Manual

51
2 Technical data
Photometer
Optical system: Absorption single-beam photometer with reference beam
and several fixed wavelengths
Irradiation source: Xenon flash lamp
Spectral dispersion: Holographic concave grating
Measuring wavelengths: Xe 230, 260, 280, 320, 562, 595 nm
Wavelength selection: Method-dependent, program-controlled
Spectral bandwidth: 5 nm at 230 to 320 nm
7 nm at 562 to 595 nm
Wavelength systematic error: ± 1 nm at 230 to 280 nm
± 2 nm at 320 to 595 nm
Wavelength random error: ≤ 0.1 nm
Photometric measuring range: Quartz glass cuvette: 0.000 to 3.000 A
UVette
®
(Eppendorf): 2.5 A at 230 nm
2.6 A at 260 nm
2.8 A at 280 nm
2.9 A at 320 nm
Photometric random error: ≤ 0.002 A at 0 A
≤ 0.005 A at 1 A
Photometric systematic error: ± 1 % at 1 A
Accuracy of reading: 0.001 A
Stray-light proportion: < 0.05 %
Radiation detector: Silicium photo diodes
Measuring procedures
Measuring procedure: End-point against blank
Method-dependent
calculation: Absorbance
Concentration via factor
Concentration via Warburg formula
Concentration via calibration with 1 to 10 standards
One-point calibration (1 standard)
Linear regression (2 to 10 standards)
Non-linear regression
(3rd degree polynomer; 4 or 5 to 10 standards;
see Section 12, "Calculation")
1 x, 2 x or 3 x determination
For nucleic acids:
Ratio 260/280
Ratio 260/230
Molar concentration
Total yield
Memory
Method memory: 12 preprogrammed, modifiable method programs
Calibration memory: For all calibration procedures
Results memory: For 100 results with absorbance and ratio values,
sample number, sample dilution, date and time (calendar up to 2090)
2 Technical data
02_TecDat_e.fm Seite 51 Montag, 20. Februar 2006 16:06 Uhr