Manual

97
Evaluation procedure
Eppendorf BioSpectrometer
®
kinetic
English (EN)
12.6.3 Conversion to amounts of dye
The quantity (dimensions) of dye in the entire sample volume is calculated in the process results/More
calculations method step.
M = calculated total amount (mass) of dye in the sample tube. Unit: pmol.
C = dye concentration calculated from the measurement. Unit: pmol/μL.
V
S, total
= total volume of the sample in the sample tube; entered by the user under More calculations. 
Unit: μL.
12.7 Dual wavelength
For methods of the Dual Wavelength group absorbances that were measured at two wavelengths can be
calculated with each other before the calculated absorbance is evaluated further with the factor or
standard.
To determine the calculated absorbance a division or subtraction evaluation can be defined in the
parameters:
A
1
, A
2
= measured absorbance.
a, b, c, d = factors that are entered in the parameters. Negative figures may also be entered.
totalP
VCM
,
u
dc
Ab
Aa
A
calc
u
u
u
2
1

>@ dcAbAaA
calc
uuu
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