oPhotometer® nual gEN) manual D30 Register your instrument! www.eppendorf.
Copyright © 2014 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Trademarks Eppendorf®, the Eppendorf logo, Eppendorf BioPhotometer®, Eppendorf μCuvette®, and UVette® are registered trademarks of Eppendorf AG, Hamburg, Germany. Cy® is a registered trademark of GE Healthcare UK Ltd., Buckinghamshire, UK. Trademarks are not marked in all cases with ™ or ® in this manual. This product is manufactured under license to issued U.S.
Table of contents Eppendorf BioPhotometer® D30 English (EN) Table of contents 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2.
Table of contents Eppendorf BioPhotometer® D30 English (EN) 6 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.1 Selecting a method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2 Photometry method description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6.2.
Table of contents Eppendorf BioPhotometer® D30 English (EN) 11 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11.
Table of contents Eppendorf BioPhotometer® D30 English (EN)
Operating instructions Eppendorf BioPhotometer® D30 English (EN) 1 1.1 Operating instructions Using this manual Read this operating manual completely before using the device for the first time. Also observe the instructions for use of the accessories. This operating manual is part of the product. Thus, it must always be easily accessible. Enclose this operating manual when transferring the device to third parties.
Operating instructions Eppendorf BioPhotometer® D30 English (EN) 1.
Product description Eppendorf BioPhotometer® D30 English (EN) 2 2.1 Product description Main illustration Abb. 2-1: BioPhotometer D30: Front view and rear view 2 3 3 ce absorban absorban 1 ce height 8.5 m m 1 2 3 abc 4 def 5 ghi 7 pq rs jkl 8 tuv meth od 6 mno 9 wxyz exit func tion 0 dele te µ % ente r standard blan k sample 10 Fig.
Product description Eppendorf BioPhotometer® D30 English (EN) 2.3 Features The BioPhotometer D30 is a UV VIS photometer for measuring liquids in cuvettes. As the measuring data are collected at fixed wavelengths, the device especially is suited for routine applications in the biomolecular, biotechnological, biochemical and cytological areas in research and development. 2.3.
Safety Eppendorf BioPhotometer® D30 English (EN) 3 3.1 Safety Intended use The BioPhotometer D30 is to be used in molecular biology, biochemistry and cell biology research laboratories. The BioPhotometer D30 exclusively is determined for use in the interior of buildings. All country-specific safety requirements for operating electrical equipment in the laboratory must be observed.
Safety Eppendorf BioPhotometer® D30 English (EN) WARNING! Damage due to UV radiation. Microliter cuvettes, e.g., Hellma® TrayCell (or microliter cuvettes with a similar design) divert the radiation from the light source within the cuvette so the radiation can escape upward when the lid is not closed. Before starting a measurement, ensure that the lid on the microliter cuvette is not open.
Safety Eppendorf BioPhotometer® D30 English (EN) NOTICE! Corrosion from aggressive cleaning agents and disinfectants. Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes. Do not incubate the accessories in aggressive cleaning agents or disinfectants for a longer period of time. NOTICE! Damage to electronic components due to condensation. Condensate can form in the device after it has been moved from a cool environment to a warmer environment.
Safety Eppendorf BioPhotometer® D30 English (EN) 3.4 Information on product liability In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator: • • • • • The device is not used in accordance with the operating manual. The device is used outside of its intended use. The device is used with accessories or consumables which are not recommended by Eppendorf.
Installation Eppendorf BioPhotometer® D30 English (EN) 4 4.1 Installation Preparing installation Keep the transport carton and the packing material for subsequent safe transport or storage. Check the completeness of the delivery using the information in the delivery package (see Delivery package on p. 9). Check all parts for any transport damage. 4.
Installation Eppendorf BioPhotometer® D30 English (EN) 4.4 Connecting the printer 4.4.1 Thermal printer DPU-S445 Prerequisites Software version 3.4.4.0 or higher is installed on the device. Connect the thermal printer DPU-S445 to the USB port for printers. 1. Connect the printer cable with the USB port for printers 4 (see Main illustration on p. 9). 2. Connect the printer cable with the printer. 3.
Installation Eppendorf BioPhotometer® D30 English (EN) DIP SW-2 Meaning 4 (ON) Zero = Normal 5 (ON) International 6 (ON) Character 7 (ON) Set 8 (OFF) = U.S. DIP SW-3 Meaning 1 (ON) Data Length = 8 bits 2 (ON) Parity Setting = NO 3 (ON) Parity Condition = Odd 4 (OFF) Busy Control = XON/XOFF 5 (OFF) Baud 6 (ON) Rate 7 (ON) Select 8 (ON) = 9600 bps 4.
Installation Eppendorf BioPhotometer® D30 English (EN)
Operation Eppendorf BioPhotometer® D30 English (EN) 5 5.1 Operation Overview of operating controls Abb. 5-1: Control panel of the BioPhotometer D30 Fig. 5-1: Key: Control panel of the BioPhotometer D30 Function Keypad: Enter digits and text. Keys 1 to 9 as well as 0: When entering text, next to numbers you also can enter letters and special characters by pressing the key several times. Alternatively, you can switch to a displayed keyboard with the [Keyboard] key.
Operation Eppendorf BioPhotometer® D30 English (EN) Key: Function Exit the current selection for the next higher level. Delete entry. Within a sequence of signs, the sign on the left of the cursor is deleted • Call up selected method or function. • Open the selection list. • Confirm entry or selection. Start standard measurement. Start blank measurement. Start sample measurement.
Operation Eppendorf BioPhotometer® D30 English (EN) 5.1.1 Entering text You can enter texts when assigning method names and result units. Restriction: Only digits, letters and the underscore "_" are allowed for method names. Entry via keyboard: Use the and cursor keys to navigate within the entry field and to change single positions in the name. Softkeys: • [Keyboard]: Display keyboard. • [abc]: Change between upper and lower case letters when making entries with the keypad. • [Save]: Save entered text.
Operation Eppendorf BioPhotometer® D30 English (EN) 5.2 Inserting the cuvette Standard rectangular glass or plastic cuvettes can be inserted in the cuvette shaft: • External dimensions: 12.5 mm × 12.5 mm • Height of light path: 8.5 mm higher than cuvette base • Total height: min. 36 mm The cuvettes must be optically transparent for the respective measuring wavelength.
Operation Eppendorf BioPhotometer® D30 English (EN) 5.3 Summary of the measuring procedure 5.3.1 Preparing the measurement 1. Switch on the device and, if required, the printer. The device performs a self test (taking approx. 1 minute) and displays the method selection. 2. Make ready the cuvettes for the measurements (see Inserting the cuvette on p. 22). 3. Prepare the measuring solutions for measuring the blank values, if required, also the standards and the samples. 4.
Operation Eppendorf BioPhotometer® D30 English (EN) 5.3.2.2 Checking parameters Check the parameter setting. The [Page dn] and [Page up] softkeys allow you to call up the parameter list pages. You can modify and save parameters using [Edit]. 5.3.2.3 Measuring the blank and standards For evaluations without standards (e.g. DNA measurements), this method step is omitted. 1. Start by measuring a blank (blank key). 2. Then measure all standards one by one (standard key).
Operation Eppendorf BioPhotometer® D30 English (EN) 5.3.2.4 Measuring samples The sample key is used for measuring your samples consecutively. Blank results will remain saved for the duration of one series of measurement. However, a new blank measurement always is possible. (The adjacent figure shows a measuring procedure with evaluation via the standard curve and, in addition to the sample result, the graph of the standard evaluation.) 5.3.2.5 Finalizing the method 1.
Operation Eppendorf BioPhotometer® D30 English (EN) 5.3.2.7 Printing and exporting 1. Compose data packets for all samples or for selected samples. 2. Print the data, save them to a USB stick or transfer them to a PC via a USB cable.
Operation Eppendorf BioPhotometer® D30 English (EN) 5.3.3 Important measurement instructions Check for each measurement: • For plastic cuvettes: How many consecutive measurements can be reliably carried out in the cuvette? • Measure the cuvette blank value before the sample or standard measurements in order to compensate the cuvette blank value in addition to the reagent blank.
Operation Eppendorf BioPhotometer® D30 English (EN)
Methods Eppendorf BioPhotometer® D30 English (EN) 6 6.1 Methods Selecting a method Methods and method templates are delivered preprogrammed. The methods are organized in main groups and subgroups. Write-protected methods The most important methods in molecular biology. Parameters can be modified, but the modified parameters must be saved under a new method name. Non-write-protected methods You can change parameters any number of times and start the measurement right after saving.
Methods Eppendorf BioPhotometer® D30 English (EN) You can create new methods in all folders using . In Favorites, you can create your own folders (e.g., to allocate folders to specific people), and rename and delete the folders. Tab. 6-2: Softkeys in method selection [Cut] and [Paste] Cut and paste methods. [Copy] and [Paste] Copy and paste methods. [Delete] Delete methods. [Rename] Rename methods.
Methods Eppendorf BioPhotometer® D30 English (EN) Proteins direct UV • Determination of the concentration of proteins via measurement at 280 nm and factor or standard evaluation. • Preprogrammed methods for direct absorbance output as a result (Protein A 280) and for evaluation via albumin-specific absorbance coefficients (Albumin A 280).
Methods Eppendorf BioPhotometer® D30 English (EN) 6.3 Method parameters This chapter illustrates the parameters for programming the methods. The order of the parameters in the device display may slightly differ from the order in the table in order to display the parameters more clearly. The table displays all parameters available for the various methods. Only a small portion of these parameters are required for the corresponding method and will be shown in the display.
Methods Eppendorf BioPhotometer® D30 English (EN) Parameter Entry Explanation Standards Value input: Number of standards. Range: 1 to 12. Number of different standard concentrations for the evaluation with standards. For some methods the range for the number of standards is restricted to a smaller range than 1 to 12. Replicates Value input: Number of replicates per standard. Range: 1 to 3. Number of repeated measurements for the various standard concentrations. Std. conc.
Methods Eppendorf BioPhotometer® D30 English (EN) Parameter Entry Explanation Autoprint Selection: on | off Printing a measuring result immediately following measurement with the thermal printer. Only the main result data will be printed. To output detailed data, the required data packets can be compiled and printed in the print & export method step at the end of a measuring series. 6.4 Method procedure Wizard: the wizard at the top of the display will take you through the method procedure.
Methods Eppendorf BioPhotometer® D30 English (EN) 6.4.1 Check parameters Softkeys • [Page dn] and [Page up]: Change between 1 to 3 parameter list pages. • [Edit]: Switch to the parameter edit mode. Editing mode for parameters: Modified parameters are marked with a red star until the modification has been saved. Softkeys • [Save] and [Save as]: Save changes. When using [Save as] you have to rename the method.
Methods Eppendorf BioPhotometer® D30 English (EN) 6.4.2 Measure standards The first standard to be measured is marked on the display. After the blank value (blank key) measure all standards (standard key) one by one. When measuring more than one replicate per standard, the average value for each standard is calculated and displayed automatically. By using the and cursor keys, you also can select certain standards for measurement. Individual standards can be remeasured as well.
Methods Eppendorf BioPhotometer® D30 English (EN) 6.4.3 Measure samples The sample key is used for measuring your samples consecutively. Blank results remain saved for one measuring series, but a new blank result measurement can be performed at any time. With the and keys you can navigate between the sample results that have been achieved in the measuring series up to this point. Results display: • The concentration result (6 digits with floating point) is clearly emphasized.
Methods Eppendorf BioPhotometer® D30 English (EN) Enter dilution The [Dilution] softkey is activated after the blank (blank key) has been measured. 1. Press the [Dilution] softkey. 2. Enter the volumes for the sample (up to 3 digits) and for the dilution buffer (up to 4 digits). The device will multiply the following sample results by the calculated dilution factor. Softkeys • [Clear dil.]: Delete values for sample dilution. • [OK]: Confirm sample dilution and return to sample measurement.
Methods Eppendorf BioPhotometer® D30 English (EN) Result image with dilution and ID Result image with dilution and sample ID 6.4.4 Measure samples: Results displays This section contains a display of typical results displays for all method groups and an overview of additional results data, which can be accessed using the [Data] softkey.
Methods Eppendorf BioPhotometer® D30 English (EN) Method group Nucleic acids Results display Explanation Results display: • Concentration result with absorbance at the measuring wavelength • If activated in the parameters: Ratios A260/A280 • If activated in the parameters: Restricted scan. Navigate between the measuring points on the graph which are used for the result calculation with and . Additional data ([Data] softkey):.
Methods Eppendorf BioPhotometer® D30 English (EN) Method group Results display Explanation Proteins (with reagent) Results display: • Concentration result with absorbance at the measuring wavelength. • For evaluation with standard series: graph of the standard evaluation with plotted sample result. Bacterial density Results display: • Calculated result with absorbance at the measuring wavelength.
Methods Eppendorf BioPhotometer® D30 English (EN) 6.4.5 Process results The sample measurement is followed by two optional steps in the method sequence: process results and print & export. In the process results step, you can convert the concentration results to molar concentrations or, after entering the volumes, into total amounts for the methods of the Nucleic acids group.
Methods Eppendorf BioPhotometer® D30 English (EN) After the changes have been saved you can apply them to all samples of the measuring series with [Yes]. 6.4.6 Print & export In the last optional method step, you can assemble data packets for all samples of a series of measurements, or selected samples of a series of measurements, for printing to the printer, export to a USB stick or export to a PC using a USB cable. Select data packets • Use the cursor keys for navigating and confirm with enter.
Methods Eppendorf BioPhotometer® D30 English (EN) Select export version If no USB stick is connected, the first variant cannot be selected. Export to USB stick 1. Connect a FAT 32-formatted USB stick to the USB port 4 (see Main illustration on p. 9). 2. Start with [Export] "export to an external storage medium". Export to PC Requirement for the PC operating system: Windows XP, SP2 or a higher version. 1. Connect the device to the PC by using the USB cable on the USB port 8 (see Main illustration on p.
Methods Eppendorf BioPhotometer® D30 English (EN) 6.4.7 Finish the series of measurements After the print & export method step has been finished, you can start a new series of measurements using the selected method or select a new method. Finish the series of measurements and start a new series of measurements • [Next >] softkey: Call up new series method step • [New] softkey: Call up measure samples method step and start a new series of measurements.
Methods Eppendorf BioPhotometer® D30 English (EN)
Functions Eppendorf BioPhotometer® D30 English (EN) 7 7.1 Functions Functions of the User main group With the function key or the [Function] softkey, you reach a menu containing functions like device settings or calling up saved results. The functions are structured in 3 columns analog to the method selection. The functions in the User main group are accessible to you.
Functions Eppendorf BioPhotometer® D30 English (EN) 7.1.1 Results memory In the right column, select the method for which you would like to call up saved results. Confirm with enter. Select the desired series of measurement with the cursor keys. Confirm with enter. As in the method procedure, you can also successively switch between the display of the parameters, standards, sample results and, finally, the data packets for print and export.
Functions Eppendorf BioPhotometer® D30 English (EN) If you would like to print or export results, select the data packets. The procedure for print and export and the meaning of the function keys corresponds to the print & export method step. 7.1.2 General method parameters In the right column, select the parameter group for which you would like to edit parameters. Confirm with enter. In this example, the parameter groups for different proteins are subsumed and stored respectively under a name.
Functions Eppendorf BioPhotometer® D30 English (EN) To edit a parameter group, use and to select the parameter which you would like to edit. Confirm with enter. Softkeys • [OK]: Save entry and return to the parameter group selection. • [Cancel]: Return to the parameter group selection without making any changes.
Functions Eppendorf BioPhotometer® D30 English (EN) • Specifications for proteins which are not preset at the factory can be determined in the expasy database: http://www.expasy.org/tools/protparam.html. • A table with A1% values for many proteins can also be found in: C.N.Pace et al., Protein Science (1995), 4: 2411–2423 (Table 5). The A1% values must be multiplied by 0.1 to return the required A0.1% values. 7.1.
Functions Eppendorf BioPhotometer® D30 English (EN) 7.1.5 Device calibration Information on checking the device is provided separately (see Checking the device on p. 54). 7.1.6 Info The Copyright menu item contains license information on the Open Source software.
Maintenance Eppendorf BioPhotometer® D30 English (EN) 8 8.1 Maintenance Cleaning DANGER! Electric shock as a result of penetration of liquid. Switch off the device and disconnect the power plug before starting cleaning or disinfection work. Do not allow any liquids to penetrate the inside of the housing. Do not spray clean/spray disinfect the housing. Only plug the device back in if it is completely dry, both inside and outside. NOTICE! Corrosion from aggressive cleaning agents and disinfectants.
Maintenance Eppendorf BioPhotometer® D30 English (EN) • Pull the cover to the right at a 90 degree angle. 3. Clean the cover with a cloth or lint-free cotton swab dampened with a mild cleaning agent. 4. Slide the locking pin back into the housing as far as it will go. The locking pin has completely disappeared in the housing. When the photometer is not being used, close the cuvette shaft using the blue cuvette shaft cover to protect it from dust and other contamination. 8.
Maintenance Eppendorf BioPhotometer® D30 English (EN) 8.3.1 Checking the photometer unit Eppendorf offers a filter kit for checking the photometric accuracy and the wavelength systematic error. The kit contains one blank filter A0 and 3 filters A1, A2 and A3 for checking the photometric accuracy as well as 2 filters for checking the wavelength systematic error (260 nm, 280 nm). The filter absorbances are measured in comparison with the blank filter A0.
Maintenance Eppendorf BioPhotometer® D30 English (EN) 8.3.1.1 Checking the photometric accuracy 1. In the Device calibration group, select the Photometer unit function and confirm with enter. 2. Select if you want to check the wavelength systematic error or the photometric accuracy. Confirm with enter. Press [Next >] to switch to the measurement. 3. Follow the instructions on the display and first measure the blank filter A0 and then the first test filter A1.
Maintenance Eppendorf BioPhotometer® D30 English (EN) 4. Result display after measuring a test filter for checking the photometric accuracy. Measure the other two test filters A2 and A3. 5. Results display after measuring all 3 test filters for testing the photometric accuracy. With the and keys, you can view again the results of the different test filters. Press [Finish] to complete the test. 6. Compare the average values and variation coefficients with the supplied table.
Maintenance Eppendorf BioPhotometer® D30 English (EN) 8.3.2 Device self test You can set the frequency of the automatic self test (taking approx. 1 minute) with the Device settings function (see Device settings on p. 51). Ex-factory, the self test interval is set to "weekly".
Maintenance Eppendorf BioPhotometer® D30 English (EN) 8.5 Decontamination before shipment If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following: WARNING! Risk to health from contaminated device 1. Follow the instructions in the decontamination certificate. You find it as a PDF file on our website (www.eppendorf.com/decontamination). 2. Decontaminate all the parts you would like to dispatch. 3.
Maintenance Eppendorf BioPhotometer® D30 English (EN)
Troubleshooting Eppendorf BioPhotometer® D30 English (EN) 9 9.1 Troubleshooting General errors Error Measuring results are imprecise. Possible cause Remedy • Reagent is past its shelf life. Ensure that the reagent is still within its shelf life and properly prepared. • Reagent has not been prepared properly. Use clean demineralized water of adequate quality for preparation if required. • Pipetting is not correct.
Troubleshooting Eppendorf BioPhotometer® D30 English (EN) Error Possible cause The measuring results are not correct. • The method has not been programmed correctly. Remedy Ensure that the method parameters are entered correctly. • The standard solution has Ensure that the correct standard is used not been prepared and that the measuring solution for the correctly. standard is prepared correctly. 9.2 • The absorbance of the reagent is drifting.
Troubleshooting Eppendorf BioPhotometer® D30 English (EN) Problem Cause Solution Blank measurement: An intensity on a pixel that influences the main, auxiliary or scan wavelength is too low. • The absorbance of the blank solution Check the blank solution and used for the blank measurement is too remeasure the blank if required. high. • Incorrect or turbid blank solution. The entered name is not valid. • Error when entering the name. Different causes are possible.
Troubleshooting Eppendorf BioPhotometer® D30 English (EN) Problem Cause Solution At least two of the entered standard concentrations are identical. Correct the standard concentrations. • See the error text. Enter the standard concentrations so that the first standard receives the lowest concentration and the other standard concentrations form an increasing sequence.
Troubleshooting Eppendorf BioPhotometer® D30 English (EN) 9.3 Result flags Warnings and error messages for results are displayed in the bottom right of the help box. The header bar of the Help box is highlighted yellow for warnings and red for error messages. Warnings: Decide whether the result is useful for you while taking the displayed warning into consideration. Error messages: No result is displayed; the reason is shown in the error message.
Troubleshooting Eppendorf BioPhotometer® D30 English (EN) Problem Cause Solution The coefficient of determination for the regression evaluation of the standard series is < 0.8. • For methods with evaluation of Use the sample results with the standard series via the regression reservation mentioned or repeat the procedure: If the regression measurement of the standard series evaluation for the standard series was and samples.
Transport, storage and disposal Eppendorf BioPhotometer® D30 English (EN) 10 10.1 Transport, storage and disposal Transport Use the original packaging for transport.
Transport, storage and disposal Eppendorf BioPhotometer® D30 English (EN)
Technical data Eppendorf BioPhotometer® D30 English (EN) 11 11.1 Technical data Power supply Power supply 100 V to 240 V ±10 %, 50 Hz to 60 Hz Overvoltage category II Degree of pollution 2 Power consumption Maximum power consumption according to name plate: 25 W Approx. 15 W during operation Approx. 5 W with the display dimmed Permitted mains interruption Approx. 10 ms at 90 V Approx. 20 ms at 230 V Protection class I Fuses T 2.5 A/250 V, 5 mm × 20 mm (2 pcs.) 11.
Technical data Eppendorf BioPhotometer® D30 English (EN) 11.
Technical data Eppendorf BioPhotometer® D30 English (EN) 11.6 Application parameters Methods Preprogrammed and freely programmable methods for all measuring and evaluation procedures: • Absorbance measurements • Nucleic acids and proteins, OD600 • Methods with evaluation via factor, standard and standard series Method-dependent evaluation Absorbance, concentration via factor and standard.
Technical data Eppendorf BioPhotometer® D30 English (EN)
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN) 12 Evaluation procedure This chapter describes the evaluation procedures available in the method programs as well as the calculation of a dilution using the device software. When comparing the measuring results to the results of other photometers/ spectrophotometers, note that the values may be dependent on the bandwidth of the devices.
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN) 12.1.3 Cuvette correction All absorbance values which are used for result calculation are standardized to the cuvette layer thickness of 10 mm. If a cuvette with a different path length is used, this path length must be defined in the cuvette parameter. In this case, the measured absorbances are corrected to match measuring results with a cuvette layer thickness of 10 mm before converting them to sample results.
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN) The factor is either entered directly as a parameter during the "Factor" evaluation procedure or calculated during the "Standard" evaluation procedure (evaluation with a standard concentration): F CS AS F = calculated factor CS = concentration of the standard (enter as parameter). AS = measured absorbance of the standard.
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN) • Use the "linear regression" procedure for calibration lines. • With curvilinear gradients, test which evaluation procedure (quadratic regression, cubic regression, spline interpolation) produces the function that is most suitable to the standard evaluation.
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN) 12.5 Special evaluation procedures for nucleic acids and protein UV This section covers the evaluation of nucleic acids or proteins in the Nucleic acids and Proteins direct UV method groups. 12.5.1 Ratios A260/A280 and A260/A230. Application: Information on the purity of the measured nucleic acid. Application of the evaluation procedure can be activated in the A260/280 or A260/A230 parameters.
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN) 12.5.2.2 Calculation of the molar concentration Application: calculation of the molar concentration of the nucleic acid from the mass concentration and relative molar mass. The molar mass is either entered directly or calculated by the device from the entered number of bases or base pairs per nucleic acid molecule. CMol C u 103 MM CMol = calculated molar concentration of the nucleic acid. Unit: pmol/mL.
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN) 12.5.3 Calculating the factor for protein in "General Method Parameter" This section only covers the calculation of the protein component in the Proteins direct UV method group. For this method group, the protein component is selected in the parameters (see Method parameters on p. 32). The protein component is assigned a factor that will be entered in the General Method Parameter/ Proteins function for each protein. Alternatively, A0.
Evaluation procedure Eppendorf BioPhotometer® D30 English (EN)
Ordering information Eppendorf BioPhotometer® D30 English (EN) 13 Ordering information Order no. (International) Order no. (North America) 6133 000.001 – 6133 000.010 6133000010 6133 000.907 6133 000.908 6133000907 6133000908 6133 928.004 6133928004 6135 011.000 6135 010.004 6135 012.007 6135010004 0013 021.566 952010409 6138 000.018 6138000018 0030 106.300 952010051 0030 106.318 952010069 0030 079.345 0030079345 0030 079.353 0030079353 4308 078.
Ordering information Eppendorf BioPhotometer® D30 English (EN)
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