R/170 nual S CO Incubators gEN) manual 2 New Brunswick™ Galaxy® 170 R/170 S CO2 Incubators Operating manual
Copyright Copyright © 2014 Eppendorf AG, Germany. No part of this publication may be reproduced without the prior permission of the copyright owner. The company reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement. Trademarks Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.
Table of contents Galaxy® 170 R/170 S CO2 Incubators English (EN) Table of contents 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Table of contents Galaxy® 170 R/170 S CO2 Incubators English (EN) 6.2 6.3 6.4 6.5 6.6 6.7 6.8 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 6.2.1 Temperature and CO2 level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 6.2.2 User access code. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 6.2.
Table of contents Galaxy® 170 R/170 S CO2 Incubators English (EN) 8.2 8.3 8.4 8.5 9 8.1.6 Routine checks of the O2 control option (170 R only). . . . . . . . . . . . . . . . . . . . . . . . . . 52 Service. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 8.2.1 Fuse replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Cleaning . . . . . . . . . . . .
Table of contents Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.4.9 Troubleshooting the O2 sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 12.4.10 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79 12.5 O2 control (0.1 - 19 %) (Galaxy R only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 12.5.1 Setting up the N2 tank. . . . . . . .
Operating instructions Galaxy® 170 R/170 S CO2 Incubators English (EN) 1 1.1 Operating instructions Using this manual Carefully read this operating manual before using the device for the first time. Also observe the operating manual enclosed with the accessories. The operating manual should be considered as part of the product and stored in a location that is easily accessible. When passing the device on to third parties, be sure to include this operating manual.
Operating instructions Galaxy® 170 R/170 S CO2 Incubators English (EN) 1.3 Symbols used Example Meaning You are requested to perform an action. 1. 2. Perform these actions in the sequence described. • List. References useful information.
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN) 2 2.1 Product description Main illustration 2.1.1 Galaxy 170 R/170 S CO2 Incubators Abb. 2-1: Front and rear view of the 170 R/170 S CO2 Incubators 1 2 4 3 8 Fig.
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 2-2: Left-hand side control box 2 1 3 Fig.
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 2-3: Right-hand side control box 1 2 7 6 3 5 4 Fig.
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN) 2.
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN) 2.2.2 Packing list verification Unpack your order, saving the packing materials for possible future use. Save the operating manual for instruction and reference. Verify against your Eppendorf packing list that you have received the correct materials, and that nothing is missing.
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN) 2.3.5 Seamless chamber The 170-liter chamber is seamless, to provide a sanitary and easy-to-clean environment, and all internal components are manufactured from polished stainless steel. The shelves (which are non-tip), shelf racks and humidity tray are easily removed without tools for thorough cleaning and are capable of being sterilized.
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN) 2.5 Optional equipment The equipment options are factory-installed and model-dependent. A precise description of the equipment options can be found in this manual (see Equipment options on p. 65).
Product description Galaxy® 170 R/170 S CO2 Incubators English (EN)
Safety Galaxy® 170 R/170 S CO2 Incubators English (EN) 3 3.1 Safety Intended use Eppendorf line of CO2 Incubators are microprocessor-controlled instruments designed for cell culture. The direct-heated, fanless chambers are designed to provide high humidity levels, minimal vibration and precisely-regulated atmosphere of temperature and gas(ses) required for cell growth in T-flasks, microplates, and other cultureware. They are intended for indoor laboratory use, only.
Safety Galaxy® 170 R/170 S CO2 Incubators English (EN) 3.4 Information on product liability In the following cases, the designated protection of the device may be compromised. The liability for the function of the device passes to the operator if: • The device is not used in accordance with this operating manual. • The device is used outside of the range of application described in the succeding chapters. • The device is used with accessories or consumables that were not approved by Eppendorf.
Safety Galaxy® 170 R/170 S CO2 Incubators English (EN) NOTICE! Risk of material damage CO2 gas pressure must not exceed 5 PSI (0.35 bar). NOTICE! Risk of material damage Working with electrical power inside a humid environment (where the incubator is humidified) can cause damage.
Safety Galaxy® 170 R/170 S CO2 Incubators English (EN)
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 4 4.1 Installation Utilities requirements The following utilities requirements are needed for operation: Utility Requirement Electricity 120 V, 50/60 Hz earthed/grounded mains/electrical supply with minimum capacity of 10 amps 230 V, 50/60 Hz earthed/grounded mains/electrical supply with minimum capacity of 8 amps CO2 gas Cylinder with 100 % CO2 vapor withdrawal, together with a two-stage regulator for pressure control to 18.85 PSI (1.
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 4.3 Unpacking the incubator CAUTION! Risk of personal injury At least four people are required to safely lift the incubator. NOTICE! Risk of material damage Never try to lift the incubator by its door; this would cause permanent damage to the incubator. 1. Install the four lifting handles into the tapped holes on both sides of the incubator. Abb. 4-1: Installing lifting handles 1 2 Fig.
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 4-2: Inserting shelf racks 1 Fig. 4-2: 1 2 Inserting shelf racks 1 Shelf rack feet 2 Shelf rack spacer Only shelf racks for four shelves are shown in this manual. Shelf racks for eight shelves are also available. 4. Install the tie rod at the back of the shelves to hold both sides together.
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 4-3: Installing tie bar to shelf racks 1 2 Fig. 4-3: Installing tie bar to shelf racks 1 Tie rod 2 Tie rod anti-tip groove 5. Install the shelves, top to bottom. Ensure that each shelf’s anti-tip groove is properly inserted (faced upward and to the rear of the incubator) onto each of the shelf rack guides. Abb. 4-4: Inserting shelves Fig.
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 6. Level the incubator by adjusting the feet. Place a small level on the second shelf of the incubator. Adjust the leveling feet until the incubator is level and stable. Lock the leveling feet in place by tightening the locking nuts on each foot. 7. Slide the humidity tray onto the lowest shelf rack support. Abb. 4-5: Installing the humidity tray Fig. 4-5: Installing the humidity tray Abb. 4-6: Humidity tray installed Fig.
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 4.5 Making connections WARNING! Risk of personal injury Elevated levels of CO2 may be found in and around the operating area of the CO2 incubator. Wear personal protective equipment (PPE). WARNING! Danger due to incorrect power supply Only connect the device to voltage sources that meet the requirements on the name plate. Only use sockets with a protective earth (PE) conductor and suitable power cable. 1.
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 3. Attach supplied 6 mm bore PVC hose with large white filter to gas inlet connector. Abb. 4-7: Making connections 1 2 7 6 3 5 4 Fig. 4-7: Making connections 1 On/Off switch 2 Mains/power cord receptacle 3 Label 4 Fuse holder 5 N2 inlet (170 R optional only, option not available on 170 S) 6 CO2 inlet 7 O2 inlet (170 R optional only, option not available on 170 S) 4.
Installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Attaching in-line pressure regulator, (not supplied), is a recommended option. Abb. 4-8: In-line pressure regulator Fig. 4-8: In-line pressure regulator • The in-line pressure regulator controls Secondary Gas Pressure. A default pressure setting of 5 PSI (0.35 bar) is recommended. • A large size cylinder of “CO2 - Vapour Withdrawal” is required to supply the incubator. This cylinder controls Primary Gas Pressure.
Operation Galaxy® 170 R/170 S CO2 Incubators English (EN) 5 5.1 Operation Preparing for operation 1. Remove the black protective cover from the CO2 sensor, taking care not to remove the white porous cover; store the black cover on the sensor cover holder on the side of the incubator. The sensor cap should be placed back on the sensor when the incubator is to be cleaned. Abb. 5-1: CO2 Incubator rear view 1 2 3 6 5 4 Fig.
Operation Galaxy® 170 R/170 S CO2 Incubators English (EN) • The incubator’s CO2 valve is disabled until the incubator reaches the temperature setpoint. After the temperature setpoint is reached, the CO2 valve is activated, allowing the incubator to reach the CO2 setpoint. • If power is interrupted to the incubator long enough for the temperature to drop below setpoint, the CO2 valve will be deactivated until temperature setpoint is again achieved.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 6 6.1 Operating controls and function for Galaxy 170 R Incubator 170 R control panel The control panel consists of an LCD display, five function keys and four direction keys. Abb. 6-1: Galaxy 170 R control panel (normal operation) 92 2 1 3 Fig.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 6.2 Programming 6.2.1 Temperature and CO2 level Perform the following steps to set the desired operating temperature and CO2 level. For more information on this feature (see Infrared sensor on p. 13). 1. Press the PROG function key. 2. In the PROG screen that appears, press the desired function key, TEMP or CO2, then use the direction keys to adjust the value.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) If the access code has been misplaced, you will be unable to make changes to your incubator’s settings. Contact customer service or your service representative for instructions on how to regain access to your incubator. 6.3 Referencing the CO2 sensor with Auto-Zero Prior to using the incubator, you should manually perform a CO2 Auto-Zero (see PROGRAMMABLE CO2 AUTOZERO on p. 34): 1.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 6.4.1 SET DATE AND TIME The date and time is factory set and will only require adjustment if you are in a different time zone, or when you change your clocks to Daylight Saving Time and back again to Standard time. You may also select the style of display for the date. 6.4.2 AUDIBLE ALARM VOLUME ADJUST The audible alarm volume can be adjusted to your own preferences. 6.4.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 4. The result of the Auto-Zero (listed as A/Z on some screens) is sent to the DATALOGGER ALARM EVENTS screen so that a record of the results will be kept. The frequency of Auto-Zeroing can be set in steps between once a day and once every 28 days. The default setting is once every 28 days. If not required, it can be disabled (see DISABLE on p. 35). The default time setting is 7:00 am.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) disinfected prior to resumption of activity. For a full explanation of this feature, (see High temperature disinfection option with oxygen control on p. 71). 6.5 DATALOGGER screen The DATALOGGER screen displays the following information: Abb. 6-4: DATALOGGER screen Fig. 6-4: 6.5.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 6-5: ALARM EVENTS screen Fig. 6-5: 6.5.2 ALARM EVENTS screen TEMPERATURE GRAPH + DOOR OPEN BAR CHART When you select this from the DATALOGGER screen, the Door Open bar chart is shown at the top of the screen to associate it with a temperature disturbance (see Fig. 6-6 on p. 38). A temperature reading is recorded every 18 seconds while the temperature is outside the specification of ±0.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 6-6: TEMPERATURE GRAPH + DOOR OPEN BAR CHART screen Fig. 6-6: TEMPERATURE GRAPH + DOOR OPEN BAR CHART screen Abb. 6-7: Dotted vertical line showing compressed/decompressed data. Fig. 6-7: Dotted vertical line showing compressed/decompressed data. Compressing data allows memory space to be maximized.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 6.5.5 DIAGNOSTIC DOOR GRAPH This graph records the door’s inner surface temperature over time to assist troubleshooting. 6.5.6 DIAGNOSTIC DOOR ELEMENT GRAPH This graph records door element temperature over time to assist troubleshooting. 6.5.7 RESTART GRAPHIC RECORD This feature removes the current graph and begins a new one. The data cannot be recovered once it is deleted. 6.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 2. Select YES for both TEMP and CO2. 3. When YES is selected for this function, the DELAY IN ARMING AFTER DOOR OPEN is ignored. A DOOR OPEN ALARM: can be adjusted, choosing from seven preset durations (45 seconds in (Fig. 6-8 on p. 39)) to warn of an improperly closed door.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 6-10: CHAMBER ALARM to check CO2 supply Fig. 6-10: CHAMBER ALARM to check CO2 supply Instructions to remedy the alarm are provided in the ALARM screen. 6.7 DIAGNOSTICS menu screen The diagnostics screen contains technical information regarding the status of many of the system components found on the incubator.
Operating controls and function for Galaxy 170 R Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 6.8 HELP MENU screen The HELP MENU screen provides user-selectable categories of abbreviated information found in the user manual. All the major systems are covered in the help menu, including help on installing the incubator. If the user manual is misplaced, information about the CO2 incubator and its functions can always be found on-screen. Abb. 6-12: HELP MENU Screen Fig.
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 7 7.1 Operating controls and function for Galaxy 170 S Incubator 170 S control panel The control panel consists of two individual three-digit LED displays, and four function keys: Abb. 7-1: Galaxy 170 S control panel (normal operation) 1 2 3 4 5 6 Fig.
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 7.2 Setting temperature and CO2 Perform the following steps to program the temperature and CO2 setpoints. Temperature and CO2 may be set within the ranges shown in the following table: Tab. 7-1: Setting temperature and CO2 Parameter Available Setpoint Range Temperature 10 °C to 50 °C (must be at least 4 °C above ambient) CO2 0.2 % to 20 % To set the Temperature: 1.
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 6. Press the Enter key to accept the setting. 7. Press the Programming ( * ) key twice to exit the menu. 7.3.2 Setting the CO2 high and low alarms 1. Press the Enter and keys simultaneously to enter the alarm menu. The display will show: °C.AL. key until the display shows: CO2AL. 2. Press the 3. Press the Enter key to display HI.5.5. The factory setting is the setpoint value (5.0 %) + 0.5 %. 4.
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) As you scroll through the available choices, you will see OFF, 10 sec, 30 sec, 60 sec, 600 sec, 1 HR, then ON). 4. Press the Enter key to select the desired value. 5. Press the Programming ( * ) key twice to exit the menu. 7.3.
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 7.4.1 Temperature sensor system alarms There are six temperature sensors: two in the chamber, two in the door and two in the base. If any of these sensors should fail, the following message will appear: ºC F.AIL. Because the incubator can no longer control temperature properly without the failed sensor, the heating will switch off and the incubator will cool down to room temperature.
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 2. Press the Enter key to begin the cycle. The Auto Zero System automatically re-references the CO2 Sensor to atmospheric CO2 in the following way: • A small pump switches on for 2 minutes pumping filtered atmosphere at 0.3 liters/minute into the measuring chamber of the sensor. This displaces the chamber atmosphere completely from the sensor.
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN) 7.5.3 High temperature disinfection operation For high temperature disinfection information (see Using the high temperature disinfection (Galaxy S) on p. 70).
Operating controls and function for Galaxy 170 S Incubator Galaxy® 170 R/170 S CO2 Incubators English (EN)
Maintenance Galaxy® 170 R/170 S CO2 Incubators English (EN) 8 8.1 Maintenance Routine maintenance 8.1.1 General WARNING! Risk of injury to personnel or damage to equipment! Two stacked incubators should not be moved for maintenance or cleaning. To ensure that chamber conditions remain stable, minimize the length of time that the door is open. When you open the door, wipe off any condensation that may have formed on the inner seal to avoid condensation build-up.
Maintenance Galaxy® 170 R/170 S CO2 Incubators English (EN) If required, you can take a sample of the gas inside the chamber using the CO2 sample port, and check it using a CO2 gas analyzer (see Accessories on p. 63)(see CO2 Sampling with analyzer on p. 52). Displayed chamber CO2 level will drop during sampling, but it will recover once the sampling is complete. This is merely a sensor characteristic; the CO2 level in the chamber is actually not affected.
Maintenance Galaxy® 170 R/170 S CO2 Incubators English (EN) 8.2 Service 8.2.1 Fuse replacement DANGER! Electric shock Switch the device off and pull the power plug out of the socket before beginning work. To replace a fuse, you will need a flat-bladed (5 mm maximum) screwdriver. 1. Using the flat-bladed screwdriver, rotate the fuse holder (see Fig. 4-7 on p. 27) counter-clockwise until the fuse holder springs out. 2. Remove the spent fuse. 3. Install a new fuse of the same type. 4.
Maintenance Galaxy® 170 R/170 S CO2 Incubators English (EN) NOTICE! Risk of material damage Never use any of the following substances to clean the stainless steel, or damage will result: Sodium Azide, Aqua Regia, Iodine, Ferric Chloride or Sulphuric Acid. Use the decontamination method recommended by the manufacturer. If you would like to use a different method, contact Eppendorf to prevent the device from becoming damaged.
Maintenance Galaxy® 170 R/170 S CO2 Incubators English (EN) 10. Refill the humidity tray (see Using the humidity tray on p. 30). When you reinstall it, ensure that the humidity tray is pushed fully back. 11. Leave the incubator on for at least two hours (preferably overnight) to allow conditions to stabilize. 12. When the incubator has stabilized, carry out an Auto-Zero and reprogram the desired CO2 level.
Maintenance Galaxy® 170 R/170 S CO2 Incubators English (EN)
Transport, storage and disposal Galaxy® 170 R/170 S CO2 Incubators English (EN) 9 9.1 Transport, storage and disposal Transport NOTICE! Risk of material damage Never try to lift the incubator by its door; this would cause permanent damage to the incubator. 9.2 Storage Store incubator in ambient conditions of 10 °C - 50 °C. 9.3 Disposal In case the product is to be disposed of, the relevant legal regulations are to be observed.
Transport, storage and disposal Galaxy® 170 R/170 S CO2 Incubators English (EN)
Technical data Galaxy® 170 R/170 S CO2 Incubators English (EN) 10 10.1 Technical data Weight/dimensions 10.1.1 Equipment dimensions Width 68.5 cm (27 in) Height 84.3 cm (33.2 in) Depth 67.7 cm (26.7 in) Weight 90 kg (198.4 lb) 10.1.2 Internal dimensions Width 53.3 cm (21.0 in) Height 69.3 cm (27.3 in) Depth 44.4 cm (17.5 in) Volume 174 liters 10.1.3 Transporting dimensions Width 83.0 cm (33 in), pallet included Height 110.0 cm (43 in), pallet included Depth 83.
Technical data Galaxy® 170 R/170 S CO2 Incubators English (EN) 10.2 Power supply 10.2.1 Mains/electrical supply Mains/power connection 120 V, 50 Hz 120 V, 60 Hz 230 V, 50 Hz 230 V, 60 Hz Power consumption for 120 V 1300 W Power consumption for 230 V 1600 W Energy to maintain 37 °C < 0.1 kWh 10.3 Fuses fuse for 120 V 10 A fuse for 230 V 8A 10.4 Ambient conditions Ambient conditions 10.4.1 15 °C - 28 °C Temperature management • Digital programming via microprocessor control in 0.
Technical data Galaxy® 170 R/170 S CO2 Incubators English (EN) 10.4.2 CO2 control Solid-state infrared CO2 sensor operating independent of humidity. Programmable, fully automatic zeroing function. Range 0.2 - 20 % Control ± 0.1 % Stability ± 0.2 % at 5 % CO2 Uniformity ± 0.1 % Gas connections 6 mm tubing Required gas pressure 5 PSI (0.35 bar) 10.4.3 Relative humidity Removable stainless steel humidity tray. Reservoir capacity 2.5 liters Humidity control Normal: 95 % at 37 °C 10.4.
Technical data Galaxy® 170 R/170 S CO2 Incubators English (EN)
Ordering information Galaxy® 170 R/170 S CO2 Incubators English (EN) 11 11.1 Ordering information Accessories Contact Eppendorf sales for ordering information.
Ordering information Galaxy® 170 R/170 S CO2 Incubators English (EN) 11.2 Available options Some option combinations are not possible, others may incur extra cost. Contact Eppendorf sales for ordering information. Options Cooling System Galaxy 170 R BMS Relay Galaxy 170 S BMS Relay O2 Control, 0.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12 12.1 Equipment options Cooling system The cooling system option allows the incubator to be used in one of three possible modes. The cooling system option consists of a heat exchanger assembly, fitted to the rear wall of the chamber, with a circulation fan. Inside the incubator chamber, the heat exchanger cooling fins and fan are enclosed by a removable cover, with ventilation slots to facilitate airflow to the fan and to the shelves.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.1.1 Troubleshooting the cooling system The thermo-electric module which governs the cooling system is self-contained and does not require any specific maintenance during the life of the incubator. If, however, the cooling system fails to perform according to expectation, perform the following steps: 1. Check that the incubator has been set to the desired cooling mode.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 12-1: BMS relay contact alarm socket Fig. 12-1: BMS relay contact alarm socket Pin Designation 1 24 V DC unregulated (170 R Models) 12 V DC unregulated (170 S Models) To power external equipment such as a remote buzzer or light (100 mA maximum current available).* 2 0V 3 5 V DC Via 10 K Ω pull-up resistor, for a logic signal to directly control an auxiliary control system.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.3 High temperature disinfection The High Temperature Disinfection option is designed to heat the internal chamber to 120 °C, maintain that temperature for 4 hours, and then allow the chamber to cool down to 37 °C or to the programmed temperature (if different from 37 °C) when normal control takes over.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) was completed successfully, DISINFECTION COMPLETED OK is shown. was cancelled by the user, DISINFECTION WAS ABORTED is shown. failed for any reason, DISINFECTION FAILED [CODE: XX] is shown. The following tables lists the disinfection failure codes and descriptions, (see Tab. on p. 69) and (see Tab. on p. 69). If this happens, note the failure code and contact your service representative for advice.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.3.2 Using the high temperature disinfection (Galaxy S) Prerequisites • The incubator should be cleaned, disinfected, and dried thoroughly before starting the cycle, (see Disinfection/Decontamination on p. 53). • The black protective cover must be removed (the white porous cover can remain in place). • The shelves, shelf racks, humidity tray and silicone rubber feet and sleeves should all be in place during the cycle.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) – DEC.ON FAI.LED • If the message says that disinfection failed, it will be followed by any combination of the following messages: – FAL.L DUR.ING HEA.T (this indicates that the temperature dropped more than 2 °C over a 60-second period during the heating phase) – FAL.L DUR.ING DEC.ON (this indicates that the temperature fell below 118.0 °C during the disinfection phase) – RIS.E DUR.ING COO.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) Detailed removal and installation instructions are provided (see Removing and replacing O2 sensor on p. 76). 12.4 O2 control (1 - 19 %) (Galaxy R only) This oxygen control option is designed to cover the 1 - 19 % range by adding nitrogen to bring the level below ambient. If you have the 0.1 - 19 % oxygen control option, (see O2 control (0.1 - 19 %) (Galaxy R only) on p.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) If the programmed O2 level is close to the ambient oxygen, it may be necessary to reduce the cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed value. Both inner and outer doors must be open. 4. After the incubator has been humidified and left overnight to stabilize, select the USER menu; then, using the or direction key, select OXYGEN SENSOR-REF TO ATMOSPHERE (see Fig. 12-2 on p.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) At low oxygen levels, the CO2 and O2 levels may not have fully recovered within the Alarm limits after the 15-minute “Delay in arming after door opening.” This time period can be increased (in the ALARM screen) to suit individual circumstances. 8. By controlling the Duty Cycle of the N2 valve, Oxygen Control can be tailored to achieve programmed Oxygen and Carbon Dioxide levels at approximately the same time. 12.4.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.4.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) OXYGEN CONTROL HAS BEEN DISABLED AS A RESULT BUT THE INCUBATOR IS OTHERWISE FULLY OPERATIONAL. PRESS PREV TO VIEW PREVIOUS SCREEN. PRESS EXIT TO EXIT. When you press the EXIT function key, you will return to the USER screen and normal operation. 12.4.7 Removing and replacing O2 sensor Prerequisites • Oxygen sensor removal tool (see Fig. 12-5 on p.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 12-5: Oxygen sensor removal tool Fig. 12-5: Oxygen sensor removal tool 4. Using the sensor removal tool, install the new oxygen sensor by turning it clockwise until it is finger-tight. Do not use excessive force or any metal tool. 5. Reconnect the sensor by plugging the white connector body back in. 6.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 12-6: Hydrophobic filter and holder assembly (exploded view) 1 2 3 5 4 Fig. 12-6: Hydrophobic filter and holder assembly (exploded view) 1 Oxygen sensor holder 4 Hydrophobic filter cap 2 Hydrophobic filter disc 5 Oxygen sensor 3 Hydrophobic filter holder 5. Make sure the O-rings (between the cap & filter holder and between the filter holder & oxygen sensor holder) are undamaged and securely in place. 6.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 4. Reopen the door for 15 seconds to release any build-up of humidity. 5. Repeat steps 3 and 4 every 30 minutes while monitoring the DATALOGGER O2 Graph. The oxygen level should recover after a few hours. 6. Leave the incubator for a few more hours to be ensure that the sensor membrane has thoroughly dried out. 7. Replace the hydrophobic filter disc (see Replacing the filter disc on p. 77). 8. Re-humidify the incubator. 9.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.5 O2 control (0.1 - 19 %) (Galaxy R only) This oxygen control option uses the controlled addition of nitrogen to reduce the oxygen level below ambient. If you have the 1 - 19 % oxygen control option, (see O2 control (1 - 19 %) (Galaxy R only) on p. 72); or if you have the 1 - 95 % oxygen control option, (see O2 control (1 - 95 %) (Galaxy R only) on p. 83). The O2 sensor must be calibrated when used to achieve O2 control below 1 %.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 1. Remove the black plastic protective cover (making sure that the hydrophobic filter cap is not removed with it) from the port inside the chamber. Retain the cover for use when you clean the chamber. 2. Open the nitrogen gas supply from the tank and set the N2 tank’s outlet pressure gauge to 1.5 bar. 3. Set the nitrogen regulator to 14.5 PSI (1 bar).
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.5.6 Programming desired O2 level Program the required oxygen level in the PROG screen, following the onscreen instructions. If you are running an O2 level programmed between 0.1 - 0.9 %, you should know that the control system is set to operate in the following way to minimize N2 consumption after the glass door has been opened: • The N2 valve is switched on continuously until the O2 level is within 0.1 % of setpoint.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.6 O2 control (1 - 95 %) (Galaxy R only) This oxygen control option uses the controlled addition of oxygen to increase the level above ambient and the controlled addition of nitrogen to reduce the oxygen level below ambient. If you have the 1 - 19 % oxygen control option, (see O2 control (1 - 19 %) (Galaxy R only) on p. 72); or for the 0.1 - 19 % oxygen control option, (see O2 control (0.1 - 19 %) (Galaxy R only) on p. 80).
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 4. Securely attach the two-stage pressure regulator to the nitrogen tank’s outlet and to the inline pressure regulator’s inlet with tubing, securing both ends with clips. 5. Connect the inline pressure regulator’s outlet to the incubator’s N2 inlet on the right side of the control box at the rear of the incubator (see Fig. 2-3 on p. 11); secure both ends with clips. 6. Proceed as indicated in (see Setting up O2 control on p. 84).
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) To disable oxygen control, follow the pattern used to enable it, but this time select USER, MANUAL DISABLE, ENTER, OFF and then ENTER again. 12.6.4 Important notes for O2 levels > 80 % If you are running the incubator with a dry chamber and no CO2, the recommended maximum O2 level is 95 %.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) To reference the oxygen sensor to atmospheric oxygen levels, (see Referencing to atmosphere on p. 74). 12.6.7 Programming desired O2 level Program the required oxygen level in the PROG screen, following the onscreen instructions. 12.6.8 Other precautions Under normal humidity conditions (95 - 99 % RH), the sensor is unaffected.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) fuse which is rated at 4 Amps. There is a dedicated switch for this socket, illuminated green when it is on; the switch is located on the left front of the incubator. Abb. 12-7: IP66 Enclosure 1 4 3 2 Fig.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) NOTICE! Risk of material damage Working with electrical power inside a humid environment (where the incubator is humidified) can cause damage. The following precautions should be observed: The instrument or equipment, and its external connections, to be used inside the chamber should be specified as suitable for use in a humid environment, and at 37 °C (see also “Using Powered Equipment within the Chamber”).
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) NOTICE! Risk of material damage The Electrical Socket and the matching plug are both sealed to IP66 and are capable of being heated to 120 °C during the High Temperature Disinfection cycle, but the electrical cable is NOT rated for use at 120 °C. Therefore, the equipment or device and the connecting cable must be removed from the chamber, and the Sealing Cap replaced on the socket prior to starting a High Temperature Disinfection cycle.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) NOTICE! Risk of damage to equipment The bottom surface of the incubator is heated to over 150 °C during the decontamination cycle which may affect the sensor or the cable if left in contact with the bottom of the incubator. Never leave the humidity warning sensor on the bottom surface of the incubator during a high temperature decontamination cycle.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN) 12.10 Copper inner chamber Copper naturally changes color. This incubator had a bright finish when it was manufactured. The dull finish that you see is a result of oxidation of the copper surface: it is the properties of this oxidation that create the important anti-microbial surface.
Equipment options Galaxy® 170 R/170 S CO2 Incubators English (EN)
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 13 13.1 Stacking stand installation Intended use The stacking stand is designed for two Galaxy 170 incubators to be stacked on top of each other. The stand can also be used to stack two Galaxy Plus series (not available in North America) incubators or any combination of a Galaxy Plus model with a Galaxy 170. For optimum stability, if a 170 incubator is being stacked with another Galaxy model, the 170 should be on the bottom. 13.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Kit for Stacking a pre-2000 incubator onto a Galaxy 170 13.3.2 Stacking frame kit contents All of the parts listed for both kits below are included in the Lower and Upper Stacking Frame Kit (P0628-6270). Check that you have received all of the parts appropriate to the kit you ordered: Upper Stacking Frame contents: See the Table below.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) CAUTION! Personnel injury and equipment damage! Four people are required to safely lift a Galaxy 170 incubator. When lifting, be sure to use the lifting handles provided with the incubator. NEVER lift the incubator by the door as this may cause damage. 1. At least two persons are needed to very carefully tip the first incubator forward on its front feet, until the rear castors are accessible. Hold the incubator steady.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 13-2: Feet Installation Fig. 13-2: Feet Installation 4. Adjust the height of the feet to ensure that the incubator is level. The feet on the bottom incubator should ideally be set at a height of 32mm, and the feet on the top incubator at 32mm. 5. Repeat steps 1-4 for the second incubator. 6. Using the wrench provided, fit the adjustable castors to the front underside of the lower stacking frame (see Figure below). Abb.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 7. Using the appropriate Allen wrench in the set provided, install the fixed castors to the rear underside of the lower stacking frame using the M8 button head bolts (see Figure below). Abb. 13-4: Castor Bolts Fig. 13-4: Castor Bolts 8. Turn the lower stacking frame over, ensuring that it is on a flat surface.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 9. With four people, each using one of the lifting handles, lift the bottom incubator onto the stand, placing the incubator feet into the stand’s guiderails (see Figure below). Abb. 13-5: Lifting Handles And Guides For Incubator Feet Fig. 13-5: Lifting Handles And Guides For Incubator Feet 1 Incubator Lifting Handles 2 Guides For Incubator Feet 10.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 13-6: Leveling Foot Adjustment Fig. 13-6: Leveling Foot Adjustment 1 Turn red adjuster in this direction to move foot 3 Rubber Leveling Foot up. 2 Turn red adjuster in this direction to move foot down. 11.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 13-7: Upper Stacking Frame Installation Fig. 13-7: Upper Stacking Frame Installation 12. With four people, each using one of the lifting handles, lift the top incubator onto the upper stacking frame, making sure to place a pad under each foot. Fit the incubator feet into the dimples on the upper stacking frame (see Figure below). Abb. 13-8: Incubator Into The Upper Stacking Frame Fig.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 13. Secure the top incubator by fitting the foot-clamping brackets to its feet. As shown below, with the door open for easy access, install both front brackets using the Allen key set and the M6 socket head bolts provided. Abb. 13-9: Front Bracket Installation Fig. 13-9: Front Bracket Installation Abb. 13-10: Allen Wrench And Bracket Fig. 13-10: Allen Wrench And Bracket 14.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) 13.5 Instructions for stacking a different model CO2 incubator onto a Galaxy 170 WARNING! Personnel injury and equipment damage! Great care should be taken when moving the stacked incubators. Hazards, including uneven surfaces and obstructions, may cause the stacked assembly to topple, potentially injuring personnel.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN) Abb. 13-12: Rotated Channel Sections Fig. 13-12: Rotated Channel Sections 3. The top incubator can now be lifted onto the plate, locating the incubator’s feet within the channel sections.
Stacking stand installation Galaxy® 170 R/170 S CO2 Incubators English (EN)
Declaration of conformity Galaxy® 170 R/170 S CO2 Incubators English (EN) 14 14.
Declaration of conformity Galaxy® 170 R/170 S CO2 Incubators English (EN) 14.
Index Galaxy® 170 R/170 S CO2 Incubators English (EN) Index E Enabling or disabling O2 control.......................... 81 A access code, programming ...................................32 F Alarm arming delay ...............................................46 Fuse replacement.................................................. 53 Alarm duration ......................................................45 Alarm events..........................................................36 Ambient conditions ...........
Index Galaxy® 170 R/170 S CO2 Incubators English (EN) R U Referencing to atmosphere ...................................74 Unpacking boxes .................................................. 13 Removing access code ..........................................32 User requirement.................................................. 17 Removing and replacing O2 sensor ......................76 User settings ......................................................... 33 Replace sensor now...................
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