User Manual

Maintenance

Eppendorf

PlateReader AF2200

English (EN)

8.6.4 Absorbance test instructions - baseline stability

Conduct the following measurements to determine the baseline stability using the available filters.

Calculating the baseline stability

Calculate the standard deviation for each wavelength over 20 cycles.

Checking the accuracy of the absorbance

Parameter Setting

Measuring mode Absorbance

Wavelength 340 nm

405 nm

492 nm

590 nm

620 nm

700 nm

Number of flashes 25

Setting time 0 (µs)

Part of plate A1

Kinetic cycles 20, minimum time interval

• No plate is required for the measurement – the plate compartment must be empty for this

measurement.

• No materials or reagents are needed for this test.

Contact Eppendorf Service to have the accuracy of the absorbance checked.