lack of safety due to missing operating manual. When passing on the device, always enclose the operating manual. If you lose the operating manual, request a replacement. The current version of the operating manual and the safety instructions can also be found on our websitewww.eppendorf.com. 3.5 Warning signs on the device This section explains the warning signs used on the Mastercycler pro.
Safety Mastercycler pro English (EN)
Installation Mastercycler pro English (EN) 4 4.1 Installation Unpacking the device WARNING! Risk of injury when lifting and transporting the device. Lifting the device with the wrong posture may cause serious damage to your health. If the device falls to the ground it can cause injuries. Do not lift the device by the handle or the housing of the heated lid. Carry the device securely with both hands to prevent it from slipping. Always keep a straight back when lifting and carrying the device. 1.
Installation Mastercycler pro English (EN) 4.2 Selecting the location 4.2.1 Space requirements and bearing capacity CAUTION! Material damage from uneven work surface. Position the device on level and stable work surface which is capable of bearing the weight of the device. The device must not be placed on a trolley or at an angle. CAUTION! Damage due to overheating. Do not place the device close to sources of heat (e.g. radiator, drying cabinet). Do not expose the device to direct sunlight.
Installation Mastercycler pro English (EN) 4.3 Installing the instrument This section describes how to start up the Mastercycler pro with the control panel and how to operate several devices belonging to the Mastercycler pro and Mastercycler ep series with each other in a network. The connections and switches are located at the back of the device (see Main illustration on p. 9). • Follow the steps below to define the settings and connect the devices before switching on the Mastercycler pro.
Installation Mastercycler pro English (EN) 1. Connect the cable on the control panel to the "Control Panel" connector of the first device. CAN_out CAN_in 2. Connect the CAN bus cable to the "CAN_out" connector of the first device. 3. Connect the free end of the CAN bus cable to the "CAN_in" connector of the second device. Connecting additional devices 4. Always use the CAN bus cable to connect the "CAN_out" connector of the last connected device to the "CAN_in" connector of the new device.
Installation Mastercycler pro English (EN) 4.3.3 Connecting Mastercycler pro to an existing Mastercycler ep You can extend an existing system of one or more Mastercycler ep devices with a corresponding control panel using devices of the Mastercycler pro series. When operating the Mastercycler pro with a Mastercycler ep control panel (without USB connections), the following restrictions apply: • The self test function is not available.
Installation Mastercycler pro English (EN) 4.3.5 Connecting the printer The Mastercycler pro can be connected to a parallel interface printer in order to print protocols and programs in text format. Connect the printer via a parallel data cable to the parallel interface (see Fig. 2-2 on p. 10) of the Mastercycler pro. A printer with USB interface can be connected to the control panel to print protocols, programs and self test certificates.
Operation Mastercycler pro English (EN) 5 5.1 Operation Initial steps Check the correct installation Before the Mastercycler pro is started up for the first time, ensure the following: • The device is correctly connected. • The device is free of damage. • Make sure that air can circulate freely around the ventilation slots on the rear of the device and around the fan behind the feet. 5.1.1 Using the vapo.protect heated lid Thevapo.protectheated lid features a heated flexible pressure unit.
Operation Mastercycler pro English (EN) 1 2 1. Pull vapo.protect heated lid forwards at the lid handle as far as possible so that the lid handle can be rotated. 2. Flip the handle over the heated lid until it clicks into place. You can now start a program run or manually temperature-control the lid and the samples. 5.1.1.2 Opening the heated lid WARNING! Risk to health and bio hazard when operated with lid open. The tube lids may pop open at high block temperatures.
Operation Mastercycler pro English (EN) 5.1.1.3 Status lamp The vapo.protect heated lid is equipped with a status lamp 2(see Main illustration on p. 9) which allows the current operating status of the Mastercycler pro to be visible from far off. Status lamp Operating status Illuminates green The Mastercycler pro is idle. Flashes green A program run is active. Flashes orange The program run is waiting for user intervention in a pause or hold step(see Editing programs on p. 44).
Operation Mastercycler pro English (EN) 5.1.2 Control panel keys 1 Stop 4 ghi 7 Start 2 3 abc def 5 6 jkl 8 pqrs tuv 0 . mno 9 wxyz Delete Enter Next Start the selected program. Start Stop the running program. Stop Function keys The function of the key changes with the software dialog and appears in the display immediately above the key. • Step-by-step navigation. The current position of the cursor is marked. • Move the cursor within an alphanumerical input field.
Operation Mastercycler pro English (EN) 5.2 Initial start-up of the Mastercycler pro During the initial startup of the control panel you can define an administrator PIN in order to protect the system against access by unauthorized persons. These data are saved in the control panel and are not lost when the Mastercycler pro is switched off. 5.2.1 Defining the administrator PIN If no administrator PIN has been defined, you are prompted to do so.
Operation Mastercycler pro English (EN) To define the administrator PIN, perform the following steps in the sequence described. 1. Switch on the Mastercycler pro at the mains power switch. The Input Admin PIN window appears. 1 4 ghi 7 2 abc 5 jkl 8 pqrs tuv 0 . 2. Enter the desired administrator PIN using the numerical keys on the control panel. The PIN can be between 4 and 8 digits long. 3 def 6 mno 9 wxyz Delete 3. Go to the Confirmation field and enter the PIN again to confirm.
Operation Mastercycler pro English (EN) 5.3 User login and logout The functions described here are only available if the PIN function is activated. 5.3.1 Logging in as a user Before you can log in under your user name, your administrator must have set up a user account for you. Perform the following steps in the sequence described. 1. Switch on the Mastercycler pro at the mains power switch. The control panel switches on automatically and the software is loaded.
Operation Mastercycler pro English (EN) 1. Mark your user node. 2. Press the Login function key. 3. Log in as a user. The user who was previously logged on is automatically logged out. 5.3.3 Logging out as a user You can log yourself out from the control panel to protect your programs against unauthorized changes. 1. Mark your user node. 2. Press the Logout function key. You are now logged out. The guest user is automatically logged in.
Operation Mastercycler pro English (EN) 5.4 Overview of operation with the control panel 5.4.1 Navigation tree overview 1 2 3 4 5 6 4 Cycler node 1 Window title Each cycler that is connected and switched on is Displays the current level (user level, cycler level, displayed by a separate cycler node. Here you top level) in the navigation tree and the title of can open the status view of the respective cycler dialog windows.
Operation Mastercycler pro English (EN) Abb. 5-1: Navigation tree of the Mastercycler pro. The MMC and USB nodes are only visible if a storage medium has been inserted. Fig. 5-1: Navigation tree of the Mastercycler pro. The MMC and USB nodes are only visible if a storage medium has been inserted. 5.4.2 Using the navigation tree 5.4.2.1 Opening the node You have three options: Mark the node with the arrow keys and press the Enter key or the arrow key .
Operation Mastercycler pro English (EN) 5.4.2.2 Closing the node Carry out the above-mentioned steps in the reverse order: Use the mouse to click on the minus symbol in front of the node. Alternatively, double-click on the node. Or mark the node with the arrow keys and press the Enter key or the arrow key . This closes the node and the minus symbol in front of the node changes to a plus symbol. 5.4.
Operation Mastercycler pro English (EN) 5.4.3.2 Removing storage media CAUTION! Device damage due to cancellation of the update process. Ensure power supply during the update. Do not switch the cycler off during the update. Do not disconnect the control panel from the cycler during the update. Leave the external storage medium in the control panel until the latter has been restarted after the update and you have logged on again.
Operation Mastercycler pro English (EN) 5.5 Managing folders and programs If you are logged in as a normal user (not as the administrator), you are only authorized to create, edit and delete programs and folders below your own user node. More information on user rights can be found in a separate section(see Administrator functions on p. 55). 5.5.1 Creating folders and programs 5.5.1.1 Creating folders Proceed as follows: 1. Mark the user node. 2. Press the New Folder function key. 3.
Operation Mastercycler pro English (EN) The New Cycler Program window is displayed. You can enter a name and a comment for the new program using the numerical keys here. 3. Press the OK function key. The program is created and appears under your user node. You can change the name of the program or the comment at any time using the Prop. function key. To protect a program against inadvertent changes, you can use the Prop. function key to mark it as write-protected (read only).
Programming Mastercycler pro English (EN) 6 6.1 Programming Overview of the program editor The graphic program editor can be used to view and change existing programs. 6.1.1 Opening the program editor 1. Mark the desired program in the navigation tree or create a new program (see Creating a new program on p. 39). 2. Press the Edit function key. The program editor is displayed. 6.1.
Programming Mastercycler pro English (EN) 6.2 Defining general settings Each Mastercycler pro program features a program header in which you can define the block temperature control mode and heated lid behavior. These settings are valid for the entire program. 6.2.1 Opening the header 1. Open the program editor (see Opening the program editor on p. 41). 2. Press the Header function key. The header settings are displayed. 6.2.
Programming Mastercycler pro English (EN) TSP/ESP heated lid Preheat heated lid • Mastercycler pro: Switch TSP on and off. When TSP is activated, the heated lid is heated to the set temperature, while the block is maintained constant at 20 °C. The program is started after the lid temperature has been reached. • Mastercycler ep: Switch ESP on and off. When ESP is activated, the heated lid is first heated to the set temperature before it is lowered onto the tube surfaces.
Programming Mastercycler pro English (EN) 6.3 Editing programs You can add, edit or delete steps in new (see p. 39) or copied (see p. 40) programs. 6.3.1 Inserting a program step 1. Program editor(see Opening the program editor on p. 41) . 2. Use the arrow keys to mark the program step before which the new program step is to be inserted. The marked program step is highlighted in blue. 3. Press the Insert function key. A list appears containing the available program steps. 4.
Programming Mastercycler pro English (EN) 6.3.2 Editing parameters 1. Use the arrow keys to mark the desired program step. The marked program step is highlighted in blue. You can make the settings for temperature, hold time and number of repetitions directly in this view. Changing the temperature 2. Use the arrow keys to mark the temperature. The temperature is highlighted in blue. 3. Use the numerical keys to enter a new value between 4.0 °C and 99.0 °C (in increments of 0.1 °C). 4.
Programming Mastercycler pro English (EN) Temp Temperature for the program step. Equivalent to directly editing in the program view. Temp Inc./Dec. For changing the temperature to the next cycle. Values ranging from -10.0 °C to +10.0 °C in increments of 0.1 °C are possible. You can switch between positive and negative values by pressing the+/- function key. Gradient Column temperature gradient in the thermoblock.
Programming Mastercycler pro English (EN) 6.3.4 Deleting all program steps 1. Press the ClearAll function key. A prompt appears asking whether you want to delete all the program steps. 2. Press the OK function key. All program steps are deleted. 6.3.5 Exporting a program listing You can export a program to an external storage medium as a file for documentation purposes. 1. Insert the external storage medium into the control panel. 2. Press the >> function key. 3. Press the Export function key. 4.
Programming Mastercycler pro English (EN)
PCR run Mastercycler pro English (EN) 7 7.1 PCR run Preparation 7.1.1 Selecting the sample tubes CAUTION! Unsuitable tubes can result in sample material escaping. Unsuitable tubes may be so severely damaged that sample material is released. Use only tubes whose properties meet at least the requirements specified in these operating instructions. This is especially important when working with infectious material. CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.
PCR run Mastercycler pro English (EN) 7.1.2 Loading the thermoblock CAUTION! Damage to the thermoblock from incorrect operation. Do not fill sample material directly into the thermoblock. 7.1.2.1 Loading a 96 well block The Mastercycler pro and Mastercycler pro S can be loaded with 0.2 mL single tubes or strips. You can also use a 96-well PCR plate.
PCR run Mastercycler pro English (EN) 7.2 Starting the program Start the PCR programs from the editor or the navigation tree. If the PIN function is activated, you can only start programs which are stored below your user node (see p. 55). Prerequisites You are logged in as user and the program to be started is saved. 1. Mark the program in the navigation tree with the arrow keys or open it in the editor. 2. Insert the sample tubes (see Loading the thermoblock on p. 50). 3.
PCR run Mastercycler pro English (EN) 7.2.1 Status view The status view is automatically displayed immediately after a program is started. It shows the progress of and information on a running program. The currently active step is displayed as a flashing line.
PCR run Mastercycler pro English (EN) 7.3 Interrupting or stopping the program You can temporarily interrupt the program run in the status view, for instance, to add reagent, or completely stop the program. 7.3.1 Interrupting the program 1. Open status view (see Starting the program on p. 51). 2. Press the Stop key. The status view shows Paused. Observe the safety precautions for opening the heated lid (see Opening the heated lid on p. 28). 7.3.2 Continuing the program 1.
PCR run Mastercycler pro English (EN) The protocol is displayed. 3. Use the function keys << and >> to go one page back or forward. Use the arrow keys on the control panel to move the visible section. Further functions for saving and printing the protocol are available in the protocol screen. Saving a protocol Printing a protocol 1. Insert a USB storage medium or an MMC . 2. Press the function key Export. 3. Select Text or PDF file format. The protocol is copied to the external storage medium. 1.
System management Mastercycler pro English (EN) 8 8.1 System management Administrator functions The software of the Mastercycler pro differentiates between standard users and the administrator.
System management Mastercycler pro English (EN) 3. Enter the name and PIN for the new user. Repeat the PIN entry in the Confirmation field. 4. Confirm by pressing the OK function key. The new user account is set up. In the navigation tree, a new user node is displayed with this user name. The user can now log in with his or her PIN at any time. 8.2.
System management Mastercycler pro English (EN) 8.2.4.1 Changing the administrator PIN If you are logged on as an administrator, you can change the administrator PIN at any time. CAUTION! Loss of data due to misuse or loss of administrator PIN. The administrator PIN protects the system from undesired access to its configuration and to the stored data of all users. Make a note of the administrator PIN and keep it in a safe place. If you lose the administrator PIN, contact Eppendorf Service.
System management Mastercycler pro English (EN) 8.3 System settings Open the System node to set the following system settings. Show free system memory Mark the node System memory and press the function key Show. The system memory is shown as a red-green marking at the lower end of the display (red for the used memory, green for free memory). Press the Show function key again to hide the display. Exporting the log file Mark the Logfile node and press the Export or the Export 20 function key.
System management Mastercycler pro English (EN) Performing a software synchronization Use the update node to perform a software synchronization between the cycler and the control panel. The software synchronization may be necessary if you receive error messages after updating the software of the control panel. 1. 2. 3. 4. Highlight Update node. Press Cycler function key. Select the Cycler. Press Start.
System management Mastercycler pro English (EN) 8.4 Cycler functions In the Cycler node, you can view and adjust the status of the thermoblock and the heated lid. You can also retrieve the protocol of the last PCR program used. Prop. Display device properties. Incubate Control the thermoblock temperature (4.0°C to 99.0°C). Lid Control the heated lid temperature (37°C to 110°C). Status Switch to status view while the program is running.
System management Mastercycler pro English (EN) 8.4.2 Self test The self test function of the Mastercycler pro allows you to perform a quick check regarding the following attributes of the thermoblock without requiring an external measurement system: • Heating and cooling rates • Proper functioning of the temperature control circuit • Temperature homogeneity in each of the three zones of the block and across the entire block After a successful self test, a certificate can be generated in PDF format.
System management Mastercycler pro English (EN) Backup Back up user nodes, folders and programs onto an external storage medium. • MMC: The MMC inserted will automatically be completely deleted at the start of the backup process. • USB: Any files and folders in the directory \eppendorf will be deleted automatically at the start of the backup process. The backed-up data can be copied into the control panel using the Restore function. Restore 8.5.
System management Mastercycler pro English (EN) 8.6 Performing a control panel software update CAUTION! Device damage due to cancellation of the update process. Ensure power supply during the update. Do not switch the cycler off during the update. Do not disconnect the control panel from the cycler during the update. Leave the external storage medium in the control panel until the latter has been restarted after the update and you have logged on again.
System management Mastercycler pro English (EN) 3. Select Software Update Control Panel entry and press the Start function key. The software version currently installed and the software version on the storage medium are displayed. 4. Press the Update function key to start the update. You can cancel the operation using the Restart function key. The update may take several minutes. Afterwards, the control panel will automatically restart. 8.
System management Mastercycler pro English (EN) Make the settings in the header(see Editing header settings on p. 42). Setting from the Mastercycler 533x program Corresponding setting in the program header LID 105 °C Lidtemp 105 °C Wait TSP/ESP heated lid AUTO Switch off lid at low block temperature CNTRL BLOCK =Temp. mode block TUBE = Temp. mode safe orfast Block temperature control behavior Activate theSimulate Mastercycler gradient checkbox Press the OK function key.
System management Mastercycler pro English (EN)
Quick start Mastercycler pro English (EN) 9 9.1 Quick start Login 1. Open list. Enter 2. Select user. 3. Confirm selection. Enter 4. Go to the PIN field. 1 4 2 abc 5 ghi jkl 7 8 pqrs tuv 0 . Enter 3 def 6 mno 9 wxyz Delete 5. Enter and confirm PIN.
Quick start Mastercycler pro English (EN) 9.2 Creating folders and programs 1. Select the user or folder. 2. Press NewFolder orNew. 1 4 2 abc 5 ghi jkl 7 8 pqrs tuv 0 . 3 def 6 3. Enter and confirm names and comments. mno 9 wxyz Delete Enter 9.3 Copying folders and programs 1. Select the folder or program. 2.Press the Copy function key. 3. Confirm selection. Enter 4. Mark the target folder or user. 5. Press Paste.
Quick start Mastercycler pro English (EN) 9.4 Deleting folders and programs 1. Mark the folder or program. 2. Press the Delete key. Delete 3. Confirm the selection. Enter 9.5 Editing programs 1. Select the program. 2. Open the program editor. Enter 3.Press the Header function key. 6. Set the lid functions and control mode. 7. Confirm input.
Quick start Mastercycler pro English (EN) 8. Press the Insert function key. 9. Select the program step. 1 4 2 abc 5 ghi jkl 7 8 pqrs tuv 0 . 3 10. Enter the temperatures, hold times and cycle number. 11. Select the program step. 12. Press the Options function key. def 6 mno 9 wxyz Delete 13. Set the gradient, increment and ramp. 1 4 2 3 abc def 5 6 ghi jkl 7 8 pqrs tuv 0 . mno 9 wxyz Delete 14. Confirm input. Enter 15. Press the >> function key. 16.
Quick start Mastercycler pro English (EN) 9.6 Stopping and starting programs 1. Mark the program. Start 2. Insert the sample tubes. 3. Close the heated lid. 4. Start the program. 5. With several connected cyclers: Mark the cycler. 6. Confirm input. Enter 7. You can use the status view to carry out the following program commands • Stop • Abort • Resume 9.7 Switching off the device 1. Mark the Eppendorf or System node. 2. Press the Shutdown function key. 3.
Quick start Mastercycler pro English (EN)
Maintenance Mastercycler pro English (EN) 10 10.1 Maintenance Cleaning DANGER! Electric shock. Switch off the device and disconnect the power plug before opening the device, performing work on electrical connections or starting maintenance or cleaning work. WARNING! Burns from hot thermoblock and heated lid. The thermoblock, the inner side of the heated lid and micro test tubes/PCR plates can reach temperatures of over 50 °C very quickly.
Maintenance Mastercycler pro English (EN) CAUTION! Caution when using aggressive chemicals. Aggressive chemicals may damage both the device and its accessories. Do not use any aggressive chemicals on the device and accessories such as strong or weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol. If the device becomes contaminated with aggressive chemicals, clean it immediately with a neutral cleaning agent.
Maintenance Mastercycler pro English (EN) 1 2 3 4 1 Lid release latches 3 Rollflex cable 2 Heating unit 4 Stand 4. Remove any contamination in the area of the block and on the inner side of the lid with a damp, lint-free cloth. Use a neutral lab cleaner if necessary. Remains of markings from tube lids on the heating unit can be removed with ethanol or isopropanol. Keep the block bores free of dust and lint.
Maintenance Mastercycler pro English (EN) 10.2 Disinfection/decontamination DANGER! Electric shock as a result of penetration of liquid. Switch off the device and disconnect it from the power supply before starting cleaning or disinfecting. Do not allow any liquids to enter the inside of the housing. Do not perform spay disinfection. Only reconnect the device to the power supply once it is completely dry. CAUTION! Material damage from UV and other high-energy radiation.
Troubleshooting Mastercycler pro English (EN) 11 11.1 Troubleshooting General errors Technical faults may be triggered by faults such as a power failure or power fluctuations. In most cases it is therefore sufficient simply to switch the device off and back on again after about 10 seconds. If the fault occurs again, please note the fault code (e.g. 0x0B04) and inform Eppendorf Service. 11.
Troubleshooting Mastercycler pro English (EN)
Transport, storage and disposal Mastercycler pro English (EN) 12 12.1 Transport, storage and disposal Packing WARNING! Risk of injury when lifting and transporting the device. Lifting the device with the wrong posture may cause serious damage to your health. If the device falls to the ground it can cause injuries. Do not lift the device by the handle or the housing of the heated lid. Carry the device securely with both hands to prevent it from slipping.
Transport, storage and disposal Mastercycler pro English (EN) Proceed as follows: 1. Pay attention to the decontamination notes (see Decontamination before shipment on p. 76). 2. Switch off the device and open the heated lid. 3. Let the device stand for 30 minutes and ensure that there is no condensate in the thermoblock. 4. Disconnect the device from the power supply. 5. Disconnect all the connections from the rear of the device and remove the control panel. 6.
Transport, storage and disposal Mastercycler pro English (EN) 12.3 Disposal In the event of disposing of the product, please observe the applicable legal regulations. Information on the disposal of electrical and electronic devices in the European Community The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).
Transport, storage and disposal Mastercycler pro English (EN)
Technical data Mastercycler pro English (EN) 13 13.1 Technical data Power supply Voltage 100 to 130 V 200 to 240 V Frequency 50 Hz to 60 Hz Power consumption 950 W Overvoltage category II (IEC 610 10-1) Degree of contamination: 2 Protection class 1 13.2 Weight/dimensions Dimensions (W x D x H) 26.0 cm × 41.5 cm × 37.0 cm (without control panel) 26.0 cm × 50.0 cm × 44.5 cm (with lid in cleaning position) Depth with the control panel connected: + 14 cm. Weight 18.
Technical data Mastercycler pro English (EN) 13.4 Application parameters Parameter Mastercycler pro Mastercycler pro S Sample capacity 96 x 0.
Ordering Information Mastercycler pro English (EN) 14 14.1 Ordering Information Mastercycler pro Order no. (International) Order no. (North America) 6321 000.019 6321 000.027 950030010 6325 000.013 6325 000.021 950030020 6324 000.010 6324 000.028 6320 000.007 950030030 950030050 14.
Ordering Information Mastercycler pro English (EN) 14.3 Consumables Order no. (International) Order no. (North America) 0030 128.648 951020401 0030 128.575 951020303 0030 133.307 0030 133.358 0030 133.366 0030 133.374 0030133307 0030133358 0030133366 0030133374 0030 129.300 0030129300 0030 129.326 0030129326 0030 129.504 0030129504 0030 129.512 0030129512 0030 129.342 0030129342 0030 128.508 951020702 0030 124.332 951010006 0030 124.359 951010022 0030 124.
Ordering Information Mastercycler pro English (EN) Order no. (International) Order no. (North America) 0030 127.838 0030127838 0030 127.854 0030127854 0030 127.781 0030127781 0030 127.790 0030127790 Description Heat Sealing PCR Film 100 pieces Heat Sealing PCR Foil 100 pieces PCR Film adhesive, 100 pieces PCR Foil adhesive, 100 pieces The twin.tec PCR plates are also available in various frame colors (red, yellow, green, blue).
Index Mastercycler pro English (EN) Index D Date + Time .......................................................... 58 1 1 … 3 StepCyc (program step) ..............................44 A Access rights .........................................................55 Address display .....................................................29 Administrator functions.........................................55 Alarm Activate ............................................................58 Deactivate ...................
Index Mastercycler pro English (EN) I Impulse..................................................................43 Incubate.................................................................60 K Keys .......................................................................30 L Log file Display .............................................................58 Export...............................................................58 Login .....................................................................
Index Mastercycler pro English (EN) R Restart ...................................................................60 Restore ..................................................................62 S safe (header setting)..............................................43 Safety instructions Meaning of symbols...........................................7 Sample tubes .........................................................49 Sample volume ......................................................
Indice Mastercycler pro Italiano (IT) Indice Istruzioni Mastercycler vedi a Tab. Fig. pag. per pro l'uso Italiano Istruzioni (IT) per l'uso 1 Descrizione del prodotto . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 1.1 Illustrazione generale . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 2 Avvertenze di sicurezza generali . . . . . . . . . . . . . . . . . . . .
Indice Mastercycler pro Italiano (IT)
Descrizione del prodotto Mastercycler pro Italiano (IT) 1 Descrizione del prodotto 1.1 Illustrazione generale Abb. 1-1: Mastercycler pro - Vista anteriore e posteriore 1 2 34 5 6 7 8 11 9 10 Fig. 1-1: Mastercycler pro - Vista anteriore e posteriore 1 Maniglia coperchio 7 Interruttore principale Apertura e chiusura nonché blocco e sblocco del 0 = off, I = on. coperchio riscaldato. 8 Morsettiera 2 Coperchio riscaldato vapo.protect Vedi disegno esploso (Fig. 1-2 a pag. 94).
Descrizione del prodotto Mastercycler pro Italiano (IT) Abb. 1-2: Morsettiera Fig. 1-2: Printer RS 232 Control Panel 1 2 3 CAN_out CAN_in Term Address 4 5 6 7 Morsettiera 1 Interfaccia parallela Collegamento per stampante. 2 Interfaccia seriale (RS 232) Collegamento per sistema di validazione temperatura Eppendorf. 3 Collegamento Control Panel 4 CAN out Uscita dati per il collegamento di diversi Mastercycler pro oppure Mastercycler ep.
Avvertenze di sicurezza generali Mastercycler pro Italiano (IT) 2 Avvertenze di sicurezza generali 2.1 Uso conforme Il Mastercycler pro è progettato per l'impiego in laboratori di ricerca e di routine delle bioscienze. Il Mastercycler pro serve per l'incubazione di soluzioni acquose sospensioni o emulsioni in provette di reazione chiuse per le reazioni enzimatiche, in particolare per la reazione della catena polimerasi (PCR).
Avvertenze di sicurezza generali Mastercycler pro Italiano (IT) 2.4 Pericoli in caso di uso conforme Leggere in primo luogo le istruzioni per l'uso e attenersi alle seguenti avvertenze di sicurezza prima di utilizzare il Mastercycler pro. PERICOLO! Pericolo di esplosione! Non azionare l'apparecchio in ambienti in cui vengono utilizzate sostanze potenzialmente esplosive. Non utilizzare per l'apparecchio sostanze esplosive, radioattive o fortemente reattive.
Avvertenze di sicurezza generali Mastercycler pro Italiano (IT) AVVERTENZA! Danni dovuti a tensione di alimentazione non corretta. Collegare l'apparecchio a sorgenti di tensione conformi ai parametri elettrici riportati sulla targhetta del modello. Utilizzare esclusivamente prese con conduttore di protezione. AVVERTENZA! Lesioni da impigliamento o schiacciamento.
Avvertenze di sicurezza generali Mastercycler pro Italiano (IT) ATTENZIONE! Fuoriuscita di materiale campione a causa di provette non adatte. L'utilizzo di provette non adatte può danneggiare le provette stesse e quindi causare la fuoriuscita del materiale dei campioni Utilizzare sempre delle provette con caratteristiche che soddisfino i requisiti minimi riportati nelle istruzioni per l'uso.
Avvertenze di sicurezza generali Mastercycler pro Italiano (IT) ATTENZIONE! Danni ai componenti elettronici dovuti a formazione di condensa. In seguito al trasporto dell'apparecchio da un ambiente freddo a un ambiente più caldo si può formare della condensa all'interno dell'apparecchio stesso. Lasciare che l'apparecchio si riscaldi per 12 ore dopo il montaggio prima di collegarlo all'alimentazione.
Avvertenze di sicurezza generali Mastercycler pro Italiano (IT) 2.5 Spie di avvertimento sull'apparecchio Il presente paragrafo illustra le spie di avvertimento presenti sull' Mastercycler pro. 1 2 1 Attenzione Pericolo di ustioni a causa del blocco termico rovente (triangolo con punta rivolta verso il basso) oppure lato interno del coperchio rovente (triangolo con punta rivolta verso l'alto).
Uso Mastercycler pro Italiano (IT) 3 Uso 3.1 Prime fasi Verificare la corretta installazione Prima di mettere il funzione il Mastercycler pro per la prima volta, accertarsi che: • l'apparecchio sia correttamente collegato. • l'apparecchio non sia danneggiato. • che sia presente una circolazione d'aria priva di impedimenti in corrispondenza delle ferritoie di ventilazione sul lato posteriore dell'apparecchio e del ventilatore dietro ai piedi di sostegno. 3.1.
Uso Mastercycler pro Italiano (IT) 1 2 1. vapo.protect-Tirare in avanti il coperchio riscaldato per la maniglia del coperchio stesso sino a portarlo a fine corsa in modo tale da sbloccare il movimento rotatorio del coperchio. 2. Ribaltare la maniglia del coperchio al di sopra del coperchio riscaldato sino a farla scattare in posizione. È possibile avviare solo un ciclo programma oppure eseguire un'incubazione manuale del coperchio riscaldato e dei relativi campioni. 3.1.1.
Uso Mastercycler pro Italiano (IT) 3.1.1.3 Spia di stato Il coperchio riscaldato del vapo.protect è dotato di una spia di stato 2 (vedi Illustrazione generale a pag. 93). In questo modo è possibile sapere, anche a distanza, in quale stato si trova il Mastercycler pro. Spia di stato Stato operativo si illumina di colore verde Il coperchio riscaldato del Mastercycler pro si trova in folle. lampeggia in colore verde Un ciclo programma è attivo.
Uso Mastercycler pro Italiano (IT) • Navigazione passo passo. Viene evidenziata la posizione del cursore. • Spostamento del cursore all'interno del campo di inserimento alfanumerico. Spostare il cursore sino al campo di inserimento successivo. Next Cancellazione degli elementi: • Simboli che si trovano a destra del cursore. • Simboli evidenziati. • Cartella o programmi evidenziati. Delete • Confermare la scelta. • Aprire l'elenco delle selezioni possibili.
Avvio rapido Mastercycler pro Italiano (IT) 4 Avvio rapido 4.1 Login 1. Aprire l'elenco. Enter 2. Selezionare l'utente. 3. Confermare la scelta. Enter 4. Modificare il PIN nel campo. 1 4 2 abc 5 ghi jkl 7 8 pqrs tuv 0 . Enter 3 def 6 mno 9 wxyz Delete 5. Inserire e confermare il PIN.
Avvio rapido Mastercycler pro Italiano (IT) 4.2 Posizionare la cartella e i programmi 1. Selezionare l'utente o la cartella. 2. Premere NewFolder oppure Premere New. 1 4 ghi 7 2 3 abc def 5 6 jkl 8 pqrs tuv 0 . 3. Inserire nome e commento e confermare. mno 9 wxyz Delete Enter 4.3 Copiare la cartella e i programmi 1. Selezionare la cartella o il programma. 2. Premere il tasto funzione Copy. 3. Confermare la selezione. Enter 4. Evidenziare la cartella di arrivo oppure l'utente.
Avvio rapido Mastercycler pro Italiano (IT) 4.4 Cancellare cartella e programmi 1. Evidenziare cartella e programmi. 2. Premere il tasto Delete. Delete 3. Confermare l'indicazione. Enter 4.5 Modificare il programma 1. Selezionare il programma. 2. Aprire l'editor del programma. Enter 3. Premere il tasto funzione Header. 6. Impostare le funzioni del coperchio e la modalità di controllo. 7. Confermare le scelte.
Avvio rapido Mastercycler pro Italiano (IT) 8. Premere il tasto funzione Insert. 9. Selezionare la fase del programma. 1 4 2 abc 5 ghi jkl 7 8 pqrs tuv 0 . 3 10. Inserire le temperature, le durate degli step e il numero di cicli. 11. Selezionare la fase del programma. 12. Premere il tasto funzione Options. def 6 mno 9 wxyz Delete 13. Impostare gradiente, incremento e rampa. 1 4 2 abc 5 ghi jkl 7 8 pqrs tuv 0 . 3 def 6 mno 9 wxyz Delete 14. Confermare le scelte.
Avvio rapido Mastercycler pro Italiano (IT) 4.6 Avviare e arrestare il programma 1. Evidenziare il programma. Start 2. Inserire le provette campione. 3. Chiudere il coperchio riscaldato. 4. Avviare il programma. 5. Se sono collegati diversi Cycler: evidenziare il Cycler. 6. Confermare la scelta. Enter 7. Tramite la panoramica stato è possibile • arrestare il programma (Stop), • interrompere il programma (Abort), • riavviare il programma (Resume). 4.7 Spegnimento dell'apparecchio 1.
Avvio rapido Mastercycler pro Italiano (IT)
Manutenzione Mastercycler pro Italiano (IT) 5 Manutenzione 5.1 Pulizia PERICOLO! Scosse elettriche. Prima di aprire l'apparecchio, eseguire lavori sui collegamenti elettrici o avviare procedure di manutenzione o pulizia, spegnere l'apparecchio e staccare la spina. AVVERTENZA! Pericolo di combustione a causa del blocco termico e del coperchio riscaldato roventi.
Manutenzione Mastercycler pro Italiano (IT) ATTENZIONE! Danni al coperchio riscaldato determinato dalle provette dei campioni o da altri oggetti a spigoli vivi. Utilizzare sempre delle provette con caratteristiche che soddisfino i requisiti minimi riportati nelle istruzioni per l'uso. Non utilizzare oggetti a spigoli vivi oppure appuntiti in corrispondenza del coperchio riscaldato. Durante la pulizia fare attenzione a non danneggiare il coperchio riscaldato.
Manutenzione Mastercycler pro Italiano (IT) 5.1.2 Pulizia del coperchio riscaldato 5.1.2.1 Posizionare il coperchio riscaldato nella posizione di pulizia 1. Spostare il coperchio riscaldato nella posizione arretrata. 2 1 2. Spingere verso l'alto le linguette a sinistra e a destra al di sotto del bordo inferiore del coperchio riscaldato (1) e allentare la chiusura. 3. Chiudere il coperchio riscaldato (2) sino al punto in cui la staffa di sostegno scatta in posizione.
Manutenzione Mastercycler pro Italiano (IT) Fare in modo che sulle alesature del blocco non si depositino polvere o peli. Anche delle piccole quantità una volta depositatesi possono ostacolare il trasferimento di calore tra blocco e provette dei campioni. 5.1.2.2 Abbandonare la posizione di pulizia 1. Tenere il coperchio in corrispondenza del margine anteriore con la mano destra e tirare il supporto di montaggio 1 in avanti con la mano sinistra, per allentare il fermo. 2 1 2.
Manutenzione Mastercycler pro Italiano (IT) Liquidi ausiliari necessari • Alcol (etanolo, isopropanolo) oppure altri disinfettanti contenenti alcol • Detergente da casa non aggressivo a base di sapone Procedere nel seguente modo: 1. Selezionare un metodo di disinfezione che ottemperi alle disposizioni di legge e alle linee guida vigenti nel campo di applicazione. 2. Spegnere l'apparecchio e staccarlo dalla presa di corrente. 3.
Manutenzione Mastercycler pro Italiano (IT)
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