Manual

Functions
Eppendorf BioSpectrometer
®
kinetic
English (EN)
62
When programming a method of the Dye labels or Proteins direct UV method groups, you can access the
entries in General Method Parameter:
Tab. 7-2: Parameter in General Method Parameter
Select the name of the dye to import the
corresponding parameter group into the method
program. By using the "edit" selection of the
"Nucleic acid" parameter, you also can get directly
to the General Method Parameter function and
view and edit the parameters.
Parameter Explanation
Proteins These parameters are loaded into the method parameters when a protein is
selected during the programming of a method in the Dye labels and Proteins
direct UV groups.
• Protein name
•Factor
•A
0.1%
•Ext.coeff.
•Molecular mass
In order to define a factor for calculating the concentration on the basis of the
absorbance, enter the following data in addition to the name and wavelength:
Factor or A
0.1%
or absorbance coefficient and molar mass.
Nucleic acids These parameters are loaded into the method parameters when a nucleic acid
is selected during the programming of a method in the Dye labels group.
•NA name
•Factor
• Double-stranded
The factor is used to calculate the concentration on the basis of the
absorbance.
The double-stranded parameter affects the calculation of the molar nucleic
acid concentration. (see Conversion to molar concentrations and nucleic acid
quantities on p. 94)
Dyes These parameters are loaded into the method parameters when a dye is
selected during the programming of a method in the Dye labels group.
•Dye name
• Wavelength
•Ext.coeff.
•Factor
• Corr. A260
• Corr. A280
In order to define a factor for calculating the concentration on the basis of the
absorbance, enter the following data in addition to the name:
Factor or absorbance coefficient.
The correction factors for the absorbance values at 260 or 280 nm are used
when the correction function in the method parameters is active. For more
details, refer to the chapter on evaluation (see Correction A
260
and correction
A
280
on p. 93).
Units You can select a unit from all available units when programming method
parameters.
• Unit Entering a unit that has not yet been programmed for the concentration result.