Owner's manual

Technical data

Eppendorf BioSpectrometer

fluorescence

English (EN)

11.4 Photometric properties

11.5 Fluorimeter

Measuring principle Single beam absorption spectrophotometer with reference beam

Light source Xenon flash lamp

Monochromator Holographic aberration-corrected concave grating

Beam receiver CMOS photodiode array

Wavelengths 200 nm to 830 nm

Wavelength selection Method-dependent, freely selectable

Spectral bandwidth ≤ 4 nm

Smallest step size 1 nm

Systematic wavelength error ±1 nm

Random wavelength error ≤ 0.5 nm

Photometric measuring range 0 A to 3.0 A at 260 nm

Reading accuracy ΔA = 0.001

Random photometric error ≤ 0.002 at A = 0

≤ 0.005 (0.5 %) at A = 1

Systematic photometric error ±1 % at A = 1

Stray light component < 0.05 %

Measuring principle Confocal filter fluorimeter with reference beam

Light source LED

Monochromator Filter arrangement consisting of dichroic and long pass filters

Beam receiver Photodiode

Excitation wavelength 470 nm

bandwidth: 25 nm

Emission wavelength I 520 nm

bandwidth: 15 nm

Emission wavelength Ii 560 nm

bandwidth: 40 nm

Measuring range 0.5 nM to 1 000 nM fluorescein (emission wavelength of 520 nm)

Random error of the

fluorimeter

±2 % at 1 nM fluorescein (emission wavelength of 520 nm)