vanced® or EN) nsusefor use Combitips advanced® Instructions for use
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. Eppendorf, Multipette and Repeater, Combitips advanced, Biopur are registered trademarks of Eppendorf AG, Hamburg, Germany. Registered trademarks are not marked in all cases with ™ or ® in this manual. 0012 581.
Table of contents Combitips advanced® English (EN) Table of contents 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1.2 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Operating instructions Combitips advanced® English (EN) 1 1.1 Operating instructions Using this manual Please read these instructions for use completely before using the Combitips advanced for the first time. Also read the operating manual of the dispenser used. 1.2 Symbols used Symbol Meaning You are requested to perform an action. 1. 2. Perform these actions in the sequence described. • List. References useful information. 1.
Operating instructions Combitips advanced® English (EN) E Eppendorf Quality Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness. G Graduation Incremental graduation of a range, a surface or a volume. M Maximum volume The maximum volume that can be used for dispensing.
Operating instructions Combitips advanced® English (EN) S Sterile Sterile Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with regard to sterility and freedom from pyrogens. Systematic error Accuracy. Describes how close the average value of several measurements of the same volume is to the actual value.
Product description Combitips advanced® English (EN) 2 2.1 Product description Main illustration ^ 1=50µL 1 2,5 mL 2 4 3 5 6 7 1 Dispensing volume with the manual dispenser 5 Color code at selection dial position 1 6 Coding 2 Graduation 7 Piston 3 US patent numbers 4 Maximum volume 2.2 Overview of Combitips advanced with color codes ^ 0.1 mL 0.2 mL 0.5 mL 2.3 1 mL 2.
Product description Combitips advanced® English (EN) 2.4 Compatible devices The Combitips advanced can be used with the following Eppendorf dispensers: Dispenser Dispensing range Multipette 4780/Repeater 4780 2 μL – 5 mL Multipette plus/Repeater plus 1 μL – 10 mL Multipette pro/Repeater pro 1 μL – 50 mL Multipette stream/Repeater stream 1 μL – 50 mL Multipette Xstream/Repeater Xstream 1 μL – 50 mL EDOS 5222 1 μL – 50 mL 2.
Product description Combitips advanced® English (EN) 2.6 Evaluation criteria The Combitips advanced can be used for the single dispensing of all chemicals which are included in the following tables. ■■■ Resistant The chemical can be used. ■■ Limited resistance and/or suitable for limited use The chemical can be used for a limited period of time. Dispensing must be performed soon after filling in order to ensure the tightness of the Combitips advanced and to avoid damage to the dispenser.
Product description Combitips advanced® English (EN) 2.7 Resistance to chemicals Acids and bases Concentration in % Resistance Ammonia solution 25 ■■■ Ammonia solution 2.0 ■■■ Acetic acid 96 ■■■ Acetic acid 12 ■■■ Caustic soda 20 ■■■ Caustic soda 4.0 ■■■ Perchloric acid 10 ■■■ Nitric acid 65 ■■ Nitric acid 6.3 ■■■ Hydrochloric acid 32 ■■■ Hydrochloric acid 3.
Safety Combitips advanced® English (EN) 3 3.1 Safety Intended use Combitips advanced are intended to be used with a Multipette/Repeater or a EDOS 5222 for dispensing liquids in the 1 μL – 50 mL volume range. In-vivo applications (applications in or on the human body) are not permitted. The Combitips advanced may only be used by specialized staff who have been adequately trained.
Safety Combitips advanced® English (EN) WARNING! Personal injury and material damage from a bursting Combitip. If stored for a long period of time, liquids can crystallize or solidify and thus block the outlet opening. The Combitip advanced may burst during the process of dispensing. Check the consistency of the liquid. Dispense immediately after liquid aspiration.
Operation Combitips advanced® English (EN) 4 4.1 Operation Unpacking To ensure maximum protection from carry-over, use Combitips advanced with Sterile and Biopur grades of purity immediately after they have been removed from their packing. 1. Open the packing at the location indicated. 2. Insert the Combitip into the dispenser. 3. Insert the Combitip into the dispenser.
Operation Combitips advanced® English (EN) 4.2 Assembling the Combitip advanced and Adapter advanced Combitips advanced with a maximum volume of 0.1 mL – 10 mL can be used immediately. Combitips advanced with a maximum volume of 25 mL and 50 mL can only be used with the corresponding Adapter advanced. Adapter advanced and Combitips advanced have the same color code. The maximum volume is also listed on the neck of the Adapter advanced.
Operation Combitips advanced® English (EN) 4.3 Inserting the Combitip NOTICE! Device damage due to incorrect handling of the inserted Combitip Insert the Combitip straight into the dispenser from below. Do not rotate the inserted Combitip. Never grip the dispenser using the Combitip. The Combitip is easier to insert if the ejector is held pressed down during insertion. 1 4.4 2 1. If the Combitip piston has been pushed out, push it back into the cylinder of the Combitip. 2.
Maintenance Combitips advanced® English (EN) 5 5.1 Maintenance Cleaning Combitips advanced are not autoclavable. Adapter advanced and Combitips advanced Rack are autoclavable. 5.1.1 Autoclaving the Adapter advanced Rinse the Adapter advanced with water. Autoclave the Adapter advanced for 20 min at an overpressure of 1 bar and 121 °C. The Adapter advanced can be autoclaved up to 100 times. 5.1.
Technical data Combitips advanced® English (EN) 6.2 Errors with the Multipette M4/Repeater M4 Combitip advanced Testing volume Error limits Error Systematic error Random error ±% ± µL ±% ± µL 0.1 mL white Increment: 1 μL 2 μL ±1.6 ±0.032 ±3.0 ±0.06 20 μL ±1.0 ±0.2 ±2.0 ±0.4 0.2 mL light blue Increment: 2 μL 4 μL ±1.3 ±0.052 ±2.0 ±0.08 40 μL ±0.8 ±0.32 ±1.5 ±0.6 0.5 mL purple Increment: 5 μL 10 μL ±0.9 ±0.09 ±1.5 ±0.15 100 μL ±0.8 ±0.8 ±0.6 ±0.
Technical data Combitips advanced® English (EN) Test conditions and test evaluation in compliance with ISO 8655, Part 6. Analytical balance with evaporation protection inspected by the office of weights and measures.. • Number of determinations: 10 • Use of water in accordance with ISO 3696 • Inspection at 20 °C – 25 °C ±0.
Technical data Combitips advanced® English (EN) 6.3 Errors with the Multipette plus/Repeater plus Combitip advanced Volume range Error limits Error Systematic error Random error ±% ± µL ±% ± µL 2 μL ±1.6 ±0.032 ±3.0 ±0.06 20 μL ±1.0 ±0.2 ±2.0 ±0.4 4 μL ±1.3 ±0.052 ±2.0 ±0.08 40 μL ±0.8 ±0.32 ±1.5 ±0.6 10 μL ±0.9 ±0.09 ±1.5 ±0.15 100 μL ±0.8 ±0.8 ±0.6 ±0.6 20 μL ±0.9 ±0.18 ±0.9 ±0.18 200 μL ±0.6 ±1.2 ±0.4 ±0.8 50 μL ±0.8 ±0.4 ±0.8 ±0.4 500 μL ±0.5 ±2.
Technical data Combitips advanced® English (EN) 6.4 Errors with the Multipette (X)stream/Repeater (X)stream Combitip advanced Volume range Testing volume Error limits Error Systematic error Random error ±% ± µL ±% ± µL 10 μL ±1.6 ±0.16 ±2.5 ±0.25 50 μL ±1.0 ±0.5 ±1.5 ±0.75 100 μL ±1.0 ±1.0 ±0.5 ±0.5 20 μL ±1.3 ±0.26 ±1.5 ±0.3 100 μL ±1.0 ±1.0 ±1.0 ±1.0 200 μL ±1.0 ±2.0 ±0.5 ±1.0 50 μL ±0.9 ±0.45 ±0.8 ±0.4 250 μL ±0.9 ±2.25 ±0.5 ±1.25 0.
Technical data Combitips advanced® English (EN) Test conditions and test analysis in accordance with ISO 8655, part 6. Test with an analytical balance with a moisture trap which has been inspected by the Office of Weights and Measures. • Number of determinations: 10 • Use of water in accordance with ISO 3696 • Inspection at 20 °C – 25 °C ± 0.
Technical data Combitips advanced® English (EN) 6.5 Adjustable dispensing volumes Applies to following dispensers: • Multipette M4/Repeater M4 • Multipette plus/Repeater plus Selec- Dispens- 0.1 mL tion ing steps dial white 0.2 mL 0.5 mL 1.0 mL 2.5 mL 5.0 mL 10 mL 25 mL 50 mL light blue purple yellow green blue orange red light gray ● 100 1.0 μL 2.0 μL 5.0 μL 10 μL 25 μL 50 μL 0.1 mL 0.25 mL 0.5 mL 1 50 2.0 μL 4.0 μL 10 μL 20 μL 50 μL 100 μL 0.2 mL 0.50 mL 1.
Purity grades and certificates Combitips advanced® English (EN) 7 7.1 Purity grades and certificates Eppendorf purity grades Eppendorf Quality Sterile Human DNA-free PCR clean Biopur ■ ■ DNA-free (Human + Bacteria DNA-free) ■ DNase-free ■ ■ RNase-free ■ ■ PCR inhibitor-free ■ ■ ATP-free ■ Pyrogen-free (Endotoxin-free) ■ ■ Sterile (Ph.Eur./USP) ■ ■ 7.2 Certificates Various certificates for Eppendorf consumables can be downloaded from our website www.eppendorf.de/ consumables.
Ordering Information Combitips advanced® English (EN) 8 8.1 Ordering Information Combitips advanced Order no. (International) 0030 089.405 – 0030 089.618 0030 089.766 0030 089.413 – 0030 089.626 0030 089.774 0030 089.421 – 0030 089.634 0030 089.782 0030 089.430 – 0030 089.642 0030 089.790 0030 089.448 – 0030 089.650 0030 089.804 0030 089.456 – 0030 089.669 0030 089.812 Order no. (North America) Description 0030089405 0030089510 0030089618 – Combitips advanced 0.
Ordering Information Combitips advanced® English (EN) Order no. (International) Order no. (North America) Description 0030089464 0030089570 0030089677 – Combitips advanced 10 mL 100 pieces Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean 0030089472 0030089588 0030089685 – Combitips advanced 25 mL 100 pieces + 4 Adapter Eppendorf Quality Sterile, individually wrapped Biopur, individually wrapped PCR clean 0030 089.480 – 0030 089.693 0030 089.
Ordering Information Combitips advanced® English (EN) 8.2 Accessories anced Combitips adv Combilong/Combitube The Combilong/Combitube is an aspiration tool for the Combitips advanced. It enables liquids to be directly taken out of all bottles. Order no. (International) 0030 059.506 – 0030 089.758 Order no. (North America) ® Combitips advanced Rack A Combitips advanced Rack is available for storing the Combitips advanced (≤ 10 mL).
Ordering Information Combitips advanced® English (EN) 8.2.1 Multipette M4/Repeater M4 Order no. (International) Order no. (North America) Description 4982 000.012 – Multipette M4 – 4982000020 Repeater M4 4982 000.314 – Multipette M4 Starter Kit Multipette M4, Combitip Rack, Combitip Assortmentpack – 4982000322 Repeater M4 Starter Kit Repeater M4, Combitip Rack, Combitip Assortmentpack 8.3 Multipette plus/Repeater plus Order no. (International) Order no.
Transport, storage and disposal Combitips advanced® English (EN) 9 9.1 Transport, storage and disposal Storage NOTICE! Damage due to UV radiation Do not store consumables in areas with strong UV radiation.
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