User Manual

Maintenance

Eppendorf

PlateReader AF2200

English (EN)

8.6.2 Test instructions, upper fluorescence

The following tests can be conducted on the device to check the device specifications for this measuring

procedure:

• Sensitivity

• Precision within a plate

• Precision from one measurement to the next

Sensitivity

Conduct the measurement with the following settings to determine the detection limit for fluorescein.

Pipette 200 µL of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells

according to the plate layout below.

Parameter Setting

Measuring mode Upper fluorescence

Wavelength, excitation 485 (20 nm)

Wavelength, emission 535 (25 nm)

Number of flashes 25

Integration time 40 (µs)

Setting time 0 (µs)

Signal gain Optimal

Plate type GRE96fb

X 1 nM fluorescein

blank 0.01 M NaOH