Manual

81
Troubleshooting
Eppendorf BioSpectrometer
®
kinetic
English (EN)
The coefficient of
determination for
the regression
evaluation of the
standard series is
< 0.8.
For methods with evaluation of standard
series via the regression procedure: If the
regression evaluation for the standard
series was nonlinear, but the standard
evaluation was accepted by the user, a
warning appears after samples have been
measured.
Use the sample results with the
reservation mentioned or repeat
the measurement of the standard
series and samples.
Scan: Some of the
measured
absorbances are
too high and are
not displayed.
For at least one scan wavelength, the
absorbance exceeded the measuring
range.
Turbidity of the measuring solution.
Measurements at the limits of the
photometric measuring range.
If the non-displayed areas of the
scan are relevant: Dilute the
sample or remove the dilution via
centrifugation and repeat the
measurement.
The measurement
is not complete.
Kinetic procedure: You have prematurely
canceled the measurement using the
[Stop] softkey. If at least 2 measuring
points are available, a result is calculated
and displayed.
Accept the measuring result with
the reduced measuring time or
measure again with a longer
measuring time.
The kinetics are
nonlinear: The
coefficient of
determination is
< 0.95.
Kinetic procedure with "linear
regression" measuring procedure: The
coefficient of determination for the
regression evaluation indicates a
significant deviation of the measuring
points from the regression line.
Turbidity of the measuring solution.
Measurements at the limits of the
photometric measuring range.
Activity concentration of the enzyme too
high.
Accept measuring result or
remeasure the sample. Before
repeating the measurement,
evaluate the reason for the
non-linearity and act accordingly
(e.g., clear the measuring solution
by centrifugation or dilute the
sample).
The kinetics are
nonlinear for at
least one standard:
The coefficient of
determination is
<0.95.
Kinetic procedure with "linear
regression" measuring procedure and
evaluation procedure via standards: The
coefficient of determination for the
regression evaluation of at least one
standard measurement indicates a
significant deviation of the measuring
points from the regression line.
Turbidity of the measuring solution.
Measurements at the limits of the
photometric measuring range.
Activity concentration of the enzyme too
high.
Accept measuring result or
remeasure the standard. Before
repeating the measurement,
evaluate the reason for the
non-linearity and act accordingly
(e.g., clear the measuring solution
via centrifugation or use the
standard with low concentration).
Problem Cause Solution