Owner's manual

Methods
Eppendorf BioSpectrometer
®
fluorescence
English (EN)
32
Tab. 6-2: Fluorimetric methods
You can create new methods in all folders using <New Method>.
In Favorites, you can create your own folders (e.g., to allocate folders to specific people), and rename and
delete the folders.
Tab. 6-3: Softkeys in method selection
Copied or cut methods can be added to a different folder under Favorites, or added to the original folder
under a new name. Use the cursor keys to navigate to the Methods column of the desired folder and press
[paste] for adding the method.
6.2 Photometry method description
The preprogrammed methods and method templates are described in this section.
6.2.1 Absorbance method group
Single λ
• Absorbance measurement on a wavelength.
• No subsequent evaluation.
Multi λ
• Absorbance measurement at two to six wavelengths.
• No subsequent evaluation.
Scan
• Absorbance wavelength spectra measurement via a defined wavelength range.
• Display of wavelength and absorbance in the spectrum by navigation with a wavelength cursor.
• The spectra section can be modified using 3 different zoom variants.
• Peak detection possible.
Routine Fluorimetric nucleic acid and protein measurements with reagents
supplied by Invitrogen. (Carrying out these procedures may require a
license from Molecular Probes, Inc., Eugene, OR, USA or Invitrogen
Corporation, Carlsbad, CA, USA.)
Basic • Methods for the evaluation of fluorescence measurements with
standard or standard curve/line.
• Raw fluorescence method for rapid measurement of fluorescence
without further evaluation.
[Cut] and [Paste] Cut and paste methods.
[Copy] and [Paste] Copy and paste methods.
[Delete] Delete methods.
[Rename] Rename methods.