hmanual ual MultiCon EN) epBlue™ with MultiCon Software manual Software-Version 40.
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Trademarks Eppendorf®, the Eppendorf logo, epMotion®, and epT.I.P.S.® are registered trademarks of Eppendorf AG. Excel® and Windows® are registered trademarks of Microsoft Corporation in the United States and other countries. Registered trademarks are not marked in all cases with ® or ™ in this manual. The software of the device (firmware) contains open source software.
Table of contents epBlue™ with MultiCon English (EN) Table of contents 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2.
Table of contents epBlue™ with MultiCon English (EN) 6 epBlue Application Editor - Creating and editing applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 6.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 6.2 Creating an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 6.2.1 Menu bar . . . . . . . . . . .
Table of contents epBlue™ with MultiCon English (EN) 8 epBlue Labware Editor - Creating and managing labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 8.1 Overview of labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 8.2 Managing labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92 8.2.1 Menu bar . . . . . . . . . . . . . . . . .
Table of contents epBlue™ with MultiCon English (EN) 10 epBlue ID - Working with ID tracking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 10.1 ID lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 10.1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 10.1.
Table of contents epBlue™ with MultiCon English (EN) 13 epBlue Settings - Saving data and creating print templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 13.1 Data backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 13.1.1 Securing the database. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 13.1.2 Creating an automatic backup . . . . . . . . . .
Table of contents epBlue™ with MultiCon English (EN)
Operating instructions epBlue™ with MultiCon English (EN) 1 1.1 Operating instructions Using this manual Your epMotion operating manual consists of hardware instructions and software instructions. Short instructions are available for optional software enhancements. The operating manual is part of the product. The current version of the operating manual can be found on our webpage: www.eppendorf.com. Read the operating manual in full before using the device.
Operating instructions epBlue™ with MultiCon English (EN) 1.2.2 Danger levels DANGER Will lead to severe injuries or death. WARNING Can lead to severe injuries or death. CAUTION May lead to light to moderate injuries. NOTICE May lead to material damage. 1.3 Symbols used Depiction Meaning 1. 2.
Operating instructions epBlue™ with MultiCon English (EN) 1.4 Abbreviations used BCR Bar code reader CSV Comma Separated Value DNA Deoxyribonucleic acid DWP Deepwell plate epT.I.P.S.
Operating instructions epBlue™ with MultiCon English (EN) 1.5 Glossary A Administrator User who has the rights to administrate epBlue. Application Program for a specific application. An application includes the procedure and the equipping of the worktable. B Bar code reader Device for scanning a barcode. Blow-out Movement of the piston. The liquid in the pipette tip will be dispensed after the pipetting step. C CleanCap Optional epMotion equipment.
Operating instructions epBlue™ with MultiCon English (EN) epBlue Software for creating and administering applications and labware. The epMotion is controlled using the software. The software includes a user administration system. Modular extension of epBlue is possible. With the epBlue ID module you can track samples during the application. The labware is labeled with IDs for this purpose. The sample tracking is documented automatically. epT.I.P.S. Motion epMotion pipette tips. Only epT.I.P.S.
Operating instructions epBlue™ with MultiCon English (EN) L Lab administrator User who has the rights to administrate applications and labware. Labware Racks, plates, tips, etc. which are placed on the worktable. Location Area where labware is placed on the worktable. M MagSep System consisting of the magnetic finger module, thermomixer and PrepRack and reagents for nucleic acid preparation with the epMotion. MultiCon epMotion operator panel.
Operating instructions epBlue™ with MultiCon English (EN) S SafeRack Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination of adjacent tips. Use the SafeRacks if you would like to use tips several times. Source labware Rack or plate with source positions. Source location Position in the labware that liquid is aspirated from exclusively during the application. T Thermal module Thermal module for labware that is integrated in the worktable.
Operating instructions epBlue™ with MultiCon English (EN)
Product description epBlue™ with MultiCon English (EN) 2 2.1 Product description Features The epBlue software is used to control the epMotion. epBlue consists of several components. Which modules are available depends on the features of your epMotion and the specification of the software. 2.1.1 epBlue Module Application Editor Description • Create applications. • Manage applications. Operating manual (see epBlue Application Editor - Creating and editing applications on p.
Product description epBlue™ with MultiCon English (EN) 2.1.2 Assistants Assistants are programs for a specific application. Assistants are optionally available and described in separate operating manuals. The following assistants are available for epBlue: Prep Assistant • Nucleic acid preparation with Eppendorf MagSep kits. After that, a PCR setup can be performed.
Operation epBlue™ with MultiCon English (EN) 3 3.1 Operation Starting and exiting epBlue 3.1.1 Starting epBlue Prerequisites • The device does not show any sign of external damage. Switch on the epMotion using the mains/power switch. Switch on the MultiCon. To start epBlue, double-click on the epBlue symbol on the desktop. Alternatively, open the Windows start menu and select Programs >Eppendorf > epBlue. epBlue is started. The login screen appears. 3.1.
Operation epBlue™ with MultiCon English (EN) Abb. 3-1: epBlue start screen 1 2 3 4 5 Fig. 3-1: epBlue start screen 1 Eppendorf menu Exit the program. 4 Status area epMotion status. 2 Menu bar Contains functions that can be accessed in all applications. 5 Working range Contents and applications. 3 Information area Contains information on the open file. 3.1.4 Logging off as a user You can only log off if all applications started from your user account have been exited.
Operation epBlue™ with MultiCon English (EN) 3.1.5 Exiting epBlue and switching off MultiCon 1. In the Eppendorf menu, select Close. 2. When the program is ended, shut down MultiCon. 3. Switch off the epMotion using the mains power switch. 3.2 Initial steps 3.2.1 Using the touch screen and mouse You can operate the MultiCon by using the touch screen or a USB mouse. Connect the mouse to a USB interface on the MultiCon. Tab.
Operation epBlue™ with MultiCon English (EN) Switch the automatic display of the screen key pad on and off. In the File menu, select the Show keyboard automatically entry. Tab. 3-2: epBlue key combinations Enter special characters. Press the letter key for 2 s. Confirm your entry. Press the key once. Switch between lowercase letters and capital letters. To enter a capital letter, press the key once. Then press the letter.
Operation epBlue™ with MultiCon English (EN) Abb. 3-4: Help drop-down menu Fig. 3-4: Help drop-down menu Device manuals entry Open the operating manual of the device. About epBlue entry Show the epBlue version. Software manuals entry Open the operating manual of the software. 3.2.4 Status area The status area is located in the top right of the screen.
Operation epBlue™ with MultiCon English (EN)
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4 epBlue Application Runner - Carrying out the application This chapter describes how to carry out an application on the epMotion. 4.1 Opening an application Abb. 4-1: Selecting an application Fig. 4-1: Selecting an application Prerequisites • epMotion is ready for operation. • Application Runner is started. 1. Select the application in the Select application area.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4.2 Preparing the application run 4.2.1 Selecting the device Abb. 4-2: Selection of an available device Fig. 4-2: Selection of an available device NOTICE! Tool collisions with incorrectly positioned labware. Labware must be positioned on the epMotion worktable as defined in the application. Tools and labware become damaged in a collision. A collision can lead to sample loss.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4.2.2 Entering the sample quantity Abb. 4-3: Process control window Fig. 4-3: Process control window The window Process control appears if the sample quantity in the application is variable. 1. Enter the sample quantity for the current run. 2. Press the OK button. 4.2.3 Setting the HEPA air filter and the UV lamp Abb. 4-4: Setting the parameters for the HEPA air filter and the UV lamp Fig.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4.2.4 Placing the labware on the epMotion worktable Position the labware on the epMotion worktable according to the application. Additional information on positioning the labware can be found in the hardware operating manual. 4.2.5 Setting the run parameters for the optical sensor If you have the epBlue ID module, the Use bar codes checkbox will appear in the window.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 1. Set the functions of the optical sensor. 2. Activate ID tracking, if required. 3. Press the Run button. 4.2.6 Setting the ID tracking parameters If you have the epBlue ID module and ID tracking is activated for the labware, a window appears. In the labware settings, you defined whether the IDs are entered manually or read in via the entry list. The window displays the corresponding option. 4.2.6.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 5. Confirm your entry with the OK button. 6. Press the Next button to enter the ID for the next labware. 7. When all IDs have been entered, the application starts. 4.2.6.2 Reading the IDs with an entry list Prerequisites • Labware ID tracking is activated. • The Entry list option is activated for the labware. 1. Press the Next button. You are prompted to enter the IDs for the labware.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4.2.7 Entering the filling level of the labware 4.2.7.1 Checking the filling level automatically If the Detect volumes function is active, the optical sensor checks the filling level after the start. If the following problems occur, a dialog window appears: • Filling level too low. • Filling level too high. • Filling level cannot be correctly detected. Abb. 4-7: Checking window Fig.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4.2.7.2 Entering the filling level manually If the Detect volumes function is not active, you have to enter the filling level manually after the start. Abb. 4-8: Entering the filling level Fig. 4-8: Entering the filling level Labware info area Information on the selected labware. Liquid detection area Optical sensor settings for the selected labware. Off The filling level is not checked.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) Tab. 4-1: Meaning of the columns in the Volume table Column Meaning Red border Entry of an invalid volume. Invalid volumes are volumes that are smaller than the required volume or larger than the maximum volume of the labware. Position Position of the labware on the worktable. Minimum volume Volume that is required for this run. Start Volume Actual volume in the labware.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4.3 Starting the application Prerequisites • The labware is positioned on the epMotion worktable. • epMotion is ready for operation. • The application run was prepared. (see p. 26) Click on the Run button. The application starts. 4.4 Controlling the application Abb. 4-9: Running application Fig. 4-9: Running application Carrying out the application. The epMotion executes the application until the end.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN) 4.5 Ending the application When the application run is finished, a message appears. Abb. 4-10: The application is finished Abb. 4-11: The application was canceled Fig. 4-10: The application is finished Fig. 4-11: The application was canceled 1. To confirm the message press the OK button. 2. In the Application Runner window, press the Next button. The final window appears.
epBlue Application Runner - Carrying out the application epBlue™ with MultiCon English (EN)
epBlue LogViewer - Managing logs epBlue™ with MultiCon English (EN) 5 epBlue LogViewer - Managing logs The chapter also describes how to view and print application logs. 5.1 Displaying protocols Abb. 5-1: Logs window Fig. 5-1: Logs window Prerequisites • The application was executed (see p. 34). • The epBlue start screen is shown. 1. Click on the LogViewer symbol. Select the folder in which the application is saved. 2. Select the log. The log is opened.
epBlue LogViewer - Managing logs epBlue™ with MultiCon English (EN) 5.2 Saving the protocol as a PDF file Prerequisites 1. Click on the symbol. The print preview opens. 2. Check the layout and set up the page. 3. Save or print the document as a PDF file. 4. Close the window. Tab. 5-1: Symbols in the print preview Search in the document. Show the first page. Print the document Show the previous page. Page setup. Show the next page. Scale the document. Show the last page. Zoom in the view.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6 epBlue Application Editor - Creating and editing applications This chapter describes how to create and edit applications in epBlue. 6.1 Overview Applications are programmed processes in epBlue. An application consists of equipping the worktable and a sequence of commands. Each command triggers a specific action. Applications are created in a fixed order: 1. Create an empty application (see p. 40). 2.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.2.2 Creating an empty application Abb. 6-2: Start screen of the Application Editor Fig. 6-2: Start screen of the Application Editor Prerequisites • The Application Editor is open. 1. Select the folder in which the application is to be saved. 2. Click on the symbol in the menu bar. The Create new application window appears. epBlue detects the connected device. 3.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.2.4 Saving an application To save an application, click on the symbol in the toolbar. To save an application under a new name, click on the To save all open applications, click on the symbol in the toolbar. symbol in the toolbar. 6.2.5 Positioning labware 6.2.5.1 Positioning the labware on the epBlue worktable Labware can only be positioned at specific locations.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Prerequisites • Labware combinations have been created for reaction vessels (see p. 92). • The application is open (see p. 25). • The Worktable window is open. 1. Select labware from the Labware area. 2. Draw the labware to the desired position of the epBlue worktable. If the labware cannot be positioned at this location, the location is marked in red. 6.2.5.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) • • • • • • EP_DWP_2000 EP_DWP_1000 EP_DWP_500 EP_MTP_384 EP_MTP_96 EP_MTP_UV_VIS_96 On the vacuum unit, you can stack 4 labware components. Information on the vacuum unit can be found in the hardware operating manual. Plates that are stacked may only be filled up to the working volume. Abb. 6-4: Stacking labware on the epBlue worktable Fig.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.2.6 Defining the application parameters of the labware You can change the properties of each labware used. Abb. 6-5: Properties window Fig. 6-5: Properties window To change the properties of a labware, double click on the labware. Alternatively, select the labware and go to the Properties menu with the right mouse button or click on the Properties button. The Properties window appears.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.2.8 Defining procedures A procedure is a defined sequence of commands that epMotion executes during an application. Commands are described in the Commands chapter (see p. 47). The first command of each procedure is the Procedure Start command. The Procedure Start command is created automatically and cannot be deleted. The Procedure Start command shows the epBlue worktable at the start of the application.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Prerequisites • The application is open (see p. 25). • Labware is positioned on the worktable (see p. 25). • The Procedure window is open. 6.2.8.1 Inserting a command 1. Select a command from the Commands column. 2. Drag the command to the Procedure column. 3. In order to insert a command at the end of the application, double-click on the selected command in the column Commands. 6.2.8.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.2.9 Checking an application 1. Click on the symbol in the menu bar. 2. Select the Check command function. The application is checked. Example: Pipette tips without a filter are placed on the epMotion worktable. In the application, pipette tips with filter were selected. The screen displays an error message. 6.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Additional commands BAR Vacuum epMotion with vacuum unit. 6.3.1 Sample transfer command Various transfer commands are available to transfer liquids. All transfer commands are derived from the Sample transfer command. Abb. 6-7: Principle of the Sample transfer command Fig.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.1.1 Sample transfer > Parameter command Abb. 6-8: Sample transfer > Parameter Fig. 6-8: Sample transfer > Parameter Volume Select volume in µL or nL. A decimal place is available for volumes ≤ 99.9 µL. You can transfer a volume larger than the maximum volume of the pipette tip. The volume to be transferred will be pipetted in several steps.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.1.2 Sample transfer > Options command Abb. 6-9: Sample transfer > Options Fig. 6-9: Sample transfer > Options Aspirate from bottom Aspirate liquid from the base of the vessel. When using several vessels with different volumes, enter the lowest filling volume of these vessels (Sample transfer > Parameter). Elution from filter Aspirate liquids from the supernatant in filter plates.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Reuse tips Returned pipette tips will be reused. The pipette tips will be reused within an application for the same source vessel. Is only executed if the restore tips option was selected in a previous command and the restored tips are available. The Reuse tips checkbox is only available with specific settings of the Change Tip option.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Dispense Distance between the pipette tip and base of the vessel during dispensing. If the dispensing position is above the vessel, the distance will be corrected to the height of the vessel. Information on mixing liquids can be found in the hardware operating manual. NOTICE! Contamination of the dispensing tool due to high mixing speed.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Liquid type Optimized for 50 µL tips: pipetting as of 50 µL tips: Remarks dispensing as of Protein Water with 1 % albumin (10 g/L), 0.01 % Triton X-100 5 µL 5 µL • New pipette tips are pre-wetted with the liquid to be aspirated. Prewetting option is set (see p. 54). • The bending of the surface of the liquid has a negative impact on the free jet capability of dispensing in cell culture plates.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Liquid type Optimized for 50 µL tips: pipetting as of 50 µL tips: Remarks dispensing as of Speed_xl Demineralized water. 1 µL 3 µL • Mixing at high dispensing speed. • High dispensing speed poses a high risk of contamination for small vessels. • Good mixing in DWP 96 with 750 µL aspiration and 750 µL dispensing. Speed_xs Demineralized water. 1 µL 3 µL • For slow aspiration from filter plates.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Enter the dispensing speed of the liquid in the Dispense speed field. Input range: 0.2 mm/s – 110 mm/s. Select a low dispensing speed if you dispense larger volumes in empty vessels. A lower dispensing speed decreases the risk of liquid spraying back. Use larger vessels to achieve better mixing results due to a higher speed. Enter the blow-out delay in the Blow delay field. Input range: 0 ms – 99999 ms.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.1.6 Testing the changed liquid type Prerequisites • The parameters of the liquid types are customized to your requirements. 1. Carry out the sample run of the application with demineralized water. 2. Check the precision and accuracy of the dispensing. 3. Check the working volume of the labware. No liquid may spray out of the labware. 4.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.3 Dilute command The Dilute command is used to create serial dilution series, e.g., for PCR standards. A defined volume is pipetted several times from one vessel to the next. Abb. 6-13: Principle of the Dilute command Undiluted Sample Diluent Diluent Diluent 1. Dilution 2. Dilution 3. Dilution Fig.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.3.1 Example of a dilution series This example explains the concept of a dilution series. The example does not describe the full application. Objective • 24 samples are in a rack with 24 positions and should be diluted 1:1000. • The dilution should be completed in 3 program steps with dilutions of 1:10. • First, a single-channel dispensing tool is used.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Transferring diluent to the destination labware 2. Pattern of the Reagent transfer command: With an eight-channel dispensing tool, the Reagent transfer command is used to aspirate the diluent from the 300 mL reservoir and dispense it into the empty wells of the 96-well plate. Carrying out the dilution 3.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.4 Pool command The Pool command can be used to combine liquids from several source positions in one or more destination positions. Abb. 6-14: Principle of the Pool command Fig. 6-14: Principle of the Pool command The windows and functions of the Pool command are identical with the Sample transfer command (see p. 48).
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.6 Comment command Abb. 6-15: Comment command Fig. 6-15: Comment command Comment field Enter comment A comment can be output using the Comment command. When the command is executed, the comment will be displayed in the command list of the application runner.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.7 Exchange command The command Exchange only is available for epMotion 5070. Abb. 6-16: Exchange command Fig. 6-16: Exchange command Labware drop-down menu Labware taken out of the epMotion Exchange with drop-down menu Labware to be placed inside the epMotion The Exchange command can be used to exchange labware. When the command is executed, epBlue prompts you to replace the labware manually. 6.3.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) If the Magnetic separation command is executed when the Separation On checkbox is checked, the magnetic fingers will be drawn out. During the waiting period, the magnetic beads will be separated by the drawn out magnetic fingers on the tube inner wall. After the waiting period has elapsed, the next command in the procedure will be executed.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.10 Number of samples command The Number of samples command is used to define how many samples or positions will be processed in the following transfer commands. The Number of samples command is valid until it has been replaced by a new Number of samples command. You can use several Number of samples commands within one application.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) • Before the Dilute command: number of samples to be diluted. The number of dilution steps is defined by the pattern. • Before the Mix command: number of source positions in which liquid is mixed. • With an eight-channel dispensing tool: for values between 1 – 8, 8 samples are processed. For values 9 - 16, 16 samples are processed, etc. 6.3.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.12 Thermomixer command The command Thermomixer only is available for devices with thermomixer. The Thermomixer command can be used to set the temperature and speed of the thermomixer. Abb. 6-20: Thermomixer window Fig. 6-20: Thermomixer window Template drop-down list The templates for the labware contain saved settings for mixing.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Tab. 6-2: Maximum speed for labware on the thermomixer Labware Maximum speed in rpm Tubes 1000 DWP 96 1200 DWP 384 1200 MTP 6 1000 MTP 96 2000 MTP 384 2000 PCR 96 2000 PCR 384 2000 Reservoir Rack Cannot be mixed Thermorack/Thermoblock 1000 Thermoadapter 96 1000 Thermoadapter 384 1000 Thermorack TMX 1300 PrepRack 1300 6.3.12.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.13 Vacuum command BAR The command Vacuum only is available for devices with vacuum unit. The Vacuum command is used to create a vacuum in the vacuum unit. You can aspirate liquid in filter plates. Prior to executing the application, equip the vacuum unit as described in the epMotion hardware operating manual. Abb. 6-22: Vacuum window Fig.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.13.1 Transporting the Vac Lid with the gripper Use the Vacuum command to transport the Vac Lid from location T0 to the filter plate in the vacuum unit and back to location T0 with the gripper. Prerequisites • The Vac Lid is in location T0. To have the Vac Lid transported from location T0 to the filter plate at the start of the Vacuum command, activate the Use Vacuum Lid checkbox.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.15 User intervention command The User intervention command is used to interrupt an application. The application will be continued when the user confirms a message. You can use the User intervention command to empty the waste container manually. Do not change the filling volume during the interruption. If you need to change the filling volume, use several applications to do so.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Abb. 6-25: Wait window Fig. 6-25: Wait window Duration field Waiting period. Wait for temperature checkbox The waiting period begins after the thermal module has reached the set temperature. 6.3.17 Location drop-down list Thermal module whose temperature control is waited for. Importing commands from a CSV file A CSV file is an ASCII text file. A CSV file defines the structure and contents of a table.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.17.1 Creating a CSV file You can create and edit CSV files using a spreadsheet software or text editor. Make sure that the table meets the following criteria: • Each transfer command is defined in a separate row, (Fig. 6-26 on p. 73). In the screenshot, these are the cells A1 to E1. The names can be abbreviated and also apply to plates.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) The tools for the CSV file are coded using numbers: – 1 equals TS_50 – 2 equals TS_300 – 3 equals TS_1000 – Eight-channel dispensing tools cannot be used. Abb. 6-26: CSV file in a spreadsheet software Fig. 6-26: CSV file in a spreadsheet software Abb. 6-27: CSV file in a text editor Fig.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.3.17.2 Importing a CSV file Prerequisites • CSV file available (see p. 72). A maximum of 500 transfer commands can be imported. Import a CSV file as follows: 1. Create a new application. 2. Insert the Number of samples command as the first command. 3. In the Number of samples command, activate the Fixed sample count checkbox. 4. Set the Number of samples parameter to 1. 5.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 7. Define settings used in the CSV file in the Options and Mix fields. The Elution from filter option is not available for imported commands. The mixing volumes and mixing frequencies must be suitable for all imported transfer commands. The value set for the mixing frequency must be manually overwritten with a different value. If you use different dispensing tools, use a mixing frequency of 11 mm/s. 8.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.4 Pattern For the pattern, enter the source position and destination position between which liquid transfers take place. A pattern can contain 4 source labware and 4 destination labware. Type of pattern Description Regular pattern • • • • Available for all commands. With regular pattern. epBlue detects and completes the pattern. Not available for ReservoirRacks and ReservoirRack modules.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.4.1 Menu bar in the Edit Pattern window Abb. 6-28: Menu bar in the Edit Pattern window Fig. 6-28: Menu bar in the Edit Pattern window Reset Reject pattern. Back arrow One step back. Complete Preview If the pattern is regular, the pattern is completed Preview of the pattern with an increased number until the number of samples defined in the of samples. Number of Samples command is reached.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) Abb. 6-29: Selecting the first source position Abb. 6-30: Selecting the first destination position Fig. 6-29: Selecting the first source position Fig. 6-30: Selecting the first destination position 7. Select the next source position in the source labware. 8. Select the next destination position in the destination labware. Abb. 6-31: Selecting the next source position Fig.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.4.3 Defining irregular patterns Irregular patterns can be defined for source labware and destination labware. If the pattern is irregular, it is not completed. Prerequisites • Labware is positioned on the worktable. • Create the application with the Transfer or Mix command (see p. 48). 1. Press the Pattern button. Abb. 6-33: Edit Pattern window Fig.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.5 Optical sensor The hardware operating manual contains a description of the of the functioning of the optical sensor. The following functions of the optical sensor can be activated: • Detect volumes Determines the filling level for positions of labware, which has the Detect volumes option activated. • Detect tips Checks to see if the pipette tips defined in the application are available.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.5.1.1 Setting application parameters for the Detect volumes function Abb. 6-34: Properties window Fig. 6-34: Properties window Tab. 6-5: Detect volumes Off • Deactivate the optical sensor. • The volume of all vessels must be entered when the application is started. For labware with ≤ vessels, the volume for each vessel can be specified in the Volume column.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.5.1.2 Setting the run parameters for the Detect volumes function Abb. 6-35: Run parameter settings at the start of the application Fig. 6-35: Run parameter settings at the start of the application Detect volumes radio button Activate the Detect volumes function. The parameters set in the application for the labware are used. Detect tips checkbox Activate Detect tips.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN) 6.5.2 Detect tips You can only use the Detect tips function, if you are using ep.T.I.P.S. Motion in tip holders and racks by Eppendorf. Enter the Detect tips function as a run parameter at the start of the application (see Setting the run parameters for the optical sensor on p. 28). The Detect tips function checks the type of pipette tips and determines the number and position of tips in the rack. The epT.I.
epBlue Application Editor - Creating and editing applications epBlue™ with MultiCon English (EN)
epBlue Application Editor - Managing applications and folders epBlue™ with MultiCon English (EN) 7 epBlue Application Editor - Managing applications and folders This chapter describes how to manage applications in epBlue. Folders are managed according to the same rules. 7.1 Menu bar and start screen Abb. 7-1: Application Editor menu bar Fig.
epBlue Application Editor - Managing applications and folders epBlue™ with MultiCon English (EN) 7.2 Copying and deleting applications 7.2.1 Copying applications You can copy applications. The Eppendorf folder contains applications defined by Eppendorf AG. These applications cannot be executed or changed. They are used as templates and program examples. Copy these applications to your area in order to modify and execute it. Prerequisites • The start screen in the Application Editor is open. 1.
epBlue Application Editor - Managing applications and folders epBlue™ with MultiCon English (EN) Abb. 7-3: Edit Properties window Fig. 7-3: Edit Properties window Change the application settings. To save the changes, press the Save button. 7.4 Importing and exporting applications 7.4.1 Importing applications Tab. 7-1: You can import applications in the following file formats: File format Source of file *.ws or *.lhs Applications from the control panel *.
epBlue Application Editor - Managing applications and folders epBlue™ with MultiCon English (EN) Abb. 7-4: Import Application window Fig. 7-4: Import Application window Name area List of all applications for which import was selected. Standard application checkbox Activate if the application is to be imported. Selected checkbox Activated, if the application is an Eppendorf template. Materials tab Shows the labware used in the applications. Import button Import applications.
epBlue Application Editor - Managing applications and folders epBlue™ with MultiCon English (EN) 7.4.2 Exporting applications You can export applications individually. You can export a folder with applications. Prerequisites • The start screen in the Application Editor is open. 1. Select the application to be exported. 2. Click on the symbol in the menu bar. The Browse For Folder window opens. 3. Select the folder in which the application is to be saved. 4. Press the OK button.
epBlue Application Editor - Managing applications and folders epBlue™ with MultiCon English (EN) 7.5 Saving an application as a PDF file You can save an application as a PDF file or print the application. An application includes the procedure and equipping of the worktable. You can save the log of an application as a PDF file or print the log (see p. 38). Prerequisites • A printer is connected to the MultiCon. • The application is open. 1. Click on the symbol. The print preview opens.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8 epBlue Labware Editor - Creating and managing labware This chapter describes how to create and manage labware and labware combinations in epBlue. Information on properties and use of labware can be found in your hardware operating manual. 8.1 Overview of labware The name of the labware includes information on the manufacturer and type. Example: EP_MTP_384V stands for Eppendorf_microplate_384 wells in V-Form.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.2 Managing labware 8.2.1 Menu bar Abb. 8-1: Menu bar in the Labware Editor Fig. 8-1: Menu bar in the Labware Editor Create new labware Display labware properties Open labware Import labware Delete labware Export labware Abb. 8-2: Start screen of the Labware Editor Fig.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.2.2 Deactivating and activating labware To keep a clear overview of your labware library in the Application Editor, you can deactivate labware that is not being used. Deactivated labware can be reactivated at any time. Applications with deactivated labware cannot be started. If you start an application with deactivated labware, an error message appears. Proceed as follows: 1. Open the Labware Editor. 2.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) Abb. 8-3: Import labware window Fig. 8-3: Import labware window Name area List of the labware selected for import. Define import rules area Define rules for the import of the labware. Standard labware checkbox Activated, if the labware is standard Eppendorf labware. Import button Import labware. Selected checkbox Activate if the labware is to be imported. Cancel button Cancel import. 4.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.2.5 Export labware You can export labware files in order to use them for another epMotion. You can export individual labware files or the content of a folder. Exported applications can only be used with epBlue from version 40.x. Proceed as follows: Prerequisites • The Labware Editor is open. 1. Select labware or folders. 2. Click on the symbol in the menu bar. Abb. 8-5: Export labware window Fig.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.2.6 Viewing the properties of the labware Proceed as follows: Prerequisites • The Labware Editor is open. Select the labware. The properties of the labware are displayed in the Properties column. 8.2.7 Setting the bottom tolerance NOTICE! Material damage due to placing the pipette tip on the base of the vessel. If the pipette tips touch the bottom of the tube, the bottom tolerance is too low.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) Name field Name of labware Remain Volume field Remaining volume will be calculated. Comment field Description of labware Eppendorf Standard Labware checkbox This checkmark is placed automatically and cannot be removed. Eppendorf Standard Labware is write-protected. Bottom Tolerance field Bottom tolerance. The lowest bottom tolerance is 200 µm. Save button Save changes. 3.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.3 Creating labware combinations Before you create an application, you must create the required labware combination and save it in your labware library. You have the following options: • Equipping racks, thermoracks or Reservoir Rack modules with individual vessels. • Equipping Reservoir Racks with reservoirs or Reservoir Rack modules. • Creating a labware combination based on existing labware. 8.3.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 1. Click on the symbol in the menu bar. The Create new labware window opens. 2. Enter the name, description and labware combination. Racks/ thermoracks + tubes Equip the rack and thermorack with individual vessels. Modules + tubes Equip the ReservoirRack Modul with reaction vessels. ReservoirRack 7 Equip the ReservoirRack 7 with ReservoirRack modules.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.3.2 Creating a labware combination based on existing labware Abb. 8-9: Start screen of the Labware Editor Fig. 8-9: Start screen of the Labware Editor Prerequisites • The Labware Editor is open. 1. Select the labware. The Properties column displays information, properties and detection limits of the labware. 2. Click on the symbol in the menu bar. The Edit Properties window appears.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) Abb. 8-10: Edit Properties window Fig. 8-10: Edit Properties window 3. Edit the properties of the labware. 4. Enter a new name in the Name field. 5. Press the Save button.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.3.3 Equipping the racks, thermoracks and Reservoir Rack modules with individual vessels Abb. 8-11: Creating a labware combination Fig. 8-11: Creating a labware combination Racks column Racks and Reservoir Rack modules. If you have selected a rack, only vessels that can be combined with the rack will be shown. Clear button Delete the selected individual vessel, rack, ReservoirRack module.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) Abb. 8-12: Rack and vessel as labware combination Fig. 8-12: Rack and vessel as labware combination 3. To save the labware combination, click on the symbol in the menu bar. The labware combination is saved in the labware library. 8.3.4 Equipping the Reservoir Rack with reservoirs and Reservoir Rack modules Abb. 8-13: Equipping ReservoirRacks Fig.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) Available tubs column Reservoirs and equipped Reservoir Rack modules. Vendor Info field Properties of the selected labware. Save button Save the changed combination. Save as button Save the new combination. Proceed as follows: Prerequisites • The ReservoirRack modules were created with the Module Rack + Tubes option. • The labware is open. 1.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.4.2 Requesting labware definitions Eppendorf creates definitions for labware that you would like to use in the epMotion. You can record these definitions in the epBlue labware library. 1. Check to see if the definition for your labware is in the epBlue labware library. 2. Check to see if the definition for your labware is in the labware library on the www.eppendorf.com/ automation webpage. 3.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.5.4 PCR plates Labware types in epBlue: Plates > PCR. Labware Labware types PCR plates with 96 wells PCR 96 PCR plates with 384 wells PCR 384 8.5.5 Filter plates Plates in the labware library can be found under Plates. Labware Labware types Filter plates with 96 wells Filter Plates 96 Filter plates with 384 wells Filter Plates 384 8.5.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.5.10 Reservoir Rack Module TC First, equip a Reservoir Rack module with vessels. Then place the equipped ReservoirRack module in the ReservoirRack. The software only positions equipped Reservoir Rack modules in a Reservoir Rack. Labware types in epBlue for unequipped ReservoirRack modules: Racks with Tubes > ThermoRacks + Modules.
epBlue Labware Editor - Creating and managing labware epBlue™ with MultiCon English (EN) 8.5.13 Thermoadapter Labware types in epBlue: Adapter > Thermo In the software you can find the Thermoadapter LC Sample+Cartridge under Thermoblocks with plates. The thermoadapter and plate combination is created during the editing of the worktable or via a transport command. With the "Thermoadapter with skirted PCR plate" combination, the plate can be transported by the gripper.
epBlue simulation - Simulating applications epBlue™ with MultiCon English (EN) 9 epBlue simulation - Simulating applications This chapter describes how to simulate applications in epBlue. 9.1 Simulating the application Before starting your application, you can simulate the run. When you start a simulation, no other simulation can be open. 9.1.1 Starting a simulation Prerequisites • epBlue is started. 1. Click on the Applicaton Simulator symbol on the start screen. 2.
epBlue simulation - Simulating applications epBlue™ with MultiCon English (EN) 9.1.2 Controlling the simulation Abb. 9-1: Simulation window Fig. 9-1: Simulation window Prerequisites • The simulation has started. 1. You can control the simulation with the following symbols: Tab. 9-1: Symbol Symbols for controlling a simulation Meaning Set the speed of the simulation. You can set a speed from 1 – 10x. The figure 1 corresponds to real time. The figure 10 means 10 times faster than real time.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN) 10 epBlue ID - Working with ID tracking This chapter describes the basics of ID tracking. The chapter describes how to manage ID lists. 10.1 ID lists 10.1.1 Overview The path of the samples is tracked and documented in the ID list during an application. An ID list refers to a single labware. An ID list contains the IDs of the locations in the labware and description of these locations.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN) 10.1.2 Input lists and result lists Input list • ID list with information on source labware. • The input list contains the IDs of the source positions. • epBlue creates an input list for each source labware for which ID tracking is active. • When using reagents with two IDs, e.g.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN) 10.3 Reading IDs into epBlue IDs can be entered manually when starting an application. To do so, enter the IDs by hand or read them using the barcode reader. Alternatively, you can use an ID list as an entry list. Tab. 10-3: Differences when entering IDs Labware Enter IDs manually Use the entry list Plate One ID per plate. One ID per plate. One ID per used well. Rack The rack does not have an ID. The rack does not have an ID.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN) 10.4 Managing ID lists Abb. 10-1: ID List Management window Fig.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN) 10.4.2 Filtering and sorting ID lists You can filter ID lists by date, user, format or type of creation. You can filter ID lists by name, list ID and date of creation. Prerequisites • The ID List Management window is open. • The List view has been selected. 1. Select the criteria by which the ID lists are to be filtered. Click on the corresponding drop-down list and select the criterion. 2.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN) 10.4.4 Export ID lists Users of the Admin Lab, User Level 2 and Administrator user groups can export ID lists. Input lists and result lists can be exported in the formats CSV and XML. These ID lists can be used for other applications. Prerequisites • The ID List Management window is open. 1. Click on the symbol in the menu bar. 2. Select the ID list. 3. Press the Export ID lists button. 4.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN) 10.5 Making the basic settings for the epBlue ID In order to make the basic settings for the epBlue ID, you must be part of the Admin Lab user group. Abb. 10-2: ID List Settings window Fig. 10-2: ID List Settings window Show reagent IDs in result list Switch on and off the ID tracking for the Reagent Transfer command. Import/Export default directory Define the default folder for the import and export of ID lists.
epBlue ID - Working with ID tracking epBlue™ with MultiCon English (EN)
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) 11 epBlue settings - Checking components and making the basic settings This chapter describes how to check components of the epMotion and make the basic settings. Prerequisites • The epMotion is ready for operation. • epBlue is started. • The start screen is shown. 1. Click on the Settings symbol on the start screen. 2. Select the function. 11.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) Detect volumes radio button Activate the Detect volumes function. The parameters set in the application for the labware are used. Detect tips checkbox Activate the Detect tips function. Check labware placement checkbox Activate the Check labware placement function. Use required minimum volumes radio button epBlue assumes that all vessels are filled with the minimum required volume for this run.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) 11.3.1 Lock the tool 1. Slide the tool into the tool holder from below. 2. Hold the tool tightly At the same time, press the Lock button. The tool holder locks the tool. 11.3.2 Unlocking the tool 1. Hold the tool tightly. At the same time, press the Unlock button. The tool holder unlocks the tool. 2. Remove the tool. 11.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) 11.5 Gripper The Gripper function can be used to open and close the gripper. Labware can be removed from the gripper if necessary. Abb. 11-4: Gripper function Fig. 11-4: Gripper function Distance field Distance of gripper arms Set button Confirm new distance of gripper arms Gripper distance (µm) field New distance of gripper arms Export field Exports data 11.5.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) 11.6 Temperature control unit X The Temperature control unit X function allows you to check the epMotion thermal modules. Abb. 11-5: Temperature control unit function Fig. 11-5: Temperature control unit function Desired Temperature Shows the set temperature of the thermal module. Remain time Shows the time remaining until the end of temperature control (in min).
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) 11.7 Thermomixer You can use this function to check the thermomixer. NOTICE! Contamination of samples and the device due to high mixing frequency. If the speed is set too high, liquid will spray out of the vessels. Labware may become loose from the holder and fly about. Observe the maximum speeds of the labware in the operating instructions. Tab.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) RPM field Speed of the thermomixer in rpm. Start button Stop button Time field Duration of the mixing process. Remaining time field Remaining time in the mixing process. 1. Define the speed and temperature in the Parameter area. 2. To check the mixing function, press the Start button. The thermomixer mixes until the time is elapsed or until the test has been exited using the Stop button. 11.7.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) The current status will be displayed in the State area. You can make settings in the Set Temperature area. 1. Enter the set temperature in the New Temperature field. 2. Enter the mixing time in the New time field. 3. Press the Start button. The thermomixer tempers the labware to the set temperature.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) 11.9 UV lamp The UV lamp is used to decontaminate the epMotion worktable. An irradiation cycle lasts 15 min. 11.9.1 Decontaminating the worktable CAUTION! Risk to health due to UV radiation. UV radiation causes eye and skin damage. UV radiation may escape outward through the ventilation slits in the rear panel of the epMotion.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) Abb. 11-9: Lamp symbol in status area Abb. 11-10: UV Light button Fig. 11-9: Lamp symbol in status area Fig. 11-10: UV Light button The tool holder moves. The UV lamp will be switched on. The UV Light button shows the remaining irradiation period. The lamp symbol appears in the status area. 11.10 HEPA air filter Air is drawn in using a fan. This aspirated air is filtered by the HEPA air filter.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN) Abb. 11-11: Filter symbol in the status area Fig. 11-11: Filter symbol in the status area The HEPA air filter is switched on. The filter symbol appears in the status area. The HEPA air filter remains switched on if you exit the HEPA filter function. Now start your application. 11.10.2 Switching off the HEPA air filter Prerequisites • The HEPA air filter is running.
epBlue settings - Checking components and making the basic settings epBlue™ with MultiCon English (EN)
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN) 12 epBlue settings - Creating and managing users This chapter describes how you can create and manage users as an administrator. The chapter describes how to change your password as a user. 12.1 Managing the user accounts as an administrator 12.1.1 Logging in as administrator in epBlue Prerequisites • epBlue is started. • epBlue displays the login screen. 1. Enter the user name Administrator. 2.
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN) Group column Available user groups. Fixed checkbox The checkbox is activated. The user rights of a group cannot be changed. System, Application, Labware … columns Groups of user rights. Active rights are marked with an X for each user group.
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN) 12.1.3 User accounts 12.1.3.1 Overview The Account > Overview tab displays a list of all user groups for epBlue. Here you can see which users have user accounts in epBlue. Abb. 12-2: Overview of user accounts Fig.
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN) 12.1.3.2 Creating a user account Create a user account for each user. Each user name can only be used once. Deleted user names cannot be used again. Abb. 12-3: Creating a user account Fig. 12-3: Creating a user account Prerequisites • epBlue is started. • You are now logged in as administrator. • The Settings area is open. 1. Select the Account > Edit tab. 2. Select Create Account. The Create Account window appears. 3.
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN) 12.1.3.3 Editing a user account Abb. 12-4: Account > Edit tab Fig. 12-4: Account > Edit tab Prerequisites • epBlue is started. • You are now logged in as administrator. • The Settings area is open. 1. Select the Account > Edit tab. 2. Select the user in the Account list. The user account opens. 3. Change the entries in the fields. 4. Press the Submit button. The changes are saved. 12.1.3.
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN) 4. epBlue asks whether you would like to delete the user account. To delete the user account, click on Yes. If you do not want to delete the user account, click No. 12.1.4 Assigning a new password for a user account If the user has forgotten his password, the administrator can assign a new password. Prerequisites • epBlue is started. • You are now logged in as administrator. • The Settings area is open. 1.
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN) Abb. 12-5: Changing a password Fig. 12-5: Changing a password The Account window appears. 4. In the Old Password field, enter the current password. 5. Enter the new password into the New Password and Confirm password fields. If the entries differ, an error message appears. 6. Press the Submit button. The new password is now active.
epBlue settings - Creating and managing users epBlue™ with MultiCon English (EN)
epBlue Settings - Saving data and creating print templates epBlue™ with MultiCon English (EN) 13 epBlue Settings - Saving data and creating print templates This chapter describes how to back-up the data base, create print templates and change the name of your device. 13.1 Data backup epBlue saves applications, protocols, the labware library and information on user accounts in a database. The Backup function can be used to create a backup copy of the database.
epBlue Settings - Saving data and creating print templates epBlue™ with MultiCon English (EN) Destination selection area Location where a backup copy will be saved Details field Information on backup Status field Shows if the software is ready or damaged Backup button Starts the data backup Progress bar Progress of backup Proceed as follows: Prerequisites • epBlue is started. • You are logged in as administrator. 1. Click on the Settings symbol on the start screen. 2. Select Backup.
epBlue Settings - Saving data and creating print templates epBlue™ with MultiCon English (EN) 13.1.3 Restoring the database You can import your backup copy in epBlue. The database will be restored to the status at the time of the backup. All data in epBlue is overwritten when you import the backup copy. Changes made after this backup copy was created will be lost. Abb. 13-2: Restore window Fig. 13-2: Restore window Source Full Backup Location where a backup copy is saved.
epBlue Settings - Saving data and creating print templates epBlue™ with MultiCon English (EN) 13.2 Changing the device name The Device name window contains information on the status and the configuration of the epMotion. The Nickname field contains the name of the epMotion. The serial number will be assigned as the name by default. You can change the name of the device. You can check the settings if you are logged in as an administrator. Abb. 13-3: Device name window Fig.
epBlue Settings - Saving data and creating print templates epBlue™ with MultiCon English (EN) 13.3 Print templates Print templates can define how your print-outs and PDF documents look. Abb. 13-4: Print Header window Fig. 13-4: Print Header window Current preview setting drop-down list Select print templates Show preview button Show a preview Add button Create print templates ...
epBlue Settings - Saving data and creating print templates epBlue™ with MultiCon English (EN) 13.3.1 Create print templates Prerequisites • You are logged in as Administrator or Admin Lab. • epBlue is started. 1. Click on the Settings symbol on the start screen. 2. Select the Print Header function. The Print Header window appears. 3. Click on the Edit print header template button. 4. Enter your company's address in the Company Information field. 5.
epBlue - Software error messages epBlue™ with MultiCon English (EN) 14 epBlue - Software error messages This chapter contains an overview of the software error messages, causes and remedies. If an error occurs, first try the following: 1. Switch off epMotion. 2. Restart epMotion. Execute the application again. If the error occurs again, contact Eppendorf Service or your dealer. The addresses can be found on our webpage www.eppendorf.com.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x0C02 Volume too small for this tool Volume to be dispensed is too Call local Eppendorf small for the selected tool. Application. Possible causes: • Errors in tool files. • Errors in liquid type files. 0x0C08 Tool dimension unknown Tool dimension values unknown. Labware outdated or corrupt. Make sure all labware is of the latest version.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x128E Carrier: steps lost in y • Carrier collision. • Carrier mechanics out of order. Ensure that the worktable Carrier: steps lost in z • Carrier collision. • Carrier mechanics out of order. Ensure that the worktable Carrier: steps lost in z before picking up tip • Tip was still on pipette tool when tool started to pick up a new tip. • Tip rack not placed correctly on the worktable.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x129A Tip too small Reagent Transfer: Used tip is too small. Use a larger tip. 0x129B Source vessel too small Reagent Transfer: Used source Use a larger vessel. vessel is too small. 0x12A2 Check levels in vacuum command: Wells in filter plate not empty Wells of the filter plate plug while applying vacuum.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x12B8 Temperature unit in location C2 too hot for labware (temperature command in this method) Select a labware item that The temperature of location is more resistant to high C2 (according to a temperature temperatures (e.g., made command in this application) from polypropylene or is too high for the labware polycarbonate). placed on this location.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x12D1 Parameter conflict: Elution volume too large for destination tube or well Option elution from filter: volume is too large for the vessel used. Select a tool large enough 0x12E1 Parameter conflict: Pre-wetting not possible when aspirate from bottom is selected See error message. Change application.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x12F7 Waiting for thermomixer Wait until thermomixer function has ended. 0x12F8 The selected The mixing speed is not mixing speed is not allowed for the selected possible with this labware. labware Select another labware or mixing speed. 0x1500 Too big vessel A tube is to be accessed for index in location: ... which the index is greater than the number of tubes on the plate/rack/holder.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause 0x150D Optical sensor: Plate could not be found in location: ... Optical sensor: Rack could not be found in location: ... 0x150E Optical sensor: The tip rack programmed for Tips could not be this location could not be found in location ... found by the optical sensor; possible causes: • Tip rack not placed onto location. • Problems related to the optical sensor function.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause 0x1515 Tool cannot be used Distance between tip cones of Change application. for rack in location the liquid handling tool does ... not match the distance between vessels (e.g., 24 tubes - rack does not fit the 8-channel tool). 0x1516 No vessel in location: ... Vessels that are needed according to the application are not available on the worktable (vessel/rack combination).
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x151E Detected volume is out of detection range ... Do not fill vessels above 0x151F Normally a system/hardware error (malfunction of the optical sensor); but may also be caused by filling a vessel up to the total vessel height. the specified maximum filling volume. In other cases: Call local Eppendorf Service.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x152A Gripper could not be found See error message. Provide the gripper on the 0x152D Tip too short Select other tips or vessels in the method. Tip does not reach the liquid Select other tips or vessels in the application. level at the beginning or during the course of the liquid handling command.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1701 Liquid volume too large for vessel in location:... Provide less volume in the Total volume supplied in a source vessel is larger than needed or larger than vessel. vessel. Change application. When verifying the total volume needed for the source or destination, take into account the additional aspirated volume when using multidispense mode.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause 0x190E The following tool is not selected in the labware file window: ... Select the respective Edit mode/procedure: labware in the Labware The chosen tool is not File window. You need to available in the labware have the appropriate user collection that was selected rights. If you do not have for your lab. Possible cause for the necessary user rights this error message: The ask your administrator.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1911 The following labware has been changed, so the pattern no longer fits: xxx 0x1A02 The name is already Edit mode/labware: used for another The same name has been labware defined for a different rack or another labware item. 0x1A03 This position is not available for the selected labware Edit mode/worktable: Place the selected labware Certain worktable positions in another location.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1A10 8-channel tool cannot be used for this source rack Edit mode/parameter in Choose another rack or command Sample Transfer: another tool. Source rack does not fit to 8-channel-tool (e.g.: 24-well-plate or tube rack with 24 positions). 0x1A11 8-channel tool cannot be used for this destination rack. Choose another rack or Edit mode/parameter in another tool.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause 0x1A17 Invalid entry for speed aspiration (0.2 ... 110) Edit mode/parameter in Enter a value between transfer command: 0.2 and 110 mm/sec. A value beyond the allowed range has been entered for the speed aspiration parameter. 0x1A18 Invalid entry for speed dispense (0.2 ... 110) Edit mode/parameter in Enter a value between transfer command: 0.2 and 110 mm/sec.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1A22, 0x1A32, 0x1A42, 0x1A52 No source or Edit mode/parameter in the Select source or destination selected affected command: destination, respectively. Source or destination rack has not been selected. 0x1A40, 0x1A41, 0x1A50 8-channel tool cannot be used for this source rack Edit mode/parameter in the affected command: Source rack does not fit the 8-channel tool (e.g.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1A78 Number of tubes not supported Edit mode/pattern: One or more Modules have 3, 5, 6, 7 or more than 8 positions. This is not supported. Choose another rack. 0x1A80 Invalid entry for minutes (0 ... 99) Edit mode/parameter in Enter a value between command Wait: 0 and 99 minutes. A value beyond the allowed range has been entered for the parameter minutes.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1AB1 Invalid entry for seconds (0 ... 59) Edit mode/parameter in Enter a value between command Vacuum: 0 and 59 seconds. A value beyond the allowed range has been entered for the seconds parameter. 0x1AB2 Invalid entry for vacuum pressure (1 ... 90) kPa and (10 ... 900) mbar resp. Enter a value between Edit mode/parameter in 0 and 59 seconds.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1C0D You must clear old Edit mode/pattern: Delete the old pattern by pattern first You tried to change a stored pressing the button new Press "new pattern" pattern before deleting the old pattern. pattern. 0x1C0E You must go forward 0x1C0F See explanation in You may only move Edit mode/pattern: "Cause".
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1C15 Pattern too complex Enter a simpler pattern if 0x1C16 This position is already occupied Edit mode/pattern: The pattern algorithm cannot handle this pattern. Note: See note in error 0x1C0F. Edit mode/pattern: When editing the pattern you have tried to select a certain rack position that is already occupied. possible. In case this is not possible: Call local Eppendorf Service.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x1C20 Pattern for sample transfer: only one position per sample on source Edit mode/pattern for command Sample Transfer: Before selecting a second source position, you have to edit the destination for the first source position.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x2026 Bottom tolerance too small Bottom tolerance too small. Use a bigger value. 0x2101 Tool not defined Parameter Pipet. Tool was not edited in the application. See explanation in "Cause".
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x210D Source rack not defined See explanation in Parameter Source was not edited in the application. "Cause". 0x210E Source rack not The source rack you edited in Edit the rack that you programmed in the edited in worktable the procedure of the procedure as Source in the application has been removed worktable, or edit a from the worktable. different source rack in the application.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x211D Mixing cycles in source not defined Parameter No. of Cycles in a mix procedure was not edited for the source in the application (mix procedure as defined in a command Mix or as part of a liquid transfer command via parameter Options). See explanation in 0x211E Invalid entry for mixing cycles in source (1 ... 99) Enter a number between Entry for the No. of Cycles 1 and 99 for the No.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x2122 Parameter conflict: mixing volume in source too small for this tool Edit Volume and Pipet. Tool The Volume and Pipet. Tool so that Volume is within parameters of a mix procedure the pipette tool's volume for source vessels do not range.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x2127 Mixing volume in destination not defined Parameter Volume in a mix procedure for destination vessels was not edited in the application (mix procedure as part of a liquid transfer command via parameter Options). See explanation in 0x2128 Parameter conflict: mixing volume in destination too large for this tool Edit Volume and Pipet. Tool The Volume and Pipet.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause 0x212D Parameter conflict: 8-channel tool cannot be used for this source rack Edit mode/parameter in liquid Choose another rack or another tool. handling command: Source rack does not fit the 8-channel tool (e.g., 24-well plate or tube rack with 24 positions).
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x2135 Location for destination not defined Edit mode/parameter in command Transport: The destination location for the labware to be transported was not edited. See explanation in 0x2136 Invalid entry for seconds (1 ... 59) Edit mode/parameter in Enter a value between command Wait: 0 and 59 seconds. A value beyond the allowed range has been entered for the seconds parameter.
epBlue - Software error messages epBlue™ with MultiCon English (EN) Code Symptom/message Cause Remedy 0x2171 Parameter conflict: See explanation in the error Multidispense message.
Ordering information epBlue™ with MultiCon English (EN) 15 15.1 Ordering information Accessories Order no. (International) 5075 002.507 5075 002.515 5075 002.523 5075 002.728 5075 002.
Index epBlue™ with MultiCon English (EN) Index Number of samples .......................................... 64 Pool .................................................................. 60 Pool one destination ......................................... 60 Reagent transfer ............................................... 56 Sample transfer ................................................ 48 Temperature..................................................... 65 Thermomixer......................................
Index epBlue™ with MultiCon English (EN) H LH assistant .......................................................... 18 HEPA .....................................................................11 LIMS ..................................................................... 11 HEPA air filter......................................................128 Start................................................................128 Stop ................................................................
Index epBlue™ with MultiCon English (EN) R Rack Equipping.......................................................102 ReservoirRack Equipping with ReservoirRack modules ........103 Equipping with reservoirs..............................103 ReservoirRack module Equipping with individual racks ....................102 Result list.............................................................112 S Screen key pad ......................................................21 T Thermomixer............................
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