Instruction Manual

Evaluation procedure

Eppendorf BioPhotometer

D30

English (EN)

12.5.2.2 Calculation of the molar concentration

Application: calculation of the molar concentration of the nucleic acid from the mass concentration and

relative molar mass. The molar mass is either entered directly or calculated by the device from the entered

number of bases or base pairs per nucleic acid molecule.

Mol

= calculated molar concentration of the nucleic acid. Unit: pmol/mL.

C = nucleic acid concentration calculated from the measurement. Unit: μg/mL or ng/μL.

MM = relative molar mass. Unit: kDa

If the number of bases or base pairs per nucleic acid molecule are entered in More calculations instead of

the relative molar mass, the MM is calculated from the number of the bases or base pairs:

For dsDNA:

For ssDNA, RNA, Oligo:

MM = calculated relative molar mass; unit: kDa

bp = entered number of base pairs per molecule

b = entered number of bases per molecule

•For dsDNA the calculation of the molar concentration is based on the assumption of a

double-stranded nucleic acid. For the ssDNA, RNA and Oligo methods, a single-stranded

nucleic acid is assumed.

• For methods which have been reprogrammed via <New Method> in the Routine main

group, Nucleic acids method group, always double-stranded nucleic acids are assumed

for calculating the molar concentration.

Mol

10u