User Manual

Maintenance

Eppendorf

PlateReader AF2200

English (EN)

Precision from one measurement to the next

Carry out the following measurement to determine the precision and reproducibility.

Pipette 200 µL of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells

according to the plate layout below.

Material/reagents

• 1 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)

• 0.01 M NaOH (=blank solution) (NaOH pellets)

• 96-well plate

• 200 µL pipette with tips

Parameter Setting

Measuring mode Upper fluorescence

Wavelength, excitation 485 (20 nm)

Wavelength, emission 535 (25 nm)

Number of flashes 25

Integration time 40 (µs)

Setting time 0 (µs)

Signal gain Optimal

Plate type GRE96fb

Part of plate A1

Kinetic 20 cycles

Time interval Minimum

X 1 nM fluorescein

blank 0.01 M NaOH