Manual

Technical data

Eppendorf BioSpectrometer

kinetic

English (EN)

11.4 Photometric properties

11.5 Incubation

Measuring principle Single beam absorption spectrophotometer with reference beam

Light source Xenon flash lamp

Monochromator Holographic aberration-corrected concave grating

Beam receiver CMOS photodiode array

Wavelengths 200 nm to 830 nm

Wavelength selection Method-dependent, freely selectable

Spectral bandwidth ≤ 4 nm

Smallest step size 1 nm

Systematic wavelength error ±1 nm

Random wavelength error ≤ 0.5 nm

Photometric measuring range 0 A to 3.0 A at 260 nm

Reading accuracy ΔA = 0.001

Random photometric error ≤ 0.002 at A = 0

≤ 0.005 (0.5 %) at A = 1

Systematic photometric error ±1 % at A = 1

Stray light component < 0.05 %

Adjustable temperature range 20°C to 42°C

Smallest step size 0.1°C

Maximum temperature

uncertainty (in relation to the

temperature in the sample)

(see Tab. on p. 27)

Systematic temperature error ±0.2 °C at 25 °C to 37 °C

Random temperature error ±0.15 °C at 25 °C to 37 °C