Manual

83
10 Short instructions
Measuring procedure for dsDNA
dsDNA
PROGRAMMED FACTOR
1 A260 = 50.0 µ g/mL
Sample
dsDNA BLANK
Blank
dsDNA SAMPLE 001
20+200 µL
0 . 0 0 0 A
CALC: AMOUNT:
TOTAL SAMPLE 140 µ L
CALC: MOLARITY:
BASE PAIRS 300
MOL.MASS 198 kDa
dsDNA SAMPLE 001
20+200 µL 0.694 A
230
1.408 A260
1.97 260/280 0.715 A280
2.03 260/230 0.002 A320
5 6 3 . 2 0 µg/mL
dsDNA SAMPLE 001
20+200 µL
79 µg
2843 pmol/mL
398 pmol
5 6 3 . 2 0 µg/mL
dsDNA SAMPLE 002
20+200 µL 0.689 A230
0.788 A260
1.85 260/280 0.623 A280
2.19 260/230 0.003 A320
2 4 9 . 7 0 µg/mL
Select dsDNA method
Measure blank
Enter
Enter
2
Lowry
Dilution
0
Sample No.
0
Sample No.
0
Sample No.
When the sample is diluted:
Example: 20 + 200 µL
2
Lowry
Measure sample
If the result of the sample
is to be converted:
Enter
Enter
Enter

Conversion
0
Sample No.
0
Sample No.
0
Sample No.
1
Bradford
4
Protein
3
BCA
Measure next sample
Sample
Sample
7
dsDNA
Blank
Blank
Sample
or
or
Sample
Blank
or
10_Kurz_e.fm Seite 83 Montag, 20. Februar 2006 11:33 Uhr